Agronomy

19 pages, 3049 KB

Open AccessArticle

Ethanol Addition Significantly Improves Algal Biofertilizer Quality and Enhances Suitability of Wastewater for Irrigation in Treating Swine Wastewater with Dictyosphaerium sp.

by Xiaoyan Zhang, Mengjie Zhang, Hui Lin, Huabao Zheng and Qifa Zhou

Agronomy 2026, 16(9), 941; https://doi.org/10.3390/agronomy16090941 - 6 May 2026

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Abstract

Microalgae-based wastewater treatment can support sustainable crop production. This study evaluated whether ethanol supplementation improves swine wastewater (SW) treatment by Dictyosphaerium sp. and enhances algal biofertilizer production. Across the ethanol levels tested, 500 mg/L ethanol significantly promoted algal growth and enhanced liquid-phase net [...] Read more.

Microalgae-based wastewater treatment can support sustainable crop production. This study evaluated whether ethanol supplementation improves swine wastewater (SW) treatment by Dictyosphaerium sp. and enhances algal biofertilizer production. Across the ethanol levels tested, 500 mg/L ethanol significantly promoted algal growth and enhanced liquid-phase net removal of total salts, carbonate/bicarbonate, ammonium, phosphate, and calcium. Ethanol supplementation also reduced apparent nitrogen loss, and no residual ethanol was detected at the end of the culture. In the biofertilizer production experiment, peak algal biomass, algal nitrogen, and algal phosphorus increased by 320.0–407.4%, 122.7–158.1%, and 100.0–170.0%, respectively. Metatranscriptomic analysis showed active transcription of adh, aldh/aldB, and acs in Dictyosphaerium sp. and some bacterial taxa, mainly Flavobacterium, Chryseobacterium, Comamonas, and Brevundimonas. Community and transcriptomic results indicate enhanced photosynthetic activity and taxon-specific N- and P-related transcriptional responses, consistent with altered nitrate/nitrite transformation potential and increased nitrogen retention in the algal–bacterial system. Under the tested conditions, ethanol supplementation shows promise for SW treatment and algal biofertilizer production. Full article

(This article belongs to the Special Issue Agricultural Residue Utilization for Development of Biofertilizers)

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20 pages, 2442 KB

Open AccessArticle

Optimization of Key Techniques for In Vitro Rapid Propagation of New Edible and Ornamental Lily Cultivars

by Yuanjun Jiang, Zhengquan He, Manman Fu, Fan Cheng and Chao Wu

Agronomy 2026, 16(9), 940; https://doi.org/10.3390/agronomy16090940 - 6 May 2026

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Abstract

Lilium, a traditional plant with dual medicinal and ornamental values, is restricted in its industrial development by the low natural propagation rate of bulbs and dependence on imported high-quality germplasm. To address this bottleneck, this study used new lines of LA hybrid [...] Read more.

Lilium, a traditional plant with dual medicinal and ornamental values, is restricted in its industrial development by the low natural propagation rate of bulbs and dependence on imported high-quality germplasm. To address this bottleneck, this study used new lines of LA hybrid lilies and Asiatic hybrid lilies (three cultivars) as experimental materials to establish an efficient and stable tissue culture and rapid propagation system. Key procedures including disinfection of different explants (bulb scales and capsules), adventitious bud induction and proliferation, rooting culture, as well as acclimatization and transplantation were systematically evaluated. The results showed that bulb scales were superior to capsule seeds as explants in tissue culture (contamination rate 9.44%, regeneration rate 11.92%). After disinfection with 75% ethanol combined with 10% sodium hypochlorite, the contamination rate could be controlled at 14.29–21.43%, and the regeneration rate reached 100%. Supplementation with 50 g·L⁻¹ sucrose + 1 mg·L⁻¹ 6-BA + 0.1 mg·L⁻¹ NAA (Treatment TA1), 50 g·L⁻¹ sucrose + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TA4) in MS medium, combined with dark culture, could effectively promote adventitious bud induction, proliferation and bulblet enlargement. For the rooting stage, the optimal media were 1/2 MS + 0.5 g·L⁻¹ activated charcoal + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TB4) or MS + 0.3 g·L⁻¹ activated charcoal + 2 mg·L⁻¹ 6-BA + 0.5 mg·L⁻¹ NAA (Treatment TB1), and the highest rooting rate of ‘Pink Renault’ reached 100%. When plantlets from all three cultivars were combined and acclimatized and transplanted into sterilized peat soil, the overall survival rate was 89.33%. The TOPSIS method was also adopted for comprehensive evaluation to screen out the optimal culture conditions for different varieties. Based on phenotypic observation and physiological index data, ‘Pink Renault’ showed great potential as an excellent propagation germplasm. The integrated and optimized technical system provides a feasible solution for large-scale and industrialized seedling production of medicinal and ornamental lilies, and is of great practical significance for the efficient utilization of germplasm resources and sustainable development of the lily industry. Full article

(This article belongs to the Section Horticultural and Floricultural Crops)

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17 pages, 8045 KB

Open AccessArticle

Yield Prediction of Winter Wheat (Triticum aestivum) Varieties Using UAV-Derived Multispectral Vegetation Indices Across Growth Stages

by Asparuh I. Atanasov and Atanas Z. Atanasov

Agronomy 2026, 16(9), 939; https://doi.org/10.3390/agronomy16090939 - 6 May 2026

Viewed by 623

Abstract

This study investigates the potential for early yield prediction in nine winter wheat (Triticum aestivum) varieties using multispectral data acquired at different growth stages. The data were collected using an unmanned aerial vehicle (UAV) equipped with a multispectral sensor including a [...] Read more.

This study investigates the potential for early yield prediction in nine winter wheat (Triticum aestivum) varieties using multispectral data acquired at different growth stages. The data were collected using an unmanned aerial vehicle (UAV) equipped with a multispectral sensor including a near-infrared (NIR) band and an RGB camera. An assessment of data acquisition and processing accuracy was conducted. The average ground sampling distance (GSD) was 0.42 cm, compared to the pre-flight planned value of 0.54 cm/pixel. The total processing error ranged between 0.34% and 0.45% of a pixel. Five vegetation indices were analyzed, including three NIR-based indices (NDVI, EVI2, and SAVI) and two RGB-based indices (MPRI and MGVRI). The strongest relationships between yield and NIR-based indices were observed on 26 April (spindle phase), with coefficients of determination (R²) ranging from 0.98 to 0.99, while the weakest relationships occurred in late March (R² = 0.75–0.80). In contrast, RGB-based indices showed the strongest correlation in early December (R² = 0.99) and the weakest on 26 April (R² = 0.38–0.40). Regression models for yield prediction were developed based on both groups of vegetation indices. The results demonstrate that the predictive capability of vegetation indices varies significantly across growth stages, highlighting the importance of temporal data selection for accurate yield estimation. Full article

(This article belongs to the Section Precision and Digital Agriculture)

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16 pages, 1211 KB

Open AccessArticle

Combination of Microbial Agent and Bamboo Biochar Decreased the Content of Cd and Changed the Rhizosphere Microbiome in Oryza sativa L.

by Azmat Gyrat, Jinpeng Hu, Yaqi Zhang, Chanyu Zhuang, Xuan Qu, Huijun Zhao, Kun Ma, Xingfu Yan, Xiaodong Ding and Peng Kang

Agronomy 2026, 16(9), 938; https://doi.org/10.3390/agronomy16090938 - 6 May 2026

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Abstract

Cadmium (Cd) contamination in rice paddies poses serious threats to food safety. This study investigated the effects of bamboo biochar, a microbial agent, and their combination on Cd accumulation, soil properties, and rhizosphere microbial communities in the rice cultivar ‘Ning 47’ (Oryza [...] Read more.

Cadmium (Cd) contamination in rice paddies poses serious threats to food safety. This study investigated the effects of bamboo biochar, a microbial agent, and their combination on Cd accumulation, soil properties, and rhizosphere microbial communities in the rice cultivar ‘Ning 47’ (Oryza sativa L.) under Cd stress (20 mg·kg⁻¹). Cd stress significantly reduced plant height, root length, and yield. However, combined treatment with biochar and microbial agent (CdMB) effectively mitigated these effects, reducing Cd content in grains, stems, and roots by 85.98%, 88.66%, and 73.89%, respectively, compared to Cd treatment alone. The CdMB treatment also significantly increased soil organic matter and total nitrogen content while decreasing soil Cd levels by 88.38%. Network analysis identified Flavisolibacter as a keystone taxon under CdMB treatment, indicating enhanced microbial network stability. This also provides a theoretical reference for the management of heavy metal contamination in agricultural soils. By reducing grain Cd contamination and enhancing soil health, this integrated approach addresses key targets of the United Nations Sustainable Development Goals, including SDG 2 (Zero Hunger), SDG 3 (Good Health and Well-being), and SDG 15 (Life on Land). Full article

(This article belongs to the Section Farming Sustainability)

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28 pages, 1063 KB

Open AccessArticle

Enhancing Water Productivity and Forage Yield of Egyptian Clover Through Subirrigation Controlled Drainage and Groundwater Utilisation

by Tarek Alshaal, Nevien Elhawat, Shimaa M. Elmahdy, Ramy M. Khalifa, Safwat Hussein Hatab, Mahmoud M. A. Shabana and Mohamed Kh. El-Ghannam

Agronomy 2026, 16(9), 937; https://doi.org/10.3390/agronomy16090937 - 5 May 2026

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Abstract

Water scarcity is a critical constraint to sustainable agricultural production in arid and semi-arid regions. This study evaluated the effectiveness of subirrigation controlled drainage (SCD) systems in improving water use efficiency, soil conditions, and productivity of Egyptian clover (Trifolium alexandrinum L.) over [...] Read more.

Water scarcity is a critical constraint to sustainable agricultural production in arid and semi-arid regions. This study evaluated the effectiveness of subirrigation controlled drainage (SCD) systems in improving water use efficiency, soil conditions, and productivity of Egyptian clover (Trifolium alexandrinum L.) over two consecutive growing seasons (2022–2024). Three drainage treatments were investigated: subirrigation controlled drainage with water table depths of 0.4 m (SCD-0.4) and 0.8 m (SCD-0.8), and conventional free drainage at 1.2 m (SFD-1.2). The results demonstrated that SCD significantly reduced irrigation water requirements, achieving water savings of up to 27% under SCD-0.4 compared with conventional drainage. The shallow water table enhanced groundwater contribution to crop evapotranspiration, reaching over 40%, which improved soil moisture availability and reduced soil water depletion. Consequently, SCD-0.4 increased fresh and dry biomass yields by approximately 18% and significantly improved water productivity and irrigation water productivity. However, controlled drainage led to increased soil salinity due to reduced leaching, particularly in upper soil layers. Economic analysis revealed that SCD-0.4 achieved the highest net returns and water use profitability. Overall, controlled drainage at shallow depths represents an effective strategy to enhance water productivity, crop yield, and economic efficiency, although long-term salinity management must be considered for sustainable implementation. Full article

(This article belongs to the Special Issue Influence of Irrigation and Water Use on Agronomic Traits of Crops—2nd Edition)

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25 pages, 2117 KB

Open AccessArticle

Genetic Diversity of ‘Candidatus Phytoplasma solani’ in Plant Hosts and Insect Vectors in Winegrowing Regions in Germany

by Barbara Jarausch, Wolfgang Jarausch, Sanela Kugler, Argyroula Tsormpatzidou, Michael Maixner and Anna Markheiser

Agronomy 2026, 16(9), 936; https://doi.org/10.3390/agronomy16090936 - 5 May 2026

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Abstract

The bois noir (BN) disease of grapevines is widespread in German winegrowing regions. It is associated with ‘Candidatus Phytoplasma solani’, which affects not only grapevines but also other wild and cultivated plants. This pathogen has a complex epidemiology including different insect vectors [...] Read more.

The bois noir (BN) disease of grapevines is widespread in German winegrowing regions. It is associated with ‘Candidatus Phytoplasma solani’, which affects not only grapevines but also other wild and cultivated plants. This pathogen has a complex epidemiology including different insect vectors and various host plants. A study was carried out to investigate the genetic variability of ‘Ca. P. solani’ in different winegrowing regions in Germany. Between 2017 and 2023, samples of grapevine, stinging nettle, bindweed, and other herbaceous plants as well as specimens of different planthopper species colonizing viticultural habitats were analyzed for infection with ‘Ca. P. solani’. All positive tested samples were further characterized by multilocus sequence typing (MLST) based on the genes tuf, stamp, secY, and vmp1. The genetic variability was assessed by RFLP analyses of the tuf and vmp1 PCR products, coupled with sequencing of the stamp and secY amplification products. A total of 1274 grapevines, 35 bindweed, and 18 stinging nettle samples were infected with ‘Ca. P. solani’ but also five samples of other weed species. Among the known and putative insect vectors, specimens of Hyalesthes obsoletus, Reptalus spp., and Dictyophara europaea harbored the phytoplasma. In both plants and insects, two genotype combinations were predominantly associated with the classical bindweed and stinging nettle cycle, respectively. The MLST analysis revealed considerable differences between German isolates and data reported from other European regions and new genotype combinations were identified, indicating new host plant–vector associations. Full article

(This article belongs to the Section Agroecology Innovation: Achieving System Resilience)

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15 pages, 1387 KB

Open AccessArticle

Integrating Green Manures and Sweet Sorghum into Sugarcane Rotations Enhances Yield and Sandy-Soil Hydrophysical Properties

by André Araújo do Nascimento, João Henrique Silva da Luz, Mirela Ferneda, Felipe Escorce Furlan, Tamara Qualharello, Gustavo Henrique Gravatim Costa, Fernando Ferrari Putti and Raúl Andres Martinez Uribe

Agronomy 2026, 16(9), 935; https://doi.org/10.3390/agronomy16090935 - 5 May 2026

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Abstract

Sugarcane is the leading feedstock for bioethanol in Brazil and worldwide, but its continuous cultivation can degrade soil through nutrient depletion and compaction. Integrating green manures such as Crotalaria and pigeon pea into rotations offers a sustainable way to improve soil structure, water [...] Read more.

Sugarcane is the leading feedstock for bioethanol in Brazil and worldwide, but its continuous cultivation can degrade soil through nutrient depletion and compaction. Integrating green manures such as Crotalaria and pigeon pea into rotations offers a sustainable way to improve soil structure, water infiltration, and nutrient cycling. When combined with sweet sorghum as a complementary crop, these species can mitigate soil physical constraints and strengthen the resilience of sugar–energy systems under rainfed conditions. This three-year field experiment evaluated the effects of green manure and sweet sorghum rotations on sugarcane yield and sandy-soil physical attributes. The treatments were arranged in a 3 × 2 factorial design with randomized blocks, including two green manures (Crotalaria and pigeon pea) and a fallow control, each combined with or without sweet sorghum rotation. Biometric traits and yields were measured for all crops, and soil physical properties were assessed after the sugarcane cycle. Green manure significantly increased the stalk yield and dry matter of both sweet sorghum and sugarcane. In sugarcane, rotations with Crotalaria and pigeon pea enhanced stalk and dry matter yields by up to 18%, while the highest increase (31%) occurred under the sweet sorghum rotation. Furthermore, green manures improved sandy-soil water retention, increased infiltration rates, and reduced penetration resistance. These results demonstrate that legume–sorghum rotations are an effective and low-input strategy to enhance crop yield and sandy-soil physical properties, contributing to more sustainable bioenergy production under tropical rainfed conditions. Full article

(This article belongs to the Special Issue Sustainable Cropping Systems and Biomasses for Energy and Biorefinery Applications)

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20 pages, 14067 KB

Open AccessArticle

Uncovering Stable Genetic Loci for Sustainable Pea (Pisum sativum L.) Production Through Genome-Wide Association Mapping

by Alibek Zatybekov, Evgeniy Ten, Irina Oshergina, Sergey Radul, Akerke Amalova, Saule Abugalieva and Yerlan Turuspekov

Agronomy 2026, 16(9), 934; https://doi.org/10.3390/agronomy16090934 - 4 May 2026

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Abstract

A comprehensive evaluation of phenotypic diversity, genetic structure, and marker–trait associations was conducted in a pea (Pisum sativum L.) collection of 184 accessions, using multi-environment field trials and genome-wide SNP data. Agronomic traits were assessed using best linear unbiased estimates, and statistical [...] Read more.

A comprehensive evaluation of phenotypic diversity, genetic structure, and marker–trait associations was conducted in a pea (Pisum sativum L.) collection of 184 accessions, using multi-environment field trials and genome-wide SNP data. Agronomic traits were assessed using best linear unbiased estimates, and statistical analyses included correlation, analysis of variance, heritability estimation, population structure, linkage disequilibrium, and genome-wide association study of 10,289 SNP markers. Phenological traits showed low variability, with flowering and maturity averaging 36.08 and 79.19 days (coefficient of variation of 6.17% and 3.79%, respectively), whereas yield-related traits varied more widely, with the number of pods per plant showing a coefficient of variation of 26.14%. Strong correlations were observed between plant height and height of the lowest pod attachment (r = 0.89, p < 0.001), while moderate positive correlations were found between flowering and maturity time (r = 0.43, p < 0.001) and between number of pods per plant and plant height (r = 0.44, p < 0.001); meanwhile, thousand seed weight exhibited significant negative correlation number of pods per plant (r = −0.42, p < 0.001). Heritability was highest for plant height (H² = 0.925), height of the lowest pod attachment (H² = 0.889), and thousand seed weight (H² = 0.883), while yield showed lower heritability (H² = 0.672) and strong environmental influence. Linkage disequilibrium decay was 1.78 Mb at r² = 0.2. GWAS identified 163 quantitative trait loci, including 19 stable loci, with strong effects such as −19.27 cm for q.PH.5-1 and +24.62 g for q.TSW.4-2. Candidate genes associated with key biological processes were identified, thereby enhancing understanding of the genetic control of traits. Full article

(This article belongs to the Special Issue Crop Genomics and Omics for Future Food Security)

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18 pages, 1091 KB

Open AccessArticle

Labile Carbon Additions Reduce Soil Nitrate but Can Increase Maize Fertilizer N Needs

by Stephen W. Potter, John E. Sawyer and Marshall D. McDaniel

Agronomy 2026, 16(9), 933; https://doi.org/10.3390/agronomy16090933 - 4 May 2026

Viewed by 275

Abstract

Winter cover crops (WCCs) are effective at reducing N losses from temperate agroecosystems. Although extensive research on WCCs has demonstrated numerous benefits, overall adoption rates in the Midwest U.S. remain low. We evaluated an alternative to WCC’s ability to reduce nitrate (NO₃ [...] Read more.

Winter cover crops (WCCs) are effective at reducing N losses from temperate agroecosystems. Although extensive research on WCCs has demonstrated numerous benefits, overall adoption rates in the Midwest U.S. remain low. We evaluated an alternative to WCC’s ability to reduce nitrate (NO₃⁻)-N leaching; that is, adding an inexpensive, easy-to-apply, form of labile carbon (C) as a soil amendment intended to immobilize N and mitigate leaching. In the autumn in a typical maize–soybean rotation, we added crude glycerol (a C-rich, biodiesel byproduct) and hypothesized that glycerol carbon (C_glyc) would immobilize N and have no effect on crop growth. More specifically, C_glyc was broadcast applied at three rates (0, 216, and 866 kg C ha⁻¹ y⁻¹) and combined factorially with six spring-applied fertilizer N rates (0, 56, 112, 168, 224, and 280 kg N ha⁻¹) at two sites. In response, we measured: soil profile NO₃⁻-N, leached NO₃⁻-N, crop health (via SPAD), yield, and maize agronomic optimum N rate (AONR). C_glyc reduced spring soil profile NO₃⁻-N by 14–24% across site-years, but had highly variable and non-significant effects on NO₃⁻-N leaching. C_glyc had an inconsistent impact on crop SPAD and yield, with C_glyc increasing AONR by ~63 kg N ha⁻¹ (or 31–40%) at one of two sites. Our results show promise for using labile C as a “liquid cover crop” soil amendment. Future studies should explore greater labile C application rates and alternate application timing in order to fine-tune the balance between environmental benefits and crop productivity. Full article

(This article belongs to the Special Issue Enhancing Nitrogen Use Efficiency in Major Crops: Mechanisms, Management, and Synergies with Yield Formation)

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22 pages, 4925 KB

Open AccessArticle

Tomato Ripeness Detection Model Based on Improved RT-DETR Lightweight Model

by Guoliang Yang, Dali Weng, Zhiteng Li and Yonggan Wu

Agronomy 2026, 16(9), 932; https://doi.org/10.3390/agronomy16090932 - 4 May 2026

Viewed by 337

Abstract

Accurate tomato ripeness detection is crucial for automated harvesting; however, complex greenhouse environments—characterized by dynamic light interference, foliage occlusion, and dense fruit overlapping—severely hinder detection performance and lead to frequent misdetections. This study aims to develop a high-precision, lightweight detection model that simultaneously [...] Read more.

Accurate tomato ripeness detection is crucial for automated harvesting; however, complex greenhouse environments—characterized by dynamic light interference, foliage occlusion, and dense fruit overlapping—severely hinder detection performance and lead to frequent misdetections. This study aims to develop a high-precision, lightweight detection model that simultaneously addresses these three core challenges, thereby providing a technically deployable algorithmic foundation for resource-constrained agricultural edge devices. To this end, we propose CFD-DETR, a lightweight tomato ripeness detection model based on the RT-DETR architecture. The model incorporates a CAEfficientViT backbone for the lightweight extraction of multi-scale color and texture features. Furthermore, a Focused Efficient Additive Attention (FEAA) mechanism is integrated to capture fine-grained local ripening traits with minimal computational overhead. During feature reconstruction, a Deep Dynamic Upsampling (DwDySample) operator is utilized to preserve semantic integrity. Additionally, we designed the Wise-SIoU loss function, which dynamically penalizes low-quality samples to enhance boundary fitting and robustness against background noise. Experimental evaluations demonstrate that CFD-DETR achieves 90.2% mAP@0.5, outperforming the baseline model by 2.1 percentage points while significantly reducing the parameter count and computational complexity by 47.2% and 52.5%, respectively. Cross-dataset validation on the publicly available Laboro Tomato and RaUTD datasets confirms the model’s superior generalization capabilities. Overall, CFD-DETR provides a highly efficient and robust solution for real-time agricultural robotics. Full article

(This article belongs to the Topic Digital Agriculture, Smart Farming and Crop Monitoring)

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21 pages, 3800 KB

Open AccessArticle

Metagenomic Study on the Association Between Rhizosphere Soil Microbial Communities and Cold Tolerance in Maize

by Tao Yu, Jianguo Zhang, Xuena Ma, Shiliang Cao, Wenyue Li and Gengbin Yang

Agronomy 2026, 16(9), 931; https://doi.org/10.3390/agronomy16090931 - 3 May 2026

Viewed by 514

Abstract

To elucidate the mechanisms by which the rhizosphere microbial community influences cold tolerance in maize, this study employed the metagenomic technology to systematically analyze the community composition, functional characteristics, and their association with host cold tolerance in the rhizosphere of maize genotypes with [...] Read more.

To elucidate the mechanisms by which the rhizosphere microbial community influences cold tolerance in maize, this study employed the metagenomic technology to systematically analyze the community composition, functional characteristics, and their association with host cold tolerance in the rhizosphere of maize genotypes with different cold tolerance (cold-tolerant material B144 and cold-sensitive material Q319, among others) (n = 3 biological replicates per genotype). The results revealed that the rhizosphere microbial community of the cold-tolerant genotype B144 exhibited higher species diversity and more complex genomic features. LEfSe analysis indicated that the rhizosphere soil microbiota of B144 was significantly enriched in two major phyla, Firmicutes and Actinobacteria, as well as microbial taxa with stress tolerance potential, such as the Bacillus and Streptomyces. Further functional analysis revealed that the microbial community was specifically enriched in metabolic pathways related to glycan biosynthesis and metabolism, as well as coenzyme and vitamin metabolism. We hypothesize that the physiological stability of maize under low temperatures can be enhanced through mechanisms such as the synthesis of extracellular polysaccharides to reduce the freezing point and the provision of vitamins and antioxidant substances. In contrast, the rhizosphere microorganisms of the cold-sensitive material Q319 were more enriched in basic metabolic functions. The present study elucidates the pivotal mechanisms by which rhizosphere microorganisms facilitate maize resistance to low-temperature stress from a functional perspective. This provides theoretical support and new strategies for enhancing crop stress resistance by regulating the rhizosphere microbiome. Full article

(This article belongs to the Special Issue Plant Stress Tolerance: From Genetic Mechanism to Cultivation Methods)

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35 pages, 2827 KB

Open AccessArticle

Environmental Implications and Risk Assessment of Pesticide Residues in Soils and Water in One of the Most Important Agricultural Regions in Niger

by Djamilou Gabèye, Martin Wiehle and Abdourahamane Tankari Dan Badjo

Agronomy 2026, 16(9), 930; https://doi.org/10.3390/agronomy16090930 - 3 May 2026

Viewed by 481

Abstract

In sub-Saharan Africa, intensive pesticide use in irrigated agriculture is threatening the quality of soil, water bodies and ecosystem services, yet integrated risk assessments remain limited. This study evaluated the environmental implications and risks of pesticide residues in soils (0–20 cm; n = [...] Read more.

In sub-Saharan Africa, intensive pesticide use in irrigated agriculture is threatening the quality of soil, water bodies and ecosystem services, yet integrated risk assessments remain limited. This study evaluated the environmental implications and risks of pesticide residues in soils (0–20 cm; n = 15) and irrigation water (n = 15) from off-season irrigation area of the Goulbi Maradi Valley, Niger. Twelve commonly used pesticides in Djiratawa, Maradi 3 and Tibiri, were quantified by High-Performance Liquid Chromatography with Variable Wavelength Detector (HPLC-VWD), revealing Tibiri as a contamination hotspot, where the total pesticide residues in soil and irrigation water reached 6.4 and 19.7 times the respective European Union soil and drinking water benchmarks, dominated by Cypermethrin, Emamectin benzoate and Chlorpyrifos ethyl in soils, and Emamectin benzoate and Dichlorvos in water. Multivariate analysis showed that soil particle size, particularly higher clay content, controlled the retention of strongly sorbing compounds, while pH and salinity governed the occurrence of more soluble residues in irrigation water. While non-carcinogenic risks for Adults and Children via soil and water exposure were acceptable (Hazard Quotient and Hazard Index < 1), ecological risks were unacceptable, with Folsomia candida and Daphnia magna the most affected organisms, driven by Emamectin benzoate (Toxicity Exposure Ratio < 2). Priority actions include phasing out Dichlorvos and Paraquat dichloride, tightening controls on Emamectin benzoate and expanding food-chain monitoring, particularly in vegetables and fish, to support multi-trophic risk assessment and safer irrigation management. Full article

(This article belongs to the Section Pest and Disease Management)

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26 pages, 1483 KB

Open AccessArticle

Comparison of Polyphenol Content and Antioxidant Activity in Leaves of Seven Avocado (Persea americana Mill.) Cultivars

by Samuel Valdebenito, Marcela Escobar, Ricardo Cautín, Juan Vidal, Martina Ruiz, Benjamín Astudillo, Ignacia Hernández and Patricia Peñaloza

Agronomy 2026, 16(9), 929; https://doi.org/10.3390/agronomy16090929 - 3 May 2026

Viewed by 851

Abstract

Leaves of Persea americana Mill. are a potential source of bioactive compounds; however, current knowledge is limited by the lack of studies that simultaneously evaluate multiple cultivars and leaf developmental stages under comparable field conditions, as well as by inconsistent sampling criteria across [...] Read more.

Leaves of Persea americana Mill. are a potential source of bioactive compounds; however, current knowledge is limited by the lack of studies that simultaneously evaluate multiple cultivars and leaf developmental stages under comparable field conditions, as well as by inconsistent sampling criteria across studies. This study aimed to assess how leaf developmental stage and cultivar influence phenolic composition and antioxidant activity in avocado leaves grown under field conditions. Total polyphenols, total flavonoids, quercetin-3-O-galactoside, and chlorogenic acid were selected as representative compounds due to their relevance in plant defense and antioxidant capacity. These compounds, together with antioxidant activity, were quantified in young expanding (E1) and fully expanded (E6) leaves of seven avocado cultivars using spectrophotometric methods and HPLC-DAD, and analyzed through two-way ANOVA. Results showed that leaf developmental stage significantly influenced flavonoid content, chlorogenic acid, and antioxidant activity, which were consistently higher in E1 leaves. In contrast, total polyphenol content exhibited cultivar-dependent responses, increasing toward E6 only in specific genotypes, while quercetin-3-O-galactoside remained stable across developmental stages but varied among cultivars. Overall, the results indicate that phenolic metabolism in avocado leaves is regulated by both developmental stage and genotype in a compound-specific manner, with no consistent pattern associated with racial background. From a practical perspective, these findings provide a basis for selecting leaf material according to the intended application: young leaves for higher antioxidant capacity and flavonoid content, and specific cultivars at advanced stages for higher total polyphenol accumulation, supporting the functional valorization of avocado leaves as a source of bioactive compounds. Full article

(This article belongs to the Special Issue Genotype × Environment Interactions in Crop Production—2nd Edition)

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22 pages, 2726 KB

Open AccessArticle

Exogenous Abscisic Acid Modulates Physiological and Sugar Metabolic Responses to Alleviate Low-Light Injury in Cherry Tomato

by Xin Yang, Jun Nie, Yu Yuan, Yuming Xie, Liangliang Shi and Yanhong Li

Agronomy 2026, 16(9), 928; https://doi.org/10.3390/agronomy16090928 - 2 May 2026

Viewed by 431

Abstract

Low-light (LL) stress is a major abiotic limiting factor in protected cherry tomato production, adversely affecting vegetative growth, inducing oxidative damage, and disrupting fruit sugar metabolism. To clarify the regulatory role of exogenous abscisic acid (ABA) in mitigating LL stress, we examined the [...] Read more.

Low-light (LL) stress is a major abiotic limiting factor in protected cherry tomato production, adversely affecting vegetative growth, inducing oxidative damage, and disrupting fruit sugar metabolism. To clarify the regulatory role of exogenous abscisic acid (ABA) in mitigating LL stress, we examined the effects of varying ABA concentrations on plant growth, antioxidant capacity, and fruit sugar metabolism in cherry tomatoes under low-light conditions. A two-factor randomized complete block design, with two light regimes—normal light (NL, 100% natural sunlight) and low light (LL, 25% natural sunlight)—and three ABA concentrations (CK: 0 mg·L⁻¹, T1: 10 mg·L⁻¹, T2: 20 mg·L⁻¹). Fruits were sampled at three typical ripening stages (green mature, breaker, and red ripe) to evaluate vegetative and reproductive physiological responses. The results showed that exogenous ABA application effectively suppressed LL-induced excessive stem elongation and alleviated LL-caused reductions in stem diameter and biomass accumulation. ABA treatment significantly increased peroxidase (POD) activity and reduced malondialdehyde (MDA) and hydrogen peroxide (H₂O₂) accumulation, thereby relieving LL-triggered oxidative damage. In addition, ABA regulated key sugar-metabolizing enzymes (soluble acid invertase (SAI), sucrose synthase (SS), sucrose phosphate synthase (SPS), and amylase (Amy)) and the transcript levels of related functional genes (HXK1, SPS, SS, AI), thereby mediating stage-dependent fruit sugar metabolism under LL stress. In conclusion, exogenous ABA effectively modulates vegetative growth, antioxidant homeostasis, and stage-specific fruit sugar metabolism, ultimately alleviating low-light stress damage in cherry tomato. Among the tested treatments, 20 mg·L⁻¹ ABA exhibited the most pronounced mitigation effects, which can be recommended as an optimal foliar application concentration for cherry tomato cultivation in low-light protected facilities. Full article

(This article belongs to the Section Horticultural and Floricultural Crops)

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12 pages, 864 KB

Open AccessArticle

Biomass Production and Nitrogen Accumulation of Summer Cover Crop Mixtures Under Two Sowing Dates in a Cool Temperate Climate

by Kristo Tikk, Liina Talgre, Karli Sepp, Liina Edesi, Karin Kauer, Viacheslav Eremeev and Merili Toom

Agronomy 2026, 16(9), 927; https://doi.org/10.3390/agronomy16090927 - 2 May 2026

Viewed by 304

Abstract

Summer cover crops can improve soil fertility and contribute to nitrogen (N) supply in temperate cropping systems, yet the effects of mixture composition and sowing timing remain insufficiently documented. This study evaluated biomass production and N accumulation of five multispecies cover crop mixtures [...] Read more.

Summer cover crops can improve soil fertility and contribute to nitrogen (N) supply in temperate cropping systems, yet the effects of mixture composition and sowing timing remain insufficiently documented. This study evaluated biomass production and N accumulation of five multispecies cover crop mixtures grown in Estonia during 2024–2025 under two sowing dates per year. Aboveground biomass, botanical composition, and carbon (C) and nitrogen concentrations were measured to assess productivity, species contributions, and residue quality. Earlier sowing was generally associated with higher biomass and N accumulation, with first-sown mixtures producing, on average, 38.7% more biomass than later-sown mixtures. Mixture performance was strongly shaped by species composition and competitive hierarchies. Total N accumulation of the cover crop mixtures ranged from 42 to 275 kg N ha⁻¹ depending on mixture composition and sowing time, with mixtures dominated by common vetch (Vicia sativa L.) achieving the highest values. Oat (Avena sativa) dominated and contributed substantially to biomass in mixtures lacking competitive legumes, whereas sunflower (Helianthus annuus) and maize (Zea mays) performed less well under delayed sowing. Low-growing species such as Persian clover (Trifolium resupinatum) produced little biomass when grown with highly competitive species. Legumes exhibited lower C:N ratios than non-legumes, while mixture-level values remained moderate, suggesting residue quality with potential for favourable decomposition and nutrient release in summer cover crop systems under temperate conditions. Full article

(This article belongs to the Special Issue Cover Crops Grown Alone and in Consortia: Biomass Production, Nutrient Cycling of Residues and Relationships with Soil and Crop Productivity)

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20 pages, 12772 KB

Open AccessArticle

Molecular Mechanism by Which OsSUT2 Regulates Chalkiness Formation in Rice Grains

by Dongping Yao, Xiaoqiao Yin, Dengkui Liu, Fudie Meng, Chunfen Long, Yingge Li, Xuemei Zhong and Bin Bai

Agronomy 2026, 16(9), 926; https://doi.org/10.3390/agronomy16090926 - 2 May 2026

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Abstract

Rice chalkiness is a key constraint in breeding high-quality rice, and unbalanced sucrose transport and starch metabolism are its primary causes. To clarify the molecular mechanism by which OsSUT2 regulates rice grain chalkiness formation, the rice cultivar TP309 was used as material, and [...] Read more.

Rice chalkiness is a key constraint in breeding high-quality rice, and unbalanced sucrose transport and starch metabolism are its primary causes. To clarify the molecular mechanism by which OsSUT2 regulates rice grain chalkiness formation, the rice cultivar TP309 was used as material, and ossut2 homozygous mutants were generated via CRISPR/Cas9. Systematic studies were performed using genetic complementation, phenotypic identification, cytological observation, transcriptome sequencing, and haplotype analysis. The results show that loss of OsSUT2 function significantly increased grain chalkiness, deteriorated agronomic traits, induced carbon assimilate accumulation in leaves, blocked sugar transport and starch synthesis in grains, and destroyed starch fine structure; the mutant phenotype was largely restored by functional complementation with wild-type OsSUT2. OsSUT2 was expressed in both source and sink organs, with the strongest inhibition detected in the panicles. Mutation of OsSUT2 disrupted sucrose and starch metabolic pathways. Three main haplotypes of OsSUT2 were identified in natural populations, with significant indica–japonica differentiation. OsSUT2 is confirmed as a key regulator of rice chalkiness, providing gene resources and theoretical support for rice quality improvement. Full article

(This article belongs to the Special Issue Genetics and Breeding of Field Crops in the 21st Century—2nd Edition)

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15 pages, 6072 KB

Open AccessArticle

Long-Term Cotton Straw Returning May Increase Soil Secondary Salinization Risk in Some Xinjiang Cotton Fields: Evidence of Threshold-like Responses and Spatial Heterogeneity

by Changxue Wu, Junxiao Zhang, Xiangwen Xie, Renna Sa, Yeshan Zhang and Yongmei Xu

Agronomy 2026, 16(9), 925; https://doi.org/10.3390/agronomy16090925 - 2 May 2026

Viewed by 366

Abstract

Background: Cotton straw returning (CSR) is widely implemented in Xinjiang to achieve in situ residue utilization and ameliorate saline–alkali soils. However, its long-term efficacy in improving soil fertility without inducing secondary salinization remains poorly understood. Methods: This study evaluated the effects of different [...] Read more.

Background: Cotton straw returning (CSR) is widely implemented in Xinjiang to achieve in situ residue utilization and ameliorate saline–alkali soils. However, its long-term efficacy in improving soil fertility without inducing secondary salinization remains poorly understood. Methods: This study evaluated the effects of different CSR durations on soil physicochemical properties and cotton yield across four major cotton-growing regions of Xinjiang, China (Shawan, Wusu, Manas, and Shihezi). Cotton fields with different CSR durations were classified into four treatments: CK (0 years), T1 (5 years), T2 (10 years), and T3 (20 years). Soil bulk density (BD), exchangeable sodium percentage (ESP), and key soil fertility indicators were measured to assess comprehensive soil fertility and soil secondary salinization risk. Results: CSR generally enhanced soil physicochemical properties, but responses exhibited spatial heterogeneity. Soil BD decreased in Wusu and Shihezi but showed temporary increases in Manas and Shawan. SFI increased continuously with CSR duration in Shawan and Wusu, whereas it peaked at around 10 years in Shihezi and Manas. Cotton yield was highest under the 20-year treatment in Manas but peaked under the 10-year treatment in the other regions. Notably, prolonged CSR (>10 years) elevated ESP in some areas, suggesting an increased risk of secondary salinization. Conclusions: A CSR duration of roughly 10 years appears optimal for balancing soil amelioration and salinity control in saline–alkali cotton fields of Xinjiang. Full article

(This article belongs to the Section Soil and Plant Nutrition)

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23 pages, 5072 KB

Open AccessArticle

Harnessing Cooperative Bacterial Consortia to Enhance Agronomic Performance, Yield, and Grain Quality of Lupinus luteus Under Field-Based Drought Conditions

by Macarena Barra-Jiménez, Karen Vergara, Paulina Molinet, Milko A. Jorquera, Joaquin Rilling, Grace Armijo-Godoy and Jacquelinne J. Acuña

Agronomy 2026, 16(9), 924; https://doi.org/10.3390/agronomy16090924 - 1 May 2026

Viewed by 457

Abstract

The use of microbial consortia has emerged as a promising strategy to improve crop performance under abiotic stress, although their effectiveness under field conditions remains variable. Here, we evaluated whether plant growth-promoting (PGP) bacterial consortia assembled based on synergistic PGP traits can improve [...] Read more.

The use of microbial consortia has emerged as a promising strategy to improve crop performance under abiotic stress, although their effectiveness under field conditions remains variable. Here, we evaluated whether plant growth-promoting (PGP) bacterial consortia assembled based on synergistic PGP traits can improve physiological performance, yield, and grain quality of yellow lupine (Lupinus luteus L.) under field-based drought conditions. A semi-controlled, field-based pot experiment was conducted under contrasting water regimes (irrigated and drought) to evaluate four rhizobacterial strains (Microbacterium sp. S13.2, Variovorax sp. S14.7, Bacillus sp. S31, and Lysinibacillus sp. S34), assembled into four consortia: two characterized by high (C1 and C2) and two by low (C3 and C4) auxin production and ACC deaminase activity, along with an uninoculated control. Physiological responses were monitored across phenological stages through stomatal conductance and photosynthetic pigments, while agronomic traits, yield components, and grain quality were assessed at harvest. Inoculation effects were stage-dependent and became more evident under drought conditions. Consortia C1(Microbacterium sp. S13.2 + Variovorax sp. S14.7) and C2 (Bacillus sp. S31 + Lysinibacillus sp. S34) consistently improved biomass accumulation, seed number, and grain yield compared to the uninoculated control, whereas C3 (Lysinibacillus sp. S34 + Variovorax sp. S14.7) and C4 (Bacillus sp. S31 + Variovorax sp. S14.7) showed limited or neutral effects. Multivariate analysis indicated distinct performance strategies, with C1 associated with higher productivity and C2 with improved yield stability under drought. Grain quality parameters remained stable across treatments. These results show that cooperative microbial consortia can improve lupine performance under water-limited conditions, and their effectiveness depends on the functional interactions among consortium members. Full article

(This article belongs to the Topic Microbe-Induced Abiotic Stress Alleviation in Plants, 2nd Edition)

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19 pages, 7010 KB

Open AccessArticle

Fast-Tracking Trait Combination in Triticale Through Doubled Haploid Technology

by Sue Broughton, Marieclaire Castello, Yong Han, Richard Bennett, Manisha Shankar, Ryan Varischetti and Daniel Real

Agronomy 2026, 16(9), 923; https://doi.org/10.3390/agronomy16090923 - 1 May 2026

Viewed by 452

Abstract

This study aimed to develop an anther culture protocol for triticale (×Triticosecale Wittmack) and generate a doubled haploid (DH) population combining awnlessness and stripe rust resistance. A wheat anther culture protocol was evaluated on nine triticale varieties, with and without Trichostatin A [...] Read more.

This study aimed to develop an anther culture protocol for triticale (×Triticosecale Wittmack) and generate a doubled haploid (DH) population combining awnlessness and stripe rust resistance. A wheat anther culture protocol was evaluated on nine triticale varieties, with and without Trichostatin A (TSA), and tested on parental genotypes—an awned, stripe rust–resistant breeding line (AT-45) and an awnless variety (‘1143’)—as well as on ten F₁ plants derived from crosses between AT-45 and ‘1143’. Plant regeneration varied widely among varieties, ranging from 0.8 to 39.7 green plants per 30 anthers (1.6–80 per spike), with an overall mean of 9.9 (20 per spike). TSA did not significantly improve green plant production in this study, though further optimisation of the application method may be warranted. An average of 17.4 green plants per spike was obtained from the F₁ plants, and 1130 regenerant plants were grown to maturity, with a mean spontaneous chromosome doubling rate of 42.5%. A total of 480 DH lines were harvested, comprising 250 awned, 60 reduced awn, and 170 awnless lines. Awned and reduced awn lines were discarded, and 114 awnless lines were advanced for field evaluation of stripe rust resistance and agronomic traits. These results establish an effective anther culture system for DH production in triticale and demonstrate the potential of DH technology to accelerate the development of resilient, high-performing varieties. Full article

(This article belongs to the Section Crop Breeding and Genetics)

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25 pages, 4439 KB

Open AccessArticle

Monitoring Crop Structure and Moisture Using GNSS Interferometric Reflectometry Based on SNR Modeling

by Samuele De Petris and Enrico Borgogno-Mondino

Agronomy 2026, 16(9), 922; https://doi.org/10.3390/agronomy16090922 - 1 May 2026

Viewed by 531

Abstract

This study aims to evaluate the potential of Global Navigation Satellite System Interferometric Reflectometry (GNSS-IR) based on signal-to-noise ratio (SNR) analysis for monitoring crop structure and moisture. Data were collected using a GNSS antenna placed within an experimental meadow located in NW Italy. [...] Read more.

This study aims to evaluate the potential of Global Navigation Satellite System Interferometric Reflectometry (GNSS-IR) based on signal-to-noise ratio (SNR) analysis for monitoring crop structure and moisture. Data were collected using a GNSS antenna placed within an experimental meadow located in NW Italy. GNSS-IR exploits the interference between direct and ground-reflected signals to derive physical parameters such as the vegetation phase center height and soil moisture. In this work, by analyzing and modeling the oscillations in SNR time series, the sensitivity to crop growth dynamics was assessed. Vegetation height and dielectric parameters were compared against corresponding ground-surveyed values collected using a ruler and buried soil moisture sensors. Results suggest that GNSS-IR can detect canopy height with a high degree of consistency (Pearson’s r = 0.89, MAPE = 18%). Results also show that changes in the amplitude and phase of the interference pattern are sensitive to biomass density and dielectric properties of the reflecting surface (r = −0.81 and r = 0.86 respectively). GNSS-IR observables were analyzed across four representative measurement campaigns capturing distinct seasonal stages of meadow development. Despite the limited temporal sampling (n = 4), the selected observations correspond to contrasting vegetation and soil moisture conditions, allowing the identification of systematic variations in crop biophysical properties. These findings open promising perspectives for the development of innovative monitoring strategies in precision agriculture, leveraging existing GNSS infrastructure to obtain key biophysical parameters with minimal additional equipment and operational complexity. Full article

(This article belongs to the Special Issue Smart Farming Technologies for Sustainable Agriculture—2nd Edition)

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14 pages, 6150 KB

Open AccessArticle

TaNSUN2-Mediated m⁵C Modification of TaTHI2 Modulates Antiviral Immunity Against Chinese Wheat Mosaic Virus

by Liwen Chen, Meichen Zhang, Yulun Wu, Lixiao Feng, Ying Liu, Jiaqian Liu, Jian Yang and Yaoyao Jiang

Agronomy 2026, 16(9), 921; https://doi.org/10.3390/agronomy16090921 - 30 Apr 2026

Viewed by 288

Abstract

Although RNA cytosine-5 methylation (m⁵C) is an important post-transcriptional regulatory mechanism, its contribution to plant antiviral immunity remains unclear. In this study, we identified Thiamine thiazole synthase 2 (TaTHI2) as a host mRNA target of the wheat m⁵C methyltransferase [...] Read more.

Although RNA cytosine-5 methylation (m⁵C) is an important post-transcriptional regulatory mechanism, its contribution to plant antiviral immunity remains unclear. In this study, we identified Thiamine thiazole synthase 2 (TaTHI2) as a host mRNA target of the wheat m⁵C methyltransferase TaNSUN2 during infection by Chinese wheat mosaic virus (CWMV), a soil-borne virus that poses a major threat to wheat production. TaNSUN2 contributes to the m⁵C modification of TaTHI2 transcripts, enhancing mRNA stability and sustaining TaTHI2 accumulation. The disruption of a key m⁵C site markedly reduced methylation, weakened TaNSUN2–RNA binding, and accelerated transcript decay, leading to the compromised production of reactive oxygen species (ROS) and increased viral infection. Mechanistically, the TaNSUN2-dependent m⁵C modification stabilized TaTHI2 mRNA, thereby promoting ROS-mediated antiviral defense. Collectively, our results establish the m⁵C modification of TaTHI2 mRNA as a critical post-transcriptional control point in CWMV resistance and highlight TaNSUN2-dependent RNA methylation as an integral component of host antiviral immunity. Full article

(This article belongs to the Special Issue Regulatory Networks in Plant Response to Pathogens)

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17 pages, 1394 KB

Open AccessArticle

Evaluation and Selection of Spring Wheat Cultivars for Adaptation to the Wheat–Soybean Double-Cropping System

by Dong Deng, Yuning Huang, Weide Ge, Yingjie Wang, Yan Zhuang, Tao Li, Renfeng Xue and Ming Feng

Agronomy 2026, 16(9), 920; https://doi.org/10.3390/agronomy16090920 - 30 Apr 2026

Cited by 1 | Viewed by 463

Abstract

The wheat–soybean double-cropping system enables the continuous production of preceding and succeeding crops within the same growing season, providing an important approach for improving arable land-use efficiency, increasing output per unit area, and optimizing cropping structure. In Liaoning Province, where thermal resources and [...] Read more.

The wheat–soybean double-cropping system enables the continuous production of preceding and succeeding crops within the same growing season, providing an important approach for improving arable land-use efficiency, increasing output per unit area, and optimizing cropping structure. In Liaoning Province, where thermal resources and the frost-free period are relatively limited, this system places high requirements on the growth duration, yield stability, and succession compatibility of the preceding wheat crop with the succeeding soybean crop. To identify spring wheat cultivars suitable for this system, field trials were conducted from 2021 to 2023, using three representative ecological regions of Liaoning Province. Ten widely grown spring wheat cultivars were evaluated for major agronomic traits, grain quality, and disease resistance, and their stability and system adaptability were analyzed using a mixed linear model, GGE biplot analysis, and TOPSIS. The results showed clear differences among cultivars in growth duration, wheat yield, and succeeding soybean yield. Liaochun 33 and Liaochun 18 had relatively short growth durations of 78–84 days and 79–83 days, respectively, and showed favorable performance in wheat yield, succeeding soybean yield, and stability. Combined with grain quality, disease resistance, and TOPSIS-based comprehensive evaluation, Liaochun 33 showed the best overall performance, while Liaochun 18 also exhibited strong system adaptability. Overall, cultivar selection for the wheat–soybean double-cropping system in Liaoning Province should shift from single wheat-yield evaluation to overall system-benefit evaluation. Liaochun 33 and Liaochun 18 can be recommended as preferred spring wheat cultivars for this cropping system. Full article

(This article belongs to the Section Innovative Cropping Systems)

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5 pages, 155 KB

Open AccessEditorial

Editorial: Facility Agriculture Robots and Autonomous Unmanned Management for Crops I

by Wei Ma

Agronomy 2026, 16(9), 919; https://doi.org/10.3390/agronomy16090919 - 30 Apr 2026

Viewed by 260

Abstract

Due to both continued global population growth and an accelerating aging trend in the rural labor force, agricultural production is facing unprecedented challenges [...] Full article

(This article belongs to the Special Issue Facility Agriculture Robots and Autonomous Unmanned Management for Crops)

19 pages, 5961 KB

Open AccessArticle

Long-Term No-Tillage and Straw Mulching Improves Aggregate Stability by Increasing Mineral-Associated Organic Carbon in Microaggregates

by Yidan He, Jiayu Qin, Yong Zhou, Ligeng Jiang, Yanli Chen, Hang Wu, Shihong Xu and Pengli Yuan

Agronomy 2026, 16(9), 918; https://doi.org/10.3390/agronomy16090918 - 30 Apr 2026

Viewed by 339

Abstract

Straw and no-tillage management, as important practices in conservation agriculture, have the potential to improve soil structure. However, their effects on the aggregate stability of soil and on active organic carbon pools in paddy fields are unclear. To investigate how different tillage and [...] Read more.

Straw and no-tillage management, as important practices in conservation agriculture, have the potential to improve soil structure. However, their effects on the aggregate stability of soil and on active organic carbon pools in paddy fields are unclear. To investigate how different tillage and straw management practices affect soil properties, this study drew on a 15-year long-term experiment conducted in a double-cropped rice region in South China. It systematically compared four treatments: no-tillage (NT), conventional tillage (CT), conventional tillage with incorporated straw (CT-SR), and no-tillage with straw mulch (NT-SMR)—in terms of their effects on the distribution and stability of mechanical and water-stable aggregates, as well as the distribution of particulate organic carbon (POC) and mineral-associated organic carbon (MAOC) across various aggregate size fractions. The results showed that: (1) Relative to the CT, NT, and CT-SR treatments, NT-SMR significantly enhanced soil structure, as evidenced by a higher percentage of large aggregates (>0.25 mm) and improved aggregate stability. (2) NT-SMR consistently increased soil organic carbon pools, raising SOC, POC, and MAOC contents by 2.0–14.2%, 5.7–24.3%, and 1.0–11.9%, respectively, compared to other treatments. (3) In this study, stability of soil aggregates parameters (R_>0.25, MWD and GMD) increased combined with higher levels of bulk SOC and >0.053 mm MAOC, but decreased with higher fractal dimension, indicating a direct causal link between organic carbon accumulation and the betterment of soil structure. Overall, NT-SMR promotes aggregate stability through an optimized particle-size distribution and increased SOC, particularly in the >0.053 mm MAOC fraction. This practice is a sustainable long-term strategy for enhancing SOC sequestration and structural stability in paddy. Full article

(This article belongs to the Special Issue Sustainable Management and Tillage Practice in Agriculture—2nd Edition)

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22 pages, 1168 KB

Open AccessArticle

Hydrochar as a Modulator of Soil Microbial Activity and Soil Biochemical Processes

by Francisco J. Moreno-Racero, Marta Velasco-Molina, Rafael López-Núñez and Heike Knicker

Agronomy 2026, 16(9), 917; https://doi.org/10.3390/agronomy16090917 - 30 Apr 2026

Viewed by 304

Abstract

Hydrochar has emerged as a promising carbonaceous amendment to enhance soil quality, yet its short-term effects on soil carbon (C) and nitrogen (N) dynamics and microbial functioning remain poorly understood. Here, a 77-day greenhouse pot experiment was conducted using a Cambisol cultivated with [...] Read more.

Hydrochar has emerged as a promising carbonaceous amendment to enhance soil quality, yet its short-term effects on soil carbon (C) and nitrogen (N) dynamics and microbial functioning remain poorly understood. Here, a 77-day greenhouse pot experiment was conducted using a Cambisol cultivated with sunflower (Helianthus annuus L.) under two irrigation regimes simulating well-irrigated (WI) and water-deficit (WD) scenarios. Two doses of chicken-manure-derived hydrochar (3.25 and 6.5 t ha⁻¹, corresponding to 2.35 and 4.69 g kg⁻¹ of dry soil, respectively) and mineral fertilizer (MF) treatments providing equivalent N inputs were evaluated. Hydrochar promoted microbial growth and enhanced enzymatic and respiratory activities despite its low apparent C and nutrient input. After 77 days under WI, the addition of 6.5 t ha⁻¹ hydrochar enhanced the activity of phenol oxidase (POA) and acid phosphomonesterase (AcPA). Concomitantly, the availability of soluble C and N increased, whereas total organic C (TOC) and N decreased relative to the initial values. These responses may suggest enhanced mineralization potentially related to early-stage priming processes. The increase in POA relative to β-glucosidase is in line with a functional shift from a predominant degradation of labile compounds towards an increased oxidation of more complex structures. This interpretation is supported by solid-state ¹³C NMR data, revealing a higher degradation index of the soil organic matter. Under WD, the overall effects of hydrochar were attenuated or suppressed, particularly those related to C and N dynamics, emphasizing the interactive influence of moisture and amendment dose. Overall, our results show that hydrochar can modulate short-term soil biochemical processes, partly through enhanced microbial responses. Full article

(This article belongs to the Section Soil and Plant Nutrition)

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16 pages, 2584 KB

Open AccessArticle

The Effect of the Freeze–Thaw Process on Plant Available Water and Water-Stable Aggregates as a Function of Soil Tillage and Soil Chemical Quality

by Mykola Kochiieru, Simona Pranaitienė, Virginijus Feiza and Yuliia Kochiieru

Agronomy 2026, 16(9), 916; https://doi.org/10.3390/agronomy16090916 - 30 Apr 2026

Viewed by 376

Abstract

The goal of this work was to determine the effect of soil freeze–thaw processes on the formation of water-stable aggregates (WSA) and plant available water (PAW) in soils of different textures, depending on the intensity of tillage: conventional tillage (CT), reduced tillage (RT) [...] Read more.

The goal of this work was to determine the effect of soil freeze–thaw processes on the formation of water-stable aggregates (WSA) and plant available water (PAW) in soils of different textures, depending on the intensity of tillage: conventional tillage (CT), reduced tillage (RT) and no-tillage (NT). The WSA value (0.4%) and PAW mean (5.5%) in sandy loam were higher than in loam. The average content of WSA and PAW tended to decrease in the following order: air-dry soil > soil with water content at field capacity > soil near full saturation. These results indicate that WSA in soils that are close to full saturation upon freezing will be less stable after thawing and will decrease the PAW. The content of WSA in NT was 9.4% higher than in RT and 14% higher than in CT. The content of PAW in NT was 5.6% higher than in CT and 13.6% higher than in RT. The effects of various physical and chemical properties on PAW as a function of water level during the freeze–thaw process indicate that WSA content acted as a direct factor for PAW. In a temperate climate zone under dry meteorological conditions, NT would have a promising future for soil stability by maintaining higher WSA and PAW. Full article

(This article belongs to the Special Issue Integrated Evaluation of Greenhouse Gas Emissions in Organic Farming Systems)

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20 pages, 2033 KB

Open AccessArticle

Intra- and Interspecific Competition Between Ile1781Leu ACCase-Resistant and Susceptible Johnsongrass (Sorghum halepense) Populations and Corn or Sunflower

by Aristeidis P. Papapanagiotou, Ioannis Vasilakoglou and Ilias G. Eleftherohorinos

Agronomy 2026, 16(9), 915; https://doi.org/10.3390/agronomy16090915 - 30 Apr 2026

Viewed by 244

Abstract

The evolution of herbicide resistance can increase, decrease, or have no effect on the growth rate, competitive ability, and fitness of field-selected populations. The growth response of an ACCase-resistant (R) johnsongrass [Sorghum halepense (L.) Pers.] population harboring an Ile1781Leu mutation, and a [...] Read more.

The evolution of herbicide resistance can increase, decrease, or have no effect on the growth rate, competitive ability, and fitness of field-selected populations. The growth response of an ACCase-resistant (R) johnsongrass [Sorghum halepense (L.) Pers.] population harboring an Ile1781Leu mutation, and a susceptible (S) population was studied in pot experiments under intraspecific and interspecific competition with corn or sunflower, using a target-neighborhood design. The R population in the intraspecific competition indicated greater fitness-related traits such as height (H), tiller number (TN), aboveground fresh weight (AFW), and rhizome fresh weight (RFW) than the S population. Aggressiveness, competitive ratio, competition intensity index, and relative competition intensity indices confirmed also the superiority of the R population. Similarly, the R population grown in interspecific competition with corn or sunflower produced greater H, TN, and AFW than the S population. In addition, both R and S populations growing in competition with corn produced more H, TN, and AFW than those growing in competition with sunflower. Furthermore, the R population in competition with corn hybrids resulted in a greater reduction in H and AFW in corn plants. These findings strongly support the evidence of fitness advantage in the R population harboring the 1781Leu mutant allele as compared to the S counterpart. Full article

(This article belongs to the Section Weed Science and Weed Management)

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20 pages, 6470 KB

Open AccessArticle

Endogenous Anthocyanins Are Associated with Improved Starch Quality Stability in Black Rice Under Accelerated Aging: Evidence from an OsKala4 Knockout Model

by Wanxin Gong, Lujing Luo, Siyuan Pu, Yi Zhou, Zhijie Liu, Dianxing Wu and Ning Zhang

Agronomy 2026, 16(9), 914; https://doi.org/10.3390/agronomy16090914 - 30 Apr 2026

Viewed by 258

Abstract

Black rice is rich in anthocyanins with potential antioxidant benefits, but their specific role in storage stability remains unclear due to confounding genetic backgrounds in previous studies. In this study, we used CRISPR/Cas9-mediated gene editing to generate OsKala4 knockout lines in the black [...] Read more.

Black rice is rich in anthocyanins with potential antioxidant benefits, but their specific role in storage stability remains unclear due to confounding genetic backgrounds in previous studies. In this study, we used CRISPR/Cas9-mediated gene editing to generate OsKala4 knockout lines in the black rice cultivar Heizhen (HZ), creating an isogenic system to test whether endogenous anthocyanins contribute to storage-related quality stability. Knockout lines showed blocked anthocyanin biosynthesis (0.5–0.6 vs. 155.6 mg/100 g, p < 0.001) and altered grain composition. Under accelerated aging (45 °C, 90% RH, 2 weeks), HZ maintained higher antioxidant capacity (p < 0.05) and exhibited less pronounced starch aging than the representative knockout line KO2. Apparent amylose content increased less in HZ than in KO2 (16.7% vs. 28.1%, p < 0.05). HZ also showed smaller changes in pasting, thermal, and structural properties. XRD and FTIR analyses further suggested better maintenance of starch crystallinity and molecular order in HZ under accelerated aging conditions. These results suggest that endogenous anthocyanins were associated with storage-related quality stability in black rice. However, direct mechanistic evidence and validation under natural storage conditions are still needed. Full article

(This article belongs to the Section Plant-Crop Biology and Biochemistry)

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17 pages, 2713 KB

Open AccessArticle

Energetic Constraints and Carbon Efficiency During Sucrose Storage in Sugarcane Culms

by Frederik C. Botha

Agronomy 2026, 16(9), 913; https://doi.org/10.3390/agronomy16090913 - 30 Apr 2026

Viewed by 247

Abstract

Sugarcane stores sucrose in a living culm for extended periods, yet the respiratory cost of maintaining this storage tissue remains poorly quantified. We quantified growth and maintenance respiration along the culm (internodes 1 to 12) in three genotypes at mid-season (rapid growth) and [...] Read more.

Sugarcane stores sucrose in a living culm for extended periods, yet the respiratory cost of maintaining this storage tissue remains poorly quantified. We quantified growth and maintenance respiration along the culm (internodes 1 to 12) in three genotypes at mid-season (rapid growth) and end-season (maturation) using a composition-based carbon accounting framework derived from measurements of biomass accumulation and composition. Growth respiration was highest in elongating internodes (3 to 6) and declined with maturation, whereas maintenance respiration increased progressively and dominated in mature storage internodes (10 to 12). Consequently, total sink demand remained substantial even after structural growth slowed, indicating that mature internodes continue to require significant metabolic input despite limited biomass production. To evaluate the potential impact of energetic constraints, we simulated reduced mitochondrial energy contribution to assess the sensitivity of respiratory carbon demand to decreased energetic efficiency. These simulations predicted an increase in glucose requirement for respiration across all internodes, with the largest proportional effect in mature tissue where maintenance costs dominated. Despite this predicted increase in respiratory demand, sucrose accumulation was maintained in mature culms, indicating that respiratory carbon loss remains constrained during storage. This suggests that storage tissue operates with relatively high carbon-use efficiency during maintenance-dominated metabolism. We interpret this pattern as consistent with metabolic configurations that reduce ATP demand, potentially involving partial substitution of ATP-dependent reactions by pyrophosphate (PPi)-dependent pathways, although this mechanism was not directly measured. These findings highlight the importance of maintenance respiration and energetic efficiency in determining sink strength and sucrose yield, and they provide a physiological framework for understanding carbon conservation in long-lived storage organs. Full article

(This article belongs to the Section Plant-Crop Biology and Biochemistry)

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24 pages, 992 KB

Open AccessArticle

Field-Based Evaluation of Reactive Oxygen Species Treatments and Fungicide Protections in Potato: Effects on Late Blight, Plant Nutritional Status, Yield, and Tuber Quality

by Karol Skrobacz, Małgorzata Szostek and Maciej Balawejder

Agronomy 2026, 16(9), 912; https://doi.org/10.3390/agronomy16090912 - 30 Apr 2026

Viewed by 269

Abstract

The aim of the study was to determine, under field conditions, the effects of O₃, H₂O₂, and fungicide protection on potato late blight severity, SPAD values, tuber yield, and mineral composition, and additionally to assess whether the [...] Read more.

The aim of the study was to determine, under field conditions, the effects of O₃, H₂O₂, and fungicide protection on potato late blight severity, SPAD values, tuber yield, and mineral composition, and additionally to assess whether the number of ozone applications modifies selected tuber quality traits. Two complementary field experiments were conducted in 2017 and 2018. In the main experiment, control, fungicide protection, ozone fumigation, and foliar H₂O₂ treatments were compared with respect to late blight severity, SPAD response, yield, and macro- and micronutrient contents in tuber peel and flesh. In the supplementary experiment, single, double, and triple ozonation were compared in relation to starch content, vitamin C concentration, and tuber mineral composition. Fungicide treatment most effectively limited late blight symptoms, particularly at later assessment dates, and was associated with the highest tuber yield. SPAD values, yield, and several mineral traits were strongly dependent on the study year, indicating a major contribution of environmental conditions. The response to O₃ and H₂O₂ was selective and less stable than that observed under fungicide protection. In the supplementary experiment, the number of ozone applications did not significantly affect starch content. Vitamin C concentration depended mainly on the study year, whereas tuber mineral composition depended mainly on year and tissue type. The results indicate that, under field conditions, fungicide protection remained the most effective option for limiting late blight and achieving the highest tuber yield, whereas O₃ and H₂O₂ should be regarded as factors capable of modifying selected plant and tuber traits, but not as direct substitutes for standard chemical protection. Full article

(This article belongs to the Special Issue Harnessing Reactive Oxygen Species (ROS) for Crop Performance)

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Agronomy, Volume 16, Issue 9 (May-1 2026) – 97 articles

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