Application of Lactobacillus in Fermented Food and Beverages, 2nd Edition

A Special Issue of Fermentation (ISSN 2311-5637) belonging to the section "Fermentation for Food and Beverages".

Deadline for manuscript submissions: closed (28 February 2026) | Viewed by 9642

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Guest Editor
Department of Health Sciences, Universidad de Jaén, Jaen, Spain
Interests: molecular biological techniques; PCR; antibiotic resistance; microbiology techniques electrophoresis
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Special Issue Information

Dear Colleagues,

Fermented foods have been integral to human culture since antiquity, with a worldwide importance in human nutrition, health, and economy. Within this context, Lactobacillus strains have had and still have a very important role.

The genus Lactobacillus includes Gram-positive, fermentative, facultatively anaerobic and non-spore-forming microorganisms. They are commonly employed in food fermentation to increase shelf-life, decrease hygienic risks, increase quality and functionality and improve the taste and flavor of foods. Furthermore, they have been shown to promote human health as probiotic strains.

Most fermented foods are based on tradition and artisanal fermentation processes, where Lactobacillus strains may promote spontaneous fermentations as autochthonous food microorganisms or may be added as starters in controlled fermentations to develop new foods or characteristics. In this sense, the food matrix and the strain will determine the fermentative behavior and therefore the final product.

All manuscripts that fall under these specific topics are welcome. Manuscripts dealing with the understanding of the interaction of Lactobacillus in fermented foods and beverages, in order to determine its role in the manufacturing practices, the creation of new foods, food safety, and health, are particularly welcome.

Dr. Leyre Lavilla-Lerma
Guest Editor

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Keywords

  • Lactobacillus
  • fermented food
  • fermentation quality
  • starter culture
  • food safety
  • probiotic
  • health
  • microbiota
  • omics analysis

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Published Papers (3 papers)

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Research

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15 pages, 647 KB  
Article
Biotechnological Characterization and Safety Assessment of Lacticaseibacillus paracasei and Levilactobacillus brevis Strains Carrying entAS-48 and entQ Genes
by Roumaissaa Belkacem, Qada Benameur, Smaranda Crăciun, Hajer Kilani, Claudio Gervasi, George Cosmin Nadăș, Adriana Györke, Mohamed Salah Abbassi and Teresa Gervasi
Fermentation 2026, 12(1), 12; https://doi.org/10.3390/fermentation12010012 - 24 Dec 2025
Cited by 1 | Viewed by 2004
Abstract
In our previous work, we reported for the first time the presence of enterocin-encoding genes in novel Lacticaseibacillus paracasei (L. paracasei) and Levilactobacillus brevis (Lev. brevis) strains isolated from artisanal dairy products made from raw cow milk. The aim [...] Read more.
In our previous work, we reported for the first time the presence of enterocin-encoding genes in novel Lacticaseibacillus paracasei (L. paracasei) and Levilactobacillus brevis (Lev. brevis) strains isolated from artisanal dairy products made from raw cow milk. The aim of this study was to isolate enterocin-positive lactic acid bacteria (LAB) from artisanal dairy products and assess their technological characteristics and safety for potential application in food systems. LAB isolates were characterized using phenotypic tests, Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) identification, and PCR detection of enterocin genes, followed by evaluation of their physiological and technological properties and a comprehensive safety assessment, including antimicrobial resistance, virulence, biogenic amine, and integron genes. Two strains, L. paracasei S2 and Lev. brevis S62, carried enterocin genes (entAS-48 and entQ) and exhibited strong acidifying and proteolytic activities, along with antibacterial effects against foodborne pathogens and reference strains. Both isolates tolerated environmental stresses, including low pH, and lacked virulence factors, clinically relevant antibiotic resistance genes, biogenic amine production, and integron elements. These results indicate that the strains are safe, multifunctional, and suitable for developing regionally adapted dairy products, highlighting artisanal dairy products as a valuable source of novel LAB with promising biotechnological applications. Full article
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15 pages, 1323 KB  
Article
Functional Kimchi Beverage Enhanced with γ-Aminobutyric Acid (GABA) Through Serial Co-Fermentation Using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020
by Min-Jeong Kwon, Ji-Eun Kim and Sam-Pin Lee
Fermentation 2025, 11(1), 44; https://doi.org/10.3390/fermentation11010044 - 19 Jan 2025
Cited by 6 | Viewed by 5204
Abstract
A plant-based beverage enhanced with GABA was developed through serial co-fermentation using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020. The first lactic acid fermentation was performed by Leu. citreum S5 with a vegetable mixture consisting of sliced radish, ginger, garlic, red pepper, bell [...] Read more.
A plant-based beverage enhanced with GABA was developed through serial co-fermentation using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020. The first lactic acid fermentation was performed by Leu. citreum S5 with a vegetable mixture consisting of sliced radish, ginger, garlic, red pepper, bell pepper, and sucrose. The viable cell count of Leu. citreum S5 increased to 9.11–9.42 log CFU/mL with higher sucrose contents, indicating the highest value of 9.42 log CFU/mL at 5% sucrose on day 1. Mannitol and dextran production levels in the first fermented vegetable mixture were 6.66–14.54 mg/mL and 0.44–2.26%, respectively. A higher sucrose content produced more dextran, resulting in a concomitant increase in viscosity of 49.4 mPa·s. The second co-fermentation for the kimchi beverage base was performed by Lb. plantarum KS2020 for 5 days, resulting in 8.22–9.60 log CFU/mL. The pH of the co-fermented kimchi beverage base increased to 6.19–9.57 with an increasing monosodium glutamate (MSG) content (3–7%), while titratable acidity significantly decreased to 0.0–0.8%. The final co-fermented kimchi beverage base was enriched with 2.6% GABA. Consequently, a GABA kimchi beverage base with probiotics, a red pigment, and a pleasant flavor was developed using only vegetable ingredients by serial co-fermentation using lactic acid bacteria. Full article
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Review

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16 pages, 449 KB  
Review
Applications of Food-Associated Lactobacillaceae in Fermented Foods, Health, and Emerging Biotechnologies
by Shazia Pathan, Veronika Karlegan and David Q. Shih
Fermentation 2026, 12(2), 75; https://doi.org/10.3390/fermentation12020075 - 30 Jan 2026
Cited by 2 | Viewed by 1793
Abstract
The family Lactobacillaceae, reclassified in 2020 into 25 genera comprising 261 species, remains one of the most extensively studied groups of lactic acid bacteria (LAB) due to its wide distribution in fermented products, commensal presence in the gastrointestinal tract, and studied health [...] Read more.
The family Lactobacillaceae, reclassified in 2020 into 25 genera comprising 261 species, remains one of the most extensively studied groups of lactic acid bacteria (LAB) due to its wide distribution in fermented products, commensal presence in the gastrointestinal tract, and studied health effects. Long classified as “generally recognized as safe (GRAS)” by the U.S. Food and Drug Administration (FDA), these organisms not only contribute to the flavor, texture, and preservation of fermented foods and beverages but also provide important health benefits as probiotics. Their metabolic versatility allows them to produce lactic acid, bacteriocins, and other bioactive compounds that inhibit pathogenic microorganisms and enhance food quality. This review provides a comprehensive overview of the functional roles of members of the Lactobacillaceae family in the context of the food matrix in fermentation, health, and biotechnology, and examines recent advances in functional genomics, metabolomics, and extracellular vesicle research to highlight future directions for leveraging these microorganisms in sustainable and innovative applications. Full article
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