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22 pages, 9306 KB

Open AccessArticle

Dietary Bacillus subtilis PB6 Enhances Reproductive Performance by Modulating Gut Microbiota, Barrier Function, and Inflammation in Clostridium perfringens Type A-Infected Sows

by Mengran Zhang, Aohang Yu, Chihao Wang, Chaojie Chen and Chenchen Wu

Animals 2026, 16(7), 1032; https://doi.org/10.3390/ani16071032 - 27 Mar 2026

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Abstract

Bacillus subtilis is aerobic or facultatively anaerobic. After entering the gastrointestinal tract, its spores germinate and colonize the gut, inhibiting the growth of harmful aerobic bacteria (Escherichia coli, Streptococcus, Staphylococcus aureus). However, it remains unclear whether B. subtilis can [...] Read more.

Bacillus subtilis is aerobic or facultatively anaerobic. After entering the gastrointestinal tract, its spores germinate and colonize the gut, inhibiting the growth of harmful aerobic bacteria (Escherichia coli, Streptococcus, Staphylococcus aureus). However, it remains unclear whether B. subtilis can inhibit Clostridium perfringens type A infection. In this study, B. subtilis PB6 was added to the diets of pregnant sows infected with Clostridium perfringens type A, which significantly improved the reproductive performance and reduced the incidence of bloat in sows and diarrhea in neonatal piglets. The treatment significantly increased the abundance of intestinal probiotics (B. subtilis, Lactobacillus, Limosilactobacillus reuteri, Lactobacillus johnsonii, Muribaculaceae, Lactobacillus amylovorus, and Lactobacillus reuteri) in sows and decreased the relative abundance of Clostridium perfringens type A after feeding B. subtilis administration. These probiotics can repair the intestinal tissue and improve intestinal histomorphology, and enhance the expression of MUC2 and sIgA in sows, thereby further strengthening the mucosal immune function. B. subtilis can also reduce the levels of inflammatory factors (CRP, IL-1β, and IFN-γ) and attenuate the inflammatory response in sows and neonatal piglets. Taken together, our results suggest that dietary supplementation with B. subtilis PB6 could reduce bloat in sows and diarrhea in piglets while improving intestinal barrier function and microbial balance in sows. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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16 pages, 713 KB

Open AccessArticle

Adding a Yeast Blend to the Diet of Holstein Females Minimizes the Negative Impacts of Ingesting Feed Naturally Contaminated with Aflatoxin B1

by Mario Augusto Torteli, Andrei Lucas Rebelatto Brunetto, Emeline P. Mello, Guilherme Luiz Deolindo, Luisa Nora, Tainara Letícia dos Santos, Luiz Eduardo Lobo e Silva, Roger Wagner and Aleksandro Schafer da Silva

Animals 2026, 16(2), 219; https://doi.org/10.3390/ani16020219 - 12 Jan 2026

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Abstract

Although a yeast-based additive was initially employed as a performance enhancer, subsequent analysis revealed high aflatoxin B1 levels in the corn silage. Therefore, the objective of this study is to determine if the use of a yeast blend in the diet of Holstein [...] Read more.

Although a yeast-based additive was initially employed as a performance enhancer, subsequent analysis revealed high aflatoxin B1 levels in the corn silage. Therefore, the objective of this study is to determine if the use of a yeast blend in the diet of Holstein calves that consumed feed naturally contaminated with high levels of aflatoxin can minimize the negative impacts of mycotoxins on animal health, contributing to improved performance. For this, we used 24 Holstein calves (6 months old) divided into two groups: Control (n = 12; no additive) and Treatment (n = 12; 5 g additive/animal/day). During the 100-day experiment, animals were weighed, feed intake was measured, blood samples were collected to assess health, and ruminal fluid was analyzed for ruminal fermentation. We observed greater weight gain and better feed efficiency in cattle that consumed the yeast-based additive compared to the control group. Yeast ingestion increased the concentration of propionic acid in the experimental environment, as well as increasing the protozoan count. Higher lymphocyte counts combined with higher levels of immunoglobulin G in the blood of females that consumed the additive were observed. Lower activity of enzymes that are biomarkers of liver damage, as well as markers of oxidative stress, was observed when animals consumed the yeast blend compared to the control group. Lower levels of ceruloplasmin (positive acute phase protein) and higher levels of transferrin (negative acute phase protein) are indicative of an anti-inflammatory response to the additive. The results preliminarily suggest that the consumption of the yeast blend is a nutritional tool capable of acting as a performance enhancer, even under challenging conditions, such as diets contaminated with aflatoxin at levels exceeding international limits. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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19 pages, 321 KB

Open AccessArticle

Dietary Amylose/Amylopectin Ratio Modulated Growth Performance, Intestinal Health, and Lipid Metabolism in Goslings Fed Low-Protein Diets

by Xiyuan Xing, Xucheng Zheng, Xuan Li, Zhi Yang, Haiming Yang and Zhiyue Wang

Animals 2026, 16(2), 189; https://doi.org/10.3390/ani16020189 - 8 Jan 2026

Cited by 2 | Viewed by 1192

Abstract

This study investigated the effects of amylose/amylopectin (AM/AP) ratios in low-protein (LP) diets on the growth performance, fat deposition, and nutrient utilization in goslings. A total of 288 healthy, 35-day-old male Jiangnan White Geese were randomly divided into four treatment groups: one group [...] Read more.

This study investigated the effects of amylose/amylopectin (AM/AP) ratios in low-protein (LP) diets on the growth performance, fat deposition, and nutrient utilization in goslings. A total of 288 healthy, 35-day-old male Jiangnan White Geese were randomly divided into four treatment groups: one group fed a normal protein diet (16%) with an AM/AP ratio of 0.34 (NPR_0.34), and three groups fed low protein diets (14%) with different AM/AP ratios (LPR_0.26, LPR_0.34, LPR_0.44). Each group consisted of six replicates, with 12 geese per replicate, and they were fed for 28 days. The results showed that the body weight at 63 days and average daily gain (ADG) of the LPR_0.44 group geese were significantly higher than those of the other groups (p < 0.01), while the feed-to-gain ratio (F/G) was lower (p < 0.05). The abdominal and mesenteric fat contents were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05), whereas the breast and leg muscle yields were higher (p < 0.05). The breast muscle redness (a*) of the LPR_0.34 and LPR_0.44 groups was higher than in the NPR_0.34 group at 45 min (p < 0.05). The levels of C6:0, C8:0, C11:0, C12:0, and C13:0 in breast muscle saturated fatty acids (SFAs) of the LPR_0.44 group were higher, while that of C18:0 was lower compared with the LPR_0.26 group (p < 0.05). The serum total cholesterol (TC) and triglycerides (TGs) in the LPR_0.44 group were lower than in the LPR_0.26 group (p < 0.05). Hepatic lipase (HL) activity was significantly lower in the LPR_0.44 group (p < 0.01). Regarding hepatic fatty acids, the levels of butyric acid (C4:0), lauric acid (C12:0), and nervonic acid (C24:1) were lower in the LPR_0.44 group than in the LPR_0.26 group (p < 0.05). No significant differences were observed in intestinal morphology, digestive enzyme activities, or nutrient utilization among the groups. (p > 0.05). In conclusion, adjusting the AM/AP ratio to 0.44 in a low-protein diet improved growth performance, regulated lipid metabolism, and maintained intestinal function in goslings. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

16 pages, 3099 KB

Open AccessArticle

Hermetia illucens Larvae Meal Enhances Colonic Antimicrobial Peptide Expression by Promoting Histone Acetylation in Weaned Piglets Challenged with ETEC in Pig Housing

by Qingsong Tang, Guixing Wu, Wentuo Xu, Jingxi Liu, Huiliang Liu, Bin Zhong, Qiwen Wu, Xuefeng Yang, Li Wang, Zongyong Jiang and Hongbo Yi

Animals 2026, 16(1), 118; https://doi.org/10.3390/ani16010118 - 31 Dec 2025

Cited by 1 | Viewed by 581

Abstract

The objective of this study was to investigate the effects of replacing fishmeal with H. illucens larval meal on the colonic immune homeostasis in weaned piglets in enterotoxigenic Escherichia coli (ETEC)-challenged pig housing. Seventy-two weaned piglets, aged 28 days, were randomly divided into [...] Read more.

The objective of this study was to investigate the effects of replacing fishmeal with H. illucens larval meal on the colonic immune homeostasis in weaned piglets in enterotoxigenic Escherichia coli (ETEC)-challenged pig housing. Seventy-two weaned piglets, aged 28 days, were randomly divided into three groups for dietary treatment: the basal diet (negative control, NC), the positive control diet (PC) supplemented with 1445 mg zinc/kg zinc oxide in the basal diet, and the H. illucens larval meal complete replacement of fishmeal in the basal diet (HILM), for 28 days in ETEC-challenged pig housing. The results showed that the relative transcript abundances of ZO-1, pBD2, PR39, and PG1–5 were increased (p < 0.05) in pigs fed the HILM diet compared with those fed the NC diet. In addition, the HILM diet reduced (p < 0.05) the serum contents of IL-8 and increased (p < 0.05) the serum contents of IL-10 and IgG compared with the NC diet. In terms of the molecular mechanisms by which immune homeostasis is improved, the p-NF-κB/ NF-κB ratio and TLR2 protein expression in the colon were decreased (p < 0.05) in pigs fed the HILM diet compared with those fed the NC diet. Compared with the NC diet, the HILM diet reduced (p < 0.05) the protein expression of HDAC3 and HDAC7 in the colon of pigs. The SIRT1, acH3K9, and pH3S10 protein expressions in the colon were the greatest (p < 0.05) in pigs fed the HILM diet compared with the NC diet. HILM diets improved the colonic immune homeostasis in weaned piglets by enhancing the antimicrobial peptide expression, thereby mitigating ETEC challenges in pig housing. Mechanistically, HILM diets promote antimicrobial peptide expression through increased histone acetylation (acH3K9 and pH3S10). Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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19 pages, 277 KB

Open AccessArticle

Determination of Standardized Ileal Amino Acid Digestibilities in Different Soybean Meals for Yellow-Feathered Chickens and Development of Prediction Models

by Qianwen Yuan, Wenpeng Chen, Jiali Long, Liyang Zhang, Shengchen Wang, Tingting Li, Yun Hu, Xiaoyan Cui and Xugang Luo

Animals 2026, 16(1), 89; https://doi.org/10.3390/ani16010089 - 28 Dec 2025

Viewed by 859

Abstract

Currently, there is no report on prediction models of standardized ileal amino acid digestibilities (SIAADs) in soybean meals (SBMs) for medium-growing yellow-feathered chickens. This study firstly analyzed the chemical compositions of 10 SBMs, then determined their SIAADs in chickens, and finally established and [...] Read more.

Currently, there is no report on prediction models of standardized ileal amino acid digestibilities (SIAADs) in soybean meals (SBMs) for medium-growing yellow-feathered chickens. This study firstly analyzed the chemical compositions of 10 SBMs, then determined their SIAADs in chickens, and finally established and verified prediction models for SBM SIAADs based on their chemical compositions and amino acid (AA) profiles. A total of 276 55 d-old Tianluma roosters were selected and randomly divided by body weight into 11 treatment groups. On d 63, chickens were fed either a nitrogen-free diet (NFD) or one of 10 SBM diets for 5 d. On d 67, ileal chyme samples were collected to determine SIAADs. Data from nine SBM samples and stepwise regressions were employed to build prediction models, while one SBM sample was randomly selected to validate model accuracy. Different SBM sources affected (p ≤ 0.007) SIAADs in medium-growing yellow-feathered chickens. The standardized ileal digestibility (SID) of glutamic acid (Glu) was the highest (93.9%), whereas that of cysteine (Cys) was the lowest (81.7%). Fifteen prediction models (R² = 0.567–0.993, p < 0.03) for the SIDs of methionine (Met), isoleucine (Ile), leucine (Leu), phenylalanine (Phe), lysine (Lys), histidine (His), arginine (Arg), aspartic acid (Asp), serine (Ser), Glu, glycine (Gly), alanine (Ala), Cys, tyrosine (Tyr), and proline (Pro) in SBMs for medium-growing yellow-feathered chickens were effectively established based on chemical compositions and AA profiles. Among them, the prediction model for the SID of Cys showed the best fit (R² = 0.993, p = 0.002), while the model for the SID of Ala had the lowest fit (R² = 0.567, p = 0.019). Except for His and Pro, which exhibited poor predictive accuracy, all other models showed good accuracy. These prediction models thus provide a valuable reference for rapidly estimating the SIDs of key AAs in SBMs for medium-growing yellow-feathered chickens. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

14 pages, 269 KB

Open AccessArticle

Impact of Dietary Protein Levels and Gender on Carcass Characteristics and Meat Quality in Slow-Growing Ducks

by Yong Jiang, Yijia Lu, Zhong Zhuang, Lei Wu, Yongpeng Li, Hao Bai, Yulin Bi, Zhixiu Wang, Shihao Chen and Guobin Chang

Animals 2026, 16(1), 79; https://doi.org/10.3390/ani16010079 - 26 Dec 2025

Viewed by 716

Abstract

The aim of the present study was to investigate the effects of different dietary protein levels on the carcass traits, meat quality characteristics, and nutrient composition of slow-growing ducks. At 22 days of age, the ducks were randomly divided into two groups and [...] Read more.

The aim of the present study was to investigate the effects of different dietary protein levels on the carcass traits, meat quality characteristics, and nutrient composition of slow-growing ducks. At 22 days of age, the ducks were randomly divided into two groups and fed with low- or high-protein diets for 41 days, from 22 to 63 days of age. Each group consisted of six replicates, with each replicate containing 500 ducklings per pen (10 m × 10 m). The results showed that dietary protein had no significant effects on carcass traits, meat quality, amino acid profiles in breast muscle and thigh muscle, and fatty acid contents in breast muscle. However, it increased the contents of C14:0, C16:0, C16:1, C18:1, C20:4, SFA, MUFA, and ω-6 fatty acids (p < 0.05), and reduced the contents of C22:6, ω-3 fatty acids, and ω-3/ω-6 ratio in thigh muscle (p < 0.05). Female ducks fed with a low-protein diet had the contents of aspartic acid, threonine, serine, glutamic acid, glycine, tyrosine, and arginine in the breast muscle, along with a higher pH24 value (p < 0.05). Thigh muscle accumulated more isoleucine and histidine contents, and lower lysine and arginine in female ducks fed with a low-protein diet. Male ducks fed with a low-protein diet had higher contents of alanine, valine, methionine, isoleucine, leucine, and lysine in the breast muscle (p < 0.05). Furthermore, male ducks exhibited higher contents of C16:0, C18:1, C18:2, C18:3, saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), ω-3, and ω-6 in breast muscle (p < 0.05). Male ducks fed with low-protein diets had higher C16:0 content in breast muscle, and female ducks fed with a low-protein diet had lower C16:1 and C17:0 contents (p < 0.05). In conclusion, this study suggests that dietary protein modulation can differentially regulate amino acid and fatty acid deposition in slow-growing ducks through gender-specific metabolic pathways and exert distinct effects on fatty acid metabolism. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

13 pages, 1846 KB

Open AccessArticle

Effects of Dietary Potassium Diformate Supplementation on Growth Performance, Nutrient Digestibility, Gastrointestinal pH, Jejunal Morphology, Digestive Enzyme Activity, and Antioxidant Status in Weaned Piglets

by Lina Zhang, Yong Cheng, Li Lu, Songtao Zhong, Tiande Zou, Mingren Qu and Jun Chen

Animals 2025, 15(19), 2916; https://doi.org/10.3390/ani15192916 - 7 Oct 2025

Cited by 3 | Viewed by 1053

Abstract

This study evaluated the effects of dietary potassium diformate supplementation on growth performance, nutrient digestibility, gastrointestinal pH, jejunal morphology, digestive enzyme activity, and antioxidant status of weaned piglets in a 28-day trial. Twenty-four weaned piglets were selected and, after a 4-day adaptation period, [...] Read more.

This study evaluated the effects of dietary potassium diformate supplementation on growth performance, nutrient digestibility, gastrointestinal pH, jejunal morphology, digestive enzyme activity, and antioxidant status of weaned piglets in a 28-day trial. Twenty-four weaned piglets were selected and, after a 4-day adaptation period, randomly assigned to 4 treatment groups (n = 6). The dietary treatments included a control diet (basal diet) and 3 diets supplemented with 0.6%, 1.2%, or 1.8% potassium diformate in the basal diet. The results indicated that the feed conversion ratio (FCR) of piglets was reduced by all three potassium diformate supplementation levels compared to the control group (p < 0.05). Additionally, the FCR was decreased in piglets fed the 1.8% potassium diformate-supplemented diet compared to those fed the 1.2% potassium diformate-supplemented diet (p < 0.05). Piglets fed the three potassium diformate-supplemented diets exhibited higher apparent total tract digestibility (ATTD) of dry matter and crude protein than the control group (p < 0.05). The 1.8% potassium diformate groups also showed increased ATTD of calcium and phosphorus compared to the control group (p < 0.05). Supplementation with 1.2% or 1.8% potassium diformate reduced the digesta pH in the proximal stomach, distal stomach, and duodenum, while increased jejunal villus height (VH), VH/crypt depth (VH/CD) ratio, and catalase and total superoxide dismutase activities in the jejunal mucosa compared to the control group (p < 0.05). The 1.2% potassium diformate group showed higher α-amylase activity than the control group (p < 0.05). Correlation analysis revealed that FCR negatively correlated with ATTD of dry matter, crude protein, calcium, phosphorus, and jejunal VH, while positively correlating with digesta pH in the proximal stomach (p < 0.05). The ATTD of dry matter negatively correlated with digesta pH in the proximal stomach, distal stomach, and duodenum, and positively correlated with jejunal VH/CD ratio and catalase activity (p < 0.05). The ATTD of crude protein negatively correlated with digesta pH in the proximal stomach, distal stomach, and duodenum (p < 0.05). Collectively, dietary supplementation with 1.8% potassium diformate reduced FCR of weaned piglets, which was associated with enhanced nutrient digestibility, reduced pH in the anterior gastrointestinal tract, and improved jejunal morphology. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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13 pages, 268 KB

Open AccessArticle

Combination of Anti-Mycotoxin Additive in Diet Contaminated with Multiple Mycotoxins (Aflatoxin, Fumonisin, Zearalenone and Deoxynivalenol): Effects on Performance and Health of Lambs

by Suelyn de Oliveira Marques, Guilherme Luiz Deolindo, Andrei Lucas Rebelatto Brunetto, Ana Lara Amaral da Veiga, Renato Santos de Jesus, Eduardo Micotti Da Gloria, Gilnei Bruno da Silva, Margarete Dulce Bagatini and Aleksandro Schafer Da Silva

Animals 2025, 15(19), 2835; https://doi.org/10.3390/ani15192835 - 28 Sep 2025

Viewed by 1102

Abstract

During the grain and cereal production process, whether during harvesting, processing, or storage, errors can occur, compromising product quality and potentially leading to contamination by fungi, which produce toxic substances known as mycotoxins. When fed to animals, these contaminated grains and cereals can [...] Read more.

During the grain and cereal production process, whether during harvesting, processing, or storage, errors can occur, compromising product quality and potentially leading to contamination by fungi, which produce toxic substances known as mycotoxins. When fed to animals, these contaminated grains and cereals can cause several negative effects on animal health, impacting their production performance, including immunosuppression, hepatotoxicity, nephrotoxicity, and reproductive problems. To minimize the problems caused by mycotoxins, anti-mycotoxin additives, also known as adsorbents, are used. These are inert materials that bind to mycotoxins and are excreted in feces, preventing their action within the animal’s body. Therefore, the objective of this study was to evaluate the effectiveness of an anti-mycotoxin product based on bentonite, activated charcoal, milk thistle extract, and yeast cell wall in the diet of mycotoxin-contaminated lambs on animal health and performance. Thirty lambs were divided into three homogeneous groups: control (without mycotoxins or additives), mycotoxin (with mycotoxins), and anti-mycotoxin (mycotoxins and additive). The mycotoxins used for feed contamination were aflatoxin (AFLA) (200 ppb), fumonisin (FUMO) (15 ppm), zearalenone (ZEA) (500 ppb), and deoxynivalenol (DON) (1.5 ppm). The anti-mycotoxin additive was used at a dose of 1 kg/ton of concentrate. Parameters of zootechnical performance, hematological profile, serum biochemistry, and oxidative status were evaluated. The group that ingested the contaminated concentrate with mycotoxin had a lower average daily weight gain (ADG) when compared to the control and anti-mycotoxin groups. Ingestion of a mycotoxin-contaminated diet increased the activity of aspartate aminotransferase and gamma-glutamyltransferase, which are indicators of liver damage. However, when the anti-mycotoxin additive was used, the increase in these enzymes was modest and lower than in the mycotoxin group. Ingestion of a mycotoxin-containing concentrate increased levels of oxidative stress biomarkers such as reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), myeloperoxidase (MPO), and reduced glutathione (GST), demonstrating that the mycotoxin challenge was effective in causing oxidative stress. However, when the diet was contaminated with mycotoxins and supplemented with the anti-mycotoxin additive, the levels of ROS and TBARS were similar to those of the negative control group. We concluded that adding the anti-mycotoxin product to the lambs’ diets prevented or minimized the problems caused by mycotoxin consumption, allowing these lambs to have ADG, and feed efficiency similar to the control group. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

18 pages, 2941 KB

Open AccessArticle

Effects of Different Forage Sources on Growth Performance, Blood Biochemistry, Hormone Concentrations, and Intestinal Microbiota in Alpacas

by Zhihui Chen, Yang Zhao, Liangmei Xu, Teng Teng and Deying Ma

Animals 2025, 15(17), 2625; https://doi.org/10.3390/ani15172625 - 8 Sep 2025

Cited by 1 | Viewed by 1405

Abstract

Alpaca farming has received increasing attention in China in recent years. However, Australian alfalfa remains the main choice of forage for alpacas. China has abundant forage resources, including oat grass, Leymus chinensis, and corn straw, which are widely distributed across the country. [...] Read more.

Alpaca farming has received increasing attention in China in recent years. However, Australian alfalfa remains the main choice of forage for alpacas. China has abundant forage resources, including oat grass, Leymus chinensis, and corn straw, which are widely distributed across the country. This study aims to explore the feasibility of replacing Australian alfalfa entirely with these resources, thereby providing insights into the selection of forage species for alpacas. Test results showed that oat grass, when used as a fiber source for alpacas, has a significant positive effect on their growth performance, energy metabolism and intestinal microbiota. These results suggest that oat grass is a viable forage option for alpacas and is as nutritionally adequate as alfalfa. In contrast, neither corn straw nor Leymus chinensis demonstrated superior nutritional value compared to alfalfa. Therefore, we conclude that oat grass has substantial potential to serve as an alfalfa substitute in alpaca forage. Conversely, Leymus chinensis and corn straw are not suitable candidates for replacing alfalfa. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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14 pages, 1676 KB

Open AccessArticle

Dietary Glycyl-Glutamine Supplementation Improves Growth, Immunity, Antioxidant Capacity, and Apparent Digestibility of Weaned Piglets

by Xi Jiang, Dong Li, Mengli Chen, Jianzhong Li, Xihong Zhou, Xia Xiong and Yulong Yin

Animals 2025, 15(17), 2573; https://doi.org/10.3390/ani15172573 - 2 Sep 2025

Cited by 2 | Viewed by 1569

Abstract

Glutamine (Gln) supplementation during the weaning period can alleviate stress in piglets. However, free Gln has poor stability and low absorption in the small intestine. Glycyl-glutamine (Gly-Gln), a stable dipeptide form of Gln, has been evaluated as a potential alternative in pig nutrition. [...] Read more.

Glutamine (Gln) supplementation during the weaning period can alleviate stress in piglets. However, free Gln has poor stability and low absorption in the small intestine. Glycyl-glutamine (Gly-Gln), a stable dipeptide form of Gln, has been evaluated as a potential alternative in pig nutrition. This study investigated the effects of Gly-Gln at 0, 0.125%, 0.25%, 0.375%, and 0.50%, as well as a Gly + Gln positive control, on growth performance, intestinal morphology, immunity, antioxidant status, and nutrient apparent digestibility in weaned piglets. The results showed that dietary supplementation with 0.25%, 0.375%, or 0.50% Gly-Gln significantly increased average daily gain, average daily feed intake, and final weight (p < 0.05). Linear and quadratic effects (p < 0.05) were observed for growth performance indicators, suggesting that moderate supplementation levels yielded optimal benefits. Dietary Gly-Gln supplementation with 0.25%, 0.375%, or 0.50% Gly-Gln significantly increased serum immunoglobulin (IgG, IgA, and IgM), insulin, insulin growth factor 1, growth hormone, and T4 and T3 contents, and decreased IFN-γ and IL-1β contents (p < 0.05). Diets supplemented with 0.25, 0.375, or 0.50% Gly-Gln increased total antioxidant capacity and superoxide dismutase content in serum and liver, and decreased MDA content (p < 0.05). Compared with the negative control group, dietary supplementation of 0.25%, 0.375%, or 0.50% Gly-Gln significantly increased the mRNA expression of ZO-1, Occludin, and Claudin-1 in the jejunum (p < 0.05). Furthermore, crude protein digestibility was significantly improved in piglets receiving 0.375% and 0.5% Gly-Gln (p < 0.05), with a significant linear relationship between Gly-Gln level and digestibility. In conclusion, 0.25% is the minimum effective dose of Gly-Gln for improving weaning outcomes. Gly-Gln is more effective than equivalent doses of free glycine and glutamine in enhancing growth performance, gut barrier integrity, and nutrient utilization in weaned piglets. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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18 pages, 2173 KB

Open AccessArticle

Effects of Dietary Protein Levels on the Growth, Physiological, and Biochemical Indices of Juvenile Yellow River Carp (Cyprinus carpio haematopterus)

by Xiaona Jiang, Feihu Qu, Yanlong Ge, Chitao Li, Xiaodan Shi, Xuesong Hu, Lei Cheng, Xinyu Zhao and Zhiying Jia

Animals 2025, 15(12), 1800; https://doi.org/10.3390/ani15121800 - 18 Jun 2025

Cited by 5 | Viewed by 2160

Abstract

An appropriate protein content in the diet can effectively increase the growth rate, muscle quality, and environmental stress resistance of fish. In this study, juvenile Yellow River carp (51.56 ± 0.17 g) were fed isofat diets with different protein concentrations (22%, 25%, 28%, [...] Read more.

An appropriate protein content in the diet can effectively increase the growth rate, muscle quality, and environmental stress resistance of fish. In this study, juvenile Yellow River carp (51.56 ± 0.17 g) were fed isofat diets with different protein concentrations (22%, 25%, 28%, 31%, 34%, and 37%). The results showed that, compared with other protein content groups, when the protein content was 34%, the WGR, SGR, and FCR were significantly higher, while the FCR was significantly lower (p < 0.05). Among them, the SGR and FCR were positively correlated with the dietary protein content, with the regression equations being y₁ = −32.208x² + 21.897x − 1.4001 (R² = 0.8622) and y₂ = 97.027x² − 68.428x + 13.269 (R² = 0.9663), respectively. When x was 33.99% and 35.26%, the SGR and FCR had extreme values. The contents of CP, Lys, EAA, and EAA/TAA were significantly greater in the 34% protein group, reflecting muscle quality (p < 0.05). In addition, the activities of α-AMS, LPS, TPS, SOD, CAT, and GSH-Px were significantly greater in the 34% protein group (p < 0.05). Similarly, the relative expression levels of GH, IGF-I, TOR, 4EBP2, Rhag, Rhbg, and Rhcg1 were significantly greater in the 34% protein group compared to the other protein groups (p < 0.05). The above results indicated that when the protein content in the diet was 34%, it significantly improved the growth and stress resistance of juvenile Yellow River carp. Based on the nonlinear regression equations for the SGR and FCR, the optimal dietary protein content of juvenile Yellow River carp (51.56 ± 0.17 g) was determined to be 33.99–35.26%. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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26 pages, 1181 KB

Open AccessArticle

Replacing Soybean Meal with Hemp Leaves in a Dairy Cow Diet: Plasma Antioxidative Capacity, Inflammatory Parameters and Milk Constituents

by Jessica Schwerdtfeger, Solvig Görs, Dirk Dannenberger and Björn Kuhla

Animals 2025, 15(10), 1414; https://doi.org/10.3390/ani15101414 - 14 May 2025

Cited by 2 | Viewed by 1783

Abstract

Hemp is rich in bioactive compounds known to exert antioxidant and anti-inflammatory properties. We hypothesised that feeding industrial hemp leaves would increase the antioxidative capacity, reduce pro-inflammatory markers, and modulate the milk amino and fatty acid profiles of dairy cows. Twelve Holstein dairy [...] Read more.

Hemp is rich in bioactive compounds known to exert antioxidant and anti-inflammatory properties. We hypothesised that feeding industrial hemp leaves would increase the antioxidative capacity, reduce pro-inflammatory markers, and modulate the milk amino and fatty acid profiles of dairy cows. Twelve Holstein dairy cows were randomly assigned to a total mixed ration (TMR) containing 7.4% industrial hemp leaves (HEMP) of the Santhica 27 variety or a TMR-based diet containing 3.5% soybean meal (CON). Diets were fed in a cross-over design, each for 2 weeks divided by a 2-week washout period. HEMP cows revealed higher plasma and milk cannabinoid concentrations, plasma antioxidative capacity and anserine concentration, but lower plasma π-methylhistidine, α-aminoadipic acid and isoleucine concentrations. Lipid peroxidation and tumour necrosis factor-alpha concentrations were not different between groups. The leukocyte RELA mRNA expression was higher, whereas the TNFA mRNA abundance tended to be lower in the HEMP group. In milk, n-3 polyunsaturated fatty acid (PUFA) concentrations were higher, and the n-6/n-3 PUFA ratio was lower in HEMP than in CON cows. The results provide evidence that the supplementation with Santhica 27 hemp leaves increases the antioxidative capacity of dairy cows and results in a transfer of cannabinoids into milk. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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12 pages, 258 KB

Open AccessFeature PaperArticle

Dietary Schizochytrium sp. Meal Enhances the Fatty Acid Profile in Pirarucu (Arapaima gigas) Fillets with No Effect on Growth Performance and Health Status

by André D. Nobre, Renata V. Mendonça, Ana Beatriz de S. Farias, Fernando Y. Yamamoto and Ligia U. Gonçalves

Animals 2025, 15(5), 712; https://doi.org/10.3390/ani15050712 - 2 Mar 2025

Cited by 4 | Viewed by 2939

Abstract

This study aimed to assess the impact of dietary supplementation with Schizochytrium sp. meal, a source of docosahexaenoic acid (DHA), on the growth, hemato-biochemical parameters, and fatty acid composition of juvenile pirarucu (Arapaima gigas). Two diets were used: a test diet [...] Read more.

This study aimed to assess the impact of dietary supplementation with Schizochytrium sp. meal, a source of docosahexaenoic acid (DHA), on the growth, hemato-biochemical parameters, and fatty acid composition of juvenile pirarucu (Arapaima gigas). Two diets were used: a test diet (5%SZ) and a control diet (0 SZ). Juvenile pirarucu (561.3 ± 13.4 g; 47.5 ± 7.1 cm) were kept in recirculating aquaculture tanks and fed three times daily for 12 weeks. No significant differences in growth performance or adverse effects on hemato-biochemical parameters were found, except for hemoglobin and total protein. The fatty acid profile of the fillets from the fish fed the 5 SZ diet mirrored that of the Schizochytrium sp. meal, with increased levels of palmitic acid, DHA, and eicosapentaenoic acid (EPA), and decreased levels of linoleic and linolenic acids. The inclusion of Schizochytrium sp. meal reduced monounsaturated fatty acids from 39.6% to 32.0% and increased highly unsaturated fatty acids from 3.05% to 11.9% of total lipids. The DHA content was 9.45 times higher in the fillets of the fish fed 5 SZ, improving the n-3/n-6 ratio from 0.26 to 1.19. In conclusion, adding 5% Schizochytrium sp. meal enhances the nutritional quality of pirarucu fillets by increasing DHA and the n-3/n-6 ratio. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

14 pages, 4230 KB

Open AccessArticle

Brown Algae Polysaccharides Alleviate Diquat-Induced Oxidative Stress in Piglets and IPEC-J2 Cells via Nrf2/ARE Signaling Pathway

by Chunjie Hou, Zirou Yu, Chenyu Shi, Ya Huang and Hu Liu

Animals 2025, 15(4), 559; https://doi.org/10.3390/ani15040559 - 14 Feb 2025

Cited by 2 | Viewed by 1345

Abstract

This study investigated the effect of Brown algae polysaccharides (BAPs) on diquat-induced oxidative stress in piglets and IPEC-J2 cells through Nrf2/ARE signaling pathway. In the in vivo model, 24 male piglets of the Duroc × Landrace × Large White breed were selected and [...] Read more.

This study investigated the effect of Brown algae polysaccharides (BAPs) on diquat-induced oxidative stress in piglets and IPEC-J2 cells through Nrf2/ARE signaling pathway. In the in vivo model, 24 male piglets of the Duroc × Landrace × Large White breed were selected and divided into 4 groups (n = 6), including the CON group (basal diet), DIQ group (10 mg/kg Diquat), BAP group (1000 mg/kg BAP), and BAP+DIQ group (1000 mg/kg BAP + 10 mg/kg Diquat). Compared with the DIQ group, BAP improved growth performance and the BAP+DIQ group reduced the levels of IL-1β, IL-6, TNF-α, and DAO in plasma, increased VH and VCR, improved jejunal tissue morphology, decreased MDA levels, and increased T-AOC (p < 0.05). Additionally, the BAP+DIQ group elevated mRNA levels of ZO-1, and enhanced the protein levels of Occludin, Claudin1, CAT, SOD1, and HO-1 (p < 0.05). In the in vitro model, the BAP+DIQ group decreased MDA levels, increased T-AOC, elevated mRNA levels of ZO-1, CAT and SOD2, as well as protein levels of Claudin1, SOD1, HO-1, and total Nrf2 compared with the DIQ group (p < 0.05). Furthermore, BAP increased nuclear Nrf2 protein levels, and promoted the translocation of Nrf2 from the cytoplasm to the nucleus compared with the DIQ group (p < 0.05). In conclusion, BAPs are crucial for enhancing piglets’ antioxidant capacity via Nrf2 pathway activation. These findings highlight BAP’s potential as a natural feed additive to mitigate oxidative stress and improve overall health in piglets. Further research is warranted to explore BAPs as a dietary supplement to support gut health and reduce oxidative stress. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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14 pages, 276 KB

Open AccessArticle

Exploring the Potential Effects of Soybean By-Product (Hulls) and Enzyme (Beta-Mannanase) on Laying Hens During Peak Production

by Muhammad Shuaib, Abdul Hafeez, Deependra Paneru, Woo Kyun Kim, Muhammad Tahir, Anthony Pokoo-Aikins, Obaid Ullah and Abubakar Sufyan

Animals 2025, 15(1), 98; https://doi.org/10.3390/ani15010098 - 4 Jan 2025

Cited by 2 | Viewed by 3116

Abstract

This study determined the interaction between soybean hulls (SHs) and enzymes (β-mannanase) to improve the sustainability and efficacy of feeding programs for laying hens during peak production while ensuring the best health and efficiency. In a completely randomized design (CRD), 200 golden-brown hens [...] Read more.

This study determined the interaction between soybean hulls (SHs) and enzymes (β-mannanase) to improve the sustainability and efficacy of feeding programs for laying hens during peak production while ensuring the best health and efficiency. In a completely randomized design (CRD), 200 golden-brown hens were fed for four weeks (33 to 36 weeks) and randomly distributed into four groups, each containing four replicates of ten birds, with one group receiving a control diet (P0) and the others receiving diets that contained four combinations of SHs and enzymes (ENZs). e.g., 3% SHs and 0.02 g/kg ENZs (P1), 3% SHs and 0.03 g/kg ENZs (P2), 9% SHs and 0.02 g/kg ENZs (P3), and 9% SHs and 0.03 g/kg ENZs (P4). Although most egg quality measures remained similar, the P2 group showed enhanced (p = 0.630) egg weight, albumen weight, and height. Moreover, the P2 group improved gut (p < 0.05) shape by increasing villus width, height, crypt depth, and surface area throughout intestinal sections, while the P4 group markedly improved total cholesterol and LDL (p = 0.022) levels. The P1, P2, and P4 groups exhibited a significant enhancement in dry matter (p = 0.022) and crude fiber (p = 0.046) digestibility, while the P2 group demonstrated superior crude protein digestibility (p = 0.032), and the P1 and P2 groups showed increased crude fat digestibility compared to the other groups. In conclusion, adding 3% of SHs and 30 mg/kg of ENZs (β-mannanase) to the feed may help laying hens, enhance gut health and some egg quality indices, and decrease blood cholesterol and LDL levels without compromising nutrient digestibility. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

12 pages, 259 KB

Open AccessArticle

Effects of Dietary Schizochytrium Algae as ω-3 PUFA Source on the Egg-Laying Quail Performance, Serum Indexes, and Egg Yolk Fatty Acids Contents

by Yifan Chang, Yue Xuan, Ruinan Zhang, Xuemei Ding, Qiufeng Zeng, Jianping Wang, Shiping Bai, Shanshan Li, Yan Liu, Yuchuan Chen and Keying Zhang

Animals 2025, 15(1), 21; https://doi.org/10.3390/ani15010021 - 25 Dec 2024

Cited by 7 | Viewed by 3233

Abstract

The objective of this experiment is to investigate the effects of SAP or SAO as ω-3 PUFA raw materials on production performance, egg quality, serum immunity, serum lipids, and fatty acid deposition patterns in the eggs of laying quails. Chinese yellow-feathered quails served [...] Read more.

The objective of this experiment is to investigate the effects of SAP or SAO as ω-3 PUFA raw materials on production performance, egg quality, serum immunity, serum lipids, and fatty acid deposition patterns in the eggs of laying quails. Chinese yellow-feathered quails served as the experimental subjects. A single-factor design was employed to randomly assign 1288 quails into four treatment groups, with seven replicates per treatment and 46 birds in each replicate. The groups included a control group (basal diet with no SAP), 1.6% SAP, 3.2% SAP, and 0.8% SAP + 0.3% SAO. The results indicate that: (1) Compared to the control group, the 0.8% SAP + 0.3% SAO group exhibited a reduction in daily egg-laying rate and egg mass, alongside an increased FCR; (2) the 3.2% SAP group enhanced egg yolk color, while the 1.6% SAP group reduced eggshell thickness, and the 0.8% SAP + 0.3% SAO group increased eggshell thickness; (3) compared to the control group, the 3.2% SAP group decreased total cholesterol (TC) and high-density lipoprotein cholesterol (HDL-C) levels in serum; the addition of either the 3.2% SAP or the 0.8% SAP + 0.3% SAO group significantly elevated quail serum immunoglobulin M (IgM) levels (p < 0.05); (4) in comparison to the control group, the addition of SAP or with SAP increased the contents of monounsaturated fatty acids (MUFA), polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA), and ω-3 PUFA in 56-day-old egg yolks while reducing the ω-6/ω-3 ratio (p < 0.05). These findings suggest that SAP as a source of ω-3 PUFA raw materials could improve quail health by improving lipid metabolism and immunity. 3.2% SAP was recommended as the optimal level to produce the enriched ω-3 PUFA quail eggs with the ω-3 PUFA ≥ 300 mg/100 g. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

14 pages, 10481 KB

Open AccessFeature PaperArticle

Multi-Omics Reveal the Improvements of Nutrient Digestion, Absorption, and Metabolism and Intestinal Function via GABA Supplementation in Weanling Piglets

by Yan Zeng, Hong Hu, Yiwen He, Zhiying Deng, Yiting Guo and Xihong Zhou

Animals 2024, 14(22), 3177; https://doi.org/10.3390/ani14223177 - 6 Nov 2024

Cited by 4 | Viewed by 2775

Abstract

The nonprotein amino acid γ-aminobutyric acid (GABA) can enhance intestinal function in piglets; however, the mechanisms involved are not yet fully understood. To explore the effects of GABA and its underlying mechanisms, weanling piglets were randomly assigned to three groups, receiving either a [...] Read more.

The nonprotein amino acid γ-aminobutyric acid (GABA) can enhance intestinal function in piglets; however, the mechanisms involved are not yet fully understood. To explore the effects of GABA and its underlying mechanisms, weanling piglets were randomly assigned to three groups, receiving either a basal diet or a basal diet supplemented with GABA (80 mg/kg or 120 mg/kg). The results demonstrated that dietary GABA improved growth performance and reduced diarrhea incidence (p < 0.05). Additionally, GABA supplementation decreased the serum and intestinal levels of pro-inflammatory cytokines (p < 0.05), and improved intestinal morphology. Multi-omics analyses were employed to explore the alterations caused by GABA supplementation and elucidate the related mechanisms. Microbiota profiling revealed improved beta-diversity and changes in the composition of ileal bacteria and fungi. Amino acid metabolism, lipid metabolism, and digestive processes were primarily enriched in the GABA group according to metabolomics analysis. A transcriptome analysis showed significant enrichment in ion transmembrane transport and nutrition absorption and digestion pathways in the ileum. Furthermore, increased lipase and trypsin activity, along with the elevated expression of tight junction proteins confirmed the beneficial effects of GABA on intestinal nutrient metabolism and barrier function. In conclusion, dietary 80 mg/kg GABA supplementation improved nutrient digestion and absorption and intestinal function in weanling piglets. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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11 pages, 248 KB

Open AccessArticle

The Optimal Dietary Sodium or Chloride Level of Broilers Fed a Corn–Soybean Meal Diet from 22 to 42 Days of Age

by Xiaoyan Cui, Feiyu Gao, Weiyun Zhang, Wei Wu, Liyang Zhang, Yun Hu, Shengchen Wang, Tingting Li and Xugang Luo

Animals 2024, 14(22), 3173; https://doi.org/10.3390/ani14223173 - 6 Nov 2024

Cited by 1 | Viewed by 2242

Abstract

The objective of this study was to assess the ideal dietary Na or Cl level for chickens from 22 to 42 d of age, utilizing a corn–soybean meal diet that maintains a 1:1 ratio of Na to Cl. At 22 d of age, [...] Read more.

The objective of this study was to assess the ideal dietary Na or Cl level for chickens from 22 to 42 d of age, utilizing a corn–soybean meal diet that maintains a 1:1 ratio of Na to Cl. At 22 d of age, a total of 288 male broilers of Arbor Acres were selected and randomly divided into one of six treatments. Each treatment contained eight replicates, with six chicks in each replicate. The control chicks were given a basic diet including 0.02% Na and 0.07% Cl without added Na or Cl, while the birds in other treatments were given diets containing different Na or Cl levels (0.13%, 0.19%, 0.25%, 0.31%, and 0.37%). With increasing dietary Na or Cl levels, linear (p < 0.0001) and quadratic (p < 0.0001) increases occurred in ADG, ADFI, and average daily water consumption, while F/G exhibited a linear (p < 0.0001) and quadratic (p < 0.0001) decrease from 22 to 42 d of age in broilers. Linear (p < 0.02) and quadratic increases (p < 0.03) were found in the heart indicator, serum Cl⁻ concentrations, and blood Na⁺ and Cl⁻ concentrations, whereas the kidney indicator, blood K⁺ concentrations, and serum K⁺, UA, and glucose (GLU) concentrations showed a linear (p < 0.04) and quadratic (p < 0.004) decrease with an increase in dietary Na or Cl levels. The excreta water content, liver indicator, and jejunal crypt depth also showed a linear increase (p < 0.02). The ideal levels of dietary Na or Cl were estimated to be 0.07% to 0.12%, based on the most suitable broken-line and asymptotic models (p ≤ 0.0002) using those sensitive indicators mentioned above. It was suggested that the suitable dietary Na or Cl level of 0.12% would adequately fulfill the Na or Cl metabolic demands of chickens from 22 to 42 d of age. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

17 pages, 20655 KB

Open AccessArticle

Dietary Isatidis Root Residue Improves Diarrhea and Intestinal Function in Weaned Piglets

by Zhong Chen, Zenghao Yan, Siting Xia, Kaijun Wang, Qi Han, Miao Zhou, Deqin Wang, Jie Yin and Yulong Yin

Animals 2024, 14(19), 2776; https://doi.org/10.3390/ani14192776 - 26 Sep 2024

Cited by 7 | Viewed by 2420

Abstract

Weaning stress can trigger diarrhea, cause intestinal damage, and disrupt the intestinal flora of piglets, ultimately resulting in retarded growth or even the death of the animals. Traditional Chinese medicine residues encompass numerous bioactive compounds and essential nutrients; however, their efficient utilization remains [...] Read more.

Weaning stress can trigger diarrhea, cause intestinal damage, and disrupt the intestinal flora of piglets, ultimately resulting in retarded growth or even the death of the animals. Traditional Chinese medicine residues encompass numerous bioactive compounds and essential nutrients; however, their efficient utilization remains a challenge. Consequently, our study sought to explore the impact of traditional Chinese medicine residues, specifically Isatidis Root residue (IRR), on the growth performance, intestinal function, and occurrence of weaning diarrhea in newly weaned piglets. Forty healthy, castrated Duroc × Landrace × Yorkshire males, weaned at 21 days old and exhibiting similar body conditions, were randomly allocated into five groups, with eight piglets in each group. The results indicated that the dietary inclusion of IRR at concentrations ranging from 0.5% to 4.0% notably decreased the incidence of diarrhea in weaned piglets compared to the control group (p < 0.05). Serum LDL-C and globulin (GLB) contents were reduced in response to dietary IRR concentrations (0.5% to 4.0%), while serum albumin (ALB) and albumin/globulin (A/G) contents were enhanced (p < 0.05). Dietary 0.5%, 1.0%, and 2.0% IRR resulted in significant increases in villus height (VH) and villus height/crypt depth (V/C) ratios in the jejunum, V/C ratios in the ileum, and the number of villi goblet cells both in the jejunum and ileum. IRR also led to a significant decrease in the crypt depth (CD) of the jejunum and ileum (p < 0.05). Furthermore, the expression of IL-6 in the jejunum was significantly increased in IRR-fed piglets (0.5% to 4.0%) (p < 0.05). IRR demonstrated inhibitory effects on harmful bacteria in the gastrointestinal microbiome, including Campylobacter, Actinobacillus minor, and Ralstonia pickettii, indicating its broad-spectrum bacteriostatic properties. In conclusion, dietary IRR alleviated diarrhea in weaned piglets and improved gut function and microbial compositions. Full article

(This article belongs to the Special Issue Dietary Components in Animal Nutrition: Favoring Sustainability, Welfare and Safety)

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