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Separations, Volume 12, Issue 6

2025 June - 33 articles

Cover Story: The development of chromatographic processes to purify biopharmaceuticals requires tremendous experimental effort. The state-of-the-art approach, Design of Experiments, enables a systematic procedure limited to a design space described by a statistical, interpolating model; however, the experimental burden remains high. Process development would benefit from extrapolating models to reduce this burden and enable deeper process understanding and knowledge transfer. Nevertheless, such models currently lack predictive power. In an academic–industrial collaboration, we present a new biophysics-based adsorption model for developing ion-exchange chromatography processes for pharmaceuticals at a preparative scale. This model achieves high predictive power across low and high antibody load densities and even captures charge variant behavior. View this paper
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Articles (33)

  • Article
  • Open Access
1,154 Views
11 Pages

Separations of Strategic Metals from Spent Electronic Waste Using “Green Methods”

  • Urszula Domańska,
  • Anna Wiśniewska and
  • Zbigniew Dąbrowski

Next-generation recycling technologies must be urgently innovated to tackle huge volumes of spent batteries, photovoltaic panels or printed circuit boards (WPCBs). Current e-waste recycling industrial technology is dominated by traditional recycling...

  • Article
  • Open Access
1,222 Views
10 Pages

Simulation of Helical-Baffle Inlet Structure Cyclone Separator

  • Guohua Li,
  • Jie Gong,
  • Zijuan Wang and
  • Ran Liu

In developing spacecraft dust environment testing equipment, cyclone separators serve as critical particulate separation devices. To optimize cyclone performance, this study investigates the impact of inlet configurations on internal flow fields. We...

  • Article
  • Open Access
1,118 Views
14 Pages

The Quality by Design (QbD) concept has been widely applied to the optimization of traditional Chinese medicine production processes recently. This work focused on optimizing the critical purification process of Scutellaria baicalensis extract used i...

  • Article
  • Open Access
1 Citations
1,007 Views
23 Pages

Separation of Bioactive Compounds from Pfaffia glomerata: Drying, Green Extraction, and Physicochemical Properties

  • Marcela Moreira Terhaag,
  • Ana Catarina Mosquera dos Santos,
  • Daniel Gonzaga de Lima,
  • Otavio Akira Sakai,
  • Giselle Giovanna do Couto de Oliveira,
  • Cristiane Mengue Feniman Moritz,
  • Bogdan Demczuk Junior,
  • Jorcilene dos Santos Silva,
  • Suelen Pereira Ruiz and
  • Erica Marusa Pergo Coelho
  • + 2 authors

Leaves (LV), stems (STs), and inflorescences (IFs) of Pfaffia glomerata are usually discarded despite containing various bioactive compounds, especially β-ecdysone saponin. The objective was to optimize by desirability (DI) the ultrasound-assist...

  • Article
  • Open Access
1,115 Views
13 Pages

The Selective Extraction of Natural Sesquiterpenic Acids in Complex Matrices: A Novel Strategy for Isolating Zizanoic Acid in Vetiver Essential Oil

  • Ian Gardel Carvalho Barcellos-Silva,
  • Ananda da Silva Antonio,
  • Mateus Curty Cariello da Silva,
  • Fernanda de Melo Regazio Cariello,
  • Fernando Hallwass,
  • Monica Costa Padilha and
  • Valdir Florencio Veiga-Junior

Essential oils are complex mixtures of apolar components, mainly phenylpropanoids, monoterpenes, and sesquiterpenes. Vetiver (Vetiveria zizanioides (L.) Nash) is a non-endemic grass in several tropical regions, widely used for slope stabilization and...

  • Review
  • Open Access
1 Citations
2,458 Views
25 Pages

Membrane technologies are used in the production of potable water and the treatment of wastewater in the military forces, providing the highest level of contaminant removal at an energy-efficient cost. This review examines the integration and applica...

  • Article
  • Open Access
1,228 Views
20 Pages

This study focuses on optimizing two tandem columns to separate ethylbenzene and styrene. A steady-state model is developed to minimize total energy consumption (TEC) and total annualized cost (TAC) by optimizing the reflux flow rates. An integrated...

  • Review
  • Open Access
2 Citations
4,987 Views
27 Pages

Current Perspectives on the Extraction, Isolation, and Identification of Fats and Fatty Acids Using Conventional and Green Methods

  • Ytaiara Lima-Pereira,
  • Esther Maria Oliveira de Souza,
  • David Silva dos Reis,
  • Ian Gardel Carvalho Barcellos-Silva,
  • Karine Sayuri Lima Miki,
  • Valdir F. Veiga-Júnior and
  • Barbara Elisabeth Teixeira-Costa

The global demand for oils and lipids, particularly those derived from vegetable sources with high polyunsaturated fatty acid content, has posed significant challenges for the food industry. This trend is largely driven by growing consumer awareness...

  • Article
  • Open Access
2 Citations
816 Views
14 Pages

Multivariate Analysis of UPLC-MS/MS Metabolomic Profiles in Four Hiraea Species (Malpighiaceae)

  • Jaqueline Munise Guimarães da Silva,
  • Rafael Felipe de Almeida and
  • Maria Luiza Zeraik

The presence of bioactive compounds is reported in several Malpighiaceae species. However, little metabolomic information is documented in the genus Hiraea (Malpighiaceae). Thus, the objective was to identify secondary metabolites in the leaves of Hi...

  • Article
  • Open Access
2 Citations
1,055 Views
16 Pages

Comparison of Extraction Techniques for Wide Screening of 230 Pesticides in Water

  • Caterina Cacciatori,
  • Jackie Myers,
  • Giulio Mariani,
  • Hung Vu,
  • Bernd Manfred Gawlik and
  • Vincent Pettigrove

In this study, weekly grab samples extracted by solid-phase extraction (SPE) and stir bar sorptive extraction (SBSE) were compared for the analysis of 230 pesticides in surface waters. Samples were collected from three different locations around Melb...

  • Article
  • Open Access
1,041 Views
22 Pages

This study presents a cost-effective and feasible technique for the deep denitrification of wastewater, based on sulfur autotrophic denitrification mediated by polysulfides (Sn2). Various polysulfides were used as electron donors in an aerobic...

  • Article
  • Open Access
2 Citations
1,118 Views
21 Pages

Green Extraction Combined with Chemometric Approach: Profiling Phytochemicals and Antioxidant Properties of Ten Species of the Lamiaceae Family

  • Branislava Teofilović,
  • Emilia Gligorić,
  • Martina Ninić,
  • Saša Vukmirović,
  • Žarko Gagić,
  • Nebojša Mandić-Kovačević,
  • Biljana Tubić,
  • Đorđe Đukanović and
  • Nevena Grujić-Letić

The pharmacological potential of Lamiaceae plants is primarily linked to their high content of phenolic acids and flavonoids, known for strong antioxidant properties. This study investigated the antioxidant activity of ten widely used Lamiaceae herbs...

  • Review
  • Open Access
3 Citations
4,354 Views
26 Pages

Antimony Recovery from Industrial Residues—Emphasis on Leaching: A Review

  • Marinela Panayotova,
  • Serhii Pysmennyi and
  • Vladko Panayotov

Antimony (Sb) is a metalloid widely used in different areas—from the cutting-edge renewable energy technologies to “classical” lead acid batteries. Its availability in primary sources is limited, and these sources are geographically...

  • Article
  • Open Access
1 Citations
1,169 Views
13 Pages

The unregulated consumption of corticosteroids causes significant adverse effects on human health. Therefore, it is important to develop methodologies that allow their analysis in pharmaceutical matrices with competitive analysis times and costs. The...

  • Article
  • Open Access
754 Views
11 Pages

Hydrothermal conversion is an effective strategy to transform heavy metals in electroplating sludge into catalytic materials and use them to treat electroplating wastewater. This study presents a one-step hydrothermal method for converting Sn-bearing...

  • Article
  • Open Access
1,150 Views
20 Pages

The efficient separation of light hydrocarbons, particularly alkanes from their isomers (C5–C6), represents a significant and energy-intensive challenge for the petrochemical industry. Metal-Organic Frameworks (MOFs) offer promising solutions d...

  • Article
  • Open Access
2 Citations
952 Views
14 Pages

Physicochemical and Phytochemical Determinations of Greek “Kollitsida’’ (Arctium lappa L.) from Different Regions and Evaluation of Its Antimicrobial Activity

  • Dimitrios G. Lazaridis,
  • Sokratis D. Giannoulis,
  • Maria Simoni,
  • Vassilios K. Karabagias,
  • Nikolaos D. Andritsos,
  • Vasileios Triantafyllidis and
  • Ioannis K. Karabagias

The present study aimed to investigate Greek Kollitsida root samples from different geographical regions based on physicochemical and antibacterial analyses. For this purpose, samples were treated with different solvents, deionized water (solvent A)...

  • Article
  • Open Access
2 Citations
1,825 Views
19 Pages

The hydrocyclone generally exhibits limited separation efficiency and classification sharpness. As the discharge channel for fine particles, the vortex finder plays a critical role in influencing the classification performance through its structural...

  • Review
  • Open Access
8 Citations
4,611 Views
19 Pages

As sustainability gains prominence, the circular economy has encouraged the valorization of agri-food by-products, which are rich in phenolic compounds known for their antioxidant and anti-inflammatory properties. Conventional extraction methods comm...

  • Article
  • Open Access
3 Citations
2,536 Views
20 Pages

Effective Liquid–Liquid Extraction for the Recovery of Grape Pomace Polyphenols from Natural Deep Eutectic Solvents (NaDES)

  • Alessandro Frontini,
  • Giulio Tarentini,
  • Carmine Negro,
  • Andrea Luvisi,
  • Massimiliano Apollonio and
  • Luigi De Bellis

Natural deep eutectic solvents (NaDESs) are emerging solvents for their yield when used for extraction of different molecules, including polyphenols. NaDESs are a cutting-edge technology that offers numerous advantages, including cheap cost, safety,...

  • Article
  • Open Access
1,193 Views
23 Pages

The development of ion exchange chromatography to polish biopharmaceuticals requires extensive experimental benchmarking. As part of the Design of Experiments (DoE), statistical models increased efficiency somewhat and are still state of the art; how...

  • Article
  • Open Access
1,304 Views
18 Pages

Insights into the Adsorptive Separation of Ethylene/Ethane in LTA-Type Zeolites

  • Xiaohui Zhao,
  • Shixue Zhou,
  • Magdy Abdelghany Elsayed,
  • Zhongyuan Chen,
  • Chunhui Jiang,
  • Yongli Hu and
  • Gumawa Windu Manggada

Understanding the competitive adsorption mechanism is essential for the development of adsorptive separation of ethylene (C2H4) and ethane (C2H6). In this work, density functional theory calculations and molecular dynamics simulations were employed t...

  • Article
  • Open Access
1 Citations
1,245 Views
15 Pages

Essential oils (EOs), which are complex mixtures of plant-derived volatile compounds, have been utilized for centuries in the medical, food, and pharmaceutical industries because of their diverse biological properties. In recent years, there has been...

  • Review
  • Open Access
2,741 Views
16 Pages

Volatile C2–C5 olefins are important bulk chemicals in the polymer industry. Traditionally, C2–C5 olefins are produced from cracked petroleum resources using an energy-consuming and hazardous distillation method. Currently, volatile olefi...

  • Article
  • Open Access
813 Views
11 Pages

Analysis on the Main Components of Selenium-Enriched Premna microphylla Leaves and Processed Tofu

  • Jianan Wang,
  • Chunli Chen,
  • Fangjie Mou,
  • Bin Wang and
  • Jingzhou Dong

Premna microphylla is a medicinal plant species distributed in Southeast Asia and China. P. microphylla leaves have been widely used for processing edible gels called Chai tofu, which have many medicinal values, such as clearing heat and detoxifying....

  • Article
  • Open Access
1,131 Views
13 Pages

This study aims to develop a simultaneous analytical method for detecting 19 pesticides, including 4,4′-DDD, in fishery products using gas chromatography with micro-electron capture detection (GC-μECD) and gas chromatography–tandem mas...

  • Article
  • Open Access
1,070 Views
14 Pages

Herein, a simple and effective analytical method was developed to monitor etoricoxib concentrations in human urine samples. Etoricoxib is a nonsteroidal anti-inflammatory drug for pain and inflammation relief in conditions such as osteoarthritis and...

  • Article
  • Open Access
3 Citations
1,237 Views
15 Pages

Multi-Template Molecularly Imprinted Polymers Coupled with a Solid-Phase Extraction System in the Selective Determination of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) in Environmental Water Samples

  • David Aurelio-Soria,
  • Giaan A. Alvarez-Romero,
  • Maria E. Paez-Hernandez,
  • I. Perez-Silva,
  • Miriam Franco-Guzman,
  • Gabriela Islas and
  • Israel S. Ibarra

A simple, fast, and low-cost pre-concentration methodology based on the application of multi-template molecularly imprinted polymers (mt-MIP) in a solid-phase extraction system coupled with capillary electrophoresis was developed for the determinatio...

  • Article
  • Open Access
1,018 Views
18 Pages

An Optimized Pathway for Nitrate Removal from Aqueous Solution by Environmentally Friendly Calabash Gourd Shell Adsorbent Based on Experimental Design Methodology

  • Goran S. Nikolić,
  • Nataša Simonović,
  • Miloš Durmišević,
  • Nada Nikolić,
  • Dragana Marković Nikolić,
  • Milena Nikolić,
  • Grozdanka Bogdanović and
  • Aleksandar Bojić

The aim of this research is to optimize the process parameters for nitrate adsorption from aqueous solutions using ammonium modified calabash gourd shell (CGS). Two types of experimental design (DoE) methodology were implemented in the optimization....

  • Article
  • Open Access
1 Citations
1,048 Views
16 Pages

The rapid isolation of target constituents from natural products poses a significant challenge and is a key focus in current research. The Hosta plantaginea flower (HPF), a traditional Chinese medicinal herb, is primarily used to treat inflammatory d...

  • Article
  • Open Access
777 Views
21 Pages

Influence of Micron Roughness on Droplet Adhesion and Detachment Behavior with Coal Surfaces

  • Siheng Hou,
  • Peng Xiong,
  • Xianshu Dong,
  • Hengfei Chen,
  • Shuhuan Li and
  • Yujin Sun

Surface roughness directly affects the interaction between mineral surfaces and water, as well as the adhesion of particles to bubbles during mineral flotation processes. Currently, there is a significant amount of research on the relationship betwee...

  • Article
  • Open Access
824 Views
14 Pages

Mixed-Mode Chromatography: Studies on Hybrid Retention Mechanisms of Some Antihypertensive Drugs

  • Irinel Adriana Badea,
  • Andrei Mihăilă,
  • Dana Elena Popa,
  • Anca Monica Tencaliec and
  • Mihaela Buleandră

The antihypertensive drugs indapamide, atenolol, metoprolol, propranolol and bisoprolol were considered in this research. Because they have structures that are affected by pH, developing a chromatographic method was challenging. Based on the speciati...

  • Article
  • Open Access
1 Citations
2,147 Views
17 Pages

Mogroside V crude extract from Siraitia grosvenorii has many pharmacological effects, such as anti-diabetes, antioxidant, etc. It is being used as a kind of natural sweetener in more and more countries. The improvement of Mogroside V purity can great...

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Separations - ISSN 2297-8739