A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae
Abstract
:1. Introduction
2. Materials and Methods
2.1. Device Design and Fabrication
2.2. System Setup and Workflow
2.3. Analyzing the Effects of Materials and Coatings on PCR
2.4. qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae
2.5. Sensitivity of Detection System
3. Results
3.1. Characterization of Bonding Strength and Microvalve Performance
3.2. Effects of Materials and Coatings on PCR
3.3. Sample Preparation
3.4. Macrolide-Resistant Mutations Detection
3.5. Detection of the Clinical Samples
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Primer | Probe | Other | Sample Treated by the Device | |
---|---|---|---|---|
Tube 1 (conserved sequence) | P1 gene | P1 gene | / | Plasmid/clinical sample |
Tube 2 (A2063G mutation) | 23S rRNA | A2063G | / | plasmid/clinical sample |
Tube 3 (A2064G mutation) | 23S rRNA | A2064G | / | plasmid/clinical sample |
Tube 4 (positive control) | 23S rRNA | A2063G | A2063G plasmid | plasmid/clinical sample |
Tube 5 (negative control) | 23S rRNA | A2063G | water | / |
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Wang, A.; Wu, Z.; Huang, Y.; Zhou, H.; Wu, L.; Jia, C.; Chen, Q.; Zhao, J. A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae . Biosensors 2021, 11, 427. https://doi.org/10.3390/bios11110427
Wang A, Wu Z, Huang Y, Zhou H, Wu L, Jia C, Chen Q, Zhao J. A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae . Biosensors. 2021; 11(11):427. https://doi.org/10.3390/bios11110427
Chicago/Turabian StyleWang, Anyan, Zhenhua Wu, Yuhang Huang, Hongbo Zhou, Lei Wu, Chunping Jia, Qiang Chen, and Jianlong Zhao. 2021. "A 3D-Printed Microfluidic Device for qPCR Detection of Macrolide-Resistant Mutations of Mycoplasma pneumoniae " Biosensors 11, no. 11: 427. https://doi.org/10.3390/bios11110427