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Separations, Volume 12, Issue 6

June 2025 - 33 articles

Cover Story: The development of chromatographic processes to purify biopharmaceuticals requires tremendous experimental effort. The state-of-the-art approach, Design of Experiments, enables a systematic procedure limited to a design space described by a statistical, interpolating model; however, the experimental burden remains high. Process development would benefit from extrapolating models to reduce this burden and enable deeper process understanding and knowledge transfer. Nevertheless, such models currently lack predictive power. In an academic–industrial collaboration, we present a new biophysics-based adsorption model for developing ion-exchange chromatography processes for pharmaceuticals at a preparative scale. This model achieves high predictive power across low and high antibody load densities and even captures charge variant behavior. View this paper
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Articles (33)

  • Article
  • Open Access
979 Views
11 Pages

Separations of Strategic Metals from Spent Electronic Waste Using “Green Methods”

  • Urszula Domańska,
  • Anna Wiśniewska and
  • Zbigniew Dąbrowski

Next-generation recycling technologies must be urgently innovated to tackle huge volumes of spent batteries, photovoltaic panels or printed circuit boards (WPCBs). Current e-waste recycling industrial technology is dominated by traditional recycling...

  • Article
  • Open Access
955 Views
10 Pages

Simulation of Helical-Baffle Inlet Structure Cyclone Separator

  • Guohua Li,
  • Jie Gong,
  • Zijuan Wang and
  • Ran Liu

In developing spacecraft dust environment testing equipment, cyclone separators serve as critical particulate separation devices. To optimize cyclone performance, this study investigates the impact of inlet configurations on internal flow fields. We...

  • Article
  • Open Access
1,006 Views
14 Pages

The Quality by Design (QbD) concept has been widely applied to the optimization of traditional Chinese medicine production processes recently. This work focused on optimizing the critical purification process of Scutellaria baicalensis extract used i...

  • Article
  • Open Access
1 Citations
872 Views
23 Pages

Separation of Bioactive Compounds from Pfaffia glomerata: Drying, Green Extraction, and Physicochemical Properties

  • Marcela Moreira Terhaag,
  • Ana Catarina Mosquera dos Santos,
  • Daniel Gonzaga de Lima,
  • Otavio Akira Sakai,
  • Giselle Giovanna do Couto de Oliveira,
  • Cristiane Mengue Feniman Moritz,
  • Bogdan Demczuk Junior,
  • Jorcilene dos Santos Silva,
  • Suelen Pereira Ruiz and
  • Maria Graciela Iecher Faria
  • + 2 authors

Leaves (LV), stems (STs), and inflorescences (IFs) of Pfaffia glomerata are usually discarded despite containing various bioactive compounds, especially β-ecdysone saponin. The objective was to optimize by desirability (DI) the ultrasound-assist...

  • Article
  • Open Access
899 Views
13 Pages

The Selective Extraction of Natural Sesquiterpenic Acids in Complex Matrices: A Novel Strategy for Isolating Zizanoic Acid in Vetiver Essential Oil

  • Ian Gardel Carvalho Barcellos-Silva,
  • Ananda da Silva Antonio,
  • Mateus Curty Cariello da Silva,
  • Fernanda de Melo Regazio Cariello,
  • Fernando Hallwass,
  • Monica Costa Padilha and
  • Valdir Florencio Veiga-Junior

Essential oils are complex mixtures of apolar components, mainly phenylpropanoids, monoterpenes, and sesquiterpenes. Vetiver (Vetiveria zizanioides (L.) Nash) is a non-endemic grass in several tropical regions, widely used for slope stabilization and...

  • Review
  • Open Access
1 Citations
1,832 Views
25 Pages

Membrane technologies are used in the production of potable water and the treatment of wastewater in the military forces, providing the highest level of contaminant removal at an energy-efficient cost. This review examines the integration and applica...

  • Article
  • Open Access
1,007 Views
20 Pages

This study focuses on optimizing two tandem columns to separate ethylbenzene and styrene. A steady-state model is developed to minimize total energy consumption (TEC) and total annualized cost (TAC) by optimizing the reflux flow rates. An integrated...

  • Review
  • Open Access
1 Citations
4,145 Views
27 Pages

Current Perspectives on the Extraction, Isolation, and Identification of Fats and Fatty Acids Using Conventional and Green Methods

  • Ytaiara Lima-Pereira,
  • Esther Maria Oliveira de Souza,
  • David Silva dos Reis,
  • Ian Gardel Carvalho Barcellos-Silva,
  • Karine Sayuri Lima Miki,
  • Valdir F. Veiga-Júnior and
  • Barbara Elisabeth Teixeira-Costa

The global demand for oils and lipids, particularly those derived from vegetable sources with high polyunsaturated fatty acid content, has posed significant challenges for the food industry. This trend is largely driven by growing consumer awareness...

  • Article
  • Open Access
1 Citations
727 Views
14 Pages

Multivariate Analysis of UPLC-MS/MS Metabolomic Profiles in Four Hiraea Species (Malpighiaceae)

  • Jaqueline Munise Guimarães da Silva,
  • Rafael Felipe de Almeida and
  • Maria Luiza Zeraik

The presence of bioactive compounds is reported in several Malpighiaceae species. However, little metabolomic information is documented in the genus Hiraea (Malpighiaceae). Thus, the objective was to identify secondary metabolites in the leaves of Hi...

  • Article
  • Open Access
1 Citations
913 Views
16 Pages

Comparison of Extraction Techniques for Wide Screening of 230 Pesticides in Water

  • Caterina Cacciatori,
  • Jackie Myers,
  • Giulio Mariani,
  • Hung Vu,
  • Bernd Manfred Gawlik and
  • Vincent Pettigrove

In this study, weekly grab samples extracted by solid-phase extraction (SPE) and stir bar sorptive extraction (SBSE) were compared for the analysis of 230 pesticides in surface waters. Samples were collected from three different locations around Melb...

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Separations - ISSN 2297-8739