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Fishes
Volume 11
Issue 5
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Fishes, Volume 11, Issue 5 (May 2026) – 55 articles

Cover Story (view full-size image): Marine plastic pollution is an emerging environmental concern in the Mediterranean Sea, with microplastics increasingly being detected in commercially important fish species. This study investigates anthropogenic particle ingestion in Atlantic chub mackerel (Scomber colias) collected from the Saronikos Gulf, one of the most industrialized coastal areas in Greece. The results reveal the occurrence, characteristics, and composition of ingested anthropogenic particles, highlighting the predominance of small fragments and fibers. By linking particle ingestion with biological traits of the species, this study provides valuable insight into pollution exposure pathways in pelagic fish and contributes to ongoing efforts to assess the ecological implications of anthropogenic particle contamination in marine ecosystems and seafood resources. View this paper
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18 pages, 4212 KB  
Article
AHSC-Net: A Fish Pose Estimation Method for Intelligent Monitoring in Precision Aquaculture
by Xiaohong Peng, Ronghan Lu, Zhuohan Xiao and Xiaohan Chen
Fishes 2026, 11(5), 308; https://doi.org/10.3390/fishes11050308 - 21 May 2026
Viewed by 733
Abstract
In aquaculture, fish physiological information serves as the foundation for behavior recognition, precise feeding, and health monitoring. The acquisition of such information relies on accurate keypoint detection and pose estimation of the fish body. To address the challenges caused by inter-occlusion among fish [...] Read more.
In aquaculture, fish physiological information serves as the foundation for behavior recognition, precise feeding, and health monitoring. The acquisition of such information relies on accurate keypoint detection and pose estimation of the fish body. To address the challenges caused by inter-occlusion among fish schools and blurred keypoint boundaries in underwater environments, a novel fish pose estimation method based on the Adaptive-kernel Hybrid-center Structural Constraint Network (AHSC-Net) is proposed. Optimized specifically for the characteristics of fish poses, the proposed method effectively enhances detection accuracy and robustness in complex underwater scenarios. First, a Stochastic Local Centroid Sampling (SLCS) strategy is introduced to improve detection capability. By simulating centroid positions in occluded samples, this approach enhances the model’s ability to detect partially occluded fish. Next, a Spatial-Awareness Enhanced Pose Structural Constraint (SAPSC) is established through coordinate embedding and morphological constraints. It ensures the rationality of the predicted poses. Furthermore, an Adaptive Kernel Modulation Module (AKMM) is designed to dynamically adjust the Gaussian kernel distribution, effectively addressing challenges posed by underwater blurring and variations in fish scales. Experimental results demonstrate that AHSC-Net achieves 92.0% AP and 94.6% AR on a self-constructed largemouth bass dataset, outperforming state-of-the-art methods such as HRNet, HigherHRNet, DEKR, and YOLO-Pose. This study presents a fish pose estimation method that provides effective technical support for automated and precise monitoring in aquaculture. Full article
(This article belongs to the Special Issue Computer Vision Applications for Fisheries and Aquaculture)
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13 pages, 1010 KB  
Article
Comparative Analysis of Growth Patterns and Sexual Dimorphism of Scylla paramamosain in Pond Culture
by Jiahui Liu, Ronghua Li, Yang Jiang, Yun Hu, Zhuang Li, Qingyang Wu, Changkao Mu, Weiwei Song, Chunlin Wang and Ce Shi
Fishes 2026, 11(5), 307; https://doi.org/10.3390/fishes11050307 - 21 May 2026
Viewed by 763
Abstract
To investigate the growth patterns and sexual differences in pond-cultured mud crabs (Scylla paramamosain), this study measured eight growth patterns in pond-cultured S. paramamosain aged 1 to 5 months, including internal carapace width (ICW), abdomen width (AW), body height (BH), carapace [...] Read more.
To investigate the growth patterns and sexual differences in pond-cultured mud crabs (Scylla paramamosain), this study measured eight growth patterns in pond-cultured S. paramamosain aged 1 to 5 months, including internal carapace width (ICW), abdomen width (AW), body height (BH), carapace length (CL), propodus length (PL), merus length (ML), first periopod merus length (1PML), and body weight (BW), with measurements taken monthly. The growth patterns for females, males, and a mixed-sex group were fitted using the following three growth curve models: Logistic, Gompertz, and von Bertalanffy. The fitting results indicate that the optimal growth model for male S. paramamosain is the Logistic model, while the optimal growth model for female S. paramamosain is the von Bertalanffy model. The predicted growth inflection points and inflection weight for male S. paramamosain are 3.15 months and 155.00 g, respectively; for female S. paramamosain, the predicted growth inflection point and inflection weight are 4.25 months and 228.71 g, respectively; and for mixed-sex S. paramamosain, the growth inflection point and inflection weight are 3.22 months and 151.80 g, respectively. Males achieve a rapid growth period earlier (at 3–4 months of age) than females, with the weight of male crabs significantly greater than that of females at four months of age (p < 0.05). Males exhibit slow weight gain from the fourth to the fifth month, while females demonstrate a rapid weight gain rate during the same period. These results provided a theoretical basis and reference for the refined pond culture of S. paramamosain. Full article
(This article belongs to the Special Issue Biology and Culture of Marine Invertebrates)
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19 pages, 9494 KB  
Article
Effects of Strontium Marking on Otolith Elemental Deposition, Digestive Enzymes, and Antioxidant System in Juvenile Japanese Flounder (Paralichthys olivaceus)
by Jiahui Zhang, Siyang Li, Jun Zhang, Jinming Zhang, Tianyi Li, Jianhua Li, Jun Yang and Yan Wang
Fishes 2026, 11(5), 306; https://doi.org/10.3390/fishes11050306 - 20 May 2026
Viewed by 734
Abstract
Japanese flounder (Paralichthys olivaceus) is an economically important species in China’s marine fishery industry. However, due to long-term intensive fishing, its wild population has declined sharply. Artificial stock enhancement has become a core measure for restoring its resources. This study aimed [...] Read more.
Japanese flounder (Paralichthys olivaceus) is an economically important species in China’s marine fishery industry. However, due to long-term intensive fishing, its wild population has declined sharply. Artificial stock enhancement has become a core measure for restoring its resources. This study aimed to investigate the effects of different immersion durations and concentrations of SrCl2 solution (10, 20, 40, 80 mg/L) on strontium (Sr) deposition in the otoliths of P. olivaceus, and to systematically evaluate the impacts of Sr marking on the fish’s antioxidant capacity and digestive enzyme activity. The results showed that the otolith Sr/Ca ratio was positively correlated with marking concentration and duration; the optimal parameters were 40 mg/L for 4 days, with the Sr/Ca ratio returning to baseline after 30 days post-marking, and a 100% marking success rate. There were no significant differences in body length, body weight, or condition factor between the experimental groups and the control group (p > 0.05), but mortality was significantly increased in the 80 mg/L group. Digestive enzymes exhibited a dose-dependent response to Sr exposure, characterized by activation at low concentrations and inhibition at high concentrations; lipase was the most sensitive, with an inhibition threshold of 10 mg/L. Sr marking within the range of 20–40 mg/L for 4–8 days significantly activated the activity of T-AOC, CAT, GPx, and SOD (p < 0.05) and reduced MDA content, indicating that the antioxidant system was activated without causing persistent oxidative damage. In conclusion, Sr marking is a safe and efficient method for otolith marking in Paralichthys olivaceus. The recommended protocol is immersion in a 40 mg/L SrCl2 solution for 4 days, followed by a 30-day recovery period in clean seawater before being used for stock enhancement evaluation. This study provides a scientific basis and technical support for assessing the effectiveness of stock enhancement in P. olivaceus. Full article
(This article belongs to the Special Issue Adaptive Fisheries Management in East Asia: Navigating Climate Change, POPs, and Microplastics)
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23 pages, 2136 KB  
Article
Natural Dietary Supplementation with Elionurus muticus Essential Oil Enhances Growth Performance and Modulates Physiological Responses to Transport Stress in Nile Tilapia
by Aline da Silva Rocha, José Fernando Bibiano Melo, David Ramos da Rocha, Rafael Carvalho da Silva, Rafael Silva Marchão, Alane Pains Oliveira do Monte, Gabriela Cristina da Silva Santos, Samantha Chung, Leandro Licursi de Oliveira, Denise Schmidt, Rodrigo Fortes-Silva and Carlos Eduardo Copatti
Fishes 2026, 11(5), 305; https://doi.org/10.3390/fishes11050305 - 20 May 2026
Cited by 1 | Viewed by 952
Abstract
This study evaluated the effects of dietary Elionurus muticus essential oil (EMEO) on growth performance, physiological responses, and resistance to car transport stress in Nile tilapia (Oreochromis niloticus). Fish were fed experimental diets for 60 days and subsequently subjected to 6 [...] Read more.
This study evaluated the effects of dietary Elionurus muticus essential oil (EMEO) on growth performance, physiological responses, and resistance to car transport stress in Nile tilapia (Oreochromis niloticus). Fish were fed experimental diets for 60 days and subsequently subjected to 6 h of transport stress. Five diets were tested: 0.00 (control), 0.25, 0.50, 1.00, and 1.50 mL EMEO kg−1, in triplicate (10 fish per 500 L tank; stocking density 0.4 kg L−1). Citral was the major EMEO compound (73.91%). Increasing dietary EMEO levels improved growth performance and reduced the feed conversion ratio. Before transport, EMEO supplementation increased erythrocyte counts and plasma glucose levels, while reducing hematocrit and hepatic aspartate aminotransferase (AST) activity (p < 0.05). After transport, plasma glucose, hematocrit, and hepatic AST values decreased, whereas hepatic glycogen and hemoglobin levels increased with higher EMEO inclusion (p < 0.05). Also, post-transport, EMEO-fed fish showed enhanced intestinal digestive enzyme activity (lipase and amylase) and antioxidant capacity (superoxide dismutase and ferric reducing antioxidant power) but increased protein carbonyl levels. Lipid peroxidation (malondialdehyde) was reduced at intermediate EMEO levels (p < 0.05). Histological analyses indicated no tissue damage and suggested improved liver and intestinal function with increasing EMEO inclusion. Overall, dietary supplementation with 1.00 mL EMEO kg−1 is recommended to enhance growth performance and metabolic adjustment and to improve physiological status to withstand transport stress in Nile tilapia. Full article
(This article belongs to the Special Issue Advances in Tilapia Aquaculture)
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23 pages, 305 KB  
Article
The Impact of Artificial Intelligence on the Resilience of China’s Fisheries Industry Chain: Evidence from Panel Data Analysis
by Wenjing Huang, Haoze Liang, Shiwei Xu, Jing Qin and Ekaterina Shebanova
Fishes 2026, 11(5), 304; https://doi.org/10.3390/fishes11050304 - 20 May 2026
Viewed by 908
Abstract
Marine fisheries are a cornerstone of food security, and artificial intelligence (AI) technology holds significant strategic importance for enhancing fishery industrial chain resilience. This study utilizes provincial panel data from China covering the period 2010–2024 to assess the level of fishery industrial chain [...] Read more.
Marine fisheries are a cornerstone of food security, and artificial intelligence (AI) technology holds significant strategic importance for enhancing fishery industrial chain resilience. This study utilizes provincial panel data from China covering the period 2010–2024 to assess the level of fishery industrial chain resilience across 29 provinces (excluding Qinghai, Tibet, Hong Kong, Macao and Taiwan), and employs a two-way fixed-effects model to investigate the mechanisms through which AI influences the resilience of the fisheries industry chain. The findings reveal the following: (1) AI has a significant positive impact on the resilience of the fisheries industry chain. For every one-unit increase in AI development level, the fishery industrial chain resilience increases by 0.470 units; (2) AI enhances fishery industrial chain resilience by promoting structural upgrades in the fisheries industry and improving resource allocation efficiency; (3) heterogeneity analysis indicates that the enabling effects of AI are more pronounced in samples characterized by well-developed digital infrastructure, a higher level of digital economic development, and those located in the eastern regions. This study provides new empirical evidence for understanding how AI empowers the resilience of the fisheries industry chain, and holds significant theoretical and practical value for formulating fisheries science and technology policies tailored to local conditions and for safeguarding national food security. Full article
(This article belongs to the Special Issue Advances in Fisheries Economics)
12 pages, 477 KB  
Article
Effects of Dietary Tryptophan on Growth, Protein Degradation, and Antioxidant Enzyme Activity in Juvenile Meagre (Argyrosomus regius)
by Margarida Saavedra, Ana Vasconcelos, Ana Catarina Matias, Florbela Soares, Marisa Barata and Pedro Pousão-Ferreira
Fishes 2026, 11(5), 303; https://doi.org/10.3390/fishes11050303 - 20 May 2026
Viewed by 789
Abstract
Tryptophan has been shown to affect fish feed intake and growth performance. Moreover, it is the precursor of several bioactive molecules such as serotonin, which can be converted into melatonin. Melatonin is a potent antioxidant that directly neutralises free radicals and reduces oxidative [...] Read more.
Tryptophan has been shown to affect fish feed intake and growth performance. Moreover, it is the precursor of several bioactive molecules such as serotonin, which can be converted into melatonin. Melatonin is a potent antioxidant that directly neutralises free radicals and reduces oxidative stress. Diets rich in tryptophan may contribute to reduced oxidative stress, potentially through its role as a precursor of serotonin and melatonin. In this study, three diets containing different contents of tryptophan: 0.5 (Trip1), 0.6 (Trip2) and 0.8% (Trip3), were tested in triplicate in 112-day-old meagre with an initial weight of 32.6 ± 3.4 g and 14.4 ± 0.5 cm length for 56 days. Although the results showed no significant differences for growth and FCR between treatments, there was a tendency toward increased growth and a decrease in FCR in meagre fed higher levels of tryptophan. The main protein degradation systems in the liver and white muscle were evaluated. The activity of the tested proteases in the muscle was unaffected by dietary tryptophan levels. A decrease in oxidative stress was also observed as the level of tryptophan in the diets increased, although not statistically significant. A trend of decreasing superoxide dismutase, catalase, and selenium-independent glutathione peroxidase levels in tryptophan-rich diets was also observed. Full article
(This article belongs to the Special Issue Aquaculture Feeds and Feed Additives—Toward Precise and Sustainable Nutrition)
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24 pages, 3755 KB  
Article
Annual and Spatial Variation in the Diet of Juvenile Pacific Cod in Mutsu Bay, Japan
by Anran Dong, Tetsuya Takatsu, Tomoya Ishikawa, Kenta Sasaki and Mitsuhiro Nakaya
Fishes 2026, 11(5), 302; https://doi.org/10.3390/fishes11050302 - 19 May 2026
Viewed by 620
Abstract
To evaluate Mutsu Bay as a nursery habitat for Pacific cod (Gadus macrocephalus Tilesius, 1810), we analyzed settled age-0 juveniles collected with a small bottom otter trawl over 10 years. The three stations with the highest juvenile densities were targeted, and prey-specific [...] Read more.
To evaluate Mutsu Bay as a nursery habitat for Pacific cod (Gadus macrocephalus Tilesius, 1810), we analyzed settled age-0 juveniles collected with a small bottom otter trawl over 10 years. The three stations with the highest juvenile densities were targeted, and prey-specific feeding intensity (SCIi), its sum (SCI), and the relative condition factor (Kn) were quantified, followed by examining their relationships with juvenile attributes and environmental variables. Diets varied among stations and shifted ontogenetically from small-sized calanoid copepods to larger planktonic and benthic prey. SCI was highest at stations where juveniles consumed medium-sized plankton (0.1–1.0 mg ind−1), including Calanus pacificus, Mesocalanus tenuicornis, Metridia pacifica, Anomura zoeae, and Euphausiacea furciliae, and lower where other prey dominated. High-SCI individuals were rarely observed, likely reflecting enhanced digestion at high temperatures near the upper habitat limit (~12 °C) and consistently low prey density independent of temperature. Kn increased with body size and SCI and tended to be higher in cooler water and closer to the bay mouth, suggesting coupled environmental and physiological constraints. These results suggest that after late May, juveniles may benefit from moving toward the bay mouth, where prey encounter rates are likely higher, including relatively larger prey, which may improve feeding opportunities and condition. Full article
(This article belongs to the Special Issue Ecology of Fish: Age, Growth, Reproduction and Feeding Habits)
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22 pages, 3372 KB  
Article
Multi-Class Marine Organism Detection Using Multi-Scale Attention-Enhanced YOLO11n
by Zehuan Bai, Haoxi Mao, Junliang Xu, Na Lv and Yiran Liu
Fishes 2026, 11(5), 301; https://doi.org/10.3390/fishes11050301 - 19 May 2026
Viewed by 481
Abstract
Monitoring marine organisms plays a vital role in biodiversity conservation, marine environmental management, and fisheries resource management. However, the underwater environment is often low-light and turbid, leading to indistinct target boundaries. Moreover, the wide variety of marine organisms—with significant differences in color, scale, [...] Read more.
Monitoring marine organisms plays a vital role in biodiversity conservation, marine environmental management, and fisheries resource management. However, the underwater environment is often low-light and turbid, leading to indistinct target boundaries. Moreover, the wide variety of marine organisms—with significant differences in color, scale, texture, and morphology—can easily result in missed detections. To address these challenges, this paper proposes a multi-class marine organism detection method using multi-scale attention-enhanced You Only Look Once 11 nano (YOLO11n). The method incorporates the Convolutional Block Attention Module (CBAM) into the YOLO11n network, enabling the model to better focus on key feature regions while effectively suppressing background noise interference in complex marine environments. In addition, the model is trained using the Complete Intersection over Union (CIoU) loss function, which enhances bounding box regression accuracy, especially in handling targets of varying scales. The effectiveness of the proposed method is validated on the publicly available BrackishMOT dataset. The proposed model achieves an overall mAP@0.5 of 0.481, computed as the average AP across six organism categories. Category-wise results indicate stronger performance on visually distinguishable targets, such as Jellyfish, Starfish, and Small fish, with AP values of 0.808, 0.678, and 0.677, respectively. In contrast, performance remains limited for rare or visually ambiguous categories. These results suggest that the proposed method is effective for multi-class marine organism detection, particularly when discriminative visual features are present. Full article
(This article belongs to the Special Issue Computer Vision Applications for Fisheries and Aquaculture)
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34 pages, 21357 KB  
Article
A Novel Dual-Index Analysis Method for Quantifying Fish School Feeding Intensity Using Average Swimming Speed and Feeding Aggregation Speed
by Bo Jia, Xiaochan Wang, Yinyan Shi, Jinming Zheng, Jihao Wang, Zhen Xu, Xiaolei Zhang and Chengquan Zhou
Fishes 2026, 11(5), 300; https://doi.org/10.3390/fishes11050300 - 18 May 2026
Viewed by 796
Abstract
Accurate identification and quantitative assessment of fish feeding intensity are pivotal for enhancing aquaculture production efficiency. Currently, feeding intensity is mainly assessed based on fish school feeding images with a single feature, overlooking the interdependencies between individual fish and the fish school’s behavior. [...] Read more.
Accurate identification and quantitative assessment of fish feeding intensity are pivotal for enhancing aquaculture production efficiency. Currently, feeding intensity is mainly assessed based on fish school feeding images with a single feature, overlooking the interdependencies between individual fish and the fish school’s behavior. Therefore, this paper presents a method based on detecting individual fish heads to characterize the feeding aggregation speed and the average swimming speed of the fish school, thereby quantifying the fish school’s feeding intensity. First, the improved YOLOv11n-ALL model was employed to detect individual fish heads, resulting in improved detection performance, increasing inference speed, and reducing computational complexity. Additionally, feeding aggregation speed and average swimming speed indices for fish schools were constructed by combining the YOLOv11n-ALL model with the ByteTrack algorithm to track and extract the centers of individual fish heads’ detection boxes. Finally, the fish school feeding kinetic energy was assessed using the feeding aggregation speed and average swimming speed dual indices, and the fish school feeding intensity levels were classified according to the feeding kinetic energy. Experimental results reveal that the improved YOLOv11n-ALL model achieved an average detection precision (mAP50) of 94.13% for detecting fish heads, reduced the parameter count by 22.09%, and exhibited a computational complexity of 6.4 GFLOPs. Furthermore, the classification model of fish school feeding intensity, quantified by the dual indices of average swimming speed and feeding aggregation speed, achieved a detection accuracy of 97.41%. This method digitizes detection results, enabling rapid classification of fish school feeding intensity and demonstrating its effectiveness for feeding intensity assessment and the development of scientific feeding strategies. Full article
(This article belongs to the Special Issue Computer Vision Applications for Fisheries and Aquaculture)
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21 pages, 3927 KB  
Article
Preliminary Study Finds LEDs, UV Lights, and C-Type Hooks May Reduce Sustainability in Aegean Small-Scale Fisheries
by Yakup Kaska, Doğan Sözbilen, Melissa Ana Vezard, Paolo Casale, Muharrem Hakan Kaykaç, Zafer Tosunoğlu and Earl Possardt
Fishes 2026, 11(5), 299; https://doi.org/10.3390/fishes11050299 - 18 May 2026
Viewed by 978
Abstract
Marine coastal ecosystems provide a variety of habitats for biodiversity. However, they are affected by bycatch, the unintentional capture of non-target species during fishing operations such as gillnets, trammel nets, and longlines. To mitigate bycatch, modifications such as LED and UV lights in [...] Read more.
Marine coastal ecosystems provide a variety of habitats for biodiversity. However, they are affected by bycatch, the unintentional capture of non-target species during fishing operations such as gillnets, trammel nets, and longlines. To mitigate bycatch, modifications such as LED and UV lights in gillnets and trammel nets and C-type (circle) hooks in longlines have been studied worldwide. Yet, studies remain limited in Türkiye. This study aimed to evaluate the effectiveness of these gear modifications in small-scale fisheries along the Aegean coast of Türkiye. Paired trials were conducted to compare standard (control) and modified (LED, UV, or C-type hook) fishing gears. Trials resulted in four sea turtles caught in LED and UV nets with no significance. Other vulnerable species caught in UV trammel nets showed significance. Overall, modified gears showed a significant reduction in commercial species catch, while increasing non-target species captures. These preliminary findings contradict much of the literature, which generally reports these modifications as effective bycatch reduction tools. The results emphasize the necessity of developing regionally adapted gear modifications and conducting more extensive experiments to validate their performance. The implementation of locally optimized bycatch mitigation tools may help achieve a balance between marine conservation and the socioeconomic sustainability of small-scale fisheries. Full article
(This article belongs to the Special Issue Sustainable Management of Small-Scale and Data-Limited Fisheries: Diagnosis and Strategies)
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29 pages, 9040 KB  
Article
Integrated Laser Imaging for Fusiform Fish Measurement in Aquaculture
by Shuxian Wang, Shengmao Zhang, Yongchuang Shi, Zuli Wu and Tianfei Cheng
Fishes 2026, 11(5), 298; https://doi.org/10.3390/fishes11050298 - 18 May 2026
Cited by 1 | Viewed by 715
Abstract
This paper details the implementation of an integrated engineering framework for the real-time assessment of pose and size in fusiform fish, utilizing laser-camera technology. The design, comprising a camera and laser emitter, leverages laser triangulation for accurately measuring distances between key points, providing [...] Read more.
This paper details the implementation of an integrated engineering framework for the real-time assessment of pose and size in fusiform fish, utilizing laser-camera technology. The design, comprising a camera and laser emitter, leverages laser triangulation for accurately measuring distances between key points, providing a reliable baseline for data comparison. Enhanced with the yolov7 model backbone, it includes detection and segmentation features, enabling precise image instance segmentation of fish and laser lines. The system’s dual-network structure, which combines fully connected regression and DSNT-MobileFaceNet networks, efficiently identifies six crucial landmarks on fish—an essential step for detailed pose analysis. This method facilitates the accurate determination of two-dimensional fish posture by analyzing the relative positions of these landmarks. A notable capability of this system is its ability to infer depth information from laser lines on the fish’s body, aiding in the accurate measurement of dimensions such as body length and depth. Empirical results demonstrate the system’s effectiveness, with high mean Average Precision (mAP) values for both object detection (0.9560 for fish, 0.8550 for laser lines) and segmentation (0.9740 for fish, 0.8420 for laser lines). The DSNT-MobileFaceNet network, in particular, shows excellent fitting accuracy with an R2 value of 0.9170. The deep learning model achieves an average error rate of 7.75% in detecting fish data, markedly improving upon the baseline error rate of 14.70%. Overall, this study confirms the proposed system’s capability in accurately assessing fish pose and size. As a rigorous proof of concept validated in a controlled laboratory environment, this work establishes a foundational framework for non-invasive morphological monitoring, suggesting its future applicability in marine biology and aquaculture. Full article
(This article belongs to the Special Issue Computer Vision Applications for Fisheries and Aquaculture)
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17 pages, 2547 KB  
Review
Status and Development Potential of Bellamya Aquaculture in Asia: Ecology, Integrated Farming Models, and High-Value Utilization
by Wu Jin, Jianwei Liu, Benhe Ma, Xianhui Pan, Xueyan Ma, Xiaojuan Cao and Haibo Wen
Fishes 2026, 11(5), 297; https://doi.org/10.3390/fishes11050297 - 16 May 2026
Viewed by 662
Abstract
Freshwater snails, specifically those belonging to the genus Bellamya, are increasingly recognized as important components of sustainable aquaculture and aquatic ecosystem management. This review synthesizes current knowledge on their ecological roles, aquaculture practices, utilization, and associated risks to evaluate their potential as [...] Read more.
Freshwater snails, specifically those belonging to the genus Bellamya, are increasingly recognized as important components of sustainable aquaculture and aquatic ecosystem management. This review synthesizes current knowledge on their ecological roles, aquaculture practices, utilization, and associated risks to evaluate their potential as a multifunctional resource. Available evidence shows that Bellamya species function as bioindicators of environmental change and contribute to water purification through grazing, nutrient cycling, and interactions with aquatic plants. In aquaculture, diverse production systems, including rice–snail co-culture and pond-based farming, have been developed, demonstrating high resource-use efficiency and economic value. In addition to their nutritional importance as a protein source, freshwater snails provide opportunities for value-added products in food, biomaterials, and health-related applications. However, challenges remain, including parasite transmission, the bioaccumulation of environmental pollutants, genetic resource degradation, and ecological carrying capacity constraints under intensive farming. Future development depends on advances in breeding, nutrition, and intelligent farming technologies, as well as improved environmental monitoring and regulatory frameworks. Overall, freshwater snail aquaculture represents a promising pathway for integrating food production with ecosystem restoration, but its sustainable expansion requires coordinated efforts in research, management, and industry development. Full article
(This article belongs to the Special Issue Advances in Shellfish Aquaculture)
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14 pages, 3343 KB  
Article
Identification of Macrophage Migration Inhibitory Factor in Mytilus coruscus and Its Role in Methylation During Antibacterial Immunity
by Xuechun Wang, Chentenghong Yuan, Xirui Si, Pengzhi Qi and Bin Shen
Fishes 2026, 11(5), 296; https://doi.org/10.3390/fishes11050296 - 15 May 2026
Viewed by 424
Abstract
Macrophage migration inhibitory factor (MIF) is broadly acknowledged as a central pro-inflammatory regulator, owing to its multifaceted functions including immune cell recruitment, initiation and amplification of pro-inflammatory cytokine cascades, enhancement of macrophage viability, facilitation of macrophage polarization toward a pro-inflammatory state, and attenuation [...] Read more.
Macrophage migration inhibitory factor (MIF) is broadly acknowledged as a central pro-inflammatory regulator, owing to its multifaceted functions including immune cell recruitment, initiation and amplification of pro-inflammatory cytokine cascades, enhancement of macrophage viability, facilitation of macrophage polarization toward a pro-inflammatory state, and attenuation of glucocorticoid-mediated immunosuppression. However, functional investigations of MIF in Mytilus coruscus remain limited. In this study, we identified the MIF gene in M. coruscus, and bioinformatic analyses revealed that the gene encodes a 115-amino-acid polypeptide that exhibits close phylogenetic affinity with MIF homologs from other mollusks. McMIF was predominantly expressed in immune-related tissues, with notably high expression levels in the digestive gland. Upon Vibrio alginolyticus infection, both the mRNA and protein levels of McMIF were significantly upregulated, suggesting that McMIF is involved in the antibacterial immune response of M. coruscus. Meanwhile, the m6A modification level of McMIF was markedly reduced following infection, suggesting a potential relationship between m6A modification and the antibacterial immune function of MIF. Furthermore, knockdown of McMIF followed by LPS stimulation led to an increased level of apoptosis in digestive gland cells, suggesting that McMIF is involved in the inhibition of apoptosis induced by immune stimulation. Collectively, these findings provide insights into the immunological characteristics of McMIF in M. coruscus. Full article
(This article belongs to the Special Issue Advances in the Immunology of Aquatic Animals)
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23 pages, 21519 KB  
Article
Bioaccumulation of Trace Elements in the Most Commercial Fish in the Southern Black Sea and Risk Estimates Related to Their Consumption
by Levent Bat, Süleyman Özdemir, Zekiye Birinci Özdemir and Ayşah Öztekin
Fishes 2026, 11(5), 295; https://doi.org/10.3390/fishes11050295 - 15 May 2026
Viewed by 916
Abstract
This study evaluates the accumulation of trace elements (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb) in the muscle tissues of six commercially important fish species (Scophthalmus maximus, Merlangius merlangus, Mullus ponticus, Trachurus mediterraneus [...] Read more.
This study evaluates the accumulation of trace elements (Al, Cr, Mn, Fe, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb) in the muscle tissues of six commercially important fish species (Scophthalmus maximus, Merlangius merlangus, Mullus ponticus, Trachurus mediterraneus, Engraulis encrasicolus, and Sprattus sprattus) harvested from multiple locations across the Sinop and Samsun coasts of the southern Black Sea during the 2023–2025 fishing seasons to assess potential human health risks. Element concentrations were quantified using inductively coupled plasma mass spectrometry (ICP-MS) and compared against national and international food safety standards. Results indicated that essential elements, particularly Fe and Zn, exhibited the highest concentrations, while Co and potentially toxic elements (Cd, Pb, As, and Hg) remained at lower levels. Although significant geographical variations in accumulation were observed between sampling locations, inter-species differences were relatively minor. Human health risk assessments, including Estimated Daily Intake (EDI), Target Hazard Quotient (THQ), and Carcinogenic Risk (CR), revealed that all THQ values were well below 1, indicating no non-carcinogenic concerns. Furthermore, CR values for As, Cr, and Pb fell within the acceptable range (10−6 to 10−4) defined by the U.S. EPA. Consequently, fish consumption from these regions poses no unacceptable risk, though localized element elevations suggest a need for enhanced environmental monitoring of pollution sources. Full article
(This article belongs to the Special Issue Stress Ecology of Aquatic Animals)
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18 pages, 3493 KB  
Article
Gut Microbiota and Metabolome Differences Between Fast- and Slow-Growing Brass Gudgeon (Coreius heterodon)
by Yafan Dai, Leiming Zhang, Xingyu Ma, Bing Xie, Xueying Pei, Xiaolan Shi, Jie Mei, Tao Wang, Guoqin Zhou and Wei Liu
Fishes 2026, 11(5), 294; https://doi.org/10.3390/fishes11050294 - 15 May 2026
Viewed by 462
Abstract
The gut microbiota plays a crucial role in regulating fish growth. In this study, we performed 16S rRNA sequencing and metabolomics to compare the gut microbiota and metabolic profiles of fast-growing (FG) and slow-growing (SG) brass gudgeon (Coreius heterodon) from the [...] Read more.
The gut microbiota plays a crucial role in regulating fish growth. In this study, we performed 16S rRNA sequencing and metabolomics to compare the gut microbiota and metabolic profiles of fast-growing (FG) and slow-growing (SG) brass gudgeon (Coreius heterodon) from the same family, reared under identical conditions for 12 months. Our results revealed that there was no significant difference in the overall gut microbiota structure between FG and SG groups, but significant differences were observed at specific phylum and genus levels. The FG group harbored a greater abundance of potential probiotics (e.g., Prevotella, Lactobacillus, and Lachnospiraceae NK4A136_group), while opportunistic pathogens such as Klebsiella and Pseudomonas were less abundant. Metabolomics analysis identified 136 differential metabolites, among them, 61 were upregulated and 75 were downregulated in the FG group, with higher levels of phosphatidylcholine, acylcarnitine, and amino acid derivatives in the FG group. KEGG pathway analysis showed enrichment of butanoate metabolism, tryptophan metabolism, and pyrimidine metabolism in the FG group. Spearman correlation analysis indicated that specific gut microbiota was significantly correlated with metabolites involved in energy metabolism, gut homeostasis, and oxidative balance. These findings revealed associations between specific gut microbiota, gut metabolites, and growth performance in brass gudgeon. Although overall community structure did not differ significantly between groups, the compositional and metabolic shifts observed suggest that the gut microbiota–metabolite association might be linked to growth variation. This study provided new insights into the microbiota–metabolite–growth axis of brass gudgeon and offers valuable reference information for the development of specialized probiotic feeds for this species. Full article
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23 pages, 4364 KB  
Article
Integrative Transcriptome and GWAS Analyses Reveal Growth-Associated Molecular Architecture in Pacific Abalone (Haliotis discus hannai)
by Hyejung Jung, Eun Soo Noh, Hyejin Kim, Hee-Bok Park, Young-Su Seo, In Jun Hwang, Hee Jeong Kong, Jung-Ha Kang and Jungwook Park
Fishes 2026, 11(5), 293; https://doi.org/10.3390/fishes11050293 - 15 May 2026
Viewed by 733
Abstract
Pacific abalone (Haliotis discus hannai) is a widely cultured and economically important abalone species in aquaculture yet improving growth performance remains a major challenge for stable production. To clarify the molecular architecture associated with growth performance in Pacific abalone, we integrated [...] Read more.
Pacific abalone (Haliotis discus hannai) is a widely cultured and economically important abalone species in aquaculture yet improving growth performance remains a major challenge for stable production. To clarify the molecular architecture associated with growth performance in Pacific abalone, we integrated transcriptome and genome-wide association study (GWAS) data from high-growth and low-growth groups showing significant growth differences. Transcriptome profiles from hepatopancreas and mantle tissues were used to construct a co-expression network of 43,125 genes, summarized into 22 modules associated with tissue specificity and growth-related variation. In parallel, analysis of a custom 60K SNP array identified 67 significant growth-associated SNPs. Integration of these GWAS signals into the co-expression network revealed a core module most strongly correlated with growth index and enriched for SNP-derived candidate genes. Functional enrichment indicated that the core module was associated with proteostasis and growth-related signaling pathways, including insulin, Ras, and MAPK signaling. Protein–protein interaction analysis further identified 11 hub genes with high intramodular connectivity and direct interactions with SNP-derived genes, most of which participate in receptor-mediated and intracellular growth-regulatory functions. These findings provide an integrated molecular framework for growth performance in Pacific abalone and candidate targets for future molecular breeding strategies. Full article
(This article belongs to the Special Issue Applications of Genome-Based Technologies in Aquaculture)
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11 pages, 743 KB  
Article
Preference for Natural Stimuli in Juvenile Guppies
by Chiara Varracchio, Cristiano Bertolucci, Giorgio Bertorelle and Tyrone Lucon-Xiccato
Fishes 2026, 11(5), 292; https://doi.org/10.3390/fishes11050292 - 14 May 2026
Viewed by 460
Abstract
Drawing on the well-documented preference for natural and highly biodiverse environments in humans, it has been proposed that other animals may also recognise and show interest in natural stimuli. If widespread across animals, such a preference could have important evolutionary and welfare implications. [...] Read more.
Drawing on the well-documented preference for natural and highly biodiverse environments in humans, it has been proposed that other animals may also recognise and show interest in natural stimuli. If widespread across animals, such a preference could have important evolutionary and welfare implications. However, we currently lack investigations of this preference outside humans. To begin filling this gap, we examined whether juvenile guppies (Poecilia reticulata) exhibit a spontaneous preference for natural stimuli. In a dichotomous choice test, guppies were given the opportunity to choose between an environment containing live aquatic plants and one with structurally similar, in shape and size, artificial plants. After habituation to the testing apparatus, guppies spent significantly more time in the environment with live plants, suggesting a preference for natural stimuli. This supports the idea that, beyond humans, other animals may also be capable of recognizing and responding to natural stimuli. Full article
(This article belongs to the Section Physiology and Biochemistry)
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28 pages, 10868 KB  
Article
Habitat Use, Residency, and Connectivity of Bull Sharks (Carcharhinus leucas) in the Bazaruto Seascape, Mozambique
by Stephanie K. Venables, Lukas Müller, Christoph A. Rohner, Andrea D. Marshall, Jimmy van Rijn, Nilza de Catarina, John D. Filmalter and Ryan Daly
Fishes 2026, 11(5), 291; https://doi.org/10.3390/fishes11050291 - 14 May 2026
Cited by 1 | Viewed by 2120
Abstract
Bull sharks (Carcharhinus leucas) are highly mobile coastal predators, whose broad movements complicate conservation planning, particularly in the Western Indian Ocean, where key habitats remain poorly understood. Using passive acoustic telemetry, this study assessed the habitat use, residency, and regional connectivity [...] Read more.
Bull sharks (Carcharhinus leucas) are highly mobile coastal predators, whose broad movements complicate conservation planning, particularly in the Western Indian Ocean, where key habitats remain poorly understood. Using passive acoustic telemetry, this study assessed the habitat use, residency, and regional connectivity of adult bull sharks within the Bazaruto Seascape, Mozambique, evaluating the area’s ecological role within a wider movement network. Sharks tagged around Bazaruto exhibited high residency (overall Rmax = 0.45) and year-round presence, with core habitat concentrated along seaward reef systems and inshore areas off the San Sebastian Peninsula. Although most core habitat areas (82%) overlapped with existing marine protected areas, important habitats extended beyond protected area boundaries. In addition to supporting resident individuals, the seascape functioned as a seasonal waypoint within a regional movement corridor, with transient sharks exhibiting short periods (<5 days) of seasonal residency (primarily between May and November). Movement patterns revealed strong connectivity with other aggregation sites along the southeast African coast and occasional long-distance movements across the Mozambique Channel to Madagascar and beyond. These findings demonstrate that the Bazaruto Seascape provides both critical habitat and acts as a connectivity node for resident and transient bull sharks, highlighting the need for conservation strategies that combine local spatial protection with broader regional and transboundary management frameworks. Full article
(This article belongs to the Special Issue Habitat as a Template for Life Histories of Fish)
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20 pages, 2315 KB  
Article
Age and Growth of Pacific Sardine (Sardinops sagax) off the U.S. Pacific Coast, 2012–2021
by Kelsey C. James, Jonathan M. Walker, Brittany D. Schwartzkopf, Emmanis Dorval and Brad E. Erisman
Fishes 2026, 11(5), 290; https://doi.org/10.3390/fishes11050290 - 14 May 2026
Viewed by 1060
Abstract
Pacific sardine (Sardinops sagax) are an economically important forage fish in the Northeast Pacific Ocean that undergo large changes in abundance over decadal scales and exhibit high individual variation in somatic growth. Past studies have suggested that somatic growth in Pacific [...] Read more.
Pacific sardine (Sardinops sagax) are an economically important forage fish in the Northeast Pacific Ocean that undergo large changes in abundance over decadal scales and exhibit high individual variation in somatic growth. Past studies have suggested that somatic growth in Pacific sardine may be density-dependent and vary regionally in response to environmental conditions. We analyzed somatic growth in Pacific sardine off the U.S. Pacific Coast during the recent period of low abundance (2012–2021) and compared the results to those of previous studies to evaluate evidence of spatial or temporal variation in growth. Sampled fish (n = 3228) ranged in length from 30 to 291 mm SL and in age from 0 to 9 years and displayed high individual variation in length-at-age and age-at-length. Length-at-age data were best explained by the von Bertalanffy growth model, and sample distribution simulations showed the dataset to be robust and unbiased. Estimated growth parameters (L∞ = 243, K = 0.795, t0 = −0.638) were consistent with an opportunistic life history strategy characterized by rapid growth, early maturation, and a short lifespan. While the estimated growth rate (K) was higher than in a previous study conducted during a period of high abundance and indicated that growth may be density-dependent, the parameter estimates from the previous study were influenced by sample distribution bias. Similarly, differences in study region, season, collection method, aging methods, and other factors precluded any definitive conclusions on the source of reported differences in growth patterns among studies. Full article
(This article belongs to the Special Issue Ecology of Fish: Age, Growth, Reproduction and Feeding Habits)
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21 pages, 1017 KB  
Review
Monoterpenes as Natural Anesthetics to Mitigate Stress in Fish: Advances Using the Zebrafish Larvae Model
by Raquel S. F. Vieira, Cláudia A. Rocha, Carlos A. S. Venâncio and Luís M. Félix
Fishes 2026, 11(5), 289; https://doi.org/10.3390/fishes11050289 - 13 May 2026
Cited by 1 | Viewed by 1455
Abstract
During production, fish are exposed to multiple environmental, physiological, and physical stressors, which compromise development, productivity, and welfare and urge the implementation of effective and safe stress-mitigating strategies, particularly during early developmental stages. Larval zebrafish (Danio rerio) constitute a powerful model [...] Read more.
During production, fish are exposed to multiple environmental, physiological, and physical stressors, which compromise development, productivity, and welfare and urge the implementation of effective and safe stress-mitigating strategies, particularly during early developmental stages. Larval zebrafish (Danio rerio) constitute a powerful model for studying acute stress responses due to the numerous advantages they offer, such as developmental transparency, a conserved hypothalamic–pituitary–interrenal (HPI) axis, and suitability for high-throughput screening. This review examines the potential of natural monoterpenes as stress-reducing compounds and compares their performance with conventional synthetic anesthetics. Evidence from vortex-flow stress paradigms, behavioral profiling and biochemical assays shows that acute stress in zebrafish larvae triggers metabolic disruption, behavioral hyperactivity and enzyme imbalance, with cortisol responses depending on stimulus intensity. Monoterpenes such as thymol and menthol consistently reduce stress-induced hyperactivity, support redox homeostasis and display favorable safety profiles at low doses and short exposures. Nevertheless, as research into these substances is still recent, evidence of any potential adverse effects is still limited. Although individual monoterpenes may act on different subsets of molecular targets, their multimodal mechanisms, including gamma-aminobutyric acid (GABA)ergic enhancement, voltage-gated ion channel and transient receptor potential (TRP) modulation, suggest broader and potentially safer actions compared to single-target anesthetics as tricaine methane sulfonate (MS-222). Collectively, these findings suggest that monoterpenes offer promising natural alternatives for stress mitigation in aquaculture and the refinement of research procedures involving early life stages. Full article
(This article belongs to the Special Issue Fish Health and Welfare in Aquaculture and Research Settings)
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23 pages, 3387 KB  
Article
Revealing Ontogenetic Vertical Migration in Deep-Sea Grenadiers (Macrouridae) from the Southwestern Atlantic Through Otolith Microchemistry
by César Santificetur, Rodolfo Miguel Silva, Ana Méndez, Jorge Pisonero and Alberto Teodorico Correia
Fishes 2026, 11(5), 288; https://doi.org/10.3390/fishes11050288 - 12 May 2026
Viewed by 895
Abstract
Understanding the life-history strategies of deep-sea fishes is essential for improving ecological knowledge and informing conservation efforts. Using otolith microchemistry, this study reconstructed the ontogenetic movement patterns of four grenadier species (Nezumia aequalis, Hymenocephalus billsam, Coelorinchus marinii, and Malacocephalus [...] Read more.
Understanding the life-history strategies of deep-sea fishes is essential for improving ecological knowledge and informing conservation efforts. Using otolith microchemistry, this study reconstructed the ontogenetic movement patterns of four grenadier species (Nezumia aequalis, Hymenocephalus billsam, Coelorinchus marinii, and Malacocephalus occidentalis) caught in the continental slope off southern Brazil (Southwestern Atlantic). Elemental signatures (Ba:Ca, Sr:Ca, Li:Ca, Mg:Ca, Mn:Ca, Ni:Ca, Cu:Ca, and Zn:Ca) were quantified along core-to-edge transects of sagittal otoliths using Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS). Ontogenetic shifts were identified using change-point detection (PELT). A general decline in elemental incorporation with age was observed across species, consistent with ontogenetic physiological regulation. Species-specific multi-elemental patterns suggest distinct ecological strategies. Nezumia aequalis exhibited an abrupt decline in Ba:Ca, indicating an early-life environmental shift. Hymenocephalus billsam showed increasing Ba:Ca and Sr:Ca profiles, consistent with continued use of pelagic-associated water masses. Coelorinchus marinii and Malacocephalus occidentalis displayed more complex patterns, with the latter showing pronounced Ba:Ca and Zn:Ca peaks that may reflect mid-life habitat shifts or physiological events. Mn:Ca ratios differed between pelagic and demersal species. Otolith microchemistry combined with change-point analysis could provide insights into deep-sea fish ontogeny, although interpretations should consider both environmental and physiological influences. Full article
(This article belongs to the Special Issue Application of Otoliths in Fish Ecology and Fisheries)
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13 pages, 913 KB  
Article
Biochemical Properties of Collagen from Four Flatfish Species in Cold Area: In Relation to Physiological Temperature and Amino Acid Composition
by Tetsuro Sugai, Katsuji Morioka and Kohsuke Adachi
Fishes 2026, 11(5), 287; https://doi.org/10.3390/fishes11050287 - 12 May 2026
Viewed by 673
Abstract
Collagen (ASC) was purified from four flatfish species (Greenland halibut, blackfin flounder, stone flounder, and slime flounder) collected from a cold area in Japan to investigate their biochemical properties. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis pattern, Fourier transform infrared spectroscopy, and circular dichroism analysis [...] Read more.
Collagen (ASC) was purified from four flatfish species (Greenland halibut, blackfin flounder, stone flounder, and slime flounder) collected from a cold area in Japan to investigate their biochemical properties. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis pattern, Fourier transform infrared spectroscopy, and circular dichroism analysis revealed the typical structure of type I collagen. The denaturation temperature (Td) of ASC positively correlated with the physiological temperature (Tp) using previously reported data, which were obtained from fish (a total of 32 species). In this study, the lowest hydroxyproline (Hyp)/Pro/imino acid (Pro + Hyp) content was observed in Greenland halibut ASC (Tp is lowest), whereas the highest Ser content was observed in slime flounder ASC (Tp is highest). Using the data obtained from a previous study, a positive/negative correlation could be found between Td and Hyp/Ser, which is consistent with our previous reports. The Pro content in the present data followed the pattern of Hyp but exceeded the value predicted from Td by approximately 30 residues. These results may suggest that the collagen extracted from four flatfish in the cold area adopted decreasing imino acid content and increasing serine content for their adaptation. Full article
(This article belongs to the Section Processing and Comprehensive Utilization of Fishery Products)
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12 pages, 245 KB  
Editorial
Challenges of Fishery Biology and Fish Population Dynamics in Fisheries Management
by María de Lourdes Jiménez-Badillo
Fishes 2026, 11(5), 286; https://doi.org/10.3390/fishes11050286 - 11 May 2026
Viewed by 1046
Abstract
More than 30,000 species of bony fishes, sharks and rays have been identified worldwide [...] Full article
(This article belongs to the Special Issue The Roles of Fishery Biology and Fish Population Dynamics in Fisheries Management)
13 pages, 2268 KB  
Article
Dietary Lysophospholipids Enhance the Function of Lutein Rather than Lycopene in Pacific White Shrimp
by Chaojie Zhu, Ziling Song, Zhengwei Ye, Yanjiao Zhang, Jiahao Liu, Lindong Xiao, Chenchen Bian, Qiang Ma, Yuliang Wei, Mengqing Liang and Houguo Xu
Fishes 2026, 11(5), 285; https://doi.org/10.3390/fishes11050285 - 11 May 2026
Cited by 1 | Viewed by 811
Abstract
Our previous studies found that lipid emulsifiers can promote the absorption and deposition of liposoluble pigments in shrimp. The present study aimed to further investigate the effects of liposoluble pigments lutein (LUT) and lycopene (LYC), and their combination with lysophospholipids (LPL), on the [...] Read more.
Our previous studies found that lipid emulsifiers can promote the absorption and deposition of liposoluble pigments in shrimp. The present study aimed to further investigate the effects of liposoluble pigments lutein (LUT) and lycopene (LYC), and their combination with lysophospholipids (LPL), on the growth performance, body coloration, and lipid composition of Pacific white shrimp Penaeusvannamei. Five diets were formulated: a control diet (Control), Control + 0.02% lutein (LUT), Control + 0.02% lycopene (LYC), LUT + 0.1% LPL (LUT + LPL), and LYC + 0.1% LPL (LYC + LPL). The feeding trial lasted for 8 weeks. There were no significant differences in shrimp growth and body composition among all groups. Both LUT and LYC increased the redness (a*) and yellowness (b*) of cooked shrimp. LPL synergistically enhanced the pigmentation-improving effects of LUT but weakened those of LYC. Additionally, LYC decreased the redness and yellowness of raw shrimp. Both LUT and LYC reduced the contents of saturated fatty acids and EPA while increasing the DHA content; LPL further enhanced this modulatory effect. In conclusion, LPL acts synergistically with LUT to improve the body coloration and modify the fatty acid composition of Pacific white shrimp, but its effect with LYC is limited. Full article
(This article belongs to the Special Issue Effect of Dietary Supplementation on the Growth and Immunity of Fish and Shellfish—3rd Edition)
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14 pages, 596 KB  
Article
Dietary Glycerides of Short- and Medium-Chain Fatty Acids Modulate Intestinal Barrier and Protect Against Vibrio anguillarum in Juvenile Gilthead Sea Bream (Sparus aurata)
by Daniel Montero, Samira Sarih, Marta Carvalho, Félix Acosta, Luís Monzón-Atienza, Alvaro Gordillo, Ehab Aboelsaadat and Silvia Torrecillas
Fishes 2026, 11(5), 284; https://doi.org/10.3390/fishes11050284 - 10 May 2026
Viewed by 1137
Abstract
As aquaculture adopts more sustainable feed formulations, interest in functional feed additives has grown to help mitigate the health and performance challenges associated with low-marine-ingredient diets. This study evaluated the effects of dietary supplementation with a commercial blend of mono-, di-, and triglycerides [...] Read more.
As aquaculture adopts more sustainable feed formulations, interest in functional feed additives has grown to help mitigate the health and performance challenges associated with low-marine-ingredient diets. This study evaluated the effects of dietary supplementation with a commercial blend of mono-, di-, and triglycerides of short- and medium-chain fatty acids (SCFAs and MCFAs; BalanGUT™ AQ P, BASF) on growth, health, and disease resistance to Vibrio anguillarum in juvenile gilthead sea bream (Sparus aurata) fed practical low fishmeal and fish oil diets. Over an 8-week trial, fish were fed diets containing 0.3%, 0.5%, or 1% of a glyceride blend of SCFAs and MCFAs (BalanGUT™ AQ P) or a Control diet without functional additive supplementation. Growth performance and feed utilization were not affected by the supplementation of SCFAs/MCFAs glycerides, although non-significant trends (p > 0.05) toward improved specific growth rate (up to 12%) and reduced feed conversion ratio (up to 17%) were observed in sea bream fed supplemented diets, particularly during the 4 initial weeks and at the highest inclusion level (1%). Moderate (0.5%) and high (1%) supplementation levels of SCFAs and MCFAs significantly improved survival following Vibrio anguillarum challenge, despite no significant changes being observed in general systemic innate immune markers, such as serum lysozyme or ACH50 activities. SCFAs/MCFAs supplementation, particularly at 0.3% or 0.5%, also modulated intestinal morphology, including thinner submucosa and smaller goblet cell area in the posterior intestine, suggestive of a more homeostatic mucosa and reduced basal inflammation when feeding a low-FM/FO-based diet. These results suggest that the protective effects of this SCFAs/MCFAs glyceride blend are mediated primarily through local rather than systemic immune modulation. Overall, this study supports the use of functional SCFAs and MCFAs glyceride blends as a functional strategy to promote resilience and health in fish fed sustainable, low-marine-ingredient diets. Full article
(This article belongs to the Special Issue Advancing Aquatic Animals Nutrition: Functional Additives and Physiological Regulation)
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25 pages, 4230 KB  
Article
Optimization of Aeration Tube Configuration Considering the Efficiency of Waste Collection and Water Mixing in Aquaculture Tanks
by Can Cui, Dezhen Li, Fuzhi Zu, Dejun Feng, Xiaoyu Qu and Yi Tao
Fishes 2026, 11(5), 283; https://doi.org/10.3390/fishes11050283 - 9 May 2026
Viewed by 909
Abstract
Proper configuration of aeration tubes is crucial for improving water quality and promoting the welfare and growth of cultured organisms in Recirculating Aquaculture Systems (RASs). To investigate the effects of aeration tube shapes (arc-shaped, disc-shaped, and linear-shaped) and positions (d = r [...] Read more.
Proper configuration of aeration tubes is crucial for improving water quality and promoting the welfare and growth of cultured organisms in Recirculating Aquaculture Systems (RASs). To investigate the effects of aeration tube shapes (arc-shaped, disc-shaped, and linear-shaped) and positions (d = r, 1/2r) on performance, this study conducted experiments in a circular RAS tank. Methodologically, feed pellets were used to simulate solid waste, while ink was added to visualize the flow field, and mixing performance was quantitatively assessed using the spatial distribution uniformity. The results indicated that without aeration, the physical presence of the tubes affected waste collection but had minimal impact on water mixing. Under aeration conditions, placing tubes at d = 1/2r instead of d = r resulted in more efficient waste collection and improved water mixing. Waste collection performance was ranked as arc-shaped > disc-shaped > linear-shaped, whereas water mixing performance ranked linear-shaped > arc-shaped > disc-shaped. It is therefore recommended to place arc-shaped aeration tubes at d = 1/2r to achieve optimal overall performance. These findings provide valuable baseline insights into the selection and placement of aeration devices, offering preliminary practical guidance for aquaculture engineering. Full article
(This article belongs to the Section Sustainable Aquaculture)
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22 pages, 4992 KB  
Article
Impact of Exposure Routes of Copper Oxide Nanoparticles in the Clam Ruditapes decussatus
by Maria J. Bebianno, Mustafa Tuncsoy, Thiago L. Rocha, Tania Gomes and Taina Garcia
Fishes 2026, 11(5), 282; https://doi.org/10.3390/fishes11050282 - 9 May 2026
Cited by 1 | Viewed by 787
Abstract
The increasing production of diverse applications of engineered nanoparticles along with their potential release into the marine environment from both point and diffuse sources have become a significant concern for ocean health. Due to their unique physical properties, particularly their high surface-to-volume ratio, [...] Read more.
The increasing production of diverse applications of engineered nanoparticles along with their potential release into the marine environment from both point and diffuse sources have become a significant concern for ocean health. Due to their unique physical properties, particularly their high surface-to-volume ratio, these nanoparticles can exhibit enhanced bioavailability and toxicity to marine biota. Copper oxide nanoparticles (CuO NPs) are especially prevalent due to their wide range of commercial applications. In the aquatic environment, these nanoparticles typically become part of colloidal fraction and are subjected to physicochemical transformations, leading to the formation of aggregates that eventually sink and deposit onto the bottom substrate. Therefore, sediments, in addition to the water column, act as the primary route of exposure to benthic organisms. The clam Rudipates decussatus is a marine suspension-feeder of great ecological and economic importance in Europe. Ruditapes decussatus were exposed to CuO NPs (10 μg L−1) or an equivalent concentration of ionic copper (Cu2+) in both water and water/sediments matrices for 15 days to compare the toxicological impact of different exposure routes. Copper accumulation was monitored in both gills and digestive gland, alongside various biomarkers of susceptibility, exposure, and damage. The results revealed distinct uptake patterns that were dependent on the exposure routes, the chemical form of the metal and the specific tissue responses. Highlighting the complex impact of these contaminants on marine biodiversity. Full article
(This article belongs to the Section Environment and Climate Change)
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23 pages, 17215 KB  
Article
Comparative In Vitro Bioactivity of Traditional Aqueous and Alcoholic Preparations of Arnica (Chiliadenus glutinosus): Effects on Marine Fish Pathogens, PLHC1 Cells and Gilthead Seabream (Sparus aurata) Leucocytes
by Jose Carlos Campos-Sánchez, Francisco A. Guardiola and María Ángeles Esteban
Fishes 2026, 11(5), 281; https://doi.org/10.3390/fishes11050281 - 9 May 2026
Viewed by 559
Abstract
Arnica (Chiliadenus glutinosus (L.) Fourr.) is an endemic plant widely used in Spanish traditional medicine as infusions and alcoholic macerates for different ailments. Despite this use, information about the biological activity of these preparations in fish-related models is scarce. In the present [...] Read more.
Arnica (Chiliadenus glutinosus (L.) Fourr.) is an endemic plant widely used in Spanish traditional medicine as infusions and alcoholic macerates for different ailments. Despite this use, information about the biological activity of these preparations in fish-related models is scarce. In the present study, the arnica extract bioactivity assay evaluated aqueous, ethanolic, and methanolic extracts at different doses (0, 0.001, 0.01, 0.125, 0.25, 0.5, and 1 mg mL−1) to compare their antioxidant activity, effects on four marine fish pathogens (Vibrio harveyi, Vibrio anguillarum, Photobacterium damselae and Tenacibaculum maritimum), cytotoxicity on the PLHC1 tumour cell line, and their impact on immunological parameters in head-kidney leucocytes (HKLs) of gilthead seabream (Sparus aurata). All extracts showed dose-dependent antioxidant activity, while bactericidal effects depended on the solvent and were mainly observed at the highest concentrations. Ethanolic and methanolic extracts displayed clear cytotoxicity, whereas the aqueous extract showed lower toxicity and was selected for further evaluation. In the carrageenan stimulation assay, selected concentrations (0, 0.25, and 0.5 mg mL−1) of the aqueous extract were tested in leucocytes stimulated with λ-carrageenan (0 and 1000 µg mL−1), and respiratory burst and phagocytic activity, cell morphology, and gene expression were analysed. The aqueous extract reduced respiratory burst and phagocytic capacity in activated leucocytes and was associated with morphological signs of cell activation. It also downregulated crel and casp9 expression. These results provide a comparative view of the in vitro bioactivity of different traditional preparations of arnica and show that their biological effects strongly depend on the solvent used and the concentration tested, providing initial experimental information on their cellular effects in fish. Full article
(This article belongs to the Section Physiology and Biochemistry)
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23 pages, 5389 KB  
Article
An Edge-Ready Lightweight Computer Vision Framework for On-Site Fish Disease Detection in Aquaculture Management
by Jiawen Li, Weidong Zhang, Shengzhao Xiao, Xuanzhong Chen, Yuesheng Huang, Jujian Lv, Kaihan Lin, Xianglei Hu, Xianxian Zeng and Rongjun Chen
Fishes 2026, 11(5), 280; https://doi.org/10.3390/fishes11050280 - 9 May 2026
Cited by 2 | Viewed by 1041
Abstract
Efficient detection of fish diseases is essential for intelligent health monitoring and timely intervention in aquaculture. However, current computer vision models remain computationally intensive, hindering their deployment on resource-constrained edge devices in aquaculture applications. To this end, this study developed a lightweight detection [...] Read more.
Efficient detection of fish diseases is essential for intelligent health monitoring and timely intervention in aquaculture. However, current computer vision models remain computationally intensive, hindering their deployment on resource-constrained edge devices in aquaculture applications. To this end, this study developed a lightweight detection framework based on an improved You Only Look Once (YOLO), aiming to achieve a favorable balance between detection accuracy and on-site inference efficiency. First, a Dual-Branch Feature-Preserving Downsampling (DFPD) module was proposed to enhance the extraction of valuable disease-related cues with minimal computational overhead. Subsequently, structured pruning was applied to compress the optimized baseline model. Four pruning techniques, including Slim, GroupTaylor, Layer-Adaptive Magnitude-Based Pruning (LAMP), and L1-based, were evaluated under the same conditions. The enhanced baseline model improved precision from 0.864 to 0.908 and mAP@0.5:0.95 from 0.613 to 0.632, while already reducing the Number of Parameters (Params) and Giga Floating-point Operations Per Second (GFLOPs) compared with the original YOLOv8n. Among the pruning techniques, L1-based produced the best overall trade-off, yielding a final model that maintained a F1-score of 0.860 while reducing Params and GFLOPs by 54.7% and 49.4%, respectively, relative to the original detector. Ablation studies further revealed that a moderate FLOPs reduction of approximately 41% to 47% was optimal for preserving diagnostic performance while enhancing compactness. Edge deployment tests on an RK3588S device verified the framework’s practical inference speed advantage. Therefore, this study offers a deployment-friendly computer vision solution for on-site fish disease detection in aquaculture management, particularly suited to real-world scenarios with limited computational resources. Full article
(This article belongs to the Special Issue Computer Vision Applications for Fisheries and Aquaculture)
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23 pages, 1878 KB  
Article
Impact of Solubilized Mannan Oligosaccharide Supplementation on Growth Performance, Digestive Health, Stress Resistance, and Economic Efficiency in Pacific White Shrimp (Penaeus vannamei) Raised in an Intensive Synbiotic System
by Danielle Alves da Silva, Flávia Abreu Everton, Gisely Karla de Almeida Costa, Suzianny Maria Bezerra Cabral da Silva, Fernando Leandro dos Santos, Rodrigo Antônio Ponce de Leon Ferreira de Carvalho, Giovani Sampaio Gonçalves, João Fernando Albers Koch and Luis Otavio Brito
Fishes 2026, 11(5), 279; https://doi.org/10.3390/fishes11050279 - 9 May 2026
Cited by 2 | Viewed by 1124
Abstract
The present study investigated how dietary inclusion of solubilized mannan oligosaccharide (MOS) influences growth performance, digestive health, stress resilience, and production profitability in Pacific white shrimp (Penaeus vannamei) reared under intensive synbiotic conditions. Juveniles averaging 3.00 ± 0.04 g were stocked [...] Read more.
The present study investigated how dietary inclusion of solubilized mannan oligosaccharide (MOS) influences growth performance, digestive health, stress resilience, and production profitability in Pacific white shrimp (Penaeus vannamei) reared under intensive synbiotic conditions. Juveniles averaging 3.00 ± 0.04 g were stocked at 100 shrimp m−2 and fed experimental diets containing 0 (control), 1.0, 2.0, or 4.0 g kg−1 MOS for 60 days. Shrimp receiving 1.0 g kg−1 MOS showed higher growth rate, improved feed conversion, and greater final body weight than the control (p < 0.05), indicating enhanced feed utilization efficiency and better overall performance. Gut morphology improved in MOS-fed treatments, with increased mucosal fold height and enterocyte height, suggesting increased nutrient absorption and improved gut functionality. Gut presumptive total count remained relatively stable among treatments, although Bacillus counts tended to increase with solubilized MOS supplementation. Under ammonia and nitrite stress, supplemented groups showed higher survival and reduced gill damage, indicating improved physiological tolerance and health status. Economic analysis demonstrated that 1.0 and 2.0 g kg−1 MOS achieved the best cost–benefit ratios under intensive conditions. Overall, moderate MOS supplementation enhanced growth, gut morphology, stress resistance, and economic efficiency. Polynomial regression analysis, based on the four dietary inclusion levels evaluated, suggested that approximately 1.5 g kg−1 MOS may represent an estimated optimal inclusion level. Full article
(This article belongs to the Special Issue Sustainable Aquaculture of Crustaceans)
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