Green Extraction of Natural Products for Application in Pharmaceuticals, Foods, Cosmetics and Agriculture

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Analysis of Natural Products and Pharmaceuticals".

Deadline for manuscript submissions: 10 October 2025 | Viewed by 506

Special Issue Editors


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Guest Editor
Instituto Politécnico Nacional, Oaxaca 71233, Mexico
Interests: green chemistry-assisted extraction methods; natural products; isolation of bioactive molecules with medicinal use; essential oils; phenolic compounds; compound identification; UPLC-ESI- qTOF- MS/MS; GC-MS/MS TQ

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Guest Editor
Área Académica de Ingeniería Agroindustrial e Ingeniería en Alimentos, Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad km. 1, Ex-Hacienda de Aquetzalpa, Tulancingo 43600, Mexico
Interests: antioxidants extraction; chemical composition; intestinal bioaccesibility of bioactive compounds

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Guest Editor
1. Laboratoire de Chimie Agroindustrielle, Institut National Polytechnique, INRAe-Toulouse, Toulouse, France
2. Département Génie Biologique, IUT, Université de Toulouse, Toulouse, France
Interests: bioactive accumulation and extraction; plant physiology; genetics; abiotic stress; lipids; essential oils; biofertilizers; cereals; oilseed crops; viticulture
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Special Issue Information

Dear Colleagues,

Due to the increasing demand for naturalness in our society, researchers and the industry are now aiming to find active molecules and develop methods for the extraction and application of these molecules that meet the demand for naturalness. The extraction of bioactive molecules using green chemistry ensures the environmental protection of the natural products employed in pharmaceuticals, food, and cosmetics. The energies generated via the use of ultrasonics, microwaves, thermomagnetic induction, the subcritical or supercritical conditions of solvents such as water, CO2 and N2, and SPME (solid-phase microextraction) are an alternative to conventional methods. Traditional knowledge regarding the use of plant species represents a starting point for the discovery of new compounds that could have a significant impact on the world. Gas and liquid chromatography techniques, mass spectrometry, time-of-flight analyzers, and other related techniques enable the efficiency of extraction processes to be measured through yield, purity, and the selective isolation of compounds. The optimization of these methods could reduce the number of experiments performed and the processing time, among other variables.

In this Special Issue, we welcome submissions that explore new green extraction methods, strategies that enhance the yield of compounds, and their effects on the development of novel pharmaceuticals, food products, cosmetics and agricultural applications.

Prof. Dr. Luicita Lagunez-Rivera
Prof. Dr. Esther Ramirez Moreno
Dr. Othmane Merah
Guest Editors

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Keywords

  • plant species sources
  • orchids
  • primary metabolites
  • secondary metabolites
  • essential oils
  • fragrances
  • antioxidant activity
  • pharmaceutical application
  • nutraceutical application
  • molecular nutrition
  • intestinal bioaccessibility
  • agricultural uses

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Published Papers (1 paper)

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Research

16 pages, 4649 KiB  
Article
Rapid Two-Step Isolation of Kaempferol from the Hosta plantaginea Flower and Its Anti-Inflammatory Mechanism: Evidence from Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and Experimental Validation
by Yating Yang, Bowei Xia, Huan Ouyang, Junyu Guo, Qingya Hu, Li Yang and Junwei He
Separations 2025, 12(6), 138; https://doi.org/10.3390/separations12060138 - 23 May 2025
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Abstract
The rapid isolation of target constituents from natural products poses a significant challenge and is a key focus in current research. The Hosta plantaginea flower (HPF), a traditional Chinese medicinal herb, is primarily used to treat inflammatory diseases, with kaempferol as one of [...] Read more.
The rapid isolation of target constituents from natural products poses a significant challenge and is a key focus in current research. The Hosta plantaginea flower (HPF), a traditional Chinese medicinal herb, is primarily used to treat inflammatory diseases, with kaempferol as one of its major bioactive constituents. In this study, macroporous adsorption resin was used to purify total flavonoids (TF) from the HPFs. The 50% ethanol–water elution fraction of the TF was then recrystallized to yield kaempferol with a purity of 99.44%. Network pharmacology analysis identified 61 potential kaempferol-inflammation targets, which were linked to the PI3K-Akt and TNF signaling pathways. Molecular docking and molecular dynamics simulations revealed the stability and binding of kaempferol to PI3K, Akt, and TNF-α proteins. The analysis metrics included binding ability, the root mean square deviation (RMSD), radius of gyration, free energy landscape, solvent-accessible surface area, hydrogen bond count, RMS fluctuation, free binding energy, amino acid residue free energy decomposition, and principal component analysis. The anti-inflammatory mechanism of kaempferol was further validated in an LPS-induced RAW264.7 cell model, where it was shown to inhibit the PI3K-Akt and TNF-α signaling pathways. This study provides new insights into the anti-inflammatory mechanism of kaempferol and presents novel strategies for the rapid isolation of target constituents from natural products. Full article
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