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  • Article
  • Open Access
32 Citations
7,461 Views
32 Pages

Metatranscriptome Analysis of Sympatric Bee Species Identifies Bee Virus Variants and a New Virus, Andrena-Associated Bee Virus-1

  • Katie F. Daughenbaugh,
  • Idan Kahnonitch,
  • Charles C. Carey,
  • Alexander J. McMenamin and ,
  • Tanner Wiegand,
  • Tal Erez,
  • Naama Arkin,
  • Brian Ross,
  • Blake Wiedenheft and
  • Michelle L. Flenniken
  • + 8 authors+ 3 authors

12 February 2021

Bees are important plant pollinators in agricultural and natural ecosystems. High average annual losses of honey bee (Apis mellifera) colonies in some parts of the world, and regional population declines of some mining bee species (Andrena spp.), are...

(This article belongs to the Special Issue Evolution and Diversity of Insect Viruses)
  • Opinion
  • Open Access
2 Citations
3,022 Views
10 Pages

28 February 2022

Bees, both wild and domesticated ones, are hosts to a plethora of viruses, with most of them infecting a wide range of bee species and genera. Although viral discovery and research on bee viruses date back over 50 years, the last decade is marked by...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
2 Citations
1,139 Views
6 Pages

Viruses Infecting Cuban Honey Bees and Evolution of Deformed-Wing-Virus Variants

  • Poppy J. Hesketh-Best,
  • Anais R. Luis,
  • Declan C. Schroeder and
  • Stephen J. Martin

22 January 2026

Cuba is in a unique situation in which it has a large (220,000 managed colonies) and isolated honey bee population due to a 60+ year ban on the importation of bees. Despite this, the ectoparasitic mite Varroa destructor arrived in 1996, and with it c...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
28 Citations
6,605 Views
11 Pages

24 May 2021

The combination of Deformed wing virus (DWV) and Varroa destructor is arguably one of the greatest threats currently facing western honey bees, Apis mellifera. Varroa’s association with DWV has decreased viral diversity and increased loads of DW...

(This article belongs to the Special Issue Viruses in Mass-Reared Invertebrates)
  • Article
  • Open Access
2 Citations
2,300 Views
27 Pages

Honey Bee Viromes from Beekeeping Operations Experiencing High Losses in 2022–2023

  • Boone H. Jones,
  • Taylor Reams,
  • Lauren Jonas,
  • Brandon K. Hopkins and
  • Michelle L. Flenniken

9 March 2026

Recent high annual losses of honey bee (Apis mellifera) colonies, averaging 40% in the United States from 2008 to 2025, are concerning for beekeepers, growers, policy makers, and scientists. Viruses, the most abundant group of honey bee pathogens, im...

(This article belongs to the Section Invertebrate Viruses)
  • Communication
  • Open Access
11 Citations
4,890 Views
6 Pages

Updating Sacbrood Virus Quantification PCR Method Using a TaqMan-MGB Probe

  • Wei-Fone Huang,
  • Yakun Zhang,
  • Shahid Mehmood,
  • Zhengwei Wang and ,
  • Chunsheng Hou and
  • Zhiguo Li
  • + 1 author+ 0 author

13 April 2021

Sacbrood virus (SBV) is a common honey bee virus disease. SBV variants and strains identified in Asian honey bees, Apis cerana, have created confusion in identifications. Although the regional names indicated the expansions of the virus in new region...

(This article belongs to the Special Issue Honey Bee Pathogens and Parasites)
  • Article
  • Open Access
5 Citations
4,770 Views
12 Pages

Intra-Colonial Viral Infections in Western Honey Bees (Apis Mellifera)

  • Loreley Castelli,
  • María Laura Genchi García,
  • Anne Dalmon,
  • Daniela Arredondo and ,
  • Karina Antúnez,
  • Ciro Invernizzi,
  • Francisco José Reynaldi,
  • Yves Le Conte and
  • Alexis Beaurepaire
  • + 4 authors+ 0 author

RNA viruses play a significant role in the current high losses of pollinators. Although many studies have focused on the epidemiology of western honey bee (Apis mellifera) viruses at the colony level, the dynamics of virus infection within colonies r...

(This article belongs to the Special Issue Microorganisms in Pollinators: Interactions with Other Factors)
  • Article
  • Open Access
5 Citations
2,605 Views
12 Pages

Verifying the inclusivity of molecular detection methods gives indications about the reliability of viral infection diagnosis because of the tendency of viral pathogens to undergo sequence variation. This study was aimed at selecting inclusive probes...

(This article belongs to the Section Microbial Biotechnology)
  • Article
  • Open Access
27 Citations
6,108 Views
20 Pages

Unraveling Honey Bee–Varroa destructor Interaction: Multiple Factors Involved in Differential Resistance between Two Uruguayan Populations

  • Yamandú Mendoza,
  • Ivanna H. Tomasco,
  • Karina Antúnez,
  • Loreley Castelli and ,
  • Belén Branchiccela,
  • Estela Santos and
  • Ciro Invernizzi
  • + 2 authors+ 0 author

20 August 2020

The ectoparasite Varroa destructor is the greatest biotic threat of honey bees Apis mellifera in vast regions of the world. Recently, the study of natural mite-resistant populations has gained much interest to understand the action of natural selecti...

(This article belongs to the Special Issue Honey Bee Health)
  • Article
  • Open Access
2 Citations
3,899 Views
24 Pages

Characterization of a Molecular Clone of Deformed Wing Virus B

  • Sandra Barth,
  • Sebastian Affeldt,
  • Claudia Blaurock,
  • Irmin Lobedank and ,
  • Anette Netsch,
  • Kerstin Seitz,
  • Till Rümenapf and
  • Benjamin Lamp
  • + 3 authors+ 0 author

18 June 2024

Honey bees (Apis mellifera) play a crucial role in agriculture through their pollination activities. However, they have faced significant health challenges over the past decades that can limit colony performance and even lead to collapse. A primary c...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
36 Citations
7,009 Views
16 Pages

RNAseq Analysis Reveals Virus Diversity within Hawaiian Apiary Insect Communities

  • Laura E. Brettell,
  • Declan C. Schroeder and
  • Stephen J. Martin

27 April 2019

Deformed wing virus (DWV) is the most abundant viral pathogen of honey bees and has been associated with large-scale colony losses. DWV and other bee-associated RNA viruses are generalists capable of infecting diverse hosts. Here, we used RNAseq anal...

(This article belongs to the Special Issue Virus Bioinformatics)
  • Article
  • Open Access
29 Citations
6,357 Views
20 Pages

Metagenomic Approach with the NetoVIR Enrichment Protocol Reveals Virus Diversity within Ethiopian Honey Bees (Apis mellifera simensis)

  • Haftom Gebremedhn,
  • Ward Deboutte,
  • Karel Schoonvaere,
  • Peter Demaeght and ,
  • Lina De Smet,
  • Bezabeh Amssalu,
  • Jelle Matthijnssens and
  • Dirk C. de Graaf
  • + 3 authors+ 0 author

27 October 2020

Metagenomics studies have accelerated the discovery of novel or divergent viruses of the honey bee. However, most of these studies predominantly focused on RNA viruses, and many suffer from the relatively low abundance of viral nucleic acids in the s...

(This article belongs to the Special Issue Evolution and Diversity of Insect Viruses)
  • Article
  • Open Access
14 Citations
6,567 Views
14 Pages

The Pathogen Profile of a Honey Bee Queen Does Not Reflect That of Her Workers

  • Jessica L. Kevill,
  • Katie Lee,
  • Michael Goblirsch,
  • Erin McDermott and ,
  • David R. Tarpy,
  • Marla Spivak and
  • Declan C. Schroeder
  • + 2 authors+ 0 author

20 June 2020

Throughout a honey bee queen’s lifetime, she is tended to by her worker daughters, who feed and groom her. Such interactions provide possible horizontal transmission routes for pathogens from the workers to the queen, and as such a queen’...

  • Article
  • Open Access
82 Citations
9,755 Views
14 Pages

ABC Assay: Method Development and Application to Quantify the Role of Three DWV Master Variants in Overwinter Colony Losses of European Honey Bees

  • Jessica L. Kevill,
  • Andrea Highfield,
  • Gideon J. Mordecai,
  • Stephen J. Martin and
  • Declan C. Schroeder

27 October 2017

Deformed wing virus (DWV) is one of the most prevalent honey bee viral pathogens in the world. Typical of many RNA viruses, DWV is a quasi-species, which is comprised of a large number of different variants, currently consisting of three master varia...

(This article belongs to the Special Issue Antiviral Defense in Invertebrates)
  • Article
  • Open Access
57 Citations
12,493 Views
12 Pages

A Comparison of Deformed Wing Virus in Deformed and Asymptomatic Honey Bees

  • Laura E. Brettell,
  • Gideon J. Mordecai,
  • Declan C. Schroeder,
  • Ian M. Jones and ,
  • Jessica R. Da Silva,
  • Marina Vicente-Rubiano and
  • Stephen J. Martin
  • + 2 authors+ 0 author

7 March 2017

Deformed wing virus (DWV) in association with Varroa destructor is currently attributed to being responsible for colony collapse in the western honey bee (Apis mellifera). The appearance of deformed individuals within an infested colony has long been...

(This article belongs to the Special Issue Interactions Among Threats to Honeybee Health)
  • Article
  • Open Access
3,624 Views
16 Pages

A DNA Plasmid-Based Approach for Efficient Synthesis of Sacbrood Virus Infectious Clones within Host Cells

  • Dandan Yue,
  • Runlin Li,
  • Jikailang Zhang,
  • Yanping Chen and ,
  • Evan C. Palmer-Young,
  • Shaokang Huang and
  • Wei-Fone Huang
  • + 2 authors+ 0 author

1 September 2023

RNA viruses are often cited as a significant factor affecting the populations of both domestic honey bees and wild pollinators. To expedite the development of effective countermeasures against these viruses, a more comprehensive understanding of viru...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
18 Citations
4,173 Views
16 Pages

1 October 2021

Insects have a highly sensitive sense of smell, allowing them to perform complex behaviors, such as foraging and peer recognition. Their sense of smell is based on the recognition of ligands and is mainly coordinated by odorant-binding proteins (OBPs...

(This article belongs to the Section Insect Behavior and Pathology)
  • Feature Paper
  • Article
  • Open Access
11 Citations
5,002 Views
12 Pages

Co-Occurrence of Wing Deformity and Impaired Mobility of Alates with Deformed Wing Virus in Solenopsis invicta Buren (Hymenoptera: Formicidae)

  • Godfrey P. Miles,
  • Xiaofen F. Liu,
  • Esmaeil Amiri,
  • Michael J. Grodowitz and ,
  • Margaret L. Allen and
  • Jian Chen
  • + 1 author+ 0 author

27 September 2023

Deformed wing virus (DWV), a major honey bee pathogen, is a generalist insect virus detected in diverse insect phyla, including numerous ant genera. Its clinical symptoms have only been reported in honey bees, bumble bees, and wasps. DWV is a quasisp...

(This article belongs to the Collection Biology of Social Insect Diseases)
  • Article
  • Open Access
5 Citations
2,940 Views
13 Pages

1 October 2023

Deformed wing virus (DWV) is one of the important pathogens of the honey bee (Apis mellifera), which consists of three master variants: types A, B, and C. Among them, DWV types A (DWV-A) and B (DWV-B) are the most prevalent variants in honey bee colo...

(This article belongs to the Section Invertebrate Viruses)
  • Article
  • Open Access
42 Citations
8,164 Views
17 Pages

Green Bees: Reverse Genetic Analysis of Deformed Wing Virus Transmission, Replication, and Tropism

  • Olesya N. Gusachenko,
  • Luke Woodford,
  • Katharin Balbirnie-Cumming,
  • Ewan M. Campbell and ,
  • Craig R. Christie,
  • Alan S. Bowman and
  • David J. Evans
  • + 2 authors+ 0 author

12 May 2020

Environmental and agricultural pollination services by honey bees, Apis mellifera, and honey production are compromised by high levels of annual colony losses globally. The majority are associated with disease caused by deformed wing virus (DWV), a p...

(This article belongs to the Special Issue Advances in Honey Bee Virus Research)
  • Article
  • Open Access
9 Citations
3,484 Views
19 Pages

15 November 2023

The Varroa destructor mite is a devastating parasite of honey bees; however the negative effects of varroa parasitism are exacerbated by its role as an efficient vector of the honey bee pathogen, Deformed wing virus (DWV). While no direct treatment f...

(This article belongs to the Section Viral Immunology, Vaccines, and Antivirals)
  • Article
  • Open Access
3 Citations
2,096 Views
13 Pages

Molecular Characterization and Phylogenetic Analysis of Honeybee (Apis mellifera) Mite-Borne Pathogen DWV-A and DWV-B Isolated from Lithuania

  • Paulina Amšiejūtė-Graziani,
  • Vaclovas Jurgelevičius,
  • Simona Pilevičienė,
  • Žygimantas Janeliūnas and ,
  • Jana Radzijevskaja,
  • Algimantas Paulauskas,
  • Česlova Butrimaitė-Ambrozevičienė and
  • Ingrida Jacevičienė
  • + 3 authors+ 0 author

Deformed wing virus (DWV) is known as one of the main viruses that affect honeybees’ health all around the world. The virus has two widespread genotypes, DWV-A and DWV-B (VDV-1), transmitted mainly by V. destructor mites. In this study, we coll...

(This article belongs to the Special Issue Animal Virology, Molecular Diagnostics and Vaccine Development)
  • Article
  • Open Access
953 Views
16 Pages

Distribution and Quantification of Infectious and Parasitic Agents in Managed Honeybees in Central Italy, the Republic of Kosovo, and Albania

  • Franca Rossi,
  • Martina Iannitto,
  • Beqe Hulaj,
  • Luciano Ricchiuti and ,
  • Ani Vodica,
  • Patrizia Tucci,
  • Franco Mutinelli and
  • Anna Granato
  • + 3 authors+ 0 author

This study aimed to determine the presence of relevant infectious and parasitic agents (IPAs) in managed honeybees from Central Italy and the Republic of Kosovo and Albania to assess the overall health status of local apiaries by determining the cont...

(This article belongs to the Special Issue Infectious Diseases in Animals)
  • Article
  • Open Access
96 Citations
10,613 Views
12 Pages

DWV-A Lethal to Honey Bees (Apis mellifera): A Colony Level Survey of DWV Variants (A, B, and C) in England, Wales, and 32 States across the US

  • Jessica L. Kevill,
  • Flaviane S. de Souza,
  • Christopher Sharples,
  • Randy Oliver and ,
  • Declan C. Schroeder and
  • Stephen J. Martin
  • + 1 author+ 0 author

9 May 2019

The strong association between Varroa destructor, deformed wing virus (DWV), and high overwintering colony losses (OCL) of honey bees is well established. Three DWV master variants (DWV-A, -B, and -C) have been described, and their role in colony mor...

(This article belongs to the Special Issue Insect Viruses and Pest Management)
  • Article
  • Open Access
7 Citations
4,023 Views
15 Pages

Nursing Honeybee Behavior and Sensorial-Related Genes Are Altered by Deformed Wing Virus Variant A

  • Silva Diego,
  • Arismendi Nolberto,
  • Alveal Juan Pablo,
  • Ceballos Ricardo and ,
  • Zapata Nelson and
  • Vargas Marisol
  • + 1 author+ 0 author

23 January 2024

Insect behavior is coordinated mainly by smell through the diverse odor-binding proteins (OBP) that allow them to identify and recognize their environment. Sensory information collected through smell is then analyzed and interpreted in the brain, all...