Methods and Protocols, Volume 8, Issue 6

Machine learning (ML) excels over regression by automatically capturing complex, non-linear relationships and interactions, enabling more flexible and accurate predictions without strict assumptions. This study focuses on developing ML-based predicti...

The increasing frequency, duration, and intensity of wildfires over the past decade have raised significant concerns about widespread exposure to wildfire smoke. Inhalation of wildfire smoke poses a substantial risk to human health, with epidemiologi...

A straightforward whole-mount approach has been developed that uses fluorescence imaging, mouse trachea, and a range of off-the-shelf reagents for rapidly evaluating substance toxicity within the ciliated respiratory epithelium. Using this protocol,...

L-Carnitine (L-CAR) and acetyl-L-carnitine (Acetyl L-CAR) are the essential cofactor compounds in lipid metabolism and are used in the treatment of various diseases. The European Food Safety Authority (EFSA) has reported that Acetyl-L-CAR contributes...

Investigation of molecular mechanisms that underlie the toxicity of reactive oxidants requires the usage of reductionist cellular models, where laboratory cultures are treated by known doses of the target compounds in strictly controlled conditions....

Drug discovery remains a time-consuming and costly process, necessitating innovative computational approaches to accelerate early stage target identification and compound development. We introduce AgentMol, a modular multimodel AI system that integra...

We previously developed a bead-coupled ligase detection reaction (LDR) assay that enables simple and rapid detection of single-nucleotide variations (SNVs) using synthetic oligonucleotide templates. In the present study, this approach was extended to...

Retinal detachment (RD) disrupts the eye’s immune-privileged status, causing a local inflammatory response that contributes to adverse clinical outcomes, including proliferative vitreoretinopathy and suboptimal visual recovery. Comprehensive pr...

We have developed a method for in vivo quantitation of lung delivery of inhaled nebulized drugs by measuring a fluorescent-labeled analog in bronchioalveolar lavage fluid (BALF) collected immediately after inhalation dosing. The effectiveness of deli...

The presence of antioxidants in food contributes to the preservation of its taste and technological qualities, preventing its spoilage for a longer time, which is important at all stages of production and storage. The major antioxidants are vitamins,...