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  • Article
  • Open Access
6 Citations
3,759 Views
15 Pages

Microbial Communities of Flor Velums and the Genetic Stability of Flor Yeasts Used for a Long Time for the Industrial Production of Sherry-like Wines

  • Andrey V. Mardanov,
  • Eugeny V. Gruzdev,
  • Alexey V. Beletsky,
  • Elena V. Ivanova,
  • Maksim Yu. Shalamitskiy,
  • Tatiana N. Tanashchuk and
  • Nikolai V. Ravin

Flor yeast strains represent a specialized group of Saccharomyces cerevisiae yeasts used for the production of sherry-like wines by biological wine aging. We sequenced the genome of the industrial flor yeast strain I-329 from a collection of microorg...

(This article belongs to the Special Issue Yeast - Fermentation)
  • Review
  • Open Access
3 Citations
1,853 Views
26 Pages

8 December 2025

The Criaderas and Solera system represents one of the most sophisticated aging methodologies in winemaking, producing distinctive Sherry wines through dynamic blending and biological aging processes. This traditional Spanish system, combined with the...

(This article belongs to the Special Issue Feature Reviews on Food Microbiology)
  • Article
  • Open Access
12 Citations
4,034 Views
15 Pages

Metabolic Changes by Wine Flor-Yeasts with Gluconic Acid as the Sole Carbon Source

  • Minami Ogawa,
  • Jaime Moreno-García,
  • Lucy C. M. Joseph,
  • Juan C. Mauricio,
  • Juan Moreno and
  • Teresa García-Martínez

Gluconic acid consumption under controlled conditions by a Saccharomyces cerevisiae flor yeast was studied in artificial media. Gluconic acid was the sole carbon source and the compounds derived from this metabolism were tracked by endo-metabolomic a...

(This article belongs to the Section Microbiology and Ecological Metabolomics)
  • Review
  • Open Access
16 Citations
8,725 Views
21 Pages

The manufacturing of sherry wines is a unique, carefully regulated process, from harvesting to quality control of the finished product, involving dynamic biological aging in a “criadera-solera” system or some other techniques. Specialized...

(This article belongs to the Special Issue Wine Microbiology)
  • Article
  • Open Access
1 Citations
1,360 Views
19 Pages

Metabolic Characterization of Two Flor Yeasts During Second Fermentation in the Bottle for Sparkling Wine Production

  • Juan Carlos García-García,
  • María Trinidad Alcalá-Jiménez,
  • Juan Carlos Mauricio,
  • Cristina Campos-Vázquez,
  • Inés M. Santos-Dueñas,
  • Juan Moreno and
  • Teresa García-Martínez

28 October 2025

The global sparkling wine market continues to grow steadily, reaching approximately 24 million hectoliters in 2023, with an annual increase of around 4% despite a general decline in overall alcoholic beverage consumption. This growth highlights the i...

(This article belongs to the Special Issue Microbial Fermentation: Advances in Synthetic Biology, Metabolic Engineering, and Biomanufacturing)
  • Article
  • Open Access
8 Citations
3,563 Views
13 Pages

Comparative Study of the Proteins Involved in the Fermentation-Derived Compounds in Two Strains of Saccharomyces cerevisiae during Sparkling Wine Second Fermentation

  • María del Carmen González-Jiménez,
  • Teresa García-Martínez,
  • Juan Carlos Mauricio,
  • Irene Sánchez-León,
  • Anna Puig-Pujol,
  • Juan Moreno and
  • Jaime Moreno-García

Sparkling wine is a distinctive wine. Saccharomyces cerevisiae flor yeasts is innovative and ideal for the sparkling wine industry due to the yeasts’ resistance to high ethanol concentrations, surface adhesion properties that ease wine clarific...

(This article belongs to the Special Issue Microbial Secondary Metabolites and Biotechnology)
  • Article
  • Open Access
2 Citations
3,613 Views
14 Pages

Stress Resistance and Adhesive Properties of Commercial Flor and Wine Strains, and Environmental Isolates of Saccharomyces cerevisiae

  • Michail A. Eldarov,
  • Daria A. Avdanina,
  • Elena Ivanova,
  • Maksim Y. Shalamitskiy,
  • Tatiana N. Tanashchuk,
  • Tatiana Vybornaya,
  • Nikolai V. Ravin,
  • Svetlana A. Kishkovskaya and
  • Andrey V. Mardanov

Flor strains of Saccharomyces cerevisiae represent a special group of yeasts used for producing biologically aged wines. We analyzed the collection of commercial wine and flor yeast strains, as well as environmental strains isolated from the surface...

(This article belongs to the Section Fermentation for Food and Beverages)
  • Article
  • Open Access
8 Citations
8,312 Views
10 Pages

A Microtiter Plate Assay as a Reliable Method to Assure the Identification and Classification of the Veil-Forming Yeasts during Sherry Wines Ageing

  • Marina Ruíz-Muñoz,
  • Maria Del Carmen Bernal-Grande,
  • Gustavo Cordero-Bueso,
  • Mónica González,
  • David Hughes-Herrera and
  • Jesús Manuel Cantoral

Yeasts involved in veil formation during biological ageing of Sherry wines are mainly Saccharomyces cerevisiae, and they have traditionally been divided into four races or varieties: beticus, cheresiensis, montuliensis and rouxii. Recent progress in...

(This article belongs to the Special Issue Yeast Biotechnology 2.0)
  • Article
  • Open Access
22 Citations
4,028 Views
18 Pages

Differential Analysis of Proteins Involved in Ester Metabolism in two Saccharomyces cerevisiae Strains during the Second Fermentation in Sparkling Wine Elaboration

  • Maria del Carmen González-Jiménez,
  • Jaime Moreno-García,
  • Teresa García-Martínez,
  • Juan José Moreno,
  • Anna Puig-Pujol,
  • Fina Capdevilla and
  • Juan Carlos Mauricio

The aromatic metabolites derived from yeast metabolism determine the characteristics of aroma and taste in wines, so they are considered of great industrial interest. Volatile esters represent the most important group and therefore, their presence is...

(This article belongs to the Special Issue Yeast in Winemaking)
  • Review
  • Open Access
30 Citations
10,053 Views
11 Pages

The Microbial Diversity of Sherry Wines

  • Gustavo Cordero-Bueso,
  • Marina Ruiz-Muñoz,
  • Mónica González-Moreno,
  • Salvador Chirino,
  • María Del Carmen Bernal-Grande and
  • Jesús Manuel Cantoral

The principal role of wine yeast is to transform efficiently the grape-berries’ sugars to ethanol, carbon dioxide, and other metabolites, without the production of off-flavors. Wine yeast strains are able to ferment musts, while other commercial or l...

(This article belongs to the Special Issue Microbiota of Fermented Beverages)
  • Article
  • Open Access
24 Citations
7,294 Views
18 Pages

Differential Proteome Analysis of a Flor Yeast Strain under Biofilm Formation

  • Jaime Moreno-García,
  • Juan Carlos Mauricio,
  • Juan Moreno and
  • Teresa García-Martínez

Several Saccharomyces cerevisiae strains (flor yeasts) form a biofilm (flor velum) on the surface of Sherry wines after fermentation, when glucose is depleted. This flor velum is fundamental to biological aging of these particular wines. In this stud...

(This article belongs to the Special Issue Biofilm Formation)
  • Article
  • Open Access
22 Citations
4,369 Views
14 Pages

Use of Multiflora Bee Pollen as a Flor Velum Yeast Growth Activator in Biological Aging Wines

  • Pau Sancho-Galán,
  • Antonio Amores-Arrocha,
  • Ana Jiménez-Cantizano and
  • Víctor Palacios

Flor velum yeast growth activators during biological aging are currently unknown. In this sense, this research focuses on the use of bee pollen as a flor velum activator. Bee pollen influence on viable yeast development, surface hydrophobicity, and y...

(This article belongs to the Special Issue Chemical/Instrumental Approaches to the Evaluation of Wine Chemistry)
  • Article
  • Open Access
3 Citations
2,454 Views
20 Pages

Comparative Proteomics of Two Flor Yeasts in Sparkling Wine Fermentation: First Approach

  • Juan C. García-García,
  • Teresa García-Martínez,
  • Juan J. Román-Camacho,
  • Juan Moreno and
  • Juan C. Mauricio

16 January 2025

The traditional method is considered the highest-quality sparkling wine making technique. Its main characteristic is that the entire sparkling transformation takes place in the bottle, producing complex, refined wines with fine, persistent bubbles. C...

(This article belongs to the Special Issue Innovative Microbiological Technologies and Applications in Fermented Food Products for the International Conference on Traditionally Fermented Foods in the World—2024 Acetic Acid Bacteria and Monascus (2024-AAB&M))
  • Article
  • Open Access
3 Citations
1,760 Views
18 Pages

Nitrogen Metabolism in Two Flor Yeast Strains at Mid-Second Bottle Fermentation in Sparkling Wine Production

  • Juan Carlos García-García,
  • Miguel E. G-García,
  • Juan Carbonero-Pacheco,
  • Inés M. Santos-Dueñas,
  • Juan Carlos Mauricio,
  • María Trinidad Alcalá-Jiménez,
  • Juan Moreno and
  • Teresa García-Martínez

16 May 2025

This study investigates nitrogen metabolism during the middle of the second fermentation in stopped bottles of sparkling wine, focusing on two flor Saccharomyces cerevisiae yeast strains (G1 and N62) isolated from the velum of biologically aged wine....

(This article belongs to the Special Issue Wine Technology and Sensory Analysis)
  • Article
  • Open Access
6 Citations
3,803 Views
15 Pages

A Differential Proteomic Approach to Characterize the Cell Wall Adaptive Response to CO2 Overpressure during Sparkling Wine-Making Process

  • Juan Antonio Porras-Agüera,
  • Juan Carlos Mauricio,
  • Jaime Moreno-García,
  • Juan Moreno and
  • Teresa García-Martínez

In this study, a first proteomic approach was carried out to characterize the adaptive response of cell wall-related proteins to endogenous CO2 overpressure, which is typical of second fermentation conditions, in two wine Saccharomyces cerevisiae str...

(This article belongs to the Special Issue Microbial Cell Wall)
  • Article
  • Open Access
14 Citations
4,130 Views
20 Pages

First Proteomic Approach to Identify Cell Death Biomarkers in Wine Yeasts during Sparkling Wine Production

  • Juan Antonio Porras-Agüera,
  • Jaime Moreno-García,
  • Juan Carlos Mauricio,
  • Juan Moreno and
  • Teresa García-Martínez

Apoptosis and later autolysis are biological processes which take place in Saccharomyces cerevisiae during industrial fermentation processes, which involve costly and time-consuming aging periods. Therefore, the identification of potential cell death...

(This article belongs to the Special Issue Yeast in Winemaking)
  • Article
  • Open Access
12 Citations
3,899 Views
15 Pages

Autophagic Proteome in Two Saccharomyces cerevisiae Strains during Second Fermentation for Sparkling Wine Elaboration

  • Juan Antonio Porras-Agüera,
  • Jaime Moreno-García,
  • María del Carmen González-Jiménez,
  • Juan Carlos Mauricio,
  • Juan Moreno and
  • Teresa García-Martínez

A correlation between autophagy and autolysis has been proposed in order to accelerate the acquisition of wine organoleptic properties during sparkling wine elaboration. In this context, a proteomic analysis was carried out in two industrial Saccharo...

(This article belongs to the Special Issue Yeast in Winemaking)
  • Article
  • Open Access
4 Citations
1,893 Views
20 Pages

Exploring Static Biological Aging as a Method for Producing Low-Alcohol ‘Fino’ Type White Wines

  • Raquel Muñoz-Castells,
  • Lourdes Vega-Espinar,
  • Juan Carlos García-García,
  • Maria Trinidad Alcalá-Jiménez,
  • Jaime Moreno-García,
  • Cristina Lasanta and
  • Juan Moreno

Spanish “Fino”-style white wines are traditionally aged by a dynamic process under a flor veil of Saccharomyces cerevisiae, requiring ≥15% (v/v) ethanol, which is typically achieved through fortification. Market demand for lower-alcoho...

(This article belongs to the Special Issue Scale-Up Challenges in Microbial Fermentation)
  • Article
  • Open Access
7 Citations
2,191 Views
15 Pages

Selection of Non-Saccharomyces Yeasts from Extreme Oenological Environments for Potential Use in Winemaking

  • María Trinidad Alcalá-Jiménez,
  • Juan Carlos García-García,
  • Juan Carlos Mauricio,
  • Juan Moreno,
  • Rafael Peinado and
  • Teresa García-Martínez

This study evaluated the oenological potential of two non-Saccharomyces yeast strains, Hanseniaspora opuntiae TR-5 and Zygosaccharomyces bailii L-25, isolated from extreme winemaking environments in southern Spain. Out of 156 yeast isolates screened...

(This article belongs to the Section Food Microbiology)
  • Article
  • Open Access
16 Citations
4,638 Views
12 Pages

Non-Saccharomyces Are Also Forming the Veil of Flor in Sherry Wines

  • Marina Ruiz-Muñoz,
  • María Hernández-Fernández,
  • Gustavo Cordero-Bueso,
  • Sergio Martínez-Verdugo,
  • Fernando Pérez and
  • Jesús Manuel Cantoral

Biological ageing is an essential process for obtaining some distinctive Sherry wines, such as Fino and Manzanilla. It occurs after the fermentation of the grape must due to the appearance of a biofilm on the surface of the wine called “veil of...

(This article belongs to the Special Issue Enological Repercussions of Non-saccharomyces Species 4.0)
  • Article
  • Open Access
24 Citations
5,473 Views
25 Pages

Analytical and Chemometric Characterization of Fino and Amontillado Sherries during Aging in Criaderas y Solera System

  • Manuel J. Valcárcel-Muñoz,
  • María Guerrero-Chanivet,
  • María del Carmen Rodríguez-Dodero,
  • María de Valme García-Moreno and
  • Dominico A. Guillén-Sánchez

7 January 2022

Fino and Amontillado are Sherry wines, produced in Marco de Jerez area (southern Spain), and aged in Criaderas y Solera system. Fino Sherry wine follows a biological aging process, under a veil of flor yeasts, while Amontillado Sherry wine shares the...

(This article belongs to the Special Issue Wine Chemistry: The Key behind Wine Quality—2nd Edition)
  • Article
  • Open Access
10 Citations
3,517 Views
29 Pages

Analytical, Chemometric and Sensorial Characterization of Oloroso and Palo Cortado Sherries during Their Ageing in the Criaderas y Solera System

  • Manuel J. Valcárcel-Muñoz,
  • María Guerrero-Chanivet,
  • Carmen Rodríguez-Dodero,
  • M. de Valme García-Moreno and
  • Dominico A. Guillén-Sánchez

15 December 2022

Oloroso and Palo Cortado are two types of sherry wines, produced in the Sherry Wine Region in Southern Spain, known as Marco de Jerez, where it is aged following the traditional Criaderas y Solera system. All of them are aged through oxidative ageing...

(This article belongs to the Section Food Physics and (Bio)Chemistry)
  • Correction
  • Open Access
1,506 Views
5 Pages

The authors wish to make the following corrections to this paper [...]

(This article belongs to the Section Microbial Metabolism, Physiology & Genetics)
  • Article
  • Open Access
247 Views
19 Pages

12 October 2026

The reduction in alcohol content in Protected Designation of Origin (P.D.O.) ‘Jerez-Xérès-Sherry’ biologically aged wines represents a major regulatory and commercial milestone to align these historic products with modern gl...

(This article belongs to the Special Issue Effects of Processing Technology on the Quality of Wine and Winemaking By-Products)