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Agriculture

Agriculture is an international, peer-reviewed, open access journal published semimonthly online. 

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Fertilization is a regular management approach that can enhance soil fertility and stimulate the proliferation of beneficial microorganisms. However, the prolonged influence of fertilization practices on soil quality, microbial functional characteristics, and the underlying mechanisms still remain incompletely understood. In this study, we examined the impact of various fertilization strategies on the soil quality index (SQI) and community-level physiological profiles (CLPP) during two crop seasons (maize and soybean, respectively) in a 45-year field trial. Four treatments were implemented: unfertilized control (CK), inorganic nitrogen–phosphorus–potassium fertilizer (NPK), organic fertilizer (M), and organic–inorganic fertilization (MNPK). Results showed that across both seasons, NPK application reduced soil pH and the McIntosh index, whereas organic amendments (M and MNPK) notably enhanced total and available nutrients, SQI, microbial biomass, and enzyme activities. CLPP analysis revealed that organic fertilization significantly enhanced microbial metabolic activity and functional diversity, particularly boosting the utilization of carbohydrates (20–38%) and carboxylic acids (18–36%). Random forest modelling indicated available potassium (AK) as the primary driver of carbon metabolic activity in both seasons, revealing its critical role in regulating microbial functions. Functional metabolic diversity during the maize season was most strongly influenced by microbial entropy (qMB), whereas in the soybean season, it was available nitrogen (AN). Additionally, organic fertilization led to an indirect improvement in SQI during the maize and soybean seasons by increasing microbial biomass. In conclusion, the study underscored the importance of long-term organic fertilization for improving soil quality and provided empirical evidence to maintain the sustainable practices of agriculture in Northeast China.

19 March 2026

Study area of the long-term field experiment on brown soil fertilizer. (A) Liaoning Province (B) Shenyang city (C) Brown soil study area. The red box represents the plot in the experimental site.

Rice Straw as Growth Substrate for Soilless Sod Production

  • Baohua Chu,
  • Sangyuan Hu and
  • Zhaolong Wang
  • + 2 authors

Rice straw as a growth substrate for soilless sod production not only avoids the damage to farmland soil deterioration but also solves the difficulty in disposing of a large amount of agricultural straw waste. This study was designed to explore the feasibility of using rice straw as a soilless sod production for seashore paspalum. The results showed that both fermented rice straw and raw rice straw significantly promoted the creeping growth and tillering of seashore paspalum, shortening the sod production period, when compared to the conventional soil sod. Rice straw sod significantly reduced sod weight to 50% and 52% of the soil sod, but increased sod strength to avoid tear damage in handling and transportation. Rice straw sod had 2 d longer shelf life than the soil sod, with slower decline of sod quality and maintained higher root and leaf emergence vigor during the sod storage. After sod installation, rice straw sod showed higher numbers of root and leaf emergence, and higher green leaves, stolons, new roots, aboveground and underground biomass, but lower thatch biomass, compared to the soil sod. Our results demonstrated that using rice straw as a growth substrate to produce soilless sod is feasible and significantly better than conventional soil sod production.

19 March 2026

Turfgrass growth during sod production in rice straw substrate and soil. * indicates significant difference between straw and soil sod productions (p < 0.05). (A): Turfgrass coverage in 2024. (B): Photos of turfgrass coverage in 2024. (C): Turfgrass coverage in 2025. (D): Photos of turfgrass coverage in 2025. (E): Creeping growth rate in 2024. (F): Creeping growth rate in 2025. (G): Tillering rate in 2025.

Modern agriculture faces increasing pressure to maintain productivity while reducing soil degradation, chemical inputs, and ecological footprint, making biologically based soil-improvement strategies highly relevant. This study examined whether microbial inoculation, combined with conservation tillage practices (loosening and no-tillage), can enhance soil physical quality during pea (Pisum sativum) cultivation in an agroecological market garden in Hungary. A 2 × 2 factorial field experiment was established, testing tillage (loosening vs. no-tillage) and microbial inoculation (with vs. without) in a randomized design with three replications per treatment (12 plots total). A single microbial application was performed prior to planting using a consortium of Rhizobium spp., Ensifer spp., Pseudomonas spp., and Bacillus spp. The research focused on (I) soil penetration resistance, (II) soil moisture dynamics, and (III) infiltration capacity, with most parameters measured before and after planting. Microbial inoculation significantly reduced penetration resistance under both tillage systems and influenced soil moisture behavior, indicating improved soil structure and water retention. Infiltration rate did not change significantly within the study period. Overall, the results demonstrate that microbial amendments can rapidly improve key soil physical properties, offering a practical, nature-based strategy for resilient, low-input farming systems.

19 March 2026

Experimental layout of the field plots used during the study (Source: Own work).

This research aimed to investigate livestock owners’ willingness to pay (WTP) for a hypothetical livestock vaccination program in southern Ethiopia. We used both primary and secondary data. The former data were collected from 377 livestock farmers. We used descriptive statistics and a seemingly unrelated bivariate probit model to analyze the data. The double-bounded contingent valuation format was used within a seemingly unrelated bivariate probit model to identify factors influencing WTP for livestock vaccination. It was found that the average WTP for sheep and goat vaccination was 0.38 USD (United States of America dollar) yearly, while for cattle and oxen vaccination, it was 0.64. Factors such as the household head’s education, sex, and age; annual income; total livestock holdings; and farm size affected farmers’ WTP for sheep and goat vaccination in both initial and follow-up scenarios. Market access, farm experience, and access to credit also influenced WTP for cattle and oxen vaccination across both bid values. We suggest that improving socioeconomic factors, such as household well-being and access to credit services, is vital because these elements directly influence a household’s capacity to invest in livestock health and productivity. Establishing clear vaccination rates, with vaccine costs aligned to the average household income, is recommended.

19 March 2026

Theoretical framework of the study.

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Agriculture - ISSN 2077-0472