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Photobiological Hydrogen Production in Cyanobacteria: Advances, Challenges, and Perspectives -
Comprehensive Changes in Phytochemical and Biological Activities Through the Fermentation Periods of Mul-Kimchi with Bitter Melon (Momordica charantia L.) -
Untargeted Metabolomics in Fermented Food Systems -
A Blend of Essential Oils (Blend of Eugenol, Linalool, Anethole, and Cinnamaldehyde) Increases Ruminal Propionate and Improves Total Tract Starch Digestibility in Steers Fed a Dry-Rolled Corn-Based Finishing Diet -
Advances in the Biosynthetic Production of Daunomycin: Genetic, Metabolic, and Process Engineering Strategies
Journal Description
Fermentation
Fermentation
is an international, peer-reviewed, open access journal on fermentation process and technology, published monthly online by MDPI.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), PubAg, FSTA, Inspec, CAPlus / SciFinder, and other databases.
- Journal Rank: JCR - Q2 (Biotechnology and Applied Microbiology) / CiteScore - Q1 (Plant Science)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 16.8 days after submission; acceptance to publication is undertaken in 3.6 days (median values for papers published in this journal in the first half of 2026).
- Recognition of Reviewers: reviewers who provide timely, thorough peer-review reports receive vouchers entitling them to a discount on the APC of their next publication in any MDPI journal, in appreciation of the work done.
- Journal Cluster of Microbiology: Acta Microbiologica Hellenica, Applied Microbiology, Bacteria, Journal of Fungi, Microorganisms, Microbiology Research, Pathogens, Viruses, Fermentation and Germs.
Impact Factor:
4.1 (2025);
5-Year Impact Factor:
4.3 (2025)
Latest Articles
Effects of Controlled Partial Fermentation on the Composition and Sensory Profile of Pedro Ximénez Wines
Fermentation 2026, 12(8), 393; https://doi.org/10.3390/fermentation12080393 - 21 Aug 2026
Abstract
Pedro Ximénez sweet wines are traditionally produced from raisined grapes and are characterised by very high sugar and ethanol contents. However, there is growing interest in wines with lower sweetness and improved sensory balance. The aim of this study was to evaluate the
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Pedro Ximénez sweet wines are traditionally produced from raisined grapes and are characterised by very high sugar and ethanol contents. However, there is growing interest in wines with lower sweetness and improved sensory balance. The aim of this study was to evaluate the effect of controlled partial fermentation of Pedro Ximénez raisined grape must prior to fortification on the chemical and sensory properties of the resulting wines. Fermentations were conducted until ethanol concentrations of 3.0, 5.0, 6.5, and 7.7% (v/v) were reached, after which fermentation was arrested, and all wines were standardized to a final ethanol concentration of 9% (v/v). Fermentation progression resulted in a reduction of residual sugar content from 445 to 313 g/L and significant increases in titratable acidity, volatile acidity, glycerol, higher alcohols, ethyl acetate, and 2-phenylethanol. Hierarchical cluster analysis confirmed that fermentation degree was the main factor driving wine differentiation. Sensory evaluation showed that partial fermentation reduced the intensity of characteristic descriptors such as honey, raisin, and fig while simultaneously decreasing sweetness perception and increasing freshness and overall balance. Wines fermented to 6.5 and 7.7% (v/v) ethanol received the highest preference scores from the expert sensory panel. These results indicate that controlled partial fermentation is a feasible technological strategy for modifying the composition and sensory profile of Pedro Ximénez wines and provide a basis for further optimization of alternative, less sweet Pedro Ximénez-style wines.
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(This article belongs to the Special Issue Wine and Beer Fermentation, 3rd Edition)
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Open AccessSystematic Review
Progress in Additives That Promote Humification During Agricultural Waste Composting
by
Qian Zhang, Zonglu Yao, Lixin Zhao, Jing Feng, Juan Luo, Jiadong Yu and Ruixia Shen
Fermentation 2026, 12(8), 392; https://doi.org/10.3390/fermentation12080392 - 21 Aug 2026
Abstract
Aerobic composting converts agricultural waste into stable, humus-rich products, and the application of exogenous additives is an efficient strategy to enhance humification. This systematic review synthesized studies published between 2023 and 2026 on additives that promote humification during agricultural waste composting. Based on
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Aerobic composting converts agricultural waste into stable, humus-rich products, and the application of exogenous additives is an efficient strategy to enhance humification. This systematic review synthesized studies published between 2023 and 2026 on additives that promote humification during agricultural waste composting. Based on the distribution of the retrieved literature, additives are categorized into inorganic additives, organic additives, biological strategies, and composite systems, and the effects and mechanisms of each category are systematically discussed. Iron-based additives achieve the highest humic acid (HA) increases of 82–267% through Fenton-like redox catalysis. Clay minerals and biochar produce moderate HA enhancements of 25–163% via physical structuring and surface adsorption with broader applicability. Small-molecule precursors and exogenous humic substances achieve HA gains exceeding 100% at sub-percent doses. Biological strategies provide self-sustaining catalytic activity but are sensitive to environmental conditions. Composite additives, the largest category, generally outperform single additives through functional complementarity, though antagonistic effects have also been documented. Cross-study patterns suggest that different feedstocks respond preferentially to distinct additive types, though systematic experimental validation is lacking. Critical gaps between laboratory findings and practical application are identified, including the predominance of small-scale studies, the absence of techno-economic analysis, and the unassessed environmental fate of metal-based additives. Future research priorities include pilot-scale validation under industrial conditions, the establishment of standardized humification metrics, and long-term field monitoring.
Full article
(This article belongs to the Special Issue Fermented Biofertilizer Production and Application)
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Open AccessReview
A Review on Modeling the Fermentation Process of Dairy Products Using Multi-Omics and Artificial Intelligence Approaches
by
Murat Emre Terzioğlu and Zeynep Çağla Tekgül
Fermentation 2026, 12(8), 391; https://doi.org/10.3390/fermentation12080391 - 19 Aug 2026
Abstract
In dairy production, the fermentation process is a complex biochemical system that plays a significant role in determining the quality criteria of the final product. Traditional methods for controlling fermentation rely on limited and non-standard process parameters. In recent years, omics technologies have
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In dairy production, the fermentation process is a complex biochemical system that plays a significant role in determining the quality criteria of the final product. Traditional methods for controlling fermentation rely on limited and non-standard process parameters. In recent years, omics technologies have come to the forefront, enabling the monitoring of fermentation dynamics at the molecular level with their current, efficient, and reliable approaches. Thanks to omics approaches such as metabolomics, metagenomics, proteomics, and lipidomics, starter culture behavior, metabolite formation, aroma–texture formation, and microbial interactions in the fermentation process can be characterized more comprehensively. On the other hand, evaluating or calculating high-dimensional omics data using traditional statistical methods presents a challenge. Artificial intelligence applications are overcoming this challenge, offering significant opportunities for the accurate and reliable evaluation of data. Artificial intelligence-powered models hold promise in areas such as predicting fermentation kinetics, process control, optimizing quality criteria, and revealing the therapeutic potential of products through metabolites. This compilation aims to comprehensively address current approaches to modeling the fermentation process and quality parameters of dairy products using multi-omics technologies and artificial intelligence applications. In this respect, it will provide current and important perspectives for industrial applications and future studies.
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(This article belongs to the Special Issue Dairy Fermentation from a Microbial Perspective)
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Open AccessArticle
Fermentation Temperature as a Strategy to Limit Hanseniaspora uvarum Proliferation During Spontaneous Wine Fermentation
by
Cristobal A. Onetto, Jane McCarthy and Simon A. Schmidt
Fermentation 2026, 12(8), 390; https://doi.org/10.3390/fermentation12080390 - 19 Aug 2026
Abstract
Hanseniaspora uvarum is consistently observed as the dominant non-Saccharomyces species in grape juice leading to spontaneous fermentations and is often considered a detrimental factor due to the increased volatile acidity associated with its abundance. This study investigated how fermentation temperature affects the
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Hanseniaspora uvarum is consistently observed as the dominant non-Saccharomyces species in grape juice leading to spontaneous fermentations and is often considered a detrimental factor due to the increased volatile acidity associated with its abundance. This study investigated how fermentation temperature affects the competitive interaction between H. uvarum and Saccharomyces cerevisiae, and how these shifts influence microbial community structure during spontaneous grape juice fermentations. Defined single- and co-inoculated fermentations were conducted across a range of temperatures (17, 23, and 25 °C), and cell abundance was quantified by flow cytometry. In parallel, spontaneous fermentations were monitored using ITS metabarcoding to assess temperature-driven changes in fungal community composition. At 17 °C, H. uvarum dominated early fermentation due to its faster growth rate, resulting in slower sugar consumption and prolonged fermentations. Increasing fermentation temperature enhanced the early growth of S. cerevisiae. Consistent patterns were observed in spontaneous fermentations, where higher temperatures increased the relative abundance of S. cerevisiae and shortened fermentation time. These results demonstrate that fermentation temperature is a major driver of yeast competition and suggest that moderate increases in fermentation temperature may help limit excessive H. uvarum proliferation during spontaneous fermentations.
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(This article belongs to the Special Issue Wine and Beer Fermentation, 3rd Edition)
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Open AccessArticle
Co-Digestion as a Strategy to Optimize Anaerobic Digestion Without Pretreatment: Implications for Methane Yield and Process Stability
by
Aytac Perihan Akan, Kenan Dalkilic and Aysenur Ugurlu
Fermentation 2026, 12(8), 389; https://doi.org/10.3390/fermentation12080389 - 19 Aug 2026
Abstract
Rapid population growth, urbanization, and industrialization are continuously increasing global energy demand while intensifying climate change associated with fossil fuel consumption. In this context, renewable energy production from organic waste has gained increasing attention as a sustainable and environmentally friendly strategy. Anaerobic digestion
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Rapid population growth, urbanization, and industrialization are continuously increasing global energy demand while intensifying climate change associated with fossil fuel consumption. In this context, renewable energy production from organic waste has gained increasing attention as a sustainable and environmentally friendly strategy. Anaerobic digestion (AD) offers significant potential for simultaneous waste stabilization and biomethane generation. However, many previous studies investigating lignocellulosic or nutrient-rich substrates have relied on physical, chemical, or thermal pretreatment methods to enhance biodegradability, despite their additional operational costs, energy consumption, and environmental impacts. Therefore, developing low-cost and pretreatment-free co-digestion strategies remains an important research need. This study investigated the biomethane production potentials of untreated chicken manure (CM) and duckweed (Lemna minor-LM) collected from the final sedimentation tanks of wastewater treatment plants under mono-digestion and co-digestion conditions. The study hypothesized that rapidly growing and widely available LM biomass could enhance methane production without requiring pretreatment. Among all reactors, CM0.75 (75% of the total TS derived from CM and 25% from LM and inoculum) achieved the highest performance with a cumulative biogas production of 5350 mL (74.2% of CH4) and a methane yield of 327 mL CH4/g VS, while mono-digestion of CM resulted in the lowest methane yield of 104 mL CH4/g VS. The results demonstrated that LM biomass naturally proliferating in wastewater treatment plants can be directly utilized as an effective co-substrate to improve biomethane production from poultry wastes. The proposed approach provides a cost-efficient, eco-friendly, and circular-economy-oriented alternative by eliminating the need for pretreatment while simultaneously valorizing problematic biomass generated in wastewater treatment facilities.
Full article
(This article belongs to the Special Issue Fermentation of Organic Waste for High-Value-Added Product Production—2nd Edition)
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Open AccessArticle
Effects of Cold Fermentation and Cold Storage on Type I Sourdough and Bread Properties
by
Gulhan Turk, Gorkem Ozulku, Saeideh S. Fatemizadeh, Osman Sagdic and Ömer Şimşek
Fermentation 2026, 12(8), 388; https://doi.org/10.3390/fermentation12080388 - 18 Aug 2026
Abstract
The aim of this study is to provide an alternative bakery practice by evaluating the effects of cold fermentation (backslopping at 7 and 15 °C) and cold storage (stored at 7 and 15 °C) after backslopping on Type I sourdough characteristics and the
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The aim of this study is to provide an alternative bakery practice by evaluating the effects of cold fermentation (backslopping at 7 and 15 °C) and cold storage (stored at 7 and 15 °C) after backslopping on Type I sourdough characteristics and the technological performance of the corresponding breads. Sourdough characteristics (pH, acidity, and microbial counts), bread quality (texture and color), storage behavior, and volatile compound profiles (VoC) were investigated to compare the extent to which these treatments improve product quality. No significant difference was observed in pH and total titratable acidity among the sourdoughs. However, the fermentation quotient of the cold storage group was higher than that of the cold fermented group. Backslopping at cold fermentation temperatures (7 and 15 °C) caused a significant reduction in lactic acid bacteria counts. Conversely, cold fermentation and cold storage at 15 °C led to a significant increase in yeast counts. From a technological perspective, bread produced with sourdough backslopped at 15 °C exhibited a higher specific volume than breads produced with the other cold-treated sourdoughs. At the end of the storage period, the bread made with cold-backslopped sourdough at 7 °C had the highest hardness value, while others remained similar. Principal component analysis (PCA) of volatile compounds revealed that cold-stored sourdoughs were clustered apart from room-temperature-stored sourdoughs, mainly driven by ethyl hexanoate and hexyl acetate. Heptanoic acid was among the key VOCs contributing to the positioning of breads made with cold-stored sourdoughs in the PCA. In conclusion, these findings demonstrate that backslopping and storage at 15 °C offer an industrially relevant production strategy while improving textural properties and VoC profiles.
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(This article belongs to the Special Issue Biotechnology for Smarter Industrial Fermentation)
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Open AccessArticle
Evaluation of Monascus ruber Inoculum Preparation Strategies and Surfactant Supplementation to Enhance Biopigment Production in a Xylose-Based Medium Derived from Ethanol Biorefinery By-Products
by
Willian de S. M. Reis, Gabriel L. de Arruda, Silvio S. da Silva, Arnaldo M. R. Prata and Júlio C. dos Santos
Fermentation 2026, 12(8), 387; https://doi.org/10.3390/fermentation12080387 - 17 Aug 2026
Abstract
Bioenergy biorefineries generate lignocellulosic by-products rich in fermentable sugars that can serve as renewable feedstocks for the production of high-value bioproducts, including microbial pigments with promising bioactive properties (antioxidant, antimicrobial, and anticancer). Therefore, this study aimed to identify a suitable inoculum preparation strategy,
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Bioenergy biorefineries generate lignocellulosic by-products rich in fermentable sugars that can serve as renewable feedstocks for the production of high-value bioproducts, including microbial pigments with promising bioactive properties (antioxidant, antimicrobial, and anticancer). Therefore, this study aimed to identify a suitable inoculum preparation strategy, to optimize the culture medium, and to evaluate biopigment production using xylose-based media derived from sugarcane bagasse hemicellulosic hydrolysate (SBHH). Different inoculation strategies were evaluated (cell suspension, whole mycelial discs, and fractionated mycelial discs) and supplementation with Tween 80 (TW80). The medium composition was optimized using a Box–Behnken design, with xylose, yeast extract, and TW80 as variables, and fermentations were then conducted under selected inoculum conditions in semi-defined media and SBHH. The mycelial disc inoculation strategy was selected due to its high biopigment production and lower operational complexity, yielding 8.99, 8.48, and 11.78 AU of yellow, orange, and red biopigments, respectively. The optimized culture composition consisted of 55.65 g/L of xylose, 4.18 g/L of yeast extract, and 15.38 g/L of Tween 80. The cultivation of M. ruber in SBHH resulted in 12.73, 10.75, and 14.56 AU of yellow, orange, and red biopigments, respectively. Thus, the strategy of inoculum preparation associated with non-ionic surfactant proved promising for application in bioenergy biorefineries.
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(This article belongs to the Special Issue Microbial Processes for Biomass Conversion to Bioenergy)
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Open AccessArticle
Vitis vinifera Leaf Extract as a Sustainable Alternative to Sulphur Dioxide in High-Hydrostatic-Pressure-Treated Fiano Wine
by
Mamica Ruci, Renata Kongoli, Rosaria Cozzolino, Cristina Matarazzo, Bruno Testa, Onejda Kyçyk, Julian Karaulli, Massimo Di Renzo, Catello Di Martino, Fatbardha Lamçe and Massimo Iorizzo
Fermentation 2026, 12(8), 386; https://doi.org/10.3390/fermentation12080386 - 15 Aug 2026
Abstract
The growing demand for clean-label and low-sulphite wines has increased interest in alternative preservation strategies capable of reducing sulphur dioxide (SO2) usage while maintaining wine quality and stability. In this study, the effectiveness of Vitis vinifera leaf extract as a natural
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The growing demand for clean-label and low-sulphite wines has increased interest in alternative preservation strategies capable of reducing sulphur dioxide (SO2) usage while maintaining wine quality and stability. In this study, the effectiveness of Vitis vinifera leaf extract as a natural alternative to sulphur dioxide was evaluated in High Hydrostatic Pressure (HHP)-treated Fiano wines. Three experimental wines were produced: CW (control wine), SW (sulphited wine), and LW (leaf-extract wine). Following alcoholic fermentation, all wines were subjected to HHP treatment (600 MPa for 5 min) and stored at 4 °C for 60 days. Physicochemical parameters, volatile organic compounds (VOCs), and sensory characteristics were evaluated immediately after alcoholic fermentation and after HHP treatment followed by refrigerated storage. Compared with the control wine, LW exhibited approximately 20% higher total polyphenol concentrations, whereas SW showed the greatest preservation of fermentation-derived esters. HHP treatment induced only moderate changes in the volatile fraction, confirming the suitability of this non-thermal technology for wine stabilization. LW wines were characterized by higher abundances of terpene-related compounds, C6 alcohols, medium-chain fatty acids, and phenolic-associated volatiles, resulting in more pronounced floral, balsamic, herbaceous, and vegetal sensory attributes. Principal Component Analysis (PCA) explained 79.0% of the total VOC variability and clearly differentiated LW wines from CW and SW according to their volatile profiles, while sensory analysis confirmed the development of a distinctive aromatic identity associated with grapevine leaf extract. Overall, the results indicate that the combined application of V. vinifera leaf extract and HHP represents a promising strategy for the partial replacement of sulphur dioxide in white winemaking. This integrated approach contributes to wine stabilization while promoting the valorization of grapevine leaves as a sustainable winery by-product within a circular economy framework.
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(This article belongs to the Special Issue Recent Advances in the Fermentation of Plant-Based Foods and Beverages)
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Open AccessArticle
Effects of Hyperbaric Micro-Oxygenation on the Colour, Total Phenolic Content, Volatile Composition, and Sensory Profile of Vitis vinifera L. cv. Monastrell Grape Must
by
Pablo Mompean, José Ramón Acosta-Motos, Llanos Martínez-Martínez, Luis Noguera-Artiaga, Angel A. Carbonell-Barrachina, Patricia Navarro and Antonio José Pérez-López
Fermentation 2026, 12(8), 385; https://doi.org/10.3390/fermentation12080385 - 15 Aug 2026
Abstract
Oxygen management during alcoholic fermentation can redirect phenolic reactions and yeast-derived aroma formation, but the use of mild hyperbaric conditions as a micro-oxygenation strategy remains poorly characterized. This study evaluated the effects of hyperbaric micro-oxygenation at 1.40 ATA in a stainless-steel chamber on
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Oxygen management during alcoholic fermentation can redirect phenolic reactions and yeast-derived aroma formation, but the use of mild hyperbaric conditions as a micro-oxygenation strategy remains poorly characterized. This study evaluated the effects of hyperbaric micro-oxygenation at 1.40 ATA in a stainless-steel chamber on the fermentation of Vitis vinifera L. cv. Monastrell must, comparing treated and non-micro-oxygenated samples at the initial, mid-fermentation, and final stages. Physicochemical parameters, CIELAB color coordinates, total phenolic content, volatile organic compounds, and descriptive sensory attributes were analyzed. Hyperbaric micro-oxygenation did not impair fermentation completion, as both treatments reached final residual sugar values of 2.2 g/L and alcohol contents of 15.2–15.4% v/v. The treatment promoted a darker final chromatic profile, with lower L*, the highest overall color difference, and a marked increase in total phenolic content, reaching 1900.9 mg gallic acid equivalents/L compared with 1593.2 mg gallic acid equivalents/L in control. Volatile changes were compound, and stage-dependent, indicating modulation rather than generalized enhancement of aroma formation. Ethyl esters, particularly ethyl octanoate and ethyl decanoate, increased markedly under micro-oxygenation, while acetate esters such as ethyl acetate and hexyl acetate decreased relative to the initial must, reflecting a shift in the balance of aroma-active compounds rather than a uniform increase across all volatile families. These findings support mild hyperbaric micro-oxygenation as a promising non-thermal strategy to modulate Monastrell fermentation quality.
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(This article belongs to the Section Fermentation for Food and Beverages)
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Open AccessArticle
Performance of a Continuous Dark–Photo Fermentation System to Produce Hydrogen from Simulated Sugar–Rich Processing Wastewater Under Suboptimal pH and Temperature Conditions
by
Soumya Gupta, Annabel Fernandes, Laura Grasa, Carlos Rubio and Jesús Salafranca
Fermentation 2026, 12(8), 384; https://doi.org/10.3390/fermentation12080384 - 13 Aug 2026
Abstract
The integration of coupled dark fermentation (DF) and photofermentation (PF) systems represents a promising approach for concurrent H2 production and organic load reduction in carbohydrate–rich wastewater. However, their behavior under non–optimized, continuous industrial operation remains inadequately characterized, hindering practical implementation. This case
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The integration of coupled dark fermentation (DF) and photofermentation (PF) systems represents a promising approach for concurrent H2 production and organic load reduction in carbohydrate–rich wastewater. However, their behavior under non–optimized, continuous industrial operation remains inadequately characterized, hindering practical implementation. This case study evaluated an integrated DF–PF system treating a synthetic sugar mixture mimicking acidic fruit and dairy processing wastewater. The system presented herein (5-L reactors) serves as an initial prototype to facilitate scaling to both pilot (40-L reactors) and, ultimately, industrial (600-L) scales using real effluents within the framework of a research project. The bioreactors were operated continuously with a 10 h hydraulic retention time and an organic loading rate of 2.5 g COD L−1 d−1. The pH and temperature were monitored but intentionally left uncontrolled. The DF stage facilitated by hydrogen–producing bacteria achieved H2 concentrations of up to 57% (v/v) and a maximum production rate of 177 mL H2 L−1 d−1; however, it demonstrated notable process instability due to the absence of controls. In contrast, the PF stage exhibited negligible H2 production (1.8% v/v) attributable to the displacement of Rhodopseudomonas species by microbial competition, thereby functioning predominantly as a polishing step. Overall, the coupled system achieved an average COD removal of 34%, highlighting the functional differentiation between stages and identifying microbial competition as the primary constraint under suboptimal conditions.
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(This article belongs to the Special Issue Recent Advancements in Fermentation Technology: Biofuels Production)
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Enzymatically Pretreated Red-Fleshed Pitaya (Hylocereus polyrhizus) as a Co-Fermentation Substrate Improves the Nutritional Quality and Sensory Profile of Black Rice Wine
by
Chaoyang Zhu, Dina Zhu, Bei Liao, Zhanlei Fan, Yongmei Hu, Shumiao Zhao, Yunxiang Liang and Jinshan Li
Fermentation 2026, 12(8), 383; https://doi.org/10.3390/fermentation12080383 - 12 Aug 2026
Abstract
Black rice wine is a traditional cereal-based fermented beverage valued for its colour, flavour and bioactive constituents. However, product innovation is needed to meet increasing consumer demand for fruit-derived aroma, nutritional quality and functional attributes. To address this demand, we developed an integrated
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Black rice wine is a traditional cereal-based fermented beverage valued for its colour, flavour and bioactive constituents. However, product innovation is needed to meet increasing consumer demand for fruit-derived aroma, nutritional quality and functional attributes. To address this demand, we developed an integrated processing strategy combining enzymatic pretreatment of pitaya pulp with stage-specific co-fermentation, an approach that has not been systematically evaluated in rice wine systems. Pitaya pulp was first pretreated with pectinase using an orthogonal design to optimise juice yield and betacyanin retention; the enzymatically pretreated pitaya pulp was then introduced either as pulp or as fermented pitaya wine at the saccharification, tank-flushing or ageing stage. The resulting wines were compared with a pitaya-free control and three commercial black rice wines on the basis of physicochemical indices, phenolic and pigment contents, amino acid composition, mineral composition and fuzzy mathematics-based sensory evaluation. Pitaya pulp added at saccharification (Sample THJ) yielded the strongest functional and nutritional profile, with the highest total phenolic content (629.74 mg/L); high flavonoid content (152.07 mg/L); enrichment in Mg, K and Mn; and a broader taste-active amino acid profile. By contrast, pitaya pulp added during tank flushing (Sample CHJ) achieved the highest sensory score (85.3) and had the highest anthocyanin content. These findings indicate that the timing and form of pitaya addition determine the balance between functional enrichment, pigment retention and sensory quality. Co-fermentation with red-fleshed pitaya, particularly the early addition of enzymatically pretreated pitaya pulp, provides a practical route for the development of fruit-flavoured black rice wine with improved nutritional composition and consumer appeal, suggesting the potential for enhanced functional value.
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(This article belongs to the Special Issue Recent Advances in the Fermentation of Plant-Based Foods and Beverages)
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Open AccessArticle
Effect of Genotype and Microbial Inoculants on Quality, Fermentation, and In Vitro Digestibility of Barley at Early Dough Stage of Maturity
by
Umair Ahsan, Derya Merve Karagöz, Muhammad Shazaib Ramay, Syed Umer Akhter, Anas Tahir, Bekir Tosun, Ifrah Raza, Murat Er, Muhammad Kashif Yar and Eren Kuter
Fermentation 2026, 12(8), 382; https://doi.org/10.3390/fermentation12080382 - 12 Aug 2026
Abstract
A 2 × 2 × 2 factorial experiment was conducted to study the individual and combined effects of barley variety (Tarım 92 and Bravo), homofermentative inoculant (HMF; Lactobacillus plantarum and Enterococcus faecium), and heterofermentative inoculant (HTF; Lactobacillus buchneri) on the fermentation
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A 2 × 2 × 2 factorial experiment was conducted to study the individual and combined effects of barley variety (Tarım 92 and Bravo), homofermentative inoculant (HMF; Lactobacillus plantarum and Enterococcus faecium), and heterofermentative inoculant (HTF; Lactobacillus buchneri) on the fermentation characteristics, chemical composition, nutritive value indices, and in vitro digestibility of whole-crop barley silages which were harvested at the early dough stage. Forty-eight laboratory silos (six replicates per treatment) were ensiled for 120 days. Tarım 92 silage exhibited superior fermentation stability across all treatments, with pH ranging from 4.11 to 4.30, Flieg points of 102.94–115.47, and lower NH3-N concentrations (142.01–173.72 mg/kg TN (total nitrogen)), reflecting better protein preservation than Bravo silage. In contrast, the poorest fermentation outcome (pH 5.24; Flieg 47.06; NH3-N 307.48 mg/kg TN) was exhibited by the Bravo silage inoculated with only homofermentative inoculants. Significant three-way interaction effects were detected among barley variety, homofermentative inoculant, and heterofermentative inoculant (p < 0.05). The dual-inoculated Tarım 92 silage showed the optimum in vitro digestibility and nutritional value (TDN 77.14%; NEL 1.81 Mcal kg−1 DM; RFV 176.25; ADF 18.75%; IVOMD 65.72%; predicted DMI 3.06% BW), while higher amounts of butyric acid and NH3-N suggested that better nutritional value was not always accompanied by better protein preservation. The combined inoculation also yielded the best outcomes within Bravo treatments (RFV 144.00), whereas HTF inoculation alone reduced IVDMD and IVOMD below uninoculated controls. Overall, these results show that the response to microbial inoculation is genotype-dependent and a combination of homofermentative and heterofermentative inoculation for high-dry-matter barley varieties can enhance nutritive value and digestibility. However, fermentation quality indicators, particularly butyric acid and NH3-N, require consideration in the selection of technique.
Full article
(This article belongs to the Special Issue Application of Fermentation Technology in Animal Nutrition: 3rd Edition)
Open AccessArticle
Effects of Molasses-Based Liquid Feeds Containing Conventional Urea or Commercial Fat-Coated Urea Product on In Vitro Rumen Fermentation, Gas Kinetics, and Substrate Degradability
by
Yotsapon Yangngam, Seangla Cheas, Chanon Suntara, Metha Wanapat, Juan J. Loor and Anusorn Cherdthong
Fermentation 2026, 12(8), 381; https://doi.org/10.3390/fermentation12080381 - 11 Aug 2026
Abstract
This study evaluated molasses-based liquid feeds containing conventional urea or a commercial fat-coated urea product marketed as slow-release urea (SRU). The effects on in vitro gas-production kinetics, rumen fermentation, and substrate degradability were examined. Treatments followed a 5 × 3 factorial design. The
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This study evaluated molasses-based liquid feeds containing conventional urea or a commercial fat-coated urea product marketed as slow-release urea (SRU). The effects on in vitro gas-production kinetics, rumen fermentation, and substrate degradability were examined. Treatments followed a 5 × 3 factorial design. The five formulations were 0% urea, 5% urea, 10% urea, 5% SRU, and 10% SRU. Each formulation was supplied at 0.9%, 1.8%, or 2.7% of the 0.5 g basal substrate dry matter (DM). Four independent incubation runs were conducted. A formulation × inclusion-level interaction was detected for gas-production lag time (p = 0.0046). The shortest lag time occurred with 10% SRU supplied at 2.7%. At 48 h, the 0.9% inclusion level resulted in greater dry matter and organic matter degradability than the 1.8% and 2.7% levels. The 5% SRU formulation had the lowest 24 h degradability, whereas 10% SRU had the greatest 48 h dry matter degradability. Ammonia nitrogen (NH3-N) was analyzed separately at each sampling time. The 10% conventional urea formulation had the greatest NH3-N concentration at 1 and 2 h. The 10% SRU formulation had the greatest concentration at 4, 6, and 12 h. These within-time differences do not demonstrate different ammonia-release patterns over time. At 12 h, the 10% SRU formulation had the greatest total volatile fatty acid concentration and propionate molar proportion. Protozoal counts were unaffected. The results reflect differences among the complete liquid-feed formulations. They do not provide direct evidence of controlled ammonia release or improved nitrogen–carbohydrate synchronization. Further in vivo studies using compositionally balanced formulations are required.
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(This article belongs to the Special Issue Fermentation Technologies for Sustainable Animal Feed)
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Gut Microbiome and Metabolome Responses to Fermented Fish Paste (Kapi-pla) in a Simulated Colonic Model of Alzheimer’s Disease
by
Nisa Alfilasari, Nattha Tampanna, Nualpun Sirinupong and Santad Wichienchot
Fermentation 2026, 12(8), 380; https://doi.org/10.3390/fermentation12080380 - 11 Aug 2026
Abstract
Bioactive peptides (BPs) are increasingly recognized for modulating the gut microbiome, metabolome, and brain function via the gut–brain axis. Kapi-pla, a traditional Thai fermented freshwater fish paste rich in proteins and peptides, is widely consumed in Southern Thailand. This study profiled the peptides
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Bioactive peptides (BPs) are increasingly recognized for modulating the gut microbiome, metabolome, and brain function via the gut–brain axis. Kapi-pla, a traditional Thai fermented freshwater fish paste rich in proteins and peptides, is widely consumed in Southern Thailand. This study profiled the peptides of Phatthalung Kapi-pla (PK) and Songkhla Kapi-pla (SK) and investigated their impacts on gut microbiota and metabolome using a simulated colonic fermentation model with fecal samples from patients with Alzheimer’s disease (AD). Microbial composition and metabolites were assessed by 16S rRNA sequencing and LC–MS/MS, respectively. After 24 h fermentation, PK modestly increased Shannon diversity relative to the unsupplemented control, with richness indices unchanged, reduced Proteobacteria abundance and opportunistic pathogens such as Escherichia–Shigella and Klebsiella, and selectively increased short- and branched-chain fatty acids, including acetate, propionate, butyrate, and iso-valerate. PK further enhanced neuroactive metabolites relevant to AD pathology, underscoring its potential as a functional food ingredient to ameliorate AD-associated dysbiosis and support gut–brain axis health.
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(This article belongs to the Special Issue Emerging Microbial Technologies in Fermentation: Innovations in Food, Environmental, and Health Bioprocesses)
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Open AccessArticle
The Effect of Pre-Fermentative Thermo-Maceration and Apple Pomace Addition on Phenolic Content and Volatile Profile of Hard Cider
by
Luis F. Castro, Tessa A. J. Murphree, Kate P. Perry, Sean Kuster and L. Federico Casassa
Fermentation 2026, 12(8), 379; https://doi.org/10.3390/fermentation12080379 - 10 Aug 2026
Abstract
The aim of this study was to investigate the effects of thermo-maceration and apple pomace addition on phenolic compound extraction during cider fermentation and on the volatile profile of the final product. Jonagold apples were milled, and the juice was divided into four
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The aim of this study was to investigate the effects of thermo-maceration and apple pomace addition on phenolic compound extraction during cider fermentation and on the volatile profile of the final product. Jonagold apples were milled, and the juice was divided into four groups: (1) Control: no apple pomace or thermo-maceration; (2) Control TM: thermo-maceration only; (3) CiderAP: apple pomace addition only; and (4) CiderAPTM: apple pomace addition combined with thermo-maceration. Apple pomace was added at 50% of the average pomace weight created during milling, and thermo-maceration consisted of heating the juice to 60 °C for 1 h prior to fermentation. Ciders were fermented at 20 ± 1 °C for 14 days. CiderAP and CiderAPTM showed higher total phenolic content (TPC) and antioxidant activity than the control treatments. The volatile profile was also modified, with CiderAPTM showing the highest phenylethyl alcohol concentration and esters exhibiting compound-specific responses. Statistical analysis showed that only apple pomace addition significantly increased TPC and antioxidant activity, while both apple pomace addition and thermo-maceration affected the volatile profile. Under the conditions evaluated, thermo-maceration did not increase TPC or antioxidant activity beyond the effect of apple pomace addition but did modify the volatile profile of the ciders. Further research should evaluate different thermo-maceration temperatures and durations to determine its potential application in cider production. The findings of this study provide valuable insights for cider makers on the valorization of apple pomace and potential use of thermo-maceration in cider production.
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(This article belongs to the Section Fermentation for Food and Beverages)
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Open AccessArticle
Effects of Replacing Concentrate Feed with Carob (Ceratonia siliqua) Pods on Growth Performance, Carcass Characteristics, Meat Quality, and Rumen Fermentation in Assaf Lambs
by
Soha Ghzayel, Ahmed E. Kholif, Alexey Díaz-Reyes, Bassam Abu Aziz, Halimeh Zoabi, Raouia Ben Rhouma, Sawsan Hassan, Secundino López, Adel M. M. Kholif, Silvia Parrini, Andrea Confessore and Hajer Ammar
Fermentation 2026, 12(8), 378; https://doi.org/10.3390/fermentation12080378 - 10 Aug 2026
Abstract
This study examined the effects of replacing 25% (P25) or 50% (P50) of concentrate dry matter (DM) with sun-dried carob (Ceratonia siliqua L.) pods on growth performance, apparent nutrient digestibility, carcass traits, meat quality, serum biochemistry, and rumen microbiology in growing Assaf
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This study examined the effects of replacing 25% (P25) or 50% (P50) of concentrate dry matter (DM) with sun-dried carob (Ceratonia siliqua L.) pods on growth performance, apparent nutrient digestibility, carcass traits, meat quality, serum biochemistry, and rumen microbiology in growing Assaf lambs. Twenty-four weaned male Assaf lambs (initial body weight [BW] 27.0 ± 0.5 kg; 2.5 months of age) were randomly assigned to three dietary treatments (n = 8 per group) in a completely randomized design and fed for 16 weeks. P50 achieved the highest ANCOVA-adjusted least squares mean final BW (53.0 kg) and average daily gain (ADG) (220.8 g/d), followed by P25 (51.1 kg; 203.3 g/d) and the control (46.2 kg; 160.2 g/d) (p < 0.001). Feed conversion ratio (FCR) improved from 8.99 in the control to 6.16 and 6.11 in P25 and P50, respectively, with no significant difference between the two carob-supplemented groups (p < 0.001). Apparent DM and organic matter (OM) digestibility increased with carob inclusion at both 3 and 6 months of age (p ≤ 0.0001). Cold carcass weight (CCW) was higher in carob-supplemented lambs (p < 0.001), whereas carcass muscle proportion did not differ among treatments (p = 0.688), and carcass fat proportion was higher in P25 than in the control (p = 0.039). Warner–Bratzler shear force declined progressively with carob inclusion (p < 0.001), indicating improved meat tenderness. Serum total protein was highest in P25, whereas blood urea nitrogen (BUN), low-density lipoprotein (LDL), and glutamate oxaloacetate transaminase (GOT) decreased with carob inclusion (p < 0.001). Rumen pH was highest in P25 (6.40), total bacterial and lactic acid bacteria (LAB) counts increased, and protozoa counts declined (p < 0.001). Because carob pods replaced concentrate, rather than being added to an isonitrogenous diet, the combined effects of pods per se, reduced crude protein supply, and altered energy density must all be considered when interpreting the results. These findings support carob pods as a practical, locally available partial substitute for concentrate feed in Assaf lamb production under Mediterranean and Near Eastern conditions.
Full article
(This article belongs to the Special Issue Fermentation Technologies for Sustainable Animal Feed)
Open AccessArticle
Effects of Extraction Methods on Inulin, Fructooligosaccharides and Microbial Inulinase Production from Stevia rebaudiana
by
Gokce Kucuk, Muge Canatar, Selin Basmak, Selime Benemir Erkan Ünsal, Hilal Nur Gürler Tufan, Ali Ozcan, Mustafa Karhan, Ercan Yatmaz, İbrahim Yavuz and Irfan Turhan
Fermentation 2026, 12(8), 377; https://doi.org/10.3390/fermentation12080377 - 10 Aug 2026
Abstract
In this study, Stevia fractions (root, leaf, stem and their mixture) were used for inulin extraction using aqueous extraction, enzyme-assisted extraction and enzyme-assisted decanter extraction. The extracts were used as carbon sources in submerged (SmF) fermentation, whereas the remaining solid residues were used
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In this study, Stevia fractions (root, leaf, stem and their mixture) were used for inulin extraction using aqueous extraction, enzyme-assisted extraction and enzyme-assisted decanter extraction. The extracts were used as carbon sources in submerged (SmF) fermentation, whereas the remaining solid residues were used as substrates in solid-state (SSF) fermentation for inulinase and FOS production by Aspergillus niger. The highest inulin concentration was obtained from the root extract produced by aqueous extraction at a 1:5 (w/v) ratio (197.55 g/L). The highest fructooligosaccharides (FOS) concentration (12.64 g/L) was achieved by root enzyme-assisted extraction. In SmF, the highest total FOS concentration (13.99 g/L) was obtained from leaf enzyme-assisted extracts (LEE), whereas in SSF, the maximum total FOS concentration (20.80 g/L) was achieved using leaf enzyme-assisted decanter extracts. Inulinase from the aqueous root extracts was 609.72 U/mL during SmF and the leaf enzyme-assisted decanter extracts was 450.86 U/mL during SSF. Inulin extraction from fractions of Stevia, high inulinase production in Smf, and enhanced FOS production from residual biomass in SSF enable the generation of multiple value-added products and support circular economy and biorefinery principles.
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(This article belongs to the Special Issue Process Intensification in Microbial Biotechnology for Fermentation)
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Open AccessReview
Microorganisms in Fermented Foods and Their Contribution to Oral Health: A Narrative Review
by
Georgios Chrysochoou, Socratis Thomaidis, Maria Antoniadou and Theodoros Varzakas
Fermentation 2026, 12(8), 376; https://doi.org/10.3390/fermentation12080376 - 9 Aug 2026
Abstract
Fermented foods contain diverse microorganisms that may influence oral microbial ecology and contribute to oral health. This narrative review aimed to examine the current evidence regarding microorganisms present in fermented foods and their role in oral health promotion. Twenty relevant clinical, experimental, observational,
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Fermented foods contain diverse microorganisms that may influence oral microbial ecology and contribute to oral health. This narrative review aimed to examine the current evidence regarding microorganisms present in fermented foods and their role in oral health promotion. Twenty relevant clinical, experimental, observational, and review studies published between 2016 and 2026 were identified through a structured literature search and critically synthesized according to major thematic areas, including oral microbiota modulation, dental caries prevention, periodontal health, probiotic delivery systems, and evidence from review articles. The findings appear to indicate that fermented food-derived microorganisms may reduce cariogenic and periodontal pathogens, modulate oral biofilms, improve gingival health, and promote microbial homeostasis. Yogurt, kefir, fermented dairy products, and kimchi-derived microorganisms were the most frequently investigated sources. Emerging evidence further suggests that the benefits of these microorganisms are linked to ecological regulation of the oral microbiome and host–microbe interactions rather than direct antimicrobial activity alone. Microorganisms associated with fermented foods may therefore represent promising functional dietary components for supporting oral health and complementing preventive oral healthcare strategies. Further well-designed clinical studies are needed to establish strain-specific recommendations and explore the potential of postbiotic applications in dentistry.
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(This article belongs to the Special Issue Microbial Ecosystems in Fermented Foods)
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Open AccessArticle
Bioaugmentation Anaerobic Digestion of Huangshui by Saccharomyces cerevisiae
by
Xiaoying Zhang, Yujie Zhang, Zhaoyi Duan, Zhouying Liu, Bowen Xu, Ruixi Wang and Jishi Zhang
Fermentation 2026, 12(8), 375; https://doi.org/10.3390/fermentation12080375 - 9 Aug 2026
Abstract
Huangshui (HS) is rich in organic matter from Baijiu production. Anaerobic digestion (AD) of HS offers a hopeful strategy for efficient waste utilization and energy recovery. However, high organic load often causes low methane (CH4) yield and process instability. The aim
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Huangshui (HS) is rich in organic matter from Baijiu production. Anaerobic digestion (AD) of HS offers a hopeful strategy for efficient waste utilization and energy recovery. However, high organic load often causes low methane (CH4) yield and process instability. The aim of this study was to explore the mechanism of Saccharomyces cerevisiae (S. cerevisiae) to favor AD systems for more CH4. And the results revealed that with an inoculation at 8% (v/v) addition of S. cerevisiae, CH4 yield was 219.7 mL/g COD, representing a 20% increase compared to the control without S. cerevisiae. The main manifestation of augmentation was the increased bioavailability of dissolved organic compounds. In addition, the ethanol produced by S. cerevisiae under anaerobic conditions served as an electron donor to supply metabolic intermediates supporting methanogenesis, enriching Bacteroidetes_vadinHA17, Longilinea and Methanosaeta. This strategy promoted organic matter degradation and increased CH4 yield, favoring the efforts of the industry to reduce pollution and carbon emissions.
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(This article belongs to the Section Industrial Fermentation)
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Open AccessArticle
Effects of Co-Fermentation with Saccharomyces cerevisiae and Lactobacillus plantarum on Volatile Flavor Compounds and Texture Properties of Duck Meat
by
Sijia Chen, Lei Dong, Jia Guo, Runmei Zhang and Jun Cai
Fermentation 2026, 12(8), 374; https://doi.org/10.3390/fermentation12080374 - 9 Aug 2026
Abstract
This study investigated the changes in flavor and texture of duck meat during co-fermentation with Saccharomyces cerevisiae and Lactobacillus plantarum. Characteristic flavor compounds of duck meat, including hexanal, nonanal, octanal, (E)-2-octenal, and 1-octen-3-ol, were detected. With the extension of fermentation time, the
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This study investigated the changes in flavor and texture of duck meat during co-fermentation with Saccharomyces cerevisiae and Lactobacillus plantarum. Characteristic flavor compounds of duck meat, including hexanal, nonanal, octanal, (E)-2-octenal, and 1-octen-3-ol, were detected. With the extension of fermentation time, the variety and contents of alcohols, ketones, and esters increased. The contents of 1-hexanol, ethanol and 3-hydroxy-2-butanone increased from 0.24 ng/g, 0 ng/g and 0 ng/g prior to fermentation to 11.25 ng/g, 8.93 ng/g and 1.94 ng/g after fermentation. Respectively these compounds endow duck meat with intense fruity, alcoholic and creamy aromas. Meanwhile, the contents of compounds that produce a peculiar smell in duck meat, such as hexanal, heptanal, pentanal, and 1-octen-3-ol, decreased significantly. After fermentation, the hexanal content declined from 11.2 ng/g (before fermentation) to 2.7 ng/g, and the 1-octen-3-ol content decreased from 2.21 ng/g to 1.51 ng/g. Furthermore, under the interaction of S. cerevisiae and L. plantarum, the hardness of duck meat decreased from 1186.44 N to 561.48 N, and the chewiness decreased from 633.63 g to 167.16 g. These results demonstrate that co-fermentation with S. cerevisiae and L. plantarum is an effective approach to improve the flavor and quality of duck meat. This study provides a new strategy for the application of microbial fermentation in processing duck meat to enhance its flavor and quality.
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(This article belongs to the Section Fermentation for Food and Beverages)
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