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Insects

Insects is an international, peer-reviewed, open access journal on entomology published monthly online by MDPI.

Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
High Visibility: indexed within Scopus, SCIE (Web of Science), PubMed, PMC, PubAg, and other databases.
Journal Rank: JCR - Q1 (Entomology) / CiteScore - Q1 (Insect Science)
Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 18.1 days after submission; acceptance to publication is undertaken in 2.9 days (median values for papers published in this journal in the first half of 2025).
Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
Journal Cluster of Animal Science: Animals, Arthropoda, Birds, Insects, Journal of Zoological and Botanical Gardens, Pets, Poultry, Ruminants and Veterinary Sciences.

Impact Factor: 2.9 (2024); 5-Year Impact Factor: 3.3 (2024)

Imprint Information Journal Flyer Open Access ISSN: 2075-4450

Latest Articles

10 pages, 361 KiB

Open AccessArticle

Effects of Photoperiod on the Developmental Duration and Reproduction of Sclerodermus sichuanensis

by Kui Kang, Lina Wang, Zhongjiu Xiao, Shaobo Wang, Ke Wei, Xiaoyi Wang, Yanlong Zhang and Yanlong Tang

Insects 2025, 16(7), 701; https://doi.org/10.3390/insects16070701 - 8 Jul 2025

Sclerodermus sichuanensis, a parasitic wasp, plays a significant role in wood-boring forest pest control in China. Research has shown that the photoperiod significantly affects the development and reproduction of parasitic wasps. However, the effects of the photoperiod on S. sichuanensis have not [...] Read more.

Sclerodermus sichuanensis, a parasitic wasp, plays a significant role in wood-boring forest pest control in China. Research has shown that the photoperiod significantly affects the development and reproduction of parasitic wasps. However, the effects of the photoperiod on S. sichuanensis have not yet been reported. This study investigates the impact of different photoperiods on the developmental duration and reproduction of S. sichuanensis. The wasps were reared under four photoperiod conditions: 0L:24D, 8L:16D, 16L:8D, and 24L:0D. The results show that increased light duration shortened the egg, larval, and pupal stages, with the total developmental period decreasing by up to 17 days. The number of offspring was highest in the 8L:16D and 16L:8D treatments, while constant light (24L:0D) led to a significant decrease in offspring numbers. The parasitism rate and pre-oviposition period were also affected by light exposure, with the longest pre-oviposition period observed in complete darkness. These findings indicate that the photoperiod plays a crucial role in regulating the development and reproductive efficiency of S. sichuanensis, suggesting that appropriate light conditions could enhance the efficiency of its use in pest control. Full article

(This article belongs to the Section Insect Physiology, Reproduction and Development)

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14 pages, 5582 KiB

Open AccessArticle

Silencing Miniature Gene Disrupts Elytral and Hindwing Structures in Leptinotarsa decemlineata

by Man-Hong Cheng, Kai-Yun Fu, Wei Zhou, Ji-Feng Shi and Wen-Chao Guo

Insects 2025, 16(7), 700; https://doi.org/10.3390/insects16070700 - 8 Jul 2025

The Colorado potato beetle (Leptinotarsa decemlineata, CPB) is a major pest in potato crops, notorious for its rapid dispersal and insecticide resistance, which are enabled by its robust elytra and flight-capable hindwings. The Miniature (Mi) gene, encoding a protein [...] Read more.

The Colorado potato beetle (Leptinotarsa decemlineata, CPB) is a major pest in potato crops, notorious for its rapid dispersal and insecticide resistance, which are enabled by its robust elytra and flight-capable hindwings. The Miniature (Mi) gene, encoding a protein with a zona pellucida (ZP) domain, is involved in wing development and cuticle integrity, yet its functional role in beetles remains underexplored. In this study, we cloned and characterized the LdMi gene in the CPB and investigated its function using RNA interference (RNAi), morphological analyses, and spectroscopy. LdMi encodes a 146.35 kDa transmembrane protein with a conserved ZP domain, clusters with coleopteran homologs, and exhibits relative conservation across insect species. Expression profiling showed high LdMi transcript levels in the hindwings, the elytra, and the pupal stages. RNAi knockdown in fourth-instar larvae resulted in severe eclosion defects, including malformed wings and reduced adult weight. Scanning electron microscopy (SEM) revealed disrupted elytral patterns and deformed hindwing veins in knockdown individuals. Spectroscopic analyses using Fourier-transform infrared (FTIR) and Raman spectroscopy indicated a reduction in protein–chitin crosslinking and diminished hydrogen bonding, suggesting compromised cuticular integrity. These results highlight the essential role of LdMi in cuticle formation and the surface morphology of the elytra and hindwings, offering new insights into ZP domain proteins in insects. Full article

(This article belongs to the Special Issue RNAi in Insect Physiology)

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20 pages, 4119 KiB

Open AccessArticle

Insights into Lead Toxicity and Detoxification Mechanisms in the Silkworm, Bombyx mori

by Dan-Dan Bian, Yan-Xia Shi, Kai-Wen Shi, Hui-Cong Du, Bo-Ping Tang and Qiu-Ning Liu

Insects 2025, 16(7), 699; https://doi.org/10.3390/insects16070699 - 7 Jul 2025

Bombyx mori, a key lepidopteran model with economic importance, is highly susceptible to environmental heavy metal pollution. This study investigated the mechanisms of Pb toxicity and the associated detoxification and metabolic defense responses in silkworms, employing transcriptome sequencing, enzyme activity assays, and [...] Read more.

Bombyx mori, a key lepidopteran model with economic importance, is highly susceptible to environmental heavy metal pollution. This study investigated the mechanisms of Pb toxicity and the associated detoxification and metabolic defense responses in silkworms, employing transcriptome sequencing, enzyme activity assays, and histopathological analysis. Pb exposure caused significant histopathological changes and apoptosis in the fat body, marked by structural disorganization, swollen adipocytes, and degraded extracellular matrix. Molecular analysis showed activation of antioxidant defenses, with superoxide dismutase (SOD) and catalase (CAT) activities significantly elevated (p < 0.05), while peroxidase (POD) activity declined (p < 0.05). Levels of malondialdehyde (MDA) and glutathione (GSH) also decreased. In detoxification responses, carboxylesterase (CarE) activity was reduced, whereas cytochrome P450 (P450) and glutathione S-transferase (GST) activities increased (p < 0.05). Transcriptome sequencing revealed 1,418 differentially expressed genes (DEGs), with notable upregulation of key detoxification genes (p < 0.05), including six cytochrome P450s (CYPs), five uridine diphosphate-glycosyltransferases (UGTs), three glutathione S-transferases (GSTs), and six ATP-binding cassette transporters (ABCs). KEGG enrichment analysis highlighted the involvement of these DEGs in drug metabolism, glutathione metabolism, and ABC transporter pathways (p < 0.05). Functional validation showed that knocking down Cap ‘n’ Collar C (CncC) significantly suppressed key detoxification genes (CYP18A1, CYP332A1, GSTd3, GSTt1, UGT33D8; p < 0.05). qRT-PCR and Western blot analyses confirmed that the Caspase-3 pathway mediates Pb-induced apoptosis, with increased cleaved Caspase-3 and Caspase-4 levels following CncC silencing. Overall, our findings elucidate the mechanisms of Pb toxicity in silkworms and identify CncC as a critical regulator of detoxification and defense against heavy metal stress in lepidopteran insects. Full article

(This article belongs to the Special Issue Insect Transcriptomics)

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13 pages, 1419 KiB

Open AccessArticle

Acetamiprid-Induced Toxicity Thresholds and Population Sensitivity in Trichogramma dendrolimi: Implications for Pesticide Risk Assessment

by Yan Zhang, Jiameng Ren and Shenhang Cheng

Insects 2025, 16(7), 698; https://doi.org/10.3390/insects16070698 - 7 Jul 2025

Trichogramma dendrolimi, a key egg parasitoid for lepidopteran pest control, faces potential risks from neonicotinoid insecticides like acetamiprid used in integrated pest management (IPM). This study evaluated acetamiprid’s acute and sublethal toxicity to T. dendrolimi and assessed population-level risks via species sensitivity [...] Read more.

Trichogramma dendrolimi, a key egg parasitoid for lepidopteran pest control, faces potential risks from neonicotinoid insecticides like acetamiprid used in integrated pest management (IPM). This study evaluated acetamiprid’s acute and sublethal toxicity to T. dendrolimi and assessed population-level risks via species sensitivity distribution (SSD). Acute toxicity assays using glass-vial residues revealed a 24 h LC₅₀ of 0.12 mg a.i. L⁻¹ for adults, three orders of magnitude below the maximum field rate (100 mg a.i. L⁻¹). Sublethal exposure (1/2–1/100 LC₅₀) significantly reduced parasitism and emergence rates (NOEC = 2.3 μg a.i. L⁻¹) but did not affect offspring survival. Acetamiprid also shortened offspring development at 11.5–57.5 μg a.i. L⁻¹. SSD analysis identified T. dendrolimi as the most sensitive parasitoid to acetamiprid (HC₅/HC₅₀) = 0.11/5.88 mg a.i. L⁻¹), with field rates (30–100 mg a.i. L⁻¹) indicating a potentially affected fraction (PAF) of 76.8–97.9%. These findings underscore the need to integrate sublethal effects into pesticide regulations to conserve parasitoid-mediated ecosystem services. Full article

(This article belongs to the Section Insect Pest and Vector Management)

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16 pages, 1124 KiB

Open AccessArticle

Development and Population Growth Rates of Sitophilus zeamais (Coleoptera: Curculionidae) Exposed to a Sublethal Concentration of Essential Oil of Piper hispidinervum

by Lucas Martins Lopes, Lêda Rita D’Antonino Faroni, Gutierres Nelson Silva, Douglas Rafael e Silva Barbosa, Marcela Silva Carvalho, Herus Pablo Firmino Martins, Thaís Rodrigues dos Santos, Igor da Silva Dias and Adalberto Hipólito de Sousa

Insects 2025, 16(7), 697; https://doi.org/10.3390/insects16070697 - 6 Jul 2025

Essential oils have emerged as promising alternatives for pest insect control. However, sublethal effects on insect reproduction and development are rarely explored, despite their relevance to integrated pest management (IPM). This study evaluated the sublethal effects of Piper hispidivervum C. DC. essential oil [...] Read more.

Essential oils have emerged as promising alternatives for pest insect control. However, sublethal effects on insect reproduction and development are rarely explored, despite their relevance to integrated pest management (IPM). This study evaluated the sublethal effects of Piper hispidivervum C. DC. essential oil (EOPH) on the development and population growth of four populations of Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae), as well as the persistence of safrole residue in treated corn grains. Population development rates were determined using emergence curves and total emerged adults, while population growth was assessed by counting live insects in the feeding substrate at different storage intervals. Safrole residue persistence was analyzed using solid-phase microextraction in headspace mode (SPME-HS). Sublethal exposure to EOPH significantly reduced the development rate, total emergence, and growth in three of the four populations. The population from Crixás, GO, showed no significant reduction, with a population curve overlapping the control. The lethal dose was reduced by 98.20%, indicating low persistence and potential food safety. The EOPH exhibited sublethal effects on S. zeamais populations, reducing both development rates and population growth. This reduction varied among the populations studied. Further research is encouraged to explore its effects on different insect populations and under broader environmental conditions. Full article

(This article belongs to the Special Issue Integrated Pest Management in Stored Products)

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16 pages, 1292 KiB

Open AccessArticle

Compartmentalization of Free Fatty Acids in Blood-Feeding Tabanus bovinus Females

by Mikołaj Drozdowski and Mieczysława Irena Boguś

Insects 2025, 16(7), 696; https://doi.org/10.3390/insects16070696 - 6 Jul 2025

Lipids play vital roles in insect physiology, functioning as energy reserves, membrane constituents, and cuticular protectants. However, few studies have examined the anatomical distribution of lipids in blood-feeding Diptera and compared the compositions of the cuticular and internal compartments. This study analyzes the [...] Read more.

Lipids play vital roles in insect physiology, functioning as energy reserves, membrane constituents, and cuticular protectants. However, few studies have examined the anatomical distribution of lipids in blood-feeding Diptera and compared the compositions of the cuticular and internal compartments. This study analyzes the qualitative and quantitative profiles of free fatty acids (FFAs) in the female Tabanus bovinus, a hematophagous horsefly species, across different anatomical regions, including the head, wings, legs, thorax, and abdomen. The surface and internal lipid fractions were isolated using petroleum ether/dichloromethane extraction followed by sonication. GC-MS revealed the presence of 21 FFAs, including 16 saturated (C7:0, C8:0, C9:0, C10:0, C11:0, C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0, C19:0, C20:0, C22:0, C24:0) and five unsaturated (C16:1, C18:2, C18:1, C20:5, C20:4). The head and wings showed the highest concentrations of cuticular FFAs. At the same time, internal lipid stores were most prominent in the thorax and abdomen (but four times lower than in the head cuticle), reflecting their role in energy storage and reproduction. All cuticular and internal extracts were dominated by C16:0, C18:0, and C18:1. Notably, several FFAs were undetected in specific compartments: C10:0 from inside the head, C11:0 and C13:0 from inside all examined body parts, C19:0 was absent from inside the head, wings and legs, while C20:5 and C20:4 were absent from both the cuticular and internal lipid pools of the wings. Interestingly, our analysis of the cuticle on the thorax and abdomen together revealed that both C13:0 and C19:0 were present only on the dorsal side, i.e., absent from the ventral side. These absences suggest a selective lipid metabolism tailored to the functional and ecological demands of T. bovinus females. Our findings suggest that the absence of specific compounds from individual body parts may serve as an indicator of physiological specialization. This work provides new insights into lipid compartmentalization in Tabanidae and offers a framework for future comparative and ecological lipidomics studies in insects. Full article

(This article belongs to the Section Insect Physiology, Reproduction and Development)

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13 pages, 594 KiB

Open AccessArticle

Predation Risk Effects of Harmonia axyridis on the Development and Fecundity of Periphyllus koelreuteriae

by Haibo Yang, Jiaoyi Du, Lei Wang, Pinhong Zhu, Dingxu Li, Jianrong Huang and Zhenjie Hu

Insects 2025, 16(7), 695; https://doi.org/10.3390/insects16070695 - 6 Jul 2025

In the scenario that requires the biological control of pests using predatory enemies, predators not only prey on pests directly but also can affect the population fitness of pests through indirect non-consumptive effects (predation risk effects). However, the impact of predation risk effects [...] Read more.

In the scenario that requires the biological control of pests using predatory enemies, predators not only prey on pests directly but also can affect the population fitness of pests through indirect non-consumptive effects (predation risk effects). However, the impact of predation risk effects varies depending on the mode of stress imposed by natural enemies and the state of the stressed pests. Herein, we exposed aphids (Periphyllus koelreuteriae) at different stages to various cues from the multicolored Asian lady beetle (Harmonia axyridis) to assess the effects of different predation risks on P. koelreuteriae development and reproduction. We found that the effect of predation risk on aphid developmental time was clearly stage-dependent. When 1st-instar nymphs were exposed to predator cues, their developmental time was prolonged only in the early stages (1st–3rd instar nymphs). Similarly, when third-instar nymphs were stressed, only the current stage (third instar) showed a developmental delay, while fourth-instar nymphs and adult aphids were not significantly affected. Additionally, aphids at different stages perceive predation risk differently. Nymphs cannot recognize visual cues from predators, but can perceive odor cues. In contrast, adult aphids are sensitive to a variety of predator cues, including visual ones. Predation risk caused aphids to shorten their adult lifespan and reduce reproductive output, but it increased the proportion of diapause offspring. This study highlights the importance of considering how predation risk affects multiple life stages and physiological changes, which are important for gaining insights into the mechanisms of predator–prey interactions and for comprehensively assessing the ability of lady beetles to regulate aphid populations. Full article

(This article belongs to the Section Insect Physiology, Reproduction and Development)

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20 pages, 320 KiB

Open AccessReview

The Contribution of Molecular Biology to Forensic Entomology

by Carmen Scieuzo, Roberta Rinaldi, Federica De Stefano, Aldo Di Fazio and Patrizia Falabella

Insects 2025, 16(7), 694; https://doi.org/10.3390/insects16070694 - 5 Jul 2025

This review presents an in-depth analysis of the synergistic role of molecular biology in advancing forensic entomology. The study discusses how insects associated with decomposing bodies provide critical data for estimating the post-mortem interval (PMI), and how molecular techniques improve species identification and [...] Read more.

This review presents an in-depth analysis of the synergistic role of molecular biology in advancing forensic entomology. The study discusses how insects associated with decomposing bodies provide critical data for estimating the post-mortem interval (PMI), and how molecular techniques improve species identification and trace analysis. The manuscript examines DNA-based methods such as RAPD, RFLP, and mitochondrial sequencing, along with innovative applications like gene expression profiling and entomotoxicology analysis. Additionally, it presents real case studies illustrating how molecular data from insects can be used not only to estimate PMI but also to identify victims or suspects through human DNA retrieved from insect tissues. These advances confirm the fundamental role of molecular biology in strengthening the reliability and applicability of forensic entomology in legal contexts. Full article

(This article belongs to the Special Issue Women’s Special Issue Series: Insects)

12 pages, 243 KiB

Open AccessArticle

Acaricidal Efficacy of Diatomaceous Earths on Different Life Stages of Acarus siro L. and Tyrophagus putrescentiae (Schrank)

by Maria C. Boukouvala, Constantin S. Filintas and Nickolas G. Kavallieratos

Insects 2025, 16(7), 693; https://doi.org/10.3390/insects16070693 - 4 Jul 2025

Acarus siro L. and Tyrophagus putrescentiae (Schrank) (Sarcoptiformes: Acaridae) are cosmopolitan mite species in food storage and processing environments, infesting a wide variety of commodities. In the current study, the diatomaceous earths (DEs) InsectoSec and Fossil Shield were evaluated for wheat protection against [...] Read more.

Acarus siro L. and Tyrophagus putrescentiae (Schrank) (Sarcoptiformes: Acaridae) are cosmopolitan mite species in food storage and processing environments, infesting a wide variety of commodities. In the current study, the diatomaceous earths (DEs) InsectoSec and Fossil Shield were evaluated for wheat protection against adults, larvae, and nymphs of A. siro and T. putrescentiae. Both DEs were examined at 200 and 500 ppm at 1, 2, and 5 days post-exposure. The efficacy of both formulations against A. siro and T. putrescentiae life stages depended on dose and exposure. Mortality of A. siro reached 100% in larvae, 99.3% in nymphs, and 95.6% in adults by day 5 at 500 ppm of InsectoSec. Similarly, Fossil Shield achieved almost complete larval mortality (99.3%) of A. siro at 500 ppm. For T. putrescentiae, 100% mortality was observed for larvae at both doses of InsectoSec and for adults or nymphs at 500 ppm by the fifth day. Fossil Shield caused a similar mortality to larvae, reaching 97.0% and 100%, at 200 and 500 ppm, respectively, after 5 days. Our findings indicate that InsectoSec and Fossil Shield can be used as sustainable management tools against A. siro and T. putrescentiae. Full article

(This article belongs to the Special Issue Advances in the Effects of Insecticides on Pests)

20 pages, 3332 KiB

Open AccessArticle

Impact of Salmonella enteritidis Infection and Mechanical Stress on Antimicrobial Peptide Expression in Hermetia illucens

by Davide Santori, Anna Maria Fausto, Alessio Gelli, Anna Rita Pifferi, Samuele Dottarelli, Sofia Cucci, Francesca Di Donato, Goffredo Grifoni and Erminia Sezzi

Insects 2025, 16(7), 692; https://doi.org/10.3390/insects16070692 - 4 Jul 2025

Hermetia illucens, the black soldier fly, is a common and widespread fly of the family Stratiomyidae. Its ability to grow on contaminated substrates suggests the production of antimicrobial peptides that enable its survival. This study aimed to verify the impact of [...] Read more.

Hermetia illucens, the black soldier fly, is a common and widespread fly of the family Stratiomyidae. Its ability to grow on contaminated substrates suggests the production of antimicrobial peptides that enable its survival. This study aimed to verify the impact of direct and indirect infection with Salmonella enteritidis on the expression of defensins and cecropins in Hermetia illucens larvae. In addition to an infection with a microorganism, it was interesting to verify if the expression of peptides and the relative action of hemolymph changed in larvae subjected to mechanical stress by abdominal puncture. The peptide fraction of the hemolymph of infected larvae was tested using antibiogram and minimum inhibitory concentration tests against Salmonella enteritidis and Salmonella typhimurium. Both molecular and microbiological tests were carried out at three different time points, on larvae not subjected to any treatment (T-0), four hours after treatment (T-1), and 24 h after treatment (T-2). The results of the microbiological tests showed the antimicrobial action of the peptide fraction of the hemolymph against both S. typhimurium and S. enteritidis; for the latter one, the action was more marked. Interesting results were also found for larvae subjected only to mechanical stress by puncture. Molecular tests on the expression of defensins and cecropins were in full agreement with those obtained in the microbiological tests, with expression more pronounced in larvae infected directly with Salmonella enteritidis. Temporal and condition-specific regulation of defensins and cecropins highlights the complexity of the immune response and suggests sophisticated mechanisms by which the host fine-tunes antimicrobial peptide expression to enhance pathogen defense while preventing excessive immune activation. Full article

(This article belongs to the Section Insect Behavior and Pathology)

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20 pages, 2408 KiB

Open AccessArticle

Evaluation of Mating Disruption for Suppression of Plodia interpunctella Populations in Retail Stores

by James F. Campbell, James Miller, James Petersen and Bill Lingren

Insects 2025, 16(7), 691; https://doi.org/10.3390/insects16070691 - 3 Jul 2025

Mating disruption is a commercially available management tactic for pyralid moths, which are pests of stored products. However, evaluations of efficacy have had limited replication, which limits the ability to draw conclusions about its effectiveness or the impact of different variables on its [...] Read more.

Mating disruption is a commercially available management tactic for pyralid moths, which are pests of stored products. However, evaluations of efficacy have had limited replication, which limits the ability to draw conclusions about its effectiveness or the impact of different variables on its efficacy. We evaluated the mating disruption of Plodia interpunctella in 33 retail pet supply stores (6415 to 17,384 m³) and the impact of factors such as insect density and application rate on efficacy. Prior to starting MD, the average capture of P. interpunctella was 40.2 ± 3.6 moths/trap/month. Immediately after starting treatment, there was a sharp drop in captures (67.8 ± 4.8%) and then a more gradual overall downward. Overall, under mating disruption, the average reduction was 85.0 ± 3.0%. Geographic location, initial moth density, and pheromone application rate did not significantly impact efficacy. Analysis of the relationships between moth captures and mating disruption dispenser density indicated that competitive mechanisms were the primary mechanisms involved. This was the largest replicated assessment of MD for the management of a post-harvest pest and provides valuable foundational and applied insights into the process. Our results show that a standardized MD program can provide pest suppression in retail stores, but it takes time to be fully effective. Finally, identifying the primary mechanism for efficacy provides important information needed for further refinement of MD programs. Full article

(This article belongs to the Special Issue Chemical and Non-Chemical Control in Stored Product Protection: Modern Methods for Old Problems)

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24 pages, 1449 KiB

Open AccessReview

Heortia vitessoides Infests Aquilaria sinensis: A Systematic Review of Climate Drivers, Management Strategies, and Molecular Mechanisms

by Zongyu Yin, Yingying Chen, Huanrong Xue, Xiaofei Li, Baocai Li, Jiaming Liang, Yongjin Zhu, Keyu Long, Jinming Yang, Jiao Pang, Kaixiang Li and Shaoming Ye

Insects 2025, 16(7), 690; https://doi.org/10.3390/insects16070690 - 2 Jul 2025

Heortia vitessoides Moore (Lepidoptera: Pyralidae), the dominant outbreak defoliator of Aquilaria sinensis (Myrtales: Thymelaeaceae, the agarwood-producing tree), poses a severe threat to the sustainable development of the agarwood industry. Current research has preliminarily revealed its biological traits and gene functions. However, significant gaps [...] Read more.

Heortia vitessoides Moore (Lepidoptera: Pyralidae), the dominant outbreak defoliator of Aquilaria sinensis (Myrtales: Thymelaeaceae, the agarwood-producing tree), poses a severe threat to the sustainable development of the agarwood industry. Current research has preliminarily revealed its biological traits and gene functions. However, significant gaps persist in integrating climate adaptation mechanisms, control technologies, and host interaction networks across disciplines. This review systematically synthesizes the multidimensional mechanisms underlying H. vitessoides outbreaks through the logical framework of “Fundamental Biology of Outbreaks—Environmental Drivers—Control Strategies—Molecular Regulation—Host Defense.” First, we integrate the biological characteristics of H. vitessoides with its climatic response patterns, elucidating the ecological pathways through which temperature and humidity drive population outbreaks by regulating development duration and host resource availability. Subsequently, we assess the efficacy and limitations of existing control techniques (e.g., pheromone trapping, Beauveria bassiana application), highlighting the critical bottleneck of insufficient mechanistic understanding at the molecular level. Building on this, we delve into the molecular adaptation mechanisms of H. vitessoides. Specifically, detoxification genes (e.g., HvGSTs1) and temperature stress-responsive genes (e.g., HvCAT, HvGP) synergistically enhance stress tolerance, while chemosensory genes mediate mating and host location behaviors. Concurrently, we reveal the host defense strategy of A. sinensis, involving activation of secondary metabolite defenses via the jasmonic acid signaling pathway and emission of volatile organic compounds that attract natural enemies—an “induced resistance–natural enemy collaboration” mechanism. Finally, we propose future research directions: deep integration of gene editing to validate key targets, multi-omics analysis to decipher the host–pest–natural enemy interaction network, and development of climate–gene–population dynamics models. These approaches aim to achieve precision control by bridging molecular mechanisms with environmental regulation. This review not only provides innovative pathways for managing H. vitessoides but also establishes a paradigm for cross-scale research on pests affecting high-value economic forests. Full article

(This article belongs to the Section Insect Pest and Vector Management)

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5 pages, 619 KiB

Open AccessBrief Report

A “Sconce” Trap for Sampling Egg Masses of Spotted Lanternfly, Lycorma delicatula

by Sarah M. Devine, Everett G. Booth, Miriam F. Cooperband, Emily K. L. Franzen, Phillip A. Lewis, Kelly M. Murman and Joseph A. Francese

Insects 2025, 16(7), 689; https://doi.org/10.3390/insects16070689 - 1 Jul 2025

Survey and detection of the spotted lanternfly, Lycorma delicatula (White), rely either on traps that exploit the insect’s behavior as it navigates its environment, or on visual surveys of either its mobile life stages or egg masses. A recently described egg mass trap, [...] Read more.

Survey and detection of the spotted lanternfly, Lycorma delicatula (White), rely either on traps that exploit the insect’s behavior as it navigates its environment, or on visual surveys of either its mobile life stages or egg masses. A recently described egg mass trap, coined the “lampshade” trap, can assist with early detection in newly infested areas, provide egg masses for researchers, and potentially facilitate spotted lanternfly population reduction by removal of egg masses from the environment. Here, we describe a modified lampshade trap, the sconce trap, that uses less material, can be pre-cut prior to deployment, and can be deployed by one person, representing potential cost, labor, and time savings. Both traps were comparable at detecting populations of spotted lanternflies, and while females deposited more eggs on the larger lampshade traps, they deposited more eggs on sconce traps as a function of trap area. Full article

(This article belongs to the Section Insect Pest and Vector Management)

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13 pages, 1285 KiB

Open AccessArticle

Symbiont-Targeted Control of Halyomorpha halys Does Not Affect Local Insect Diversity in a Hazelnut Orchard

by Sofia Victoria Prieto, Matteo Dho, Bianca Orrù, Elena Gonella and Alberto Alma

Insects 2025, 16(7), 688; https://doi.org/10.3390/insects16070688 - 30 Jun 2025

Harmless crop-associated insect communities are a fundamental part of the agroecosystem. Their potential as a reservoir of natural enemies of pests has encouraged their conservation through the development of low-impact pest management programs. The brown marmorated stink bug, Halyomorpha halys, represents a serious [...] Read more.

Harmless crop-associated insect communities are a fundamental part of the agroecosystem. Their potential as a reservoir of natural enemies of pests has encouraged their conservation through the development of low-impact pest management programs. The brown marmorated stink bug, Halyomorpha halys, represents a serious threat to Italian hazelnut production. Laboratory and field experiments confirmed the susceptibility of this pest to the disruption of the obligated symbiotic interaction with gut bacteria, paving the way for the development of the symbiont-targeted control strategy. Here we present the results of a three-year field assessment of symbiont-targeted control in a hazelnut orchard in northwestern Italy. The use of a biocomplex to disrupt symbiont acquisition by H. halys nymphs was compared to the use of lambda-cyhalothrin insecticide. The effects on the local entomofauna were assessed, as were the trend of H. halys population and the damage caused by stink bugs to harvested hazelnuts. The insecticide consistently reduced the insect diversity in the field, while the anti-symbiont biocomplex had no effect. However, the control of the H. halys population and the stink bug-induced damage to hazelnuts varied over the years in the field plot submitted to the symbiont-targeted approach. Our results indicate that the symbiont-targeted control does not interfere with local insect communities. Key aspects for improving the effectiveness of this tactic are discussed. Full article

(This article belongs to the Special Issue Surveillance and Management of Invasive Insects)

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13 pages, 3755 KiB

Open AccessArticle

Diversity of Termites Used in Poultry Feed in Burkina Faso

by Aïchatou Nadia Christelle Dao, Fernand Sankara, Mouhamadou Moustapha Ndiaye, Abdoulaye Baïla Ndiaye, Salimata Pousga, Irénée Somda and Marc Kenis

Insects 2025, 16(7), 687; https://doi.org/10.3390/insects16070687 - 30 Jun 2025

The aim of the study was to assess the diversity of termites used in poultry feed in Burkina Faso. Termite samples were collected in eight of the thirteen regions of the country by poultry farmers, then conserved in 70° alcohol. The criteria used [...] Read more.

The aim of the study was to assess the diversity of termites used in poultry feed in Burkina Faso. Termite samples were collected in eight of the thirteen regions of the country by poultry farmers, then conserved in 70° alcohol. The criteria used by poultry farmers for identifying the termite were also characterised and discussed with farmers in a village where the use of termites as poultry feed is well developed. Morphological identifications were carried out in the laboratory. Twenty species were identified in two families, six subfamilies, and thirteen genera. In the Heterotermitidae family, a single species belonging to the Coptotermitinae subfamily was identified. The rest belonged to the family Termitidae and the subfamilies Amitermitinae, Microcerotermitinae, Macrotermitinae, Nasutitermitinae, and Cubitermitinae. Three species, Microcerotermes fuscotibialis, Megagnathotermes notandus, and Isognathotermes fungifaber, were found for the first time in Burkina Faso. The largest number of species (eleven) was collected in the Cascades region. Poultry farmers are able to recognise eight genera of termites by the shape, size, and colour of the termites; by the termite mounds; and often by the location of the nest. These results may facilitate the promotion of the use of termites as poultry feed in Burkina Faso and West Africa. Full article

(This article belongs to the Section Role of Insects in Human Society)

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16 pages, 2812 KiB

Open AccessArticle

Industrial-Scale Bioconversion of Three-Phase Residue by Musca domestica Larvae: Dynamics of Gut Microbiota and Their Ecological Driver

by Wenna Long, Junran Pang, Wantao Yan and Nan Hu

Insects 2025, 16(7), 686; https://doi.org/10.3390/insects16070686 - 30 Jun 2025

The escalating global population coupled with rising living standards in developing nations has created dual challenges in sustainable food production and organic waste management [...] Full article

(This article belongs to the Section Insect Behavior and Pathology)

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27 pages, 15418 KiB

Open AccessArticle

Revision of Macrima Baly, 1878 (Coleoptera: Chrysomelidae: Galerucinae)

by Chuan Feng, Xingke Yang, Jan Bezděk and Siqin Ge

Insects 2025, 16(7), 685; https://doi.org/10.3390/insects16070685 - 30 Jun 2025

This study presents a taxonomic revision of the genus Macrima Baly, 1878 to advance the understanding of suprageneric, non-monophyletic “Monoleptites”. Through comprehensive morphological examinations of all type specimens, including detailed analyses of male and female genitalia and the structure of the cavitous anterior [...] Read more.

This study presents a taxonomic revision of the genus Macrima Baly, 1878 to advance the understanding of suprageneric, non-monophyletic “Monoleptites”. Through comprehensive morphological examinations of all type specimens, including detailed analyses of male and female genitalia and the structure of the cavitous anterior part of the head, we propose M. roseofulva Medvedev, 2011 and M. ferrugina Jiang, 1990 as new synonyms of M. aurantiaca (Laboissière, 1936), M. bifida Yang, 1992 a new synonym of M. cornuta (Laboissière, 1936), and M. yunnanensis (Laboissière, 1936) as a new synonym of M. pallida (Laboissière, 1936). In addition, M. costatipennis Jacoby, 1896 is transferred to Hoplosaenidea Laboissière, 1933 (comb. nov.). Color photos of habitus, body details, and genitalia are presented for all species. Full article

(This article belongs to the Special Issue Revival of a Prominent Taxonomy of Insects)

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16 pages, 4080 KiB

Open AccessArticle

Knocked-Out Bombyx mori Protein Disulfide Isomerase Decreases Silk Yields and Mechanical Properties by Affecting the Post-Translational Modification of Silk Proteins

by Shifeng Yang, Mengyao He, Xian Li, Huan Dong, Hexu Lei, Fangyu Wang, Hanxin Deng, Hongji Zhou, Siyu Chen, Yujuan Zhou, Zihan Meng, Ding Tu, He Wang, Qingyou Xia and Feng Wang

Insects 2025, 16(7), 684; https://doi.org/10.3390/insects16070684 - 30 Jun 2025

Silkworms synthesize and secrete silk to produce cocoons, which are excellent materials for textile and biomaterial manufacturing applications. However, the gene regulation associated with the post-translational modification of silk proteins remains unknown. In this study, we analyzed the physicochemical properties, evolutionary relationships, and [...] Read more.

Silkworms synthesize and secrete silk to produce cocoons, which are excellent materials for textile and biomaterial manufacturing applications. However, the gene regulation associated with the post-translational modification of silk proteins remains unknown. In this study, we analyzed the physicochemical properties, evolutionary relationships, and expression patterns of BmPDI in silkworms. Subsequently, we knocked out BmPDI (BmPDI-KO), resulting in significant phenotypes of BmPDI-KO silkworms with smaller silk glands and cocoons, weaker silk mechanical properties, and reduced disulfide bonds in silk-associated proteins. Transcription levels of silk protein-related genes and unfolded protein response signal pathway-related genes were significantly downregulated. In contrast, genes involved in the apoptosis pathway were significantly upregulated in BmPDI-KO silkworms. Knocking out BmPDI in silkworms affected the post-translational modifications of the silk proteins, thereby accumulating misfolded silk proteins and hindering their secretion into the extracellular cells. This further increased endoplasmic reticulum stress, activated the apoptotic pathway, accelerated silk gland cell apoptosis, and significantly reduced the silk yields and mechanical properties of BmPDI-KO silkworm. This study provides a potential exploration of BmPDI in the modification of silk yields and mechanical properties of Bombyx mori. Full article

(This article belongs to the Special Issue Recent Studies on Resource Insects)

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11 pages, 5750 KiB

Open AccessArticle

Management Diversification Increases Habitat Availability for Lepidoptera Papilionoidea in the Torretes Biological Station (Spain)

by Javier Quinto, Elena Espín and Eduardo Galante

Insects 2025, 16(7), 683; https://doi.org/10.3390/insects16070683 - 30 Jun 2025

Butterflies (Lepidoptera: Papilionoidea), considered bioindicators of habitat conservation status, are one of the groups used to assess the impact of habitat management and improvement efforts in the Biological Station of Torretes (Ibi, Alicante, Spain). This area also houses the Botanical Garden of the [...] Read more.

Butterflies (Lepidoptera: Papilionoidea), considered bioindicators of habitat conservation status, are one of the groups used to assess the impact of habitat management and improvement efforts in the Biological Station of Torretes (Ibi, Alicante, Spain). This area also houses the Botanical Garden of the University of Alicante. The study was focused on the period from autumn 2022 to late spring 2023, and data on butterfly diversity and abundance from the current study were compared with historical data. Our results highlight that open spaces and the availability of trophic resources (nutritional plants) were key factors influencing the richness and abundance of Papilionoidea. The program for the creation and maintenance of new habitats at the biological station has increased spatial heterogeneity and the availability of trophic resources, which has led to a significant increase in the richness and abundance of butterflies in a short period. To support the conservation of Mediterranean ecosystems, and entomofauna in general, the Papilionidae are proposed as model groups for designing conservation projects based on traditional habitat management. These projects should promote spatial heterogeneity, include programs to enrich plant diversity, and be complemented by environmental education and outreach initiatives. Full article

(This article belongs to the Special Issue Advances on Conservation of Biodiversity, Monitoring Programs and Trend Assessment)

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21 pages, 13994 KiB

Open AccessArticle

Fine Structure and Optical Features of the Compound Eyes of Adult Female Ceratosolen gravelyi (Hymenoptera: Agaonidae)

by Hua Xie, Yan Shi, Shouxian Zhang, Yonghui Zhu, Subo Shao, Yuan Zhang, Pei Yang and Zongbo Li

Insects 2025, 16(7), 682; https://doi.org/10.3390/insects16070682 - 30 Jun 2025

Pollinating fig wasps (Agaonidae) engage in an obligate mutualism with Ficus species, which is mediated by host-specific chemical cues. However, the role of visual perception in host recognition remains poorly understood, particularly because of a lack of structural studies of their compound eyes. [...] Read more.

Pollinating fig wasps (Agaonidae) engage in an obligate mutualism with Ficus species, which is mediated by host-specific chemical cues. However, the role of visual perception in host recognition remains poorly understood, particularly because of a lack of structural studies of their compound eyes. We investigated the ocular morphology of female Ceratosolen gravelyi (exclusive pollinator of F. semicordata) using scanning/transmission electron microscopy. The oval apposition eyes contain 228–263 ommatidia, which are asymmetrically distributed between the left and right eyes. Each ommatidium comprises a biconvex corneal lens overlying a tetrapartite eucone crystalline cone; proximal cone cells reveal an interlaced labyrinth. Pigment cells encapsulate each ommatidium, and numerous pigment granules and mitochondria are present in both pigment and retinular cells. Nine retinular cells comprise a unit, with eight photoreceptors (R1–R8) forming the rhabdom from the cone base to the basal matrix; a ninth cell replaces R8 in the apical third of the rhabdom. Optical metrics, including F-number (1.1°), acceptance angle (10.0°), and ommatidial sensitivity (0.26 µm²/sr), indicate diurnal activity in bright environments. These adaptations suggest that their eyes are critical for processing visual cues during host interactions, which advances our understanding of multimodal sensory integration in fig–wasp mutualism. Full article

(This article belongs to the Section Insect Physiology, Reproduction and Development)

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