This paper presents an innovative and low-cost approach to the dispensing of multiple liquids on a microfluidic chip with the aim of dispensing liquids in a controlled sequence. The project focused on the design and development of a microfluidic liqu...
Electro-wetting-on-dielectric (EWOD) enables the manipulation of droplets on a two-dimensional surface, which provides a versatile technique for digital microfluidics at a micro- or nano-scale. However, the deficiency of the dispensing precision has...
As an efficient approach to risk management in agriculture, the elimination of losses due to plant diseases and insect pests is one of the most important and urgent technological challenges for improving the crop yield. Therefore, we have developed a...
Living cell microarrays in microfluidic chips allow the non-invasive multiplexed molecular analysis of single cells. Here, we developed a simple and affordable perfusion microfluidic chip containing a living yeast cell array composed of a population...
Microfluidic systems have been regarded as a potential platform for high-throughput screening technology in drug discovery due to their low sample consumption, high integration, and easy operation. The handling of small-volume liquid is an essential...
Digital microfluidic systems based on electrowetting-on-dielectric technology, particularly valuable in producing and manipulating microdroplets steadily and consistently, have experienced notable advancements in recent years. In this paper, experime...
We introduce a novel approach for highly parallel droplet dispensing with precise control over the droplet parameters such as droplet volume, droplet velocity, etc. This approach facilitates the fabrication of homogeneous and precise thin layers with...
Advances in micro and nano fabrication technologies have enabled fabrication of smaller and more sensitive devices for applications not only in solid-state physics but also in medicine and biology. The demand for devices that can precisely transport...
The reliable non-contact dispensing of droplets in the pico- to microliter range is a challenging task. The dispensed drop volume depends on various factors such as the rheological properties of the liquids, the actuation parameters, the geometry of...
In breast cancer research, immunomagnetic enrichment of circulating tumor cells (CTCs) from body fluids has impressively evolved over the last decades. However, there is growing interest in further singularizing these pre-enriched rare cells to decre...
A simple microfluidic control method that uses a piezoelectric dispenser head is developed to fabricate microdots. A glycerol mixture was used as the test fluid to simulate conductive metallic solutions. The orifice diameter of the dispenser was 50 μ...
In this work, the feasibility of a novel direction for microfluidics is studied by demonstrating a set of new methods to fabricate microfluidic systems. Similarly to microfluidic paper-based analytical devices, porous materials are being used. Howeve...
The generation of concentration gradients is an essential part of a wide range of laboratory settings. However, the task usually requires tedious and repetitive steps and it is difficult to generate concentration gradients at once. Here, we present a...
It is known that in many cases a combination of drugs is more effective than single-drug treatments both for reducing toxicity and increasing efficacy. With the advent of organoid screens, personalised medicine has become possible for many diseases....
A microfluidic droplet-storage array that is capable of the continuous operation of droplet formation, storing, repositioning, retrieving, injecting and restoring is demonstrated. The microfluidic chip comprised four valve-assisted droplet generators...
We describe an automated robotic technique called direct-dispense to fabricate a polymeric platform that supports optical sensor arrays. Direct-dispense, which is a type of the emerging direct-write microfabrication techniques, uses fugitive organic...
High-throughput screening is a potent technique to accelerate the discovery and development of new materials. By performing massive synthesis and characterization processes in parallel, it can rapidly discover materials with desired components, struc...
The dispensing resolution of high-viscosity liquid is essential for adhesive micro-bonding. In comparison with the injection technique, the transfer printing method appears to be promising. Herein, an analytical model was developed to describe the dy...
The rapid quantification of nitrate nitrogen concentration plays a pivotal role in monitoring soil nutrient content. Nevertheless, the low detection efficiency limits the application of traditional methods in rapid testing. For this investigation, we...
The use of multiphase flows in microfluidics to carry dispersed phase material (droplets, particles, bubbles, or fibers) has many applications. In this review paper, we focus on such flows on centrifugal microfluidic platforms and present different m...
Mouse brain slices are one of the most common models to study brain development and functioning, increasing the number of study models that integrate microfluidic systems for hippocampal slice cultures. This report presents an alternative brain slice...
Advances in 3D printing have enabled the use of this technology in a growing number of fields, and have started to spark the interest of biologists. Having the particularity of being cell friendly and allowing multimaterial deposition, extrusion-base...
Digital microfluidics based on electrowetting on dielectric (EWOD) devices has potential as a fundamental technology for the accurate preparation of dangerous reagents, the high-speed dispensing of rapidly deteriorating reagents, and the fine adjustm...
Printed electrochemical biosensors have recently gained increasing relevance in fields ranging from basic research to home-based point-of-care. Thus, they represent a unique opportunity to enable low-cost, fast, non-invasive and/or continuous monitor...
Droplet microfluidics (DMF) is a fluidic handling technology that enables precision control over dispensing and subsequent manipulation of droplets in the volume range of microliters to picoliters, on a micro-fabricated device. There are several diff...
The rapid emergence of antimicrobial resistant bacteria requires the development of new diagnostic tests. Nucleic acid-based assays determine antimicrobial susceptibility by detecting genes that encode for the resistance. In this study, we demonstrat...
Droplet-based microfluidic screening techniques can benefit from interfacing established microtiter plate-based screening and sample management workflows. Interfacing tools are required both for loading preconfigured microtiter-plate (MTP)-based samp...
Electrowetting-on-dielectric (EWOD) is a microfluidic technology used for manipulating liquid droplets at microliter to nanoliter scale. EWOD has the ability to facilitate the accurate manipulation of liquid droplets, i.e., transporting, dispensing,...
Although immunomagnetic separation is a useful sample pretreatment method that can be used to separate target pathogens from a raw sample, it is challenging to remove unbound free magnetic nanoparticles (MNPs) for colorimetric detection of target pat...
We developed a microfluidic chip integrated with nucleic acid purification and droplet-based digital polymerase chain reaction (ddPCR) modules to realize a ‘sample-in, result-out’ infectious virus diagnosis. The whole process involved pul...
Electrodeposition is a versatile technique for the fabrication of electrodes in micro-electroanalytical devices. Conductive but low-cost materials, such as copper, can be coated with functional yet higher-cost materials such as gold or silver using e...
PCR is indispensable in basic science and biotechnology for in-orbit life science research. However, manpower and resources are limited in space. To address the constraints of in-orbit PCR, we proposed an oscillatory-flow PCR technique based on biaxi...
Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in mycoplasma pneumoniae in China. Rapid and accurate identification of mycoplasma pneumonia...
With the increasing demand for fast, accurate, and reliable biological sensor systems, miniaturized systems have been aimed at droplet-based sensor systems and have been promising. A micro-electrode dot array (MEDA) biochip, which is one kind of the...
This paper reports on a chip cooling solution using polydimethylsiloxane (PDMS) based microfluidic devices filled with n-Octadecane. A thick SU-8 layer of 150 µm is used as the master mold for patterning PDMS fabrication. With the SU-8 mold, patterns...
A prototype aerosol detection system is presented that is designed to accurately and quickly measure the concentration of selected inorganic ions in the atmosphere. The aerosol detection system combines digital microfluidics technology, aerosol impac...
Quantitative, reverse transcription, polymerase chain reaction (qRT-PCR) is facilitated by leveraging droplet microfluidic (DMF) system, which due to its precision dispensing and sample handling capabilities at microliter and lower volumes has emerge...
Three-dimensional printing at the micro-/nanoscale represents a new challenge in research and development to achieve direct printing down to nanometre-sized objects. Here, FluidFM, a combination of microfluidics with atomic force microscopy, offers a...
Due to the extremely low Reynolds number, the mixing of substances in laminar flow within microfluidic channels primarily relies on slow intermolecular diffusion, whereas various rapid reaction and detection requirements in lab-on-a-chip applications...