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Role of Intercropping, Herbicides and Fungicides in Compensating for the Lack of Crop Rotation in Long-Term Continuous Cropping of Two Potato Cultivars -
Polyploidy Promotes Larger Mango Fruits with Cultivar-Specific Quality Changes -
An Overview of Bacterial Canker in Stone Fruits Caused by Different Pseudomonads: Pseudomonas syringae Species Complex and Related Species
Journal Description
Agriculture
Agriculture
is an international, peer-reviewed, open access journal published semimonthly online.
- Open Access— free for readers, with article processing charges (APC) paid by authors or their institutions.
- High Visibility: indexed within Scopus, SCIE (Web of Science), GEOBASE, PubAg, AGRIS, RePEc, and other databases.
- Journal Rank: JCR - Q1 (Agronomy) / CiteScore - Q1 (Plant Science)
- Rapid Publication: manuscripts are peer-reviewed and a first decision is provided to authors approximately 17.4 days after submission; acceptance to publication is undertaken in 2.4 days (median values for papers published in this journal in the first half of 2026).
- Recognition of Reviewers: Reviewers whose reports are timely and of high quality receive an APC discount voucher for a future publication in an MDPI journal. Become a reviewer.
- Companion journals for Agriculture include: Poultry, Grasses, Crops, AIPA and Grain Science.
- Journal Cluster of Agricultural Science: Agriculture, Agronomy, Horticulturae, Soil Systems, AgriEngineering, Crops, Seeds, Grasses, Agrochemicals and AI and Precision Agriculture.
Impact Factor:
4.5 (2025);
5-Year Impact Factor:
4.6 (2025)
Latest Articles
Slip-Ratio-Aware Energy Management of a Hybrid Tractor Under Variable Plowing Loads Using a DP-Calibrated ECMS
Agriculture 2026, 16(18), 2000; https://doi.org/10.3390/agriculture16182000 (registering DOI) - 17 Sep 2026
Abstract
To enhance the fuel economy and operational adaptability of hybrid tractors under variable plowing loads, this paper proposes a slip-ratio-aware equivalent consumption minimization strategy (ECMS) calibrated via dynamic programming (DP). A resistance–slip ratio prediction model was first identified using plowing resistance and slip
[...] Read more.
To enhance the fuel economy and operational adaptability of hybrid tractors under variable plowing loads, this paper proposes a slip-ratio-aware equivalent consumption minimization strategy (ECMS) calibrated via dynamic programming (DP). A resistance–slip ratio prediction model was first identified using plowing resistance and slip ratio data collected from soil-bin tests. The predicted slip ratio was integrated into the demand power model to quantify slip-induced traction losses. Offline DP was subsequently applied to generate globally optimized power split trajectories and establish a baseline equivalence-factor map indexed by plowing resistance level and battery state of charge (SOC). For real-time operation, the equivalence factor is dynamically adjusted via SOC feedback and normalized slip ratio deviation, enabling coordinated power distribution among the engine, MG1, and MG2. Powertrain bench tests were conducted by reproducing variable plowing loads using a dynamometer. The equivalent plowing resistance was calculated from measured load torque, and the corresponding slip ratio was estimated using the identified prediction model. Compared with A-ECMS, the proposed strategy reduced equivalent fuel consumption by 14.02% in simulation and 7.33% in bench tests. The proportion of engine operation in the high-efficiency region increased from 61% to 80% in simulation and from 65% to 77% in the bench test, while the corresponding proportion for the electric motors increased from 87% to 92% and from 88% to 90%, respectively. The SOC deviation decreased from 3.03% to 2.26% in simulation and from 3.07% to 2.43% in the bench test. These results demonstrate that the proposed strategy improves fuel economy, SOC regulation, and component operating efficiency under variable plowing loads.
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(This article belongs to the Section Agricultural Technology)
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A Method for Constructing Farmland Semantic Point Cloud Maps Based on LiDAR–Camera Fusion
by
Mingxuan Cheng, Shiwei Ma, Fuwei Li, Jianguo Zhao and Zhikai Ma
Agriculture 2026, 16(18), 1999; https://doi.org/10.3390/agriculture16181999 (registering DOI) - 17 Sep 2026
Abstract
With the development of intelligent agricultural machinery and unmanned farms, agricultural production is gradually evolving toward greater autonomy, reduced labor dependence, and higher precision. To address the limitation of a single sensor in unstructured farmland environments, where three-dimensional metric information and semantic information
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With the development of intelligent agricultural machinery and unmanned farms, agricultural production is gradually evolving toward greater autonomy, reduced labor dependence, and higher precision. To address the limitation of a single sensor in unstructured farmland environments, where three-dimensional metric information and semantic information cannot be captured effectively at the same time, this study proposes a method for constructing a farmland semantic point cloud map based on LiDAR–camera fusion. DeepLabV3+ was used to extract pixel-level semantic information, while ground-plane alignment, normalized projection, effective field-of-view constraints, and spatiotemporal synchronization were integrated to map two-dimensional labels onto three-dimensional point clouds. The proposed method was validated in three plots with different boundary shapes using RTK-GNSS reference boundaries. The experimental results showed that the DeepLabV3+ model achieved an mPA of 90.71% and an mIoU of 82.86% on the test set. The constructed semantic map yielded an overall mean point-position error of 0.192 m and an RMSE of 0.216 m, with an overall area coverage of 97.20%. The proposed method therefore enables three-dimensional semantic labeling and farmland boundary reconstruction while providing metric-scale semantic information for environmental perception and autonomous navigation of intelligent agricultural machinery.
Full article
(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
Open AccessArticle
Effects of Different Chemical Thinning Strategies on Yield Performance, Fruit Quality, and Multivariate Responses in Intensive Apple Production
by
Ádám Csihon, Marianna Sipos, Tamás Szentpéteri and Imre J. Holb
Agriculture 2026, 16(18), 1998; https://doi.org/10.3390/agriculture16181998 (registering DOI) - 17 Sep 2026
Abstract
Optimizing crop load through chemical thinning is essential for yield stability and fruit quality in intensive apple production, yet the relationships between tree growth, yield components, and fruit quality remain insufficiently characterized. This three-year study (2022–2024) evaluated four thinning strategies (control, ammonium thiosulfate
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Optimizing crop load through chemical thinning is essential for yield stability and fruit quality in intensive apple production, yet the relationships between tree growth, yield components, and fruit quality remain insufficiently characterized. This three-year study (2022–2024) evaluated four thinning strategies (control, ammonium thiosulfate [ATS]+ethephon, benzyladenine [BA]+ethephon, and ATS+BA+ethephon) across parameters of the apple cv. ‘Gala Schniga Schnitzer’: trunk cross-sectional area (TCSA), fruit yield (Y), fruit number per tree (FNT), crop load-number (CLnm), crop load-kg (CLkg), fruit size (FS), fruit weight (FW), water-soluble solids content (SSC), and fruit firmness (FF). Chemical thinning and year significantly but differentially affected yield components and fruit quality traits. In the ATS+BA+ethephon treatment, FS in 2023, FW in 2023 and 2024, and Y in 2024 significantly exceeded the control treatment in the respective years. Correlation analysis revealed strong positive relationships between FNT–Y, CLnm–CLkg, and FW–FS, with the strongest association observed between FW and FS (r = 0.98 in 2022 and 2023). Regression analyses confirmed significant linear relationships between FW–FS, FNT–Y, and CLnm–CLkg across thinning treatments. PCA explained 66.93, 54.89, and 50.91% of total variance by PC1 and PC2 in 2022–2024, respectively, with FS and FW showing the longest vectors in all years. The PCA structure varied among years, reflecting differences in the relative contributions of yield, crop load, tree growth, and fruit quality traits. Overall, chemical thinning should be considered not merely for reducing fruit number, but for regulating crop load and balancing productivity and fruit development, with effects varying by trait and year.
Full article
(This article belongs to the Special Issue Integrated Farm Management Strategies for Sustainable Production of Horticultural Fruit Crops)
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Open AccessArticle
Decoupled Foundation Models for Instance Segmentation and Automated Detection of Humidity-Induced Tomato Leaf Necrosis
by
Emmanouil Savvakis, María del Carmen Martínez-Ballesta, Dimitrios Kapetas and Eleftheria Maria Pechlivani
Agriculture 2026, 16(18), 1997; https://doi.org/10.3390/agriculture16181997 (registering DOI) - 17 Sep 2026
Abstract
Tomato cultivation is highly vulnerable to both biotic and abiotic stressors, which together constitute major constraints on global crop productivity and food security. Among these, abiotic stressors such as excessive humidity are particularly challenging because they often induce physiological disorders and necrotic leaf
[...] Read more.
Tomato cultivation is highly vulnerable to both biotic and abiotic stressors, which together constitute major constraints on global crop productivity and food security. Among these, abiotic stressors such as excessive humidity are particularly challenging because they often induce physiological disorders and necrotic leaf symptoms that resemble biological infections, complicating early diagnosis and timely intervention. Manual scouting is labor-intensive and prone to missed early-stage symptoms, motivating automated deep-learning-based detection systems. In this study, a multi-step AI pipeline for the automated segmentation and classification of humidity-induced necrotic leaf spots in tomatoes is proposed. A dataset of 218 RGB images was collected, yielding 3218 annotations across three classes (brown necrotic spots, yellow necrotic spots, and no necrotic leaves). Six end-to-end instance segmentation pipelines combining YOLO26m (for detection and segmentation), SAM2 (for zero-shot prompted segmentation), and either fine-tuned DINOv2 or EfficientNet-B3 (for downstream classification) were systematically designed and evaluated. Fine-tuned DINOv2 reached a macro F1-Score of 0.926 for per-instance crop classification, above EfficientNet-B3, ResNet-50 and Swin-Small baselines (0.886–0.901). The best-performing configuration (YOLO26m-det + SAM2 + DINOv2) achieved mAP@50 of 0.828, outperforming the single-model YOLO26m-seg baseline by approximately 8%. These results demonstrate that decoupling localization from classification through task-specific foundation models consistently outperforms single-model training on small, class-imbalanced agricultural datasets. By leveraging zero-shot segmentation foundation models like SAM2, this approach effectively bridges the gap in diagnostic performance for data-limited agricultural settings.
Full article
(This article belongs to the Special Issue Integrated Farm Management Strategies for Sustainable Production of Horticultural Fruit Crops)
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Open AccessArticle
Evaluating the Resilience of Cold-Region Rice Varieties to Climate Variability and Change Using the BDH-Rice Model: A Case Study in the Sanjiang Plain
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Mengqi Fu, Liping Feng, Huiqing Bai, Tao Li, Daquan Zhou, Shang Chen, Wenran Yu, Yabo Sun, Nan Chai, Mengya Li, Qiang Zhao, Lixin Qiu and Xianguan Chen
Agriculture 2026, 16(18), 1996; https://doi.org/10.3390/agriculture16181996 - 17 Sep 2026
Abstract
The Sanjiang Plain is a key cold-region rice production area in Northeast China, where climate variability and warming may alter the adaptability and yield stability of rice varieties. Identifying varieties with both high yield potential and stable performance under current and future climates
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The Sanjiang Plain is a key cold-region rice production area in Northeast China, where climate variability and warming may alter the adaptability and yield stability of rice varieties. Identifying varieties with both high yield potential and stable performance under current and future climates is therefore essential for climate-resilient rice production in this region. This study developed and evaluated a site-specific BDH-Rice-based framework for screening cold-region japonica rice varieties at the 856 Farm, a single representative production site in Heilongjiang Province. Multi-year field observations of 37 rice varieties from 2014 to 2023, together with local meteorological, soil, and management data, were used to localize variety-specific parameters. Seven parameters governing phenological development and yield formation were calibrated through a stepwise procedure. The localized model accurately simulated rice phenology, with RMSEs of 2.46 and 2.96 days for flowering and maturity, respectively (NRMSE < 3%), and reproduced grain yield with reasonable accuracy (NRMSE = 10.43%; index of agreement, D = 0.78). Simulated and observed yield rankings were significantly correlated in four of the seven testable year–dataset combinations (Spearman’s ρ = 0.782–0.933; Kendall’s τb = 0.600–0.833, p < 0.05). The high stability coefficient (HSC), which integrates yield level and interannual stability, was evaluated against field observations before its scenario application: simulated and observed HSC rankings were significantly correlated (ρ = 0.696, p < 0.001), indicating overall consistency in the stability ordering of the variety population, although agreement among the top-ranked varieties was only partial. Historical (1985–2020) and future (2030–2049) simulations identified twelve varieties with HSC > 1.0 in both periods. The future simulations were driven by a single global climate model (ACCESS-CM2) under the SSP2-4.5 scenario and are therefore conditional on this specific climate realization; the twelve candidates were proposed as site-specific, climate-conditional priority candidates requiring further field validation, with Longjing 63 leading in overall performance and Longjing 39 leading in future adaptability. These findings demonstrate that the localized BDH-Rice model can effectively support site-specific climate-adaptive variety screening at the 856 Farm and provides, in principle, a transferable methodological framework for prioritizing candidate varieties in comparable cold-region production systems of Northeast China. The cultivar rankings reported here were derived from a single-site calibration and have not yet been independently validated across multiple locations; multi-site evaluation is therefore the necessary next step before these single-site cultivar rankings can be generalized beyond the 856 Farm.
Full article
(This article belongs to the Special Issue Practical Use of Crop, Pest and Diseases Models in Sustainable Agriculture)
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Open AccessArticle
Cotton-Stalk Wood Vinegar Affects Nitrogen and Phosphorus Transformation in Chicken Manure Composting
by
Minghang Cheng, Jia Liu, Zhisheng Wang, Qian Liu, Shuo Li, Wenya Wang, Hui Yu and Hongmei Zhang
Agriculture 2026, 16(18), 1995; https://doi.org/10.3390/agriculture16181995 - 17 Sep 2026
Abstract
This study investigated the effects of cotton-stalk-derived wood vinegar produced at different pyrolysis temperatures on nitrogen and phosphorus transformations and the associated microbial mechanisms during the aerobic composting of chicken manure. Wood vinegar produced at 300, 350, 400, 450, and 500 °C was
[...] Read more.
This study investigated the effects of cotton-stalk-derived wood vinegar produced at different pyrolysis temperatures on nitrogen and phosphorus transformations and the associated microbial mechanisms during the aerobic composting of chicken manure. Wood vinegar produced at 300, 350, 400, 450, and 500 °C was applied in a 38-day composting experiment. Changes in physicochemical properties, nitrogen and phosphorus fractions, bacterial community succession, and potential functional profiles were analyzed using 16S rRNA gene sequencing, Spearman correlation analysis, and PICRUSt2-based COG functional prediction. The results showed that cotton-stalk-derived wood vinegar improved the composting process and regulated nutrient transformation. Compared with T0, the thermophilic phase was prolonged by 1–4 days in T1, T3, and T5. At the end of composting, the pH and electrical conductivity of all treatments ranged from 7.45 to 7.72 and from 1.37 to 1.75 mS/cm, respectively, which were within acceptable ranges for mature compost. The effects of wood vinegar on nitrogen and phosphorus transformations varied with pyrolysis temperature. All wood-vinegar-amended treatments maintained higher final TN concentrations than T0. Among them, T4 maintained a high final TN concentration of 22.90 g/kg, which was 11.17% higher than that of T0 and close to the highest value observed among all treatments. However, inorganic nitrogen responses differed among treatments: T1 showed more efficient conversion of NH4+-N to NO3−-N, whereas T3 had the lowest NH4+-N/NO3−-N ratio, indicating relatively higher compost maturity. Therefore, the nitrogen results should be interpreted on a concentration basis rather than as evidence of reactor-scale total nitrogen retention or nitrogen loss. Wood vinegar also affected phosphorus transformation, with T4 achieving a higher available phosphorus concentration. Bacterial community analysis showed that Bacillota and Actinomycetota dominated the composting process, and T4 maintained a more stable bacterial community structure during maturation. T4 was enriched in potential functional genera, including Saccharomonospora and Thermoactinomyces. Spearman correlation analysis indicated that Weissella and Pediococcus were significantly associated with nitrogen-related parameters in T4, whereas Saccharomonospora was closely linked to phosphorus transformation. PICRUSt2-based COG prediction further suggested that T4 maintained relatively stable nitrogen- and phosphorus-related functional potentials.
Full article
(This article belongs to the Special Issue Anaerobic Fermentation of Agricultural Waste and Sustainable Bioenergy Recovery)
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Open AccessArticle
Impact of Financial Reform on High-Quality Entrepreneurship Among Farmers: Evidence from a Quasi-Natural Experiment Based on the Multi-Period Difference-in-Differences Method
by
Dianwei Zhang, Yun Yu, Ruixiang Jing, Tao Li and Qian Lu
Agriculture 2026, 16(18), 1994; https://doi.org/10.3390/agriculture16181994 - 17 Sep 2026
Abstract
Financial reform has become a key driver in promoting high-quality entrepreneurship among farmers and fostering rural economic development. This study exploits the establishment of national-level Financial Reform Pilot Zones (FRPs) as a quasi-natural experiment. By applying a time-varying difference-in-differences (DID) model to seven
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Financial reform has become a key driver in promoting high-quality entrepreneurship among farmers and fostering rural economic development. This study exploits the establishment of national-level Financial Reform Pilot Zones (FRPs) as a quasi-natural experiment. By applying a time-varying difference-in-differences (DID) model to seven waves of China Family Panel Studies (CFPS) data (2010–2022), we find that: (1) Financial reform significantly promotes high-quality entrepreneurship among farmers. (2) This effect is primarily driven by the expansion of digital inclusive finance, while the social credit system serves as a significant positive moderator in the relationship between financial reform and entrepreneurial quality. (3) Further analysis reveals significant heterogeneity across different functional types of FRPs and across different industry. The overarching goal of this study is to causally identify whether and how financial reform influences the quality of rural entrepreneurship. Our findings provide empirical evidence and scalable insights for developing economies seeking to implement financial supply-side structural reform.
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(This article belongs to the Section Agricultural Economics, Policies and Rural Management)
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Open AccessEditorial
Advances and Challenges of Intelligent Technologies in Farm Animal Disease, Feeding and Building Environmental Control
by
Liqiang Han
Agriculture 2026, 16(18), 1993; https://doi.org/10.3390/agriculture16181993 - 17 Sep 2026
Abstract
The global demand for high-quality animal products is driving the rapid development of livestock farming [...]
Full article
(This article belongs to the Special Issue Application of Intelligent Technologies in Farm Animal Disease, Feeding and Building Environmental Control)
Open AccessReview
Nutritional Value and Safety of Lupin-Based Food and Feed Systems: Diaporthe toxica, Phomopsins, Processing Effects, and Analytical Control
by
Daria Padewska, Marcin Bryła and Marek Roszko
Agriculture 2026, 16(18), 1992; https://doi.org/10.3390/agriculture16181992 - 17 Sep 2026
Abstract
Lupin is an increasingly important protein crop, but evidence linking its nutritional and technological potential with fungal contamination, processing safety, and analytical control remains fragmented. This review integrates these areas within a field-to-product assessment of lupin-based food and feed systems, explicitly distinguishing among
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Lupin is an increasingly important protein crop, but evidence linking its nutritional and technological potential with fungal contamination, processing safety, and analytical control remains fragmented. This review integrates these areas within a field-to-product assessment of lupin-based food and feed systems, explicitly distinguishing among evidence obtained directly from lupin, findings generated in other legume matrices, and mechanistic inferences. Diaporthe toxica can colonize lupin tissues and seeds, providing a pathway for phomopsins to enter food and feed chains; however, occurrence data are scarce, geographically limited, and focused mainly on phomopsin A (PHO-A). Toxicological evidence is derived predominantly from animal studies, and no health-based guidance values have been established for phomopsins. Processing can reduce quinolizidine alkaloids and other antinutritional constituents, but direct evidence that soaking, heating, fermentation, or germination eliminates phomopsins from lupin is lacking. PHO-A persistence has been demonstrated in artificially contaminated pea-based products. These findings indicate that conventional heating cannot be assumed to detoxify contaminated lupin, but they do not provide quantitative retention data for lupin matrices. LC-MS/MS is currently the most suitable analytical approach, although heterogeneous contamination, insufficiently described sampling, matrix effects, variable sample preparation, and the limited availability of analytical standards and reference materials constrain data comparability. Research priorities include representative occurrence monitoring, validated methods covering relevant lupin matrices, mass-balance processing studies using contaminated lupin, characterization of transformation products, and exposure assessment. This integrated framework distinguishes established evidence from model-derived findings and unresolved knowledge gaps, thereby supporting risk-based control from crop production to final products.
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(This article belongs to the Section Agricultural Product Quality and Safety)
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Open AccessArticle
Age-Dependent Impact of Dietary Supplements on Gross Energy Content in Worker Honey Bees (Apis mellifera carnica, Pollmann 1879)
by
Ana-Marija Kovač, Ivana Tlak Gajger and Maja Ivana Smodiš Škerl
Agriculture 2026, 16(18), 1991; https://doi.org/10.3390/agriculture16181991 - 16 Sep 2026
Abstract
Nutritional supplementation with pollen and protein-based patties is widely used in apiculture to support honey bee (Apis mellifera carnica) colonies during periods of limited natural forage, particularly in early spring and late summer. This study evaluated the effects of different supplemental
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Nutritional supplementation with pollen and protein-based patties is widely used in apiculture to support honey bee (Apis mellifera carnica) colonies during periods of limited natural forage, particularly in early spring and late summer. This study evaluated the effects of different supplemental diets on the survival, food intake, and gross energy content of caged adult worker bees of mixed ages under controlled laboratory conditions. Worker bees were fed four different diets: sucrose syrup (control), sugar patty, protein patty, and pollen patty. Survival, food intake, and gross energy content were assessed over an 18-day experimental period. Survival differed significantly among dietary treatments in both spring and summer experiments, with the highest final survival observed in bees receiving sugar patty (HBP). Aged-related differences in survival were treatment-dependent. Descriptive calorimetric data showed that bees receiving the pollen-enriched patty (HBP-PO) had the highest mean GE of bee body (22.05 ± 0.38 MJ/kg), with similarly high values observed in the 18-day old bees (22.28 ± 0.18 MJ/kg) and 20-day old bee workers (22.26 ± 0.23 MJ/kg). Notably, the GE content of the experimental diets did not correspond directly to the GE measured in bee bodies: the sucrose syrup had the highest dietary GE (16.56 ± 0.01 MJ/kg), whereas bees fed pollen-enriched patty showed the highest descriptive mean GE despite this diet having the lowest GE among the tested patties. These findings indicate that dietary caloric density alone may not predict the energetic status or survival of worker bees and suggest that diet composition and worker age contribute to different physiological responses to supplemental feeding. The experiment was conducted under controlled cage conditions; further studies at the colony level under apiary conditions are needed to determine whether these individual-level responses are maintained within functioning honey bee colonies.
Full article
(This article belongs to the Special Issue Agricultural Stressors Shaping Disease and Immune Responses in Honey Bees)
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Open AccessReview
Material Selection for Soil Remineralization: Mineralogical, Agronomic and Environmental Perspectives on Crushed Rock Wastes
by
Janaína Oliveira Gonçalves, Maria Salas Villa, Magdalena Bermudez, Bruna Silva de Farias, Eduardo Silveira Ribeiro, Karen Esther Muñoz Salas, Renata Machado Pereira Da Silva and Sibele Santos Fernandes
Agriculture 2026, 16(18), 1990; https://doi.org/10.3390/agriculture16181990 - 16 Sep 2026
Abstract
The increasing interest in the use of crushed rock wastes as soil remineralizers has created a need for a more rigorous evaluation of these materials. This evaluation should go beyond total nutrient content and consider the factors that may determine their performance under
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The increasing interest in the use of crushed rock wastes as soil remineralizers has created a need for a more rigorous evaluation of these materials. This evaluation should go beyond total nutrient content and consider the factors that may determine their performance under agricultural conditions. Therefore, this review aims to analyze these wastes from an integrated perspective, considering their potential for nutrient supply, their possible agronomic responses, and their environmental and operational feasibility. The literature was selected using different research platforms, prioritizing recent scientific studies directly related to the scope of the review. The selected studies indicate that high nutrient contents in rocks do not necessarily guarantee their availability, while mineralogy, pH, water availability, temperature, and microbial activity are among the factors that determine the rate and duration of nutrient release. These differences are also reflected in crop responses, which vary according to the type of rock material, soil conditions, application rate, and crop species. While some studies report improvements in soil fertility and crop yield, others show changes in soil chemical properties and evidence of mineral weathering without corresponding increases in productivity. At the same time, strategies used to increase material reactivity, such as reducing particle size, may lead to greater energy demand and potentially higher CO2 emissions associated with grinding. Another important aspect is that materials with favorable nutrient composition may have their agricultural use limited by the presence and mobility of potentially toxic elements. Based on these findings, this review organizes the selection of crushed rock wastes through a critical assessment of their reactivity and compatibility, while also considering environmental safety and conditions that may determine their agricultural feasibility.
Full article
(This article belongs to the Special Issue Crushed Rock Wastes for Soil Remineralization: Potential Applications for Sustainable Agriculture)
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Open AccessArticle
Long-Term Combined Organic and Mineral Fertilization Modulates Aggregate-Associated Organic Carbon Pool and Improves Carbon Sequestration in Aeolian Sandy Soils
by
Yuxin Wang, Yue Wang, Junmei Shi, Xingtong Lv, Xinhao Gong, Jinfeng Yang and Xiaori Han
Agriculture 2026, 16(18), 1989; https://doi.org/10.3390/agriculture16181989 - 16 Sep 2026
Abstract
Aeolian sandy soils are typically characterized by low organic matter content and loose structural stability, which severely restrict soil carbon sequestration capacity and farmland productivity. Soil aggregates are the primary carriers and protective barrier for soil organic carbon (SOC), dominating the processes of
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Aeolian sandy soils are typically characterized by low organic matter content and loose structural stability, which severely restrict soil carbon sequestration capacity and farmland productivity. Soil aggregates are the primary carriers and protective barrier for soil organic carbon (SOC), dominating the processes of SOC accumulation and stabilization. Long-term fertilization is a critical field management strategy for regulating soil structure and carbon sequestration; however, the way in which both applying organic fertilizer alone or in combination with mineral fertilizer mediate aggregate distribution, structural stability, and aggregate-associated SOC sequestration in aeolian sandy soils under continuous peanut monoculture conditions remains largely unexplored. This study was based on a 16-year continuous field fertilization experiment conducted on aeolian sandy soil in Northeast China. The experimental treatments included: CK (control, no fertilization), NPK (balanced mineral fertilization), M (organic fertilizer alone), and MNPK (a combination of organic and mineral fertilizers). The topsoil samples (0–20 cm) were collected after the peanut harvest. The aggregate was classified into four fractions using the wet-sieving method: coarse macro-aggregate (>2 mm), fine macro-aggregate (0.25–2 mm), micro-aggregate (0.053–0.25 mm), and the silt–clay fraction (<0.053 mm). The aggregate size distribution, aggregate stability indices (MWD and GMD), SOC concentration, and SOC stock were measured under different fertilization treatments. The results indicated that long-term fertilization markedly promoted the formation of macroaggregates (>0.25 mm) and enhanced the soil aggregate stability. The addition of manure resulted in significantly better effects than the application of mineral fertilizers alone. In comparison with the CK, SOC stocks in the NPK, M and MNPK treatments increased by 18.3%, 44.9%, and 65.5%, respectively. The SOC stock (9.3 Mg·ha−1) and peanut yield were highest under the MNPK treatment. With equal exogenous manure-carbon inputs for the M and MNPK treatments, a strong organic–mineral synergistic effect was observed in the MNPK treatment. This synergy reinforced the sequestration of exogenous organic carbon. Correlation analysis confirmed that SOC sequestration in aeolian sandy soil was predominantly governed by macroaggregate formation and internal carbon enrichment, rather than changes in microaggregate and silt–clay fractions. Our results reveal that combined organic–mineral fertilization effectively facilitates macroaggregate formation, improves aggregate stability and enhances organic carbon sequestration in macroaggregates of aeolian sandy soils, while increasing crop yield. Hence, organic–mineral combined fertilization is a promising agronomic practice for soil improvement, carbon sequestration and sustainable production in aeolian-sandy peanut cropping systems, offering practical field references for low-carbon sustainable management of sandy farmland.
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(This article belongs to the Section Agricultural Soils)
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Open AccessArticle
Effects of Supplementary Fertilization on Physiological Traits and Yield of Soybean Following Drought-Flood Abrupt Alternations of Varying Intensities
by
Yingjun She, Yanglantian Song, Zeqin Lai, Jiajia Liu, Yingkun Yu, Ping Li, Zihan Zhao, Jixiao Wu, Wenbo Yue, Zizhe Zhou, Jun Wei, Zhiliang Zhang, Caixia Zheng and Xiaopei Tang
Agriculture 2026, 16(18), 1988; https://doi.org/10.3390/agriculture16181988 - 16 Sep 2026
Abstract
Drought-flood abrupt alternation (DFAA) events severely constrain soybean production, yet the physiological effects of supplementary fertilization and other measures following such events are unclear. This study investigated the effects of supplementary fertilization (SF) on the physiological characteristics and yield of soybean after different
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Drought-flood abrupt alternation (DFAA) events severely constrain soybean production, yet the physiological effects of supplementary fertilization and other measures following such events are unclear. This study investigated the effects of supplementary fertilization (SF) on the physiological characteristics and yield of soybean after different intensities of DFAA events. Seven treatments were set up in the experiment: drought rapidly followed by light, moderate, or heavy waterlogging (D-LW, D-MW, D-HW), SF after DFAA (D-LWF, D-MWF, D-HWF), and normal water supply (control, CK). Soybean growth, leaf physiological indicators, above-ground dry matter, yield, and yield components were measured throughout the experiment. The results showed that SF promoted stem diameter and leaf growth in soybeans under drought-to-waterlogging stress. At 35 days after fertilization, the D-HWF treatment significantly outperformed D-HW in stem diameter and leaf area. At 10 days after fertilization, compared with the corresponding unfertilized treatments, the D-L/-M/-HWF treatments significantly increased leaf relative water content (LRWC, by 13.55–33.39%) and SOD activity, while the D-M/-HWF treatments reduced the chlorophyll a/b ratio by 6.14–8.29%. Under uniform SF application, yield-related traits exhibited a statistically non-significant positive recovery trend under the D-MW and D-HW treatments (except for the effective grain number and total grain number). The D-MWF and D-HWF combinations significantly increased effective grain number and total grain number by 39.20–88.59% relative to their unfertilized controls. SF application tended to enhance soybean yield under DFAA, with D-MWF and D-HWF showing significant yield increases of 63.24% and 57.04% over D-MW and D-HW, respectively. The yield compensation rate rose substantially, with fertilized treatments recovering to 71.28–74.69% of the CK level. Collectively, these results indicate that SF alleviates yield loss under DFAA stress through a combination of optimized pod traits, increased grain number, alleviated oxidative damage during later recovery stages, and promoted regrowth. Under the present experimental conditions, greater compensatory effects were observed under moderate and heavy post-drought waterlogging than under light post-drought waterlogging, reflecting the combined influences of DFAA severity and the duration of pre-fertilization recovery intervals.
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(This article belongs to the Section Crop Production)
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Open AccessArticle
Evaluation of Rhizosphere Environment, Growth, and Yield Components of Rice Affected by No-Puddling and Mid-Season Drainage
by
Hyojeong Lee, Seung Ka Oh and Young-Son Cho
Agriculture 2026, 16(18), 1987; https://doi.org/10.3390/agriculture16181987 - 16 Sep 2026
Abstract
Traditional paddy cultivation relying on intensive puddling and continuous flooding faces severe sustainability challenges from labor shortages, soil degradation, and high greenhouse gas emissions. However, the interactive effects of combining no-puddling (NP) and mid-season drainage (MD) on the rhizosphere environment and yield remain
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Traditional paddy cultivation relying on intensive puddling and continuous flooding faces severe sustainability challenges from labor shortages, soil degradation, and high greenhouse gas emissions. However, the interactive effects of combining no-puddling (NP) and mid-season drainage (MD) on the rhizosphere environment and yield remain unclear. This two-year field study (2024–2025) evaluated the combined impact of tillage (puddling, P vs. NP) and water regimes (continuous flooding, CF vs. MD) on rice performance and soil properties. Combined MD-NP accelerated soil redox potential (Eh) recovery (up to −271 mV) during drainage, mitigating soil reduction toxicity. MD-NP effectively restricted excessive internode elongation, suppressed unproductive tillering, and maximized root dry weight at heading (8.38 g plant−1 in 2025; up to 29.5% higher than CF). In 2025, MD-NP achieved the highest brown rice yield (4.89 t ha−1; 20.4% higher than CF-P), primarily driven by increased grains per panicle (82.5) and ripened grain percentage (76.5%). Three-way ANOVA confirmed significant main effects of tillage (p = 0.036) and water management (p = 0.003) on yield. Additionally, MD-NP better preserved soil organic matter and total carbon. Overall, MD-NP provides a climate-resilient, low-carbon, and sustainable paddy management strategy that conserves water and labor while securing yield.
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(This article belongs to the Section Agricultural Systems and Management)
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Open AccessArticle
An Improved DeepLabv3+-Based Framework for Field-Road Extraction and Structural Indicator Quantification in Well-Facilitated Farmland
by
Yongsheng Liu, Chunling Chen, Sheng Xu, Shuai Feng, Zhonghui Guo and Tongyu Xu
Agriculture 2026, 16(18), 1986; https://doi.org/10.3390/agriculture16181986 - 16 Sep 2026
Abstract
Well-facilitated farmland plays an important role in stabilizing and increasing crop yields, advancing agricultural mechanization, and improving production efficiency. Field-road quality directly affects machinery access and the transport of agricultural inputs and harvested crops. Current acceptance inspections rely mainly on field surveys and
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Well-facilitated farmland plays an important role in stabilizing and increasing crop yields, advancing agricultural mechanization, and improving production efficiency. Field-road quality directly affects machinery access and the transport of agricultural inputs and harvested crops. Current acceptance inspections rely mainly on field surveys and spot measurements, resulting in limited spatial coverage, low efficiency, and poor reproducibility. Therefore, a method is needed to rapidly survey entire field-road networks, extract road extents and structural indicators, and generate verifiable inspection records. Centimeter-resolution UAV imagery enables flexible and repeatable data acquisition, but deriving acceptance-oriented indicators remains challenging. Narrow field roads are readily obscured by crops and shelterbelt shadows or confused with cropland textures, resulting in blurred boundaries, discontinuities, and false detections. We developed a lightweight UAV-based framework integrating field-road segmentation and structural-indicator quantification. MobileNetV2 replaced the DeepLabv3+ backbone, while a Normalization-based Attention Module and Content-Aware ReAssembly of FEatures enhanced interference suppression and spatial reconstruction. Morphological processing, skeleton extraction, Euclidean distance transformation, and skeleton-graph analysis were then used to quantify road width and network connectivity. Across three training runs with different random seeds, the model achieved mean mIoU, mPA, and precision values of 93.34%, 96.75%, and 98.90%, respectively. The model had 6.14 million parameters and an inference speed of 17.04 FPS. After averaging five measurements from each road segment, the R2 values between the predicted and manually measured widths were 0.650, 0.486, and 0.662 for asphalt, concrete, and gravel roads, respectively. The corresponding width MAEs were 0.130, 0.140, and 0.100 m. Connectivity analysis yielded an index of 1.00 in the first validation area, while gap repair increased the index from 0.4682 to 0.4795 in the second area. The framework supports efficient, quantitative, and traceable acceptance inspection of field-road infrastructure in well-facilitated farmland.
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(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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Open AccessArticle
Structure-Aware RGB Channel Reconstruction for YOLOv8-Based Oyster Mushroom Detection
by
Sonay Duman, Furkan Gözükara, Zeki Yetgin and Erdinç Avaroğlu
Agriculture 2026, 16(18), 1985; https://doi.org/10.3390/agriculture16181985 - 16 Sep 2026
Abstract
Oyster mushroom cultivation requires accurate object detection for automated monitoring and precision agriculture applications. This study evaluates a structure-aware three-channel input representation for YOLOv8s in which raw RGB channels are replaced by grayscale intensity, Sobel gradient magnitude, and a complementary structural channel derived
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Oyster mushroom cultivation requires accurate object detection for automated monitoring and precision agriculture applications. This study evaluates a structure-aware three-channel input representation for YOLOv8s in which raw RGB channels are replaced by grayscale intensity, Sobel gradient magnitude, and a complementary structural channel derived from Gaussian Blur, Laplacian of Gaussian (LoG), Canny edge detection, or Gabor filtering. The dataset contains 555 RGB images and 8282 maturity-labeled mushroom instances. Controlled experiments include an RGB baseline with HSV augmentation disabled, component-wise ablations (GGG and GGradG), repeated-seed training, a chronological holdout, and a cross-architecture RT-DETR evaluation. Under the fixed random split, differences among RGB, grayscale, and structure-aware inputs were modest, and the ablations indicate that grayscale conversion accounts for most of the measured effect. Performance decreased substantially under the chronological split, and RT-DETR did not reproduce the same ordering observed with YOLOv8s. These results show that input representation can influence detector behavior, but they do not support a general claim that handcrafted structural channels consistently improve robustness across evaluation protocols or architectures.
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(This article belongs to the Section Artificial Intelligence and Digital Agriculture)
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Open AccessArticle
A Comparative Study of Growth Performance, Small Intestinal Function, and Metabolite Composition of Duroc × Landrace × Yorkshire and Ningxiang Piglets During the Post-Weaning Period
by
Luya Feng, Jiayi Liu, Yunfang Song, Jing Wang, Xiaokang Ma and Bi’e Tan
Agriculture 2026, 16(18), 1984; https://doi.org/10.3390/agriculture16181984 - 16 Sep 2026
Abstract
The Ningxiang (NX) pig, recognized for its strong resistance as one of China’s esteemed indigenous breeds, has been the subject of limited investigation concerning its intestinal mucosal immune function. This study aimed to conduct a comparative analysis of the intestinal mucosal immune function
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The Ningxiang (NX) pig, recognized for its strong resistance as one of China’s esteemed indigenous breeds, has been the subject of limited investigation concerning its intestinal mucosal immune function. This study aimed to conduct a comparative analysis of the intestinal mucosal immune function and metabolites between Duroc × Landrace × Yorkshire (DLY) piglets and NX piglets. The findings revealed that NX piglets exhibited a significantly higher average daily feed intake, liver index and kidney index compared to their DLY counterparts. Notably, the serum concentration of interleukin-17 was markedly elevated in NX piglets. In terms of the mechanical barrier function, NX piglets demonstrated increased relative mRNA expression of Claudin2 and Occludin in the jejunal mucosa, along with higher Claudin3 and Occludin expression in the ileal mucosa. Furthermore, the relative mRNA expression of porcine β-defensin-1 was significantly higher in the jejunal mucosa of NX piglets. Conversely, expression levels of porcine β-defensin-2 and regenerating islet-derived 3 gamma were reduced in NX piglets compared to DLY piglets. Spearman’s correlation analysis in DLY and NX piglets revealed that the gene expression of proline-arginine-39 (PR-39) was significantly positively correlated with histamine and cholic acid, and significantly negatively correlated with the gene expression of interleukin-6. In summary, the results suggest that NX piglets possess superior intestinal mucosal immune functionality, offering new perspectives for comparative studies on the immune traits of different pig breeds. Moreover, this study identifies the antimicrobial peptide PR-39 as a key biomarker strongly correlated with specific metabolites and inflammatory responses, highlighting its potential as a co-dependent marker of genetic differences between DLY and NX piglets.
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(This article belongs to the Section Farm Animal Production)
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Open AccessArticle
Evaluating Marker-Assisted Selection for Varroa Resistance in Flanders Using a Three-Variant Model
by
Emma Bossuyt, Regis Lefebre, Ellen Danneels and Dirk C. de Graaf
Agriculture 2026, 16(18), 1983; https://doi.org/10.3390/agriculture16181983 - 16 Sep 2026
Abstract
Selective breeding for Varroa resistance is an important strategy to improve honey bee colony survival against Varroa destructor, which remains a major threat to their health. However, phenotyping resistance traits is often labor-intensive. Marker-assisted selection (MAS) offers an alternative by selecting colonies
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Selective breeding for Varroa resistance is an important strategy to improve honey bee colony survival against Varroa destructor, which remains a major threat to their health. However, phenotyping resistance traits is often labor-intensive. Marker-assisted selection (MAS) offers an alternative by selecting colonies based on molecular markers associated with resistance traits. This study evaluated the implementation of MAS for drone brood resistance (DBR, i.e., mite non-reproduction in drone brood), using a three-SNP model within the ongoing Flemish honey bee breeding program. From 2022 to 2025, predominantly Apis mellifera carnica workers and drones were genotyped. Through targeted breeding and mating via instrumental insemination, we aimed to reconstruct the genetic profile with all three SNPs’ protective alleles. Over sampling years, SNP2 and SNP6 increased in frequency in both workers and drones, whereas SNP4 did not. The cumulative three-SNP score also increased over years and generations, with the greatest genetic gain achieved by combining targeted breeding and targeted mating. The ideal genetic profile was obtained in three queens. However, genetic progress was not reflected in the DBR phenotype. These findings provide insights into the potential and limitations of MAS, suggesting future efforts in breeding programs towards genomic selection.
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(This article belongs to the Section Farm Animal Production)
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Open AccessArticle
Design and Optimization of a Vibratory Device for Embryo-Oriented Single-Row Conveying of Rice Seeds
by
Junjie Yan, Mingxuan Lu, Huiping Huang, Dongxin Guo, Xiangyun Sun and Tianyu Liu
Agriculture 2026, 16(18), 1982; https://doi.org/10.3390/agriculture16181982 - 16 Sep 2026
Abstract
To address single-row feeding and embryo-orientation requirements in rice-seed preprocessing, a two-stage cooperative vibratory conveying and orientation device was developed for one tested batch of the long-grain hybrid indica cultivar ‘Y Liangyou 900’. The circular module fluidizes bulk seeds and forms a single-row
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To address single-row feeding and embryo-orientation requirements in rice-seed preprocessing, a two-stage cooperative vibratory conveying and orientation device was developed for one tested batch of the long-grain hybrid indica cultivar ‘Y Liangyou 900’. The circular module fluidizes bulk seeds and forms a single-row stream, and the linear module uses stepped V-shaped grooves for passive posture correction. For 50 individually identified seeds, repeated camera measurements gave a longitudinal embryo-end center-of-mass offset of 0.285 ± 0.040 mm (mean ± sample SD; range 0.215–0.344 mm; CV 14.1%). Five tipping trials per seed at 200 V, 100 Hz, a 6 mm step, and a 9° chute showed a positive but overlapping relationship between Δd and tipping probability; threshold screening placed the effective stabilizing moment Msave,eff on the order of 10−7 N·m. At 100 Hz, PCB M352C68 accelerometer measurements with five 10 s repeats per voltage gave amplitude equivalents of 0.100, 0.137, and 0.176 mm at 160, 180, and 200 V, corresponding to peak accelerations of 4.03, 5.52, and 7.09 g. Five paired physical-simulation calibration tests gave mean absolute relative errors of 0.89% for angle of repose and 0.43% for bulk density. Across 11 conveying conditions, DEM and bench throughputs ranged from 180–208 and 176–204 grains/min, respectively, with a mean absolute percentage error of 1.98%. Orthogonal testing selected a 6 mm step height, 9° chute inclination, 200 V linear-vibrator setpoint, and 100 Hz vibration frequency; ten independent 200-seed verification runs (2000 seeds total) gave mean forward-retention and reverse-correction rates of 92.4% and 88.1%. The archived verification records preserve aggregate means but not the complete run-level values required for dispersion estimates. Integrated optimization selected a conveyor-belt speed of 31.5 mm/s, a 200 V setpoint, and a 5.30 mm transfer drop height; validation means were 91.2% orientation success and 14.5% seed-flow-uniformity CV. In an unreplicated condition-level robustness screen, lower performance values were descriptively observed at high moisture and under dusty or broken-glume conditions. Because each condition was evaluated only once, these observations do not constitute statistical evidence of robustness or factor effects. The findings therefore remain specific to the tested cultivar, conditions, and short-run protocol.
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(This article belongs to the Section Seed Science and Technology)
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Nano-Enabled Zinc Fertilization Alters Targeted Organosulfur Metabolite Profiles in Garlic (Allium sativum L.)
by
Addisie Geremew, Alemayehu Shembo, Elisha Peace, Xingmao Ma and Laura Carson
Agriculture 2026, 16(18), 1981; https://doi.org/10.3390/agriculture16181981 - 16 Sep 2026
Abstract
Garlic (Allium sativum L.) is a major horticultural crop valued for its distinctive flavor, nutritional quality, and health-promoting properties, including antioxidant and antimicrobial activities. The characteristic aroma and health benefits of garlic are primarily attributed to its organosulfur compounds. This study evaluated
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Garlic (Allium sativum L.) is a major horticultural crop valued for its distinctive flavor, nutritional quality, and health-promoting properties, including antioxidant and antimicrobial activities. The characteristic aroma and health benefits of garlic are primarily attributed to its organosulfur compounds. This study evaluated the effects of conventional zinc sulfate (ZnSO4) and zinc oxide nanoparticles (ZnO NPs) on selected organosulfur metabolites in garlic bulbs using targeted liquid chromatography–tandem mass spectrometry (LC–MS/MS) and gas chromatography–mass spectrometry (GC–MS). Treatments comprised an untreated control, ZnSO4, 25 mg ZnO NPs kg−1 soil (ZnO NP-25), and 55 mg ZnO NPs kg−1 soil (ZnO NP-50), with ten independent experimental units per treatment. Relative to the control, ZnO NP-50 increased alliin by 225.3%, allicin by 309.6%, diallyl sulfide (DAS) by 219.5%, diallyl disulfide (DADS) by 231.7%, and diallyl trisulfide (DATS) by 149.7%, whereas ajoene decreased by 67.3%. ZnO NP-50 produced the highest mean concentrations of alliin, allicin, DAS, DADS, and DATS, indicating a dose-related compositional pattern across the two nanoparticle rates. These findings demonstrate that soil-applied ZnO NPs altered the targeted organosulfur profile of garlic under greenhouse conditions. The physiological and molecular mechanisms underlying these responses were not measured and require further investigation.
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(This article belongs to the Special Issue Research on Secondary Metabolites in Agricultural Plants)
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