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  • Article
  • Open Access
21 Citations
3,622 Views
11 Pages

Frequency of Detection and Prevalence Factors Associated with Common Respiratory Pathogens in Equids with Acute Onset of Fever and/or Respiratory Signs (2008–2021)

  • Nicola Pusterla,
  • Kaitlyn James,
  • Samantha Barnum,
  • Fairfield Bain,
  • D. Craig Barnett,
  • Duane Chappell,
  • Earl Gaughan,
  • Bryant Craig,
  • Chrissie Schneider and
  • Wendy Vaala

A voluntary biosurveillance program was established in 2008 in order to determine the shedding frequency and prevalence factors for common respiratory pathogens associated with acute onset of fever and/or respiratory signs in equids from the USA. Ove...

  • Article
  • Open Access
15 Citations
2,228 Views
8 Pages

Frequency of Detection of Respiratory Pathogens in Clinically Healthy Show Horses Following a Multi-County Outbreak of Equine Herpesvirus-1 Myeloencephalopathy in California

  • Abigail Wilcox,
  • Samantha Barnum,
  • Cara Wademan,
  • Rachel Corbin,
  • Edlin Escobar,
  • Emir Hodzic,
  • Stephen Schumacher and
  • Nicola Pusterla

8 October 2022

Actively shedding healthy horses have been indicated as a possible source of respiratory pathogen outbreak, transmission, and spread. Using nasal swabs from clinically healthy sport horses submitted for qPCR testing after an outbreak of equine herpes...

  • Review
  • Open Access
8 Citations
2,178 Views
19 Pages

Advances in Donkey Disease Surveillance and Microbiome Characterization in China

  • Muhammad Zahoor Khan,
  • Yan Li,
  • Mingxia Zhu,
  • Mengmeng Li,
  • Tongtong Wang,
  • Zhenwei Zhang,
  • Wenqiang Liu,
  • Qingshan Ma and
  • Changfa Wang

This review article highlights the surveillance of bacterial, viral, and parasitic diseases in donkey populations in China. Key findings highlight significant threats from Equine herpesviruses (EHV-8 and EHV-1), which cause encephalitis, abortion, an...

  • Article
  • Open Access
3 Citations
2,652 Views
9 Pages

A Pilot Serosurvey for Selected Pathogens in Feral Donkeys (Equus asinus)

  • Erin L. Goodrich,
  • Amy McLean and
  • Cassandra Guarino

2 October 2020

Recent removal and relocation of feral donkeys from vast public lands to more concentrated holding pens, training facilities, and offsite adoption locations raises several health and welfare concerns. Very little is known regarding the common equid p...

  • Article
  • Open Access
13 Citations
2,614 Views
8 Pages

19 October 2021

Three newly identified equine parvoviruses (equine parvovirus hepatitis (EqPV-H), equine parvovirus CSF (EqPV-CSF) and equine copivirus (Eqcopivirus)) have recently been discovered in horses with respiratory signs. However, the clinical impact of the...

  • Article
  • Open Access
560 Views
14 Pages

Molecular Detection of Theileria equi, Babesia caballi, and Borrelia burgdorferi Sensu Lato in Hippobosca equina from Horses in Spain

  • Abel Dorrego,
  • Sergi Olvera-Maneu,
  • Eduard Jose-Cunilleras,
  • Paloma Gago,
  • Alejandra Raez,
  • Belen Rivera,
  • Ariana Oporto,
  • Sergio Gonzalez and
  • Fatima Cruz-Lopez

15 January 2026

The forest fly (Hippobosca equina) is an obligate haematophagous dipteran insect (order Diptera) that primarily infests horses and may contribute to the circulation of vector-borne pathogens. This study aimed to investigate the presence of Anaplasma ...

  • Article
  • Open Access
15 Citations
6,373 Views
8 Pages

Investigation of the Role of Healthy and Sick Equids in the COVID-19 Pandemic through Serological and Molecular Testing

  • Kaila O. Y. Lawton,
  • Rick M. Arthur,
  • Benjamin C. Moeller,
  • Samantha Barnum and
  • Nicola Pusterla

28 February 2022

More and more studies are reporting on the natural transmission of SARS-CoV-2 between humans with COVID-19 and their companion animals (dogs and cats). While horses are apparently susceptible to SARS-CoV-2 infection based on the homology between the...

  • Article
  • Open Access
4 Citations
2,161 Views
14 Pages

10 June 2025

Monitoring infectious diseases is essential for safeguarding equine health and ensuring the sustainability of the horse industry. In 2019, the Royal Veterinary Association of the Netherlands (KNMvD) and Royal GD (GD Animal Health) launched SEIN (Surv...

  • Article
  • Open Access
2 Citations
961 Views
13 Pages

Evaluation of Celastrol Antiviral Activity Against Equid Alphaherpesvirus Type 8 Infection

  • Yue Yu,
  • Jiayu Wang,
  • Lian Ruan,
  • Li Chen,
  • Muhammad Zahoor Khan,
  • Anrong You,
  • Changfa Wang,
  • Liangliang Li,
  • Huiying Ren and
  • Wenhua Liu
  • + 1 author

28 February 2025

Equid alphaherpesvirus type 8 (EHV-8) is a contagious pathogen that causes reproductive disorders, respiratory diseases, and viral encephalitis in equids, resulting in significant economic losses for the global horse and donkey industries. Currently,...

  • Article
  • Open Access
2,099 Views
12 Pages

Detection of Burkholderia mallei in Microbiological Culture: A Comparative Analysis of PCR Primer Sets

  • Jéssica Cristine K. Moriya,
  • Paula Adas P. Suniga,
  • Ana Clara L. Araújo,
  • Maria Goretti Santos,
  • Juliana S. G. Rieger,
  • Cynthia Mantovani,
  • Rodrigo Jardim,
  • Márcio Roberto Silva,
  • Flábio R. Araújo and
  • Lenita R. Santos

Glanders is a highly contagious and often fatal zoonotic disease of equids caused by Burkholderia mallei, a pathogen of significant concern due to its potential for bioterrorism. In Brazil, glanders remains endemic, particularly among working equids...

  • Review
  • Open Access
23 Citations
6,615 Views
23 Pages

Current Progress of Avian Vaccines Against West Nile Virus

  • Nereida Jiménez de Oya,
  • Estela Escribano-Romero,
  • Ana-Belén Blázquez,
  • Miguel A. Martín-Acebes and
  • Juan-Carlos Saiz

23 September 2019

Birds are the main natural host of West Nile virus (WNV), the worldwide most distributed mosquito-borne flavivirus, but humans and equids can also be sporadic hosts. Many avian species have been reported as susceptible to WNV, particularly corvids. I...

  • Systematic Review
  • Open Access
3 Citations
3,257 Views
21 Pages

In equine stables and their surroundings, a large number of insects are present that can be a nuisance to their equine hosts. Previous studies about dipterans transmitting infectious agents to Equidae have largely focused on Nematocera. For the prepa...

  • Article
  • Open Access
12 Citations
2,948 Views
12 Pages

Voluntary Surveillance Program for Equine Influenza Virus in the United States during 2008–2021

  • Duane E. Chappell,
  • D. Craig Barnett,
  • Kaitlyn James,
  • Bryant Craig,
  • Fairfield Bain,
  • Earl Gaughan,
  • Chrissie Schneider,
  • Wendy Vaala,
  • Samantha M. Barnum and
  • Nicola Pusterla

27 January 2023

A voluntary upper respiratory biosurveillance program in the USA received 9740 nasal swab submissions during the years 2008–2021 from 333 veterinarians and veterinary clinics. The nasal swabs were submitted for qPCR testing for six common upper...

  • Article
  • Open Access
31 Citations
3,836 Views
23 Pages

Identification of a New Equid Herpesvirus 1 DNA Polymerase (ORF30) Genotype with the Isolation of a C2254/H752 Strain in French Horses Showing no Major Impact on the Strain Behaviour

  • Gabrielle Sutton,
  • Côme Thieulent,
  • Christine Fortier,
  • Erika S. Hue,
  • Christel Marcillaud-Pitel,
  • Alexis Pléau,
  • Alain Deslis,
  • Edouard Guitton,
  • Romain Paillot and
  • Stéphane Pronost

13 October 2020

Equid herpesvirus 1 is one of the most common viral pathogens in the horse population and is associated with respiratory disease, abortion and still-birth, neonatal death and neurological disease. A single point mutation in the DNA polymerase gene (O...

  • Article
  • Open Access
11 Citations
2,642 Views
21 Pages

Association of SFG Rickettsia massiliae and Candidatus Rickettsia shennongii with Different Hard Ticks Infesting Livestock Hosts

  • Shehla Shehla,
  • Farman Ullah,
  • Abdulaziz Alouffi,
  • Mashal M. Almutairi,
  • Zaibullah Khan,
  • Tetsuya Tanaka,
  • Marcelo B. Labruna,
  • Kun-Hsien Tsai and
  • Abid Ali

24 August 2023

Ixodid ticks are responsible for the transmission of various intracellular bacteria, such as the Rickettsia species. Little Information is available about the genetic characterization and epidemiology of Rickettsia spp. The current study was designed...

  • Article
  • Open Access
6 Citations
1,904 Views
10 Pages

7 July 2024

Background: Equid alphaherpesvirus 1 (EHV-1) is a highly contagious respiratory tract pathogen of horses, and infection may be followed by myeloencephalopathy or abortion. Surveillance and early detection have focused on PCR assays using less tolerat...

  • Communication
  • Open Access
9 Citations
4,003 Views
14 Pages

13 September 2019

Equid alphaherpesvirus 1 (EHV-1) is an important pathogen of horses. It is spread worldwide and causes significant economic losses. The ORF33 gene has a conserved region that is often used as target in diagnostic PCR protocols. Single nucleotide poin...

  • Article
  • Open Access
9 Citations
3,121 Views
9 Pages

Experimental Infection of Horses with Influenza D Virus

  • Chithra C. Sreenivasan,
  • Tirth Uprety,
  • Stephanie E. Reedy,
  • Gun Temeeyasen,
  • Ben M. Hause,
  • Dan Wang,
  • Feng Li and
  • Thomas M. Chambers

23 March 2022

Antibodies to influenza D virus (IDV) have been detected in horses, but no evidence of disease in the field has been reported. To determine whether IDV is infectious, immunogenic, and pathogenic in horses, four 2-year-old horses seronegative for both...

  • Article
  • Open Access
3 Citations
2,283 Views
16 Pages

A Screening Study Identified Decitabine as an Inhibitor of Equid Herpesvirus 4 That Enhances the Innate Antiviral Response

  • Camille Normand,
  • Côme J. Thieulent,
  • Christine Fortier,
  • Gabrielle Sutton,
  • Catherine Senamaud-Beaufort,
  • Laurent Jourdren,
  • Corinne Blugeon,
  • Pierre-Olivier Vidalain,
  • Stéphane Pronost and
  • Erika S. Hue

8 May 2024

Equid herpesvirus 4 (EHV-4) is a common respiratory pathogen in horses. It sporadically induces abortion or neonatal death. Although its contribution in neurological disorders is not clearly demonstrated, there is a strong suspicion of its involvemen...

  • Article
  • Open Access
15 Citations
3,142 Views
9 Pages

A Sentinel Serological Study in Selected Zoo Animals to Assess Early Detection of West Nile and Usutu Virus Circulation in Slovenia

  • Pavel Kvapil,
  • Joško Račnik,
  • Marjan Kastelic,
  • Eva Bártová,
  • Miša Korva,
  • Mateja Jelovšek and
  • Tatjana Avšič-Županc

6 April 2021

Monitoring infectious diseases is a crucial part of preventive veterinary medicine in zoological collections. This zoo environment contains a great variety of animal species that are in contact with wildlife species as a potential source of infectiou...

  • Communication
  • Open Access
1,615 Views
9 Pages

The Role of Viral Pathogens in Horse Respiratory Diseases: A Cytological and Molecular Approach Using Next-Generation Sequencing

  • Maurizio Mazzei,
  • Benedetta Sorvillo,
  • Micaela Sgorbini,
  • Francesca Bindi,
  • Alice Perelli and
  • Fulvio Laus

21 November 2024

(1) Background: Respiratory problems may be associated with pathogens among which viruses may play an important role in causing or promoting clinical signs. However, traditional diagnostic methods for equine infectious diseases, such as PCR, are limi...

  • Article
  • Open Access
1 Citations
1,525 Views
15 Pages

Breaking Latent Infection: How ORF37/38-Deletion Mutants Offer New Hope against EHV-1 Neuropathogenicity

  • Yue Hu,
  • Si-Yu Zhang,
  • Wen-Cheng Sun,
  • Ya-Ru Feng,
  • Hua-Rui Gong,
  • Duo-Liang Ran,
  • Bao-Zhong Zhang and
  • Jian-Hua Liu

16 September 2024

Equid alphaherpesvirus 1 (EHV-1) has been linked to the emergence of neurological disorders, with the horse racing industry experiencing significant impacts from outbreaks of equine herpesvirus myeloencephalopathy (EHM). Building robust immune memory...

  • Article
  • Open Access
18 Citations
3,805 Views
11 Pages

Surveillance of Zoonotic Parasites in Animals Involved in Animal-Assisted Interventions (AAIs)

  • Giulia Simonato,
  • Patrizia Danesi,
  • Antonio Frangipane di Regalbono,
  • Giorgia Dotto,
  • Cinzia Tessarin,
  • Mario Pietrobelli and
  • Daniela Pasotto

Animal-assisted interventions (AAIs) are based on the establishment of a therapeutic relationship between animals and beneficiaries that is certain to provide positive effects, while currently, it reads as if AAIs aim at exposing stakeholders to pote...

  • Feature Paper
  • Article
  • Open Access
4 Citations
2,972 Views
14 Pages

Streptococcus equi subsp. zooepidemicus: Epidemiological and Genomic Findings of an Emerging Pathogen in Central Italy

  • Francesca Cito,
  • Cristina Esmeralda Di Francesco,
  • Daniela Averaimo,
  • Alexandra Chiaverini,
  • Alessandra Alessiani,
  • Marco Di Domenico,
  • Marta Cresci,
  • Marco Rulli,
  • Maria Chiara Cantelmi and
  • Antonio Petrini
  • + 3 authors

8 May 2025

Streptococcus equi subsp. zooepidemicus (SEZ) is a major problem in equine veterinary medicine. Typically, a commensal in horses, SEZ can cause severe disease including respiratory infections, septicaemia and reproductive tract infections under certa...

  • Article
  • Open Access
12 Citations
6,059 Views
10 Pages

Low Prevalence of Enzootic Equine Influenza Virus among Horses in Mongolia

  • Alexandra Sack,
  • Ulziimaa Daramragchaa,
  • Maitsetseg Chuluunbaatar,
  • Battsetseg Gonchigoo,
  • Boldbaatar Bazartseren,
  • Nyamdorj Tsogbadrakh and
  • Gregory C. Gray

30 November 2017

Horses are critically important for Mongolian herders’ livelihoods, providing transportation and food products, and playing important cultural roles. Equine influenza virus (EIV) epizootics have been frequent among Mongolia’s horses, with five occurr...

  • Review
  • Open Access
6 Citations
4,514 Views
18 Pages

Influenza A and D Viruses in Non-Human Mammalian Hosts in Africa: A Systematic Review and Meta-Analysis

  • Annie Kalonda,
  • Marvin Phonera,
  • Ngonda Saasa,
  • Masahiro Kajihara,
  • Catherine G. Sutcliffe,
  • Hirofumi Sawa,
  • Ayato Takada and
  • Edgar Simulundu

2 December 2021

We conducted a systematic review and meta-analysis to investigate the prevalence and current knowledge of influenza A virus (IAV) and influenza D virus (IDV) in non-human mammalian hosts in Africa. PubMed, Google Scholar, Wiley Online Library and Wor...

  • Article
  • Open Access
1 Citations
3,362 Views
13 Pages

Differential Expression of Immune Genes in the Rhipicephalus microplus Gut in Response to Theileria equi Infection

  • Patrícia Gonzaga Paulino,
  • Maristela Peckle,
  • Leo Paulis Mendonça,
  • Carlos Luiz Massard,
  • Sandra Antunes,
  • Joana Couto,
  • Ana Domingos,
  • Daniel da Silva Guedes Junior,
  • Alejandro Cabezas-Cruz and
  • Huarrisson Azevedo Santos

6 December 2022

Rhipicephalus microplus is the only tick species known to serve as a biological vector of Theileria equi for horses and other equids in Brazil. The protozoan T. equi is one of the causal agents of equine piroplasmosis, a major threat in horse breedin...

  • Article
  • Open Access
3 Citations
2,388 Views
20 Pages

Modulation of Equid Herpesvirus-1 Replication Dynamics In Vitro Using CRISPR/Cas9-Assisted Genome Editing

  • Rabab T. Hassanien,
  • Côme J. Thieulent,
  • Mariano Carossino,
  • Ganwu Li and
  • Udeni B. R. Balasuriya

6 March 2024

(1) Background: equid alphaherpesvirus-1 (EHV-1) is a highly contagious viral pathogen prevalent in most horse populations worldwide. Genome-editing technologies such as CRISPR/Cas9 have become powerful tools for precise RNA-guided genome modificatio...

  • Article
  • Open Access
13 Citations
3,827 Views
12 Pages

Severe Neurologic Disease in a Horse Caused by Tick-Borne Encephalitis Virus, Austria, 2021

  • Phebe de Heus,
  • Zoltán Bagó,
  • Pia Weidinger,
  • Dilara Lale,
  • Dagmar S. Trachsel,
  • Sandra Revilla-Fernández,
  • Kaspar Matiasek and
  • Norbert Nowotny

29 September 2023

As evidenced by sero-epidemiological studies, infections of horses with the tick-borne encephalitis virus (TBEV) occur frequently in TBEV-endemic areas. However, there are only very few reports of clinical cases. A possible underreporting may be due...

  • Article
  • Open Access
386 Views
23 Pages

27 January 2026

Comparative transcriptomics offers a powerful approach to elucidate host–virus interactions across related pathogens, yet systematic evaluations across species-matched cellular systems remain limited. We performed a cross-species RNA sequencing...

  • Article
  • Open Access
9 Citations
2,962 Views
17 Pages

Oral Administration of Valganciclovir Reduces Clinical Signs, Virus Shedding and Cell-Associated Viremia in Ponies Experimentally Infected with the Equid Herpesvirus-1 C2254 Variant

  • Côme J. Thieulent,
  • Gabrielle Sutton,
  • Marie-Pierre Toquet,
  • Samuel Fremaux,
  • Erika Hue,
  • Christine Fortier,
  • Alexis Pléau,
  • Alain Deslis,
  • Stéphane Abrioux and
  • Romain Paillot
  • + 2 authors

Equid alphaherpesvirus-1 (EHV-1) is one of the main pathogens in horses, responsible for respiratory diseases, ocular diseases, abortions, neonatal foal death and neurological complications such as equine herpesvirus myeloencephalopathy (EHM). Curren...

  • Article
  • Open Access
2 Citations
2,989 Views
21 Pages

Equine Arteritis Virus in Monocytic Cells Suppresses Differentiation and Function of Dendritic Cells

  • Nathifa A. Moyo,
  • Dave Westcott,
  • Rachel Simmonds and
  • Falko Steinbach

16 January 2023

Equine viral arteritis is an infectious disease of equids caused by equine arteritis virus (EAV), an RNA virus of the family Arteriviridae. Dendritic cells (DC) are important modulators of the immune response with the ability to present antigen to na...

  • Review
  • Open Access
13 Citations
5,563 Views
16 Pages

The Use of Innovative Diagnostics to Inform Sustainable Control of Equine Helminth Infections

  • Jacqueline B. Matthews,
  • Natalia Peczak and
  • Kirsty L. Lightbody

11 October 2023

Helminths are commonly found in grazing equids, with cyathostomin nematodes and the cestode Anoplocephala perfoliata being the most prevalent. Most horses harbour low burdens of these parasites and do not develop signs of infection; however, in a sma...

  • Article
  • Open Access
6 Citations
2,894 Views
13 Pages

The Prevalence and Molecular Biology of Staphylococcus aureus Isolated from Healthy and Diseased Equine Eyes in Egypt

  • Amin Tahoun,
  • Helmy K. Elnafarawy,
  • Hanem El-Sharkawy,
  • Amira M. Rizk,
  • Mohammed Alorabi,
  • Ahmed M. El-Shehawi,
  • Mohamed A. Youssef,
  • Hussam M. M. Ibrahim and
  • Sabry El-Khodery

10 February 2022

This work aimed to characterize S. aureus isolates from the eyes of healthy and clinically affected equines in the Kafrelsheikh Governorate, Egypt. A total of 110 animals were examined for the presence of S. aureus, which was isolated from 33 animals...

  • Article
  • Open Access
4 Citations
5,264 Views
18 Pages

Molecular Identification of Selected Tick-Borne Protozoan and Bacterial Pathogens in Thoroughbred Racehorses in Cavite, Philippines

  • Eloiza May Galon,
  • Adrian Miki Macalanda,
  • Mary Margarett Garcia,
  • Chrysler James Ibasco,
  • Anatolio Garvida,
  • Shengwei Ji,
  • Iqra Zafar,
  • Yae Hasegawa,
  • Mingming Liu and
  • Xuenan Xuan
  • + 4 authors

13 October 2021

Tick-borne diseases (TBDs) considerably impair equine health and productivity. Moreover, TBDs, particularly equine piroplasmosis, impede international movement and trade of equids, which is a vital component of the global horse racing industry. In th...

  • Article
  • Open Access
22 Citations
5,263 Views
17 Pages

Kinetoplastids are protozoa containing a range of ubiquitous free_living species–pathogens of invertebrates, vertebrates and even some plants. Some of them are causative agents of canine vector-borne diseases. Their diagnosis is often missing i...

  • Article
  • Open Access
15 Citations
2,995 Views
15 Pages

Theileria terrestris nov. sp.: A Novel Theileria in Lowland Tapirs (Tapirus terrestris) from Two Different Biomes in Brazil

  • Anna Claudia Baumel Mongruel,
  • Emília Patrícia Medici,
  • Ariel da Costa Canena,
  • Ana Cláudia Calchi,
  • Lívia Perles,
  • Bianca Cardenal Balla Rodrigues,
  • João Fabio Soares,
  • Rosangela Zacarias Machado and
  • Marcos Rogério André

The low-land tapir (Tapirus terrestris) is the largest wild terrestrial mammal found in Brazil. Although T. terrestris has been already reported as a host of hemoparasites, the occurrence and genetic identity of Piroplasmida agents in this species is...

  • Review
  • Open Access
165 Citations
19,404 Views
27 Pages

European Surveillance for West Nile Virus in Mosquito Populations

  • Olivier Engler,
  • Giovanni Savini,
  • Anna Papa,
  • Jordi Figuerola,
  • Martin H. Groschup,
  • Helge Kampen,
  • Jolyon Medlock,
  • Alexander Vaux,
  • Anthony J. Wilson and
  • Nicholas Johnson
  • + 18 authors

A wide range of arthropod-borne viruses threaten both human and animal health either through their presence in Europe or through risk of introduction. Prominent among these is West Nile virus (WNV), primarily an avian virus, which has caused multiple...