- Article
FLIM-Based Intracellular and Extracellular pH Measurements Using Genetically Encoded pH Sensor
- Alexander S. Goryashchenko,
- Alexey A. Pakhomov,
- Anastasia V. Ryabova,
- Igor D. Romanishkin,
- Eugene G. Maksimov,
- Alexander N. Orsa,
- Oxana V. Serova,
- Andrey A. Mozhaev,
- Margarita A. Maksimova and
- Igor E. Deyev
- + 2 authors
The determination of pH in live cells and tissues is of high importance in physiology and cell biology. In this report, we outline the process of the creation of SypHerExtra, a genetically encoded fluorescent sensor that is capable of measuring extra...