Table of Contents
Nanomaterials, Volume 9, Issue 11 (November 2019) – 139 articles
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Cover Story (view full-size image) The Surface-Enhanced Raman Spectroscopy (SERS) immunoassay consists in bioconjugates of gold [...] Read more. The Surface-Enhanced Raman Spectroscopy (SERS) immunoassay consists in bioconjugates of gold nanostars (AuNSs) functionalised with 4-mercaptobenzoic acid as a Raman reporter, and polyclonal anti-horseradish peroxidase antibodies as the detection moiety. Agarose gel electrophoresis is instrumental in determining an effective functionalisation of the AuNSs by the Raman reporter, further conjugation with antibodies and to mimic the final SERS sandwich immunoassay. The optimised SERS-active bioconjugates allowed effective SERS detection down to 0.01 nM of bioconjugates, corresponding to an antigen peroxidase activity of 50 µU. Our results are a proof-of-concept for a SERS-based immunoassay with easy adaptation to alternative antibody–antigen pairs. View this paper.