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Feature Papers in Methods and Protocols 2025
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Feature Papers in Methods and Protocols 2025

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1762

Special Issue Editor

Prof. Dr. Fernando Albericio

E-Mail Website
Guest Editor
1. School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa
2. Department of Organic Chemistry, University of Barcelona, Barcelona, Spain
Interests: antimicrobial peptides; solid-phase chemistry; combinatorial chemistry; drug delivery systems; peptide drug conjugates; orthogonal chemistry; drug discovery; biomaterials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are glad to announce this Special Issue on “Feature Papers in Methods and Protocols 2025”. In this Special Issue, we will collect articles from top researchers describing new approaches or new cutting-edge developments to tackle questions in the fields of life sciences, chemistry, and biomedical sciences. In particular, we encourage submissions in the emerging fields of “Omics and High Throughput”, “Public Health Research”, “Synthetic and Systems Biology” and “Tissue Engineering and Organoids”. Our aim is to encourage scientists to publish new protocols based on established techniques, as well as significant improvements and developments of cutting-edge methods. For all articles, background information necessary to understand the underlying principles, full experimental details, and comparison with available protocols/methods must be provided. Scientists are encouraged to provide accompanying video demonstrations, which will be published online, for ease of reproducibility.

You are welcome to send short proposals for the submission of feature papers to our Managing Editor Ms. Eunice Fu(eunice.fu@mdpi.com) or Editorial Office (mps@mdpi.com) before submission. All papers will be subjected to thorough and rigorous peer review.

We look forward to receiving your excellent work.

Prof. Dr. Fernando Albericio
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cell biology
  • biochemistry
  • chemistry
  • nanotechnology
  • biotechnology
  • imaging
  • applied computation and bioinformatics
  • genomics
  • proteomics
  • developmental biology
  • model organisms
  • pharmacology
  • structural biology/chemistry
  • biophysics
  • tissue engineering
  • public health

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  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
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  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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22 pages, 3360 KiB  
Article
Experimental Investigation of the Effect of Solvent Type and Its Concentration on the Performance of ES-SAGD
by Sajjad Esmaeili, Brij Maini, Zain Ul Abidin and Apostolos Kantzas
Methods Protoc. 2025, 8(2), 39; https://doi.org/10.3390/mps8020039 - 8 Apr 2025
Viewed by 477
Abstract
Steam-assisted gravity drainage (SAGD) is a widely used thermal enhanced oil recovery (EOR) technique in North America, particularly in high-permeability oil sand reservoirs. While effective, its economic viability has declined due to low oil prices and high greenhouse gas (GHG) emissions from the [...] Read more.
Steam-assisted gravity drainage (SAGD) is a widely used thermal enhanced oil recovery (EOR) technique in North America, particularly in high-permeability oil sand reservoirs. While effective, its economic viability has declined due to low oil prices and high greenhouse gas (GHG) emissions from the steam generation. To improve cost-effectiveness and reduce emissions, solvent-assisted SAGD techniques have been explored. Expanding Solvent-SAGD (ES-SAGD) involves co-injecting light hydrocarbons like propane or butane with steam to enhance oil viscosity reduction. This approach lowers the steam–oil ratio by combining solvent dissolution effects with thermal effects. However, the high cost of solvents, particularly butane, challenges its commercial feasibility. Propane is cheaper but less effective, while butane improves performance but remains expensive. This research aims to optimize ES-SAGD by using a propane–butane mixture to achieve efficient performance at a lower cost than pure butane. A linear sand pack is used to evaluate different propane/butane compositions, maintaining constant operational conditions and a solvent concentration of 15 vol.%. Temperature monitoring provides insights into steam chamber growth. Results show that solvent injection significantly enhances ES-SAGD performance compared to conventional SAGD. Performance improves with increasing butane concentration, up to 80% butane in the C3–C4 mixture at the test pressure and ambient temperature. Propane alone results in the lowest system temperature, while conventional SAGD reaches the highest temperature. These findings highlight the potential of optimized solvent mixtures to improve ES-SAGD efficiency while reducing costs and GHG emissions. Full article
(This article belongs to the Special Issue Feature Papers in Methods and Protocols 2025)
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Review

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11 pages, 749 KiB  
Review
The PROMISE of Precision Medicine in Myocardial Infarction with Non-Obstructive Coronary Arteries
by Giulia La Vecchia, Vincenzo Scarica, Ludovica Leo and Rocco A. Montone
Methods Protoc. 2025, 8(3), 44; https://doi.org/10.3390/mps8030044 - 27 Apr 2025
Viewed by 511
Abstract
Myocardial infarction with non-obstructive coronary arteries (MINOCA) is a working diagnosis encompassing several pathophysiological mechanisms with specific treatments and different prognoses. Despite the absence of obstructive coronary artery disease, MINOCA has proven to be associated with a significant risk of mortality, angina burden, [...] Read more.
Myocardial infarction with non-obstructive coronary arteries (MINOCA) is a working diagnosis encompassing several pathophysiological mechanisms with specific treatments and different prognoses. Despite the absence of obstructive coronary artery disease, MINOCA has proven to be associated with a significant risk of mortality, angina burden, and socioeconomic costs. However, due to the heterogeneous nature of this clinical condition and the absence of randomized clinical trials, evidence supporting a standardized diagnostic algorithm and the clinical management of these patients is lacking. The PROMISE trial is the first randomized clinical trial evaluating the effectiveness of a precision medicine approach strategy in improving the outcomes and quality of life of patients with MINOCA, offering new insights into personalized treatment strategies. This review article discusses the promise of a precision medicine approach in patients with MINOCA, highlighting the potential innovations and challenges of a personalized medicine strategy in MINOCA. Full article
(This article belongs to the Special Issue Feature Papers in Methods and Protocols 2025)
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