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Appl. Sci., Volume 10, Issue 2 (January-2 2020) – 305 articles
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Cover Story (view full-size image) Haematococcus pluvialis accumulates astaxanthin, which is a high-value antioxidant, during the red [...] Read more. Haematococcus pluvialis accumulates astaxanthin, which is a high-value antioxidant, during the red cyst stage of its lifecycle. Development of a rigid cell wall in the cysts hinders the recovery of astaxanthin. This article investigates morphological changes and cell disruption of mature Haematococcus pluvialis cysts while using high-pressure homogenization for astaxanthin extraction. Mechanical treatment resulted in 91% of cell disruption and a proportional increase in astaxanthin recovery. This study recommends a synergistic combination of rapid instrumental particle-volume-distribution analysis and microscope-based morphologic phenotyping for the development of practical biorefinery processes that co-produce various biological products, including lipids, proteins, carbohydrates, and astaxanthin. View this paper.