Yeast Biotechnology

A topical collection in Fermentation (ISSN 2311-5637). This collection belongs to the section "Microbial Metabolism, Physiology & Genetics".

Viewed by 2509

Editor


E-Mail Website
Collection Editor
Structural Biology Brussels Lab, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, 1050 Brussels, Belgium
Interests: yeast biotechnology; cell immobilization; beer brewing biochemistry and fermentation; mini- and microbioreactors; Saccharomyces cerevisiae; Candida; yeast space biology (bioreactors for microgravity research); yeast adhesins; yeast systems biology; glycobiology; nanobiotechnology; Atomic Force Microscopy; protein crystallization; yeast protein structural biology
Special Issues, Collections and Topics in MDPI journals

Topical Collection Information

Dear Colleagues,

Yeasts are truly fascinating microorganisms. Due to their diverse and dynamic activities, they have been used to produce many interesting products, such as beer, wine, bread, biofuels and biopharmaceuticals. Saccharomyces cerevisiae (bakers’ yeast) is likely the most human-exploited yeast species. Saccharomyces is a popular choice for industrial applications, although its use in beer production dates to at least the sixth millennium BC. Bakers’ yeast represents a cornerstone of modern biotechnology, enabling the development of efficient production processes for antibiotics, biopharmaceuticals, technical enzymes, and ethanol and biofuels.

Today, diverse yeast species are explored for industrial applications, such as, e.g., the Saccharomyces species, Pichia pastoris and other Pichia species, Kluyveromyces marxianus, Hansenula polymorpha, Yarrowia lipolytica, Candida species, Phaffia rhodozyma, wild yeasts for beer brewing and winemaking, and others with proven potential.

Yeast cells can also be benign for humans, since some yeast species can be pathogenic and cause infections, especially in individuals with weakened immune systems. Strategies to fight pathogenic yeasts have been developed through recent endeavors, such as the development of synthetic antifungal agents, the use of natural products with antifungal activity, and the use of yeasts with antimicrobial infections. Other strategies such as biocontrol agents, reactive oxygen species and RNA interference have been developed to combat pathogenic yeasts. These approaches aim to inhibit the growth and survival of pathogenic yeasts, thereby preventing and treating yeast infections.

This Topical Collection, “Yeast Biotechnology”, is a continuation of the Special Issues “Yeast Biotechnology” series of Fermentation (published by MDPI). This installment will compile the current state-of-the-art research and technology in the area of yeast biotechnology, and highlight the prominent research directions for hot topics, such as recently developed techniques for characterizing yeast and their physiology (including omics and nanobiotechnology techniques), methods for adapting industrial strains (including metabolic, synthetic and evolutionary engineering) and the use of yeasts as microbial cell factories to produce biopharmaceuticals, enzymes, alcohols, organic acids, flavors and fine chemicals, advances in yeast fermentation technology and industrial fermentation processes, as well as yeast biotechnology strategies in fighting pathogenic yeasts.

Topics of interest include, but are not limited to:

Yeast characterization and analysis:
Brewing yeasts (including wild yeasts), wine yeasts and baker’s yeasts;
Evolution and variation in industrial yeast genomes;
Yeast systems biology: genomics, proteomics, fluxomics, metabolomics, and omics integration;
Yeast nanobiotechnology (nano-analysis techniques, construction of nanostructures, etc.).

Yeast strain engineering:
Yeast metabolic engineering: production of biofuels, secondary metabolites, commodity chemicals, proteins, biopharmaceuticals and material precursors;
Yeast synthetic biology: yeasts as cell factories, tools for controlling enzyme expression levels, strategies for regulating spatial localization of enzymes in yeast, regulatory networks and biomolecular logic gates;
Strain improvement via evolutionary engineering.

Fermentation technology:
Industrial bioreactors;
Mini- and micro-bioreactors: single-cell analysis, high-throughput screening and microfluidic bioreactors;
Process intensification: high-density fermentations, high-gravity fermentation, yeast cell immobilization;
Fermentative stress adaptation.

Industrial fermentation processes:
Production of food (bread, etc.) and beverages (beer, wine, cider, etc.);
Production of bakers’ yeast;
Production of biofuels (bioethanol, 1-butanol, biodiesel, jetfuels), commodity chemicals, pharmaceuticals, material precursors, and secondary metabolites.

Fight against pathogenic yeasts:
Antifungal agents, yeast killer toxins, probiotic yeasts, and biocontrol agent development and assessment.

Prof. Dr. Ronnie Willaert
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • systems biology
  • genomics
  • proteomics
  • fluxomics
  • metabolomics
  • synthetic yeast biology
  • metabolic engineering
  • evolutionary engineering
  • industrial yeast products
  • beer
  • wine
  • bread
  • biofuels
  • commodity chemical
  • biopharmaceuticals
  • material precursors
  • yeast fermentation technology
  • industrial bioreactors
  • mini- and microbioreactors
  • high-density fermentations
  • yeast stress adaptation
  • yeast biotechnology to fight pathogenic yeasts

Related Special Issues

Published Papers (2 papers)

2024

24 pages, 4348 KiB  
Article
Bioremediation with an Alkali-Tolerant Yeast of Wastewater (Nejayote) Derived from the Nixtamalization of Maize
by Luis Carlos Román-Escobedo, Eliseo Cristiani-Urbina and Liliana Morales-Barrera
Fermentation 2024, 10(4), 219; https://doi.org/10.3390/fermentation10040219 - 17 Apr 2024
Viewed by 913
Abstract
Nejayote, the wastewater from the nixtamalization of maize, is difficult to biodegrade due to its abundant calcium content; low levels of nitrogen, phosphorus, and easily assimilable sugars; elevated pH; and high chemical oxygen demand (COD). The aim of the present study was to [...] Read more.
Nejayote, the wastewater from the nixtamalization of maize, is difficult to biodegrade due to its abundant calcium content; low levels of nitrogen, phosphorus, and easily assimilable sugars; elevated pH; and high chemical oxygen demand (COD). The aim of the present study was to isolate microorganisms capable of utilizing filtered nejayote (NEM) as the only source of carbon for growth and to test the best microorganism for the bioremediation of this wastewater by lowering the level of pH and COD. Of the 15 strains of microorganisms tested, Rhodotorula mucilaginosa LCRE was chosen and identified using molecular techniques. Subsequently, its growth kinetics were characterized during cultivation in unenriched NEM (control) and NEM enriched with nitrogen and phosphorus salts. R. mucilaginosa LCRE showed a greater growth (6.9 ≤ X ≤ 8.9 g L−1), biomass yield (0.33 ≤ YX/S ≤ 0.39 g g−1), and specific growth rate (0.748 ≤ µ ≤ 0.80 day−1) in the enriched versus control NEM (X = 6.55 g L−1, YX/S = 0.28 g g−1, and µ = 0.59 day−1). However, a higher total sugar consumption (94.98%), better COD removal efficiency (75.5%), and greater overall COD removal rate (1.73 g L−1 h−1) were found in the control NEM. Hence, R. mucilaginosa LCRE holds promise for the efficient bioremediation of nejayote without costly pretreatments or nutrient supplementation. Full article
Show Figures

Figure 1

15 pages, 3862 KiB  
Article
Co-Inoculation of Latilactobacillus sakei with Pichia kluyveri or Saccharomyces boulardii Improves Flavour Compound Profiles of Salt-Free Fermented Wheat Gluten
by Shuoyu Chen, Fanxin Zhang, Edwin Ananta, Jeroen André Muller, Youyun Liang, Yuan Kun Lee and Shao-Quan Liu
Fermentation 2024, 10(2), 75; https://doi.org/10.3390/fermentation10020075 - 24 Jan 2024
Viewed by 1229
Abstract
A wheat gluten fermentation process with the inoculation of different microorganisms under salt-free conditions has the potential to produce varying flavour profiles. As research on the co-fermentation of yeasts and lactic acid bacteria (LAB) in salt-free wheat gluten fermentation is scarce, the current [...] Read more.
A wheat gluten fermentation process with the inoculation of different microorganisms under salt-free conditions has the potential to produce varying flavour profiles. As research on the co-fermentation of yeasts and lactic acid bacteria (LAB) in salt-free wheat gluten fermentation is scarce, the current work studied the flavour impact on fermented wheat gluten by the co-inoculation of Latilactobacillus sakei with one yeast (Saccharomyces boulardii or Pichia kluyveri). The results showed that similar glucose and organic acid levels were detected, but early death of yeasts was observed during liquid-state fermentation (LSF) in co-fermentations. The concentrations of most free amino acids were comparable. Volatile compound analysis showed synergistic effects in co-cultured fermentations on the production of certain compounds such as isoamyl acetate. Principal component analysis revealed clear differences in volatile profiles between co-fermentation and single-strain fermentation. Therefore, a fermented sauce produced by co-inoculating LAB and yeast with a new and fruitier flavour was developed. Full article
Show Figures

Figure 1

Back to TopTop