Bionanotechnology is a relatively new term that implies the use of some biological material or organisms in order to prepare nanosystems or nanoparticles. This work presents the preparation and bactericide application of a sustainable nanometric system (silver nanoparticles) using a waste biological support (bovine bone powder). This system was prepared by the method of metallic salt reduction, using NaBH4
as reducing agent and AgNO3
as metallic salt. Two silver contents were analyzed, 1% and 5% weight. The latter was found to be more efficient than the former. Transmission electronic microscopy shows an average size of 10.5 ± 3.3 nm and quasi-sphere morphology. The antimicrobial assay shows that a 5% weight content of silver had a bactericide effect for Escherichia coli
at 46.8 min of exposure. The minimum inhibitory concentration (MIC) of silver nanoparticles supported on bovine bone powder for Escherichia coli
was 7.5 µg/mL. The biocomposite exhibits a specific antibacterial kinetics constant (k) of 0.1128 min−1
and decimal reduction time (DRT) of 20.39 min for Escherichia coli
. Thus, it was concluded that a biocomposite was prepared with a biodegradable, waste, and low-cost support, under mild conditions (room temperature and atmospheric pressure) and using water as solvent.