Fermented Cereals and Legumes: Innovation for the Development and Characterization of Functional Foods

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation for Food and Beverages".

Deadline for manuscript submissions: 31 May 2026 | Viewed by 3143

Special Issue Editor


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Guest Editor
National Scientific and Technical Research Council, Argentina and National University of Littoral, Santa Fe, Argentina
Interests: plant-based foods; fermented beverages; phenolic compounds; food bioactivity; bioaccessibility; bioavailability of bioactives

Special Issue Information

Dear Colleagues,

Fermentation of cereals and legumes has emerged as a versatile biotechnological strategy to enhance the nutritional, sensory, and functional qualities of plant-based foods. These substrates provide rich carbon and nitrogen sources that sustain diverse microbial communities, including lactic acid bacteria, yeasts, and other beneficial microorganisms. Through their metabolic activity, these microbes degrade antinutritional factors such as phytic acid, tannins, and enzyme inhibitors, while improving the bioavailability of minerals and amino acids. Fermentation also promotes the release and transformation of bioactive compounds with antioxidant, anti-inflammatory, and gut-modulatory properties, contributing to better metabolic and intestinal health. A wide range of fermented products, including bread, snacks, sauces, and beverages derived from cereals and legumes, represents promising vehicles for developing sustainable, functional foods. This Special Issue aims to advance understanding of the microbial functionality and biochemical mechanisms driving cereal- and legume-based fermentations, and to explore their implications for nutritional quality, product innovation, and human well-being.

Papers dealing with or working on the following topics are invited to publish the following:

  • Characterization of lactic acid bacteria, yeasts, or mixed cultures in cereal/legume fermentations;
  • Nutritional and functional enhancement through fermentation (proteins, phenolic compounds, bioactive peptides);
  • Reduction of antinutritional factors;
  • Bioavailability and bioaccessibility studies after fermentation;
  • Omics approaches to study fermentation microbiota and metabolites;
  • Development of novel fermented cereal- and legume-based foods.
  • Sustainability and circular bioeconomy perspectives in fermented plant-based systems.

Dr. Franco Van de Velde
Guest Editor

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Keywords

  • cereal fermentation
  • legume fermentation
  • lactic acid bacteria
  • phenolic compounds
  • bioaccessibility
  • sustainable food systems
  • circular bioeconomy
  • plant-based beverages
  • functional foods
  • product innovation

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Published Papers (2 papers)

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Research

20 pages, 1929 KB  
Article
Fermentation Unlocks the Functional Role of Amaranth in Modulating Wheat/Amaranth Sourdough Microbiota and Inhibiting Yeast Growth of Refrigerated Doughs
by Carolina Dardis, Emiliano Bilbao, María Cristina Añón and Analía G. Abraham
Fermentation 2026, 12(2), 80; https://doi.org/10.3390/fermentation12020080 - 2 Feb 2026
Viewed by 1613
Abstract
This study focuses on the development of refrigerated doughs without chemical preservatives to obtain a clean-label product. Sourdough-based strategies were applied to replace conventional preservatives, using both spontaneous flour fermentation and a defined starter culture of Lactiplantibacillus plantarum CIDCA 8327. In parallel, a [...] Read more.
This study focuses on the development of refrigerated doughs without chemical preservatives to obtain a clean-label product. Sourdough-based strategies were applied to replace conventional preservatives, using both spontaneous flour fermentation and a defined starter culture of Lactiplantibacillus plantarum CIDCA 8327. In parallel, a partial substitution of wheat flour with 7% amaranth flour was evaluated. To monitor fermentation, pH, titratable acidity, and viable microorganism counts were determined in the sourdoughs, along with culture-independent analyses of microbial communities in two independent spontaneously fermented trials. Dough discs prepared from these sourdoughs were analyzed for pH, titratable acidity, and viable microorganisms, and shelf life was determined based on the appearance of visible mould during refrigerated storage. No substantial differences were observed in the physicochemical parameters of the sourdoughs; however, significant differences in microbial communities were detected, influenced by both amaranth addition and wheat flour batch variability. Dough discs prepared with amaranth flour and spontaneous fermentation showed an extended shelf life and lower mould and yeast counts during refrigerated storage. The use of the starter increased shelf life compared to non-fermented doughs but was less effective than spontaneous sourdough with amaranth. Overall, these results highlight the potential of sourdough technology and amaranth flour for developing clean-label refrigerated products. Full article
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22 pages, 1002 KB  
Article
Effect of Fermentation on Phytochemical, Antioxidant, Functional, and Pasting Properties of Selected Legume Flours
by Janet Adeyinka Adebo
Fermentation 2026, 12(1), 62; https://doi.org/10.3390/fermentation12010062 - 21 Jan 2026
Cited by 1 | Viewed by 1079
Abstract
This study investigated the effect of fermentation time (24 and 48 h) on the pH, titratable acidity (TTA), phytochemicals, antioxidants, phenolic compounds, colour, functional, pasting, and thermal properties of flours from selected legumes (mung beans, haricot beans, butter beans, and black beans). The [...] Read more.
This study investigated the effect of fermentation time (24 and 48 h) on the pH, titratable acidity (TTA), phytochemicals, antioxidants, phenolic compounds, colour, functional, pasting, and thermal properties of flours from selected legumes (mung beans, haricot beans, butter beans, and black beans). The pH dropped significantly (p ≤ 0.05) after 48 h (6.61–4.91) of fermentation, with a corresponding increase in TTA, which ranged from 0.3 to 1.28 g lactic acid/100 g sample. Colour analysis showed that fermentation caused a decrease in L* values (2.97–23.86% reduction), with the highest reduction observed in black bean flour (23.86% at 24 h), along with an increase in the browning index. The total phenolic content increased significantly (p ≤ 0.05) in all the samples, with the most pronounced increase observed in mung bean 24 h (6.85 mg GAE/g). Similarly, the values for total flavonoid increased from 2.26 to 6.48 mg QE/g, and antioxidant activities such as DPPH ranged from 45.04 to 74.51%, FRAP from 1.65 to 8.03 Mm TE/g, and ABTS from 60.86 to 90.01%. Ultra-high performance liquid chromatography–photodiode array quantification of the targeted phenolic compounds showed a significant increase, with the highest notable increase for trans-ferulic acid in mung bean (330% after 48 h). Water absorption capacity generally showed an increase, whereas bulk density ranged from 0.55 to 0.91 g/cm3 and decreased in all legumes. There were differences in the pasting properties of the selected legumes. The peak time of unfermented butter bean was 33.08 min and remained constant at 33.15 min at 24 and 48 h of fermentation. Thermal analysis indicated the alteration of gelatinization parameters, with a decrease in peak temperature, whereas higher gelatinization enthalpy was observed. Findings from this study show that fermentation with the starter cultures can significantly improve the bioactive compound and functional properties of legume flours and thus act as potential ingredients in functional food development. Full article
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