Surveillance of Microbial and Parasitic Agents in Wildlife: Second Edition

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Wildlife".

Deadline for manuscript submissions: 25 January 2026 | Viewed by 2293

Special Issue Editors


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Guest Editor
Laboratório de Imunologia e Biologia Molecular, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, UFRGS, Porto Alegre 91540-000, RS, Brazil
Interests: virus; zoonosis; phylogeny
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Guest Editor
Laboratório de Microbiologia Molecular, Instituto de Ciências da Saúde, Universidade FEEVALE, Novo Hamburgo 93700-000, RS, Brazil
Interests: metagenomics; virus; zoonosis; wildlife; microbials
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The rise in human-generated noise worldwide has brought increased awareness of its harmful impact on wildlife. Most emerging infectious diseases in humans originate from animals, with human-driven environmental disruptions playing a major role in the appearance of new pathogens. Understanding the fundamental processes behind the emergence of these pathogens is essential for identifying and addressing key human activities that contribute to outbreaks, epidemics, and pandemics. Additionally, maintaining vigilant monitoring of both human and animal microbiomes, as well as genetic parasites, is critical to preventing future spillover events. In the same way, investing in interdisciplinary research that explores the interactions between human, animal, and environmental microbiomes can offer deeper insights for preventing future pandemics. The confirmed origin of SARS-CoV-2 from a wild animal host strongly supports this perspective.

Prof. Dr. Matheus Weber
Dr. Mariana Soares Da Silva
Guest Editors

Manuscript Submission Information

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Keywords

  • microbials
  • zoonosis
  • wildlife
  • pathogens
  • outbreaks

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Related Special Issue

Published Papers (2 papers)

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Research

12 pages, 3054 KiB  
Article
Characterization of Three Novel Papillomavirus Genomes in Vampire Bats (Desmodus rotundus)
by Laura Junqueira de Camargo, Raquel Silva Alves, Raíssa Nunes dos Santos, Letícia Ferreira Baumbach, Juliana do Canto Olegário, Vitória Rabaioli, Matheus de Oliveira Silva, André Alberto Witt, Fernanda Marques Godinho, Richard Steiner Salvato, Matheus Nunes Weber, Mariana Soares da Silva, Cíntia Daudt, Renata da Fontoura Budaszewski and Cláudio Wageck Canal
Animals 2024, 14(24), 3604; https://doi.org/10.3390/ani14243604 - 14 Dec 2024
Cited by 1 | Viewed by 1026
Abstract
Bats are mammals with high biodiversity and wide geographical range. In Brazil, three haematophagous bat species are found. Desmodus rotundus is the most documented due to its role as a primary host of rabies virus in Latin America. Bats are known to harbor [...] Read more.
Bats are mammals with high biodiversity and wide geographical range. In Brazil, three haematophagous bat species are found. Desmodus rotundus is the most documented due to its role as a primary host of rabies virus in Latin America. Bats are known to harbor various emerging viruses causing severe human diseases. Beyond zoonotic viruses, these animals also harbor a diversity of non-zoonotic viruses. Papillomaviruses are circular double-stranded deoxyribonucleic acid (dsDNA) viruses that infect the epithelial and mucosal cells of many vertebrates, occasionally causing malignant lesions. High-throughput sequencing has enabled papillomaviruses discovery in different bat species. Here, 22 D. rotundus samples were collected through the rabies eradication program in Rio Grande do Sul. The DNA extracted from pooled intestines was amplified by the rolling-circle amplification (RCA) method and sequenced using the Illumina® MiSeq platform (San Diego, CA, USA).Analysis revealed three contigs corresponding to the Papillomaviridae family, representing three novel viruses named DrPV-1, DrPV-2, and DrPV-3. Phylogenetic analysis suggests DrPV-1 may constitute a new species within the Dyophipapillomavirus genus, while DrPV-2 and DrPV-3 may represent different types within the same species from a novel genus. This is the first description of a papillomavirus in the D. rotundus species, contributing to the characterization of PVs in the Chiropteran order. Full article
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13 pages, 1503 KiB  
Article
Multiple Typing Approach to Characterize Toxoplasma gondii Strains from Captive and Livestock Species in Northern Italy Suggests the Circulation of Type-II Variants
by Filippo Maria Dini, Martha Ynés Salas-Fajardo, Roberta Taddei, Mattia Ramini, Silvia Vianello, Monica Caffara and Roberta Galuppi
Animals 2024, 14(24), 3597; https://doi.org/10.3390/ani14243597 - 12 Dec 2024
Viewed by 866
Abstract
Toxoplasma gondii is a widespread foodborne parasite that affects both humans and animals worldwide. The genetic characterization of this parasite has become crucial due to its epidemiological and clinical implications. The present study focused on the direct genetic characterization of T. gondii-positive DNA [...] Read more.
Toxoplasma gondii is a widespread foodborne parasite that affects both humans and animals worldwide. The genetic characterization of this parasite has become crucial due to its epidemiological and clinical implications. The present study focused on the direct genetic characterization of T. gondii-positive DNA samples from Northern Italy, using three standardized genotyping methods. Of the 87 PCR-positive DNA samples obtained from livestock, wild, and captive animals, 9 were confirmed, quantified, and subsequently subjected to typing procedures: microsatellite analysis, PCR-RFLP, and partial sequencing of GRA6 and SAG3 genes. Most of the typed samples, derived from clinical cases of toxoplasmosis, specifically related to abortion outbreaks in small ruminants, as well as one case of systemic toxoplasmosis in lemur (Lemur catta). Predominantly, Type II strains were observed, with non-clonal variants identified in a sheep and a lemur. Sequencing of SAG3 revealed specific single nucleotide polymorphism, previously documented in European type-II variants, with phylogenesis suggesting a potential genetic relatedness between T. gondii population in Italy, Spain, and France. By utilizing three distinct genotyping methods, this study provides the first comprehensive microsatellite profiles of T. gondii in Italy, and additional insights into the molecular variability of T. gondii strains circulating in this country from different epidemiological settings. Full article
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