Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples
2.2. PCR and Sequencing
2.3. Sequence Analysis
3. Results
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
- OIE-Listed Diseases, Infections and Infestations in Force in 2019. Available online: www.oie.int (accessed on 27 July 2019).
- Telford, E.A.; Watson, M.S.; McBride, K.; Davison, A.J. The DNA sequence of equine herpesvirus-1. Virology 1992, 189, 304–316. [Google Scholar] [CrossRef]
- OIE (World Organization for Animal Health). Chapter 2.5.9: Equine rhinopneumonitis (infection with equid herpesvirus-1 and -4). In OIE Terrestrial Manual; OIE: Paris, France, 2017. [Google Scholar]
- Nugent, J.; Birch-Machin, I.; Smith, K.C.; Mumford, J.A.; Swann, Z.; Newton, J.R.; Bowden, R.J.; Allen, G.P.; Davis-Poynter, N. Analysis of Equid Herpesvirus 1 Strain Variation Reveals a Point Mutation of the DNA Polymerase Strongly Associated with Neuropathogenic versus Nonneuropathogenic Disease Outbreaks. J. Virol. 2006, 80, 4047–4060. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Allen, G.P.; Breathnach, C.C. Quantification by real-time PCR of the magnitude and duration of leucocyte-associated viraemia in horses infected with neuropathogenic vs. non-neuropathogenic strains of EHV-1. Equine Vet. J. 2006, 38, 252–257. [Google Scholar] [CrossRef] [PubMed]
- Goodman, L.B.; Loregian, A.; Perkins, G.A.; Nugent, J.; Buckles, E.L.; Mercorelli, B.; Kydd, J.H.; Palù, G.; Smith, K.C.; Osterrieder, N.; et al. A Point Mutation in a Herpesvirus Polymerase Determines Neuropathogenicity. PLoS Pathog. 2007, 3, e160. [Google Scholar] [CrossRef] [PubMed]
- Malik, P.; Balint, A.; Dan, A.; Palfi, V. Molecular characterisation of the ORF68 region of equine herpesvirus-1 strains isolated from aborted fetuses in Hungary between 1977 and 2008. Acta Vet. Hung. 2012, 60, 175–187. [Google Scholar] [CrossRef] [PubMed]
- Anagha, G.; Gulati, B.R.; Riyesh, T.; Virmani, N. Genetic characterization of equine herpesvirus 1 isolates from abortion outbreaks in India. Arch. Virol. 2017, 162, 157–163. [Google Scholar] [CrossRef] [PubMed]
- Negussie, H.; Gizaw, D.; Tessema, T.S.; Nauwynck, H.J. Equine Herpesvirus-1 Myeloencephalopathy, an Emerging Threat of Working Equids in Ethiopia. Transbound Emerg. Dis. 2017, 64, 389–397. [Google Scholar] [CrossRef]
- Stasiak, K.; Dunowska, M.; Hills, S.F.; Rola, J. Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland. Arch. Virol. 2017, 162, 2329–2335. [Google Scholar] [CrossRef]
- Matczuk, A.K.; Skarbek, M.; Jackulak, N.A.; Bazanow, B.A. Molecular characterisation of equid alphaherpesvirus 1 strains isolated from aborted fetuses in Poland. Virol. J. 2018, 15, 186. [Google Scholar] [CrossRef]
- Garvey, M.; Lyons, R.; Hector, R.D.; Walsh, C.; Arkins, S.; Cullinane, A. Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017. Pathogens 2019, 8, 7. [Google Scholar] [CrossRef]
- Bryant, N.A.; Wilkie, G.S.; Russell, C.A.; Compston, L.; Grafham, D.; Clissold, L.; McLay, K.; Medcalf, L.; Newton, R.; Davison, A.J.; et al. Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks. Transbound Emerg. Dis. 2018, 65, 817–832. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Autorino, G.L.; Corradi, V.; Frontoso, R.; Galletti, S.; Manna, G.; Mascioni, A.; Pallone, A.; Ricci, I.; Rosone, F.; Simula, M.; et al. P.3 Gestione di un focolaio neurologico da Equine herpesvirus 1 (EHV-1). In Workshop Nazionale di Virologia Veterinaria; Delogu, R., Falcone, E., Monini, M., Ruggeri, F.M., Di Martino, B., Marsilio, F., Monaco, F., Savini, G., Eds.; Istituto Superiore di Sanità: Riassunti, Roma, Italy, 2014; p. 15. [Google Scholar]
- Preziuso, S.; Cuteri, V. A Multiplex Polymerase Chain Reaction Assay for Direct Detection and Differentiation of β-Hemolytic Streptococci in Clinical Samples from Horses. J. Equine Vet. Sci. 2012, 32, 292–296. [Google Scholar] [CrossRef]
- Preziuso, S.; Pinho, M.D.; Attili, A.R.; Melo-Cristino, J.; Acke, E.; Midwinter, A.C.; Cuteri, V.; Ramirez, M. PCR based differentiation between Streptococcus dysgalactiae subsp. equisimilis strains isolated from humans and horses. Comp. Immunol. Microbiol. Infect. Dis. 2014, 37, 169–172. [Google Scholar] [CrossRef] [PubMed]
- Wang, L.; Raidal, S.L.; Pizzirani, A.; Wilcox, G.E. Detection of respiratory herpesviruses in foals and adult horses determined by nested multiplex PCR. Vet. Microbiol. 2007, 121, 18–28. [Google Scholar] [CrossRef]
- Allen, G.P. Antemortem detection of latent infection with neuropathogenic strains of equine herpesvirus-1 in horses. Am. J. Vet. Res. 2006, 67, 1401–1405. [Google Scholar] [CrossRef]
- Untergasser, A.; Nijveen, H.; Rao, X.; Bisseling, T.; Geurts, R.; Leunissen, J.A.M. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 2007, 35, W71–W74. [Google Scholar] [CrossRef] [Green Version]
- Hall, T.A. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp. Ser. 1999, 41, 95–98. [Google Scholar]
- Edgar, R.C. MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004, 32, 1792–1797. [Google Scholar] [CrossRef]
- Kumar, S.; Stecher, G.; Tamura, K. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol. Biol. Evol. 2016, 33, 1870–1874. [Google Scholar] [CrossRef] [Green Version]
- Mori, E.; Lara, d.C.C.S.H.; Cunha, E.M.S.; Villalobos, E.M.C.; Mori, C.M.C.; Soares, R.M.; Brandao, P.E.; Fernandes, W.R.; Richtzenhain, L.J. Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers. Braz. J. Microbiol. 2015, 46, 565–570. [Google Scholar] [CrossRef] [Green Version]
- Turan, N.; Yildirim, F.; Altan, E.; Sennazli, G.; Gurel, A.; Diallo, I.; Yilmaz, H. Molecular and pathological investigations of EHV-1 and EHV-4 infections in horses in Turkey. Res. Vet. Sci. 2012, 93, 1504–1507. [Google Scholar] [CrossRef]
- Perkins, G.A.; Goodman, L.B.; Tsujimura, K.; Van de Walle, G.R.; Kim, S.G.; Dubovi, E.J.; Osterrieder, N. Investigation of the prevalence of neurologic equine herpes virus type 1 (EHV-1) in a 23-year retrospective analysis (1984–2007). Vet. Microbiol. 2009, 139, 375–378. [Google Scholar] [CrossRef]
- Vissani, M.A.; Becerra, M.L.; Olguín Perglione, C.; Tordoya, M.S.; Miño, S.; Barrandeguy, M. Neuropathogenic and non-neuropathogenic genotypes of Equid Herpesvirus type 1 in Argentina. Vet. Microbiol. 2009, 139, 361–364. [Google Scholar] [CrossRef] [PubMed]
- Pronost, S.; Leon, A.; Legrand, L.; Fortier, C.; Miszczak, F.; Freymuth, F.; Fortier, G. Neuropathogenic and non-neuropathogenic variants of equine herpesvirus 1 in France. Vet. Microbiol. 2010, 145, 329–333. [Google Scholar] [CrossRef] [PubMed]
- Smith, K.L.; Allen, G.P.; Branscum, A.J.; Frank Cook, R.; Vickers, M.L.; Timoney, P.J.; Balasuriya, U.B.R. The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. Vet. Microbiol. 2010, 141, 5–11. [Google Scholar] [CrossRef] [Green Version]
- Fritsche, A.K.; Borchers, K. Detection of neuropathogenic strains of Equid Herpesvirus 1 (EHV-1) associated with abortions in Germany. Vet. Microbiol. 2011, 147, 176–180. [Google Scholar] [CrossRef]
- Tsujimura, K.; Oyama, T.; Katayama, Y.; Muranaka, M.; Bannai, H.; Nemoto, M.; Yamanaka, T.; Kondo, T.; Kato, M.; Matsumura, T. Prevalence of equine herpesvirus type 1 strains of neuropathogenic genotype in a major breeding area of Japan. J. Vet. Med. Sci. 2011, 73, 1663–1667. [Google Scholar] [CrossRef]
- Castro, E.R.; Arbiza, J. Detection and genotyping of equid herpesvirus 1 in Uruguay. Rev. Sci. Tech. 2017, 36, 799–806. [Google Scholar] [CrossRef]
- Damiani, A.M.; de Vries, M.; Reimers, G.; Winkler, S.; Osterrieder, N. A severe equine herpesvirus type 1 (EHV-1) abortion outbreak caused by a neuropathogenic strain at a breeding farm in northern Germany. Vet. Microbiol. 2014, 172, 555–562. [Google Scholar] [CrossRef] [PubMed]
- Vaz, P.K.; Horsington, J.; Hartley, C.A.; Browning, G.F.; Ficorilli, N.P.; Studdert, M.J.; Gilkerson, J.R.; Devlin, J.M. Evidence of widespread natural recombination among field isolates of equine herpesvirus 4 but not among field isolates of equine herpesvirus 1. J. Gen. Virol. 2016, 97, 747–755. [Google Scholar] [CrossRef] [PubMed]
- Guo, X.; Izume, S.; Okada, A.; Ohya, K.; Kimura, T.; Fukushi, H. Full genome sequences of zebra-borne equine herpesvirus type 1 isolated from zebra, onager and Thomson’s gazelle. J. Vet. Med. Sci. 2014, 76, 1309–1312. [Google Scholar] [CrossRef] [PubMed]
- Said, A.; Damiani, A.; Osterrieder, N. Ubiquitination and degradation of the ORF34 gene product of equine herpesvirus type 1 (EHV-1) at late times of infection. Virology 2014, 460–461, 11–22. [Google Scholar] [CrossRef] [PubMed]
- Shakya, A.K.; O’Callaghan, D.J.; Kim, S.K. Comparative Genomic Sequencing and Pathogenic Properties of Equine Herpesvirus 1 KyA and RacL11. Front. Vet. Sci. 2017, 4, 211. [Google Scholar] [CrossRef] [PubMed] [Green Version]
Code | Stable | Source | Vaccination |
---|---|---|---|
08m27 | A | Organs | no |
08m160 | B | Organs | yes |
09m34T | B | Organs | yes |
09m45 | C | NS | yes |
09m68 | D | NS | yes |
09m142 | E | NS | yes |
09m209 | E | NS | yes |
09m217 | F | NS | yes |
10m01 | G | Organs | no |
10m106 | J | Organs | yes |
17m07 | J | Organs | yes |
17m13 | K | Organs | yes |
17m15 | K | NS | yes |
18m30 | F | Organs | yes |
19m04 | E | Organs | no |
19m05 | C | Organs | no |
19m08 | L | CSF | no |
19m10 | M | Organs | yes |
19m13 | N | Organs | no |
19m14 | M | Organs | no |
Gene | Primer Name | Sequence (5′–3′) | Product Size (bp) | Annealing Temperature (°C) | Reference |
---|---|---|---|---|---|
ORF30 | F8 | GTGGACGGTACCCCGGAC | 380 | 60 | [18] |
R2 | GTGGGGATTCGCGCCCTCACC | ||||
F7 * | GGGAGCAAAGGTTCTAGACC | 256 | 60 | [18] | |
R3 * | AGCCAGTCGCGCAGCAAGATG | ||||
ORF33 | FC2 | CTTGTGAGATCTAACCGCAC | 1181 | 60 | [17] |
RC | GGGTATAGAGCTTTCATGGG | ||||
FC2int * | CCGCACCTACGACCTAAAAA | 940 | 58 | This study | |
RCint * | CGATCCCCTGCATAATCACT | ||||
ORF34 | 1058F | GGCCCCAAGGATATTTAAGC | 855 | 58 | This study |
1893R | GTTTGAGGCGGTTACGTCAG | ||||
1090Fi * | CCGAGGTTTCATCCTCATTC | 714 | 58 | This study | |
1784Ri * | GCGGACATATTCGTGTCTCA | ||||
ORF68 | 68p1-Fe | AAGCATTGCCAAACAGTTCC | 846 | 55 | This study |
68p1-Re | CGAACACTCCCCAGAGTAGG | ||||
68p2-Fi * | TGAGCCGACAATGTTTCGTA | 774 | 57 | This study | |
68p2-Ri * | GTTCCATCCACGTCACGTCT |
Code | ORF33 SNPs | |||||
---|---|---|---|---|---|---|
nt 1526 | nt 1531 | nt 2391 | nt 2429 | nt 2513 | nt 2583 | |
Ab4 | A | A | G | A | A | C |
08m27 | A | G | G | - | - | - |
08m160 | unsp. | |||||
09m34 | T | A | A | A | A | C |
09m45 | T | A | A | A | A | C |
09m68 | T | A | A | - | - | - |
09m142 | T | A | A | A | A | C |
09m209 | unsp. | |||||
09m217 | unsp. | |||||
10m01 | A | G | G | - | - | - |
10m106 | T | A | A | - | - | - |
17m07 | - | - | - | - | - | - |
17m13 | T | A | A | - | - | - |
17m15 | T | A | A | - | - | - |
18m30 | - | - | - | - | - | - |
19m04 | - | - | - | - | - | - |
19m05 | - | - | - | - | - | - |
19m08 | - | - | - | - | - | - |
19m10 | A | A | G | G | C | G |
19m13 | - | - | - | - | - | - |
19m14 | - | - | - | - | - | - |
Group | 33 | 60 | 62 | 71 | 73 | 81 | 95 | 104 | 110 | 115 | 148 | 149 | 156 | 159 | 197 | 216 | 256 | 257 | 282 | 285 | 303 | 304 | 317 | 380 | 391 | 402 | 405 | 410 | 414 | 422 | 428 | 477 | N | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AY665713.1_Ab4 | 1 | C | T | C | C | G | G | C | T | G | G | A | C | G | A | A | G | G | C | T | A | C | T | C | C | T | C | A | T | T | C | C | G | 70 |
09m142 | 1 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
09m209 | 2 | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 |
09m34 | 2 | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
17m15 | 2 | . | C | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | C * | . | . | . | . | |
KU206455.1_Buckin.93/2011 | 3 | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 5 |
KF644568.1_NMKT04 | 3 | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
KF644579.1_89c25 | 4 | . | . | . | . | A * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2 |
KF644577.1_89c105 | 4 | . | . | . | . | A * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
19m04 | 5 | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 0 |
19m05 | 5 | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
MF975655.1_KyA | 6 | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 4 |
KF644570.1_NY05 | 6 | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
KT324724.1_NZA-77 | 7 | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | . | 6 |
KU206475.1_Lincoln.2/2012 | 7 | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | A | . | . | . | . | . | . | . | . | . | . | . | |
KU206453.1_Kent/43/1994 | 8 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 4 |
AY464052.1_V592 | 8 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | G * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
KF644578.1_01c1 | 9 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | 2 |
KF644566.1_90c16 | 9 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
KU206470.1_Oxfor.206/2013 | 10 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | G | . | . | . | . | . | 10 |
KM593996.1_T953 | 10 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | . | . | . | G | . | . | . | . | . | |
KT324733.1_717A-82 | 11 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | 2 |
KT324725.1_3045-07 | 11 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C | . | . | . | |
KF644580.1_T-529_10/84 | 12 | T | . | . | A * | . | A | . | C * | . | C * | G * | . | . | G | . | A | . | . | C | . | . | . | . | . | . | T | . | . | . | . | . | A | 3 |
KF644575.1_94-137 | 12 | T | . | . | A * | . | A | . | C * | . | C * | G * | . | . | G | . | A | . | . | C | . | . | . | . | . | . | T | . | . | . | . | . | A | |
KF644574.1_T-616 | 12 | T | . | . | A * | . | A | . | C * | . | C * | G * | . | . | G | . | A | . | . | C | . | . | . | . | . | . | T | . | . | . | . | . | A | |
AP012321.1_5586 | Un1 | . | . | . | . | . | . | T * | . | A * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | 6 |
KU206432.1_Cambrid..3/1995 | Un2 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C * | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | |
KF644576.1_00c19 | Un3 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C * | . | . | . | . | . | . | . | . | . | . | |
KY852346.1_Hertf.150/2016 | Un4 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . | . | . | . | . | . | . | . | |
MF975656.1_RacL11 | Un5 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | C * | . | . | . | . | . | . | . | |
KU206462.1_Hampsh.1/2008 | Un6 | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | . | T * | . | . |
Group | 236 | 336 | 344 | 620 | 626 | 629 | 689–690 | 701 | 710 | 713 | 719 | 738–739 | 743 | 755 | 783 | 818 | 821 | 825 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
AY665713.1_Ab4 | 1 | C | C | G | C | T | G | TT | G | T | C | G | GG | C | C | G | G | G | C |
DQ172400.1_US85_1_1 | 1 | * | . | . | . | . | .. | . | . | . | . | GGG | . | . | . | . | . | . | |
DQ172310.1_AR85_1_1 | 1 | * | . | . | . | . | .. | . | . | . | . | .. | . | . | . | . | . | . | |
MH976707.1_IRL/559/2009 | 2 | . | . | . | . | . | .. | . | . | . | . | .. | . | . | . | . | . | . | |
DQ172408.1_US89_1_1 | 2 | * | . | . | . | . | . | .. | . | . | . | . | .. | T | . | . | . | . | . |
DQ172394.1_US79_1_1 | 2 | * | . | . | T | . | . | .. | . | . | . | . | .. | . | . | . | . | . | G |
DQ172309.1_AR79_1_1 | 2 | * | . | . | . | . | . | .. | . | C | . | . | .. | . | . | . | . | . | . |
DQ172384.1_US03_5_2 | 2 | * | . | . | . | . | . | .. | . | . | . | . | .. | . | . | T | . | . | . |
MH976708.1_IRL/471/2008 | 3 | . | . | . | . | . | A | .. | . | . | T | .. | . | . | . | . | . | . | |
MH976706.1_IRL/307/2015 | 3 | . | . | . | . | . | A | .. | . | . | . | T | .. | . | . | . | . | A | . |
DQ172365.1_GB89_2_1 | 3 | * | . | . | . | . | A | .. | . | A | . | T | .. | . | . | . | . | . | . |
MH976709.1_IRL/268/2001 | 4 | . | . | . | . | . | A | .. | . | . | . | . | .. | . | . | . | . | . | . |
DQ172332.1_GB00_1_1 | 4 | * | . | A | . | . | A | .. | . | . | . | . | .. | . | . | . | . | . | . |
MH976705.1_IRL/837/2007 | 5 | . | . | . | . | C | A | .. | . | G | A | . | .. | . | . | . | . | . | . |
DQ172375.1_US01_1_2 | 5 | * | . | . | . | . | A | .. | . | G | A | . | .. | . | . | . | . | . | . |
AY464052.1_V592 | 6 | . | T | . | . | . | A | .. | . | . | . | . | .. | . | T | . | . | . | . |
MH976703.1_IRL/069/1995 | 6 | . | T | . | . | . | A | .. | . | . | . | . | .. | . | T | . | . | . | . |
DQ172359.1_GB85_1_1 | 6 | * | T | . | . | . | A | .. | . | . | . | . | .. | . | T | . | . | . | . |
09m142 | . | . | . | . | . | A | .. | Start gap | - | - | - | - | - | - | - | End gap | . | . | |
MF975656.1_RacL11 | A | . | . | . | . | A | .. | Start gap | - | - | - | - | - | - | - | End gap | . | . | |
MF975655.1_KyA | A | . | . | . | . | A | GC | Start gap | - | - | - | - | - | - | - | End gap | . | . |
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Preziuso, S.; Sgorbini, M.; Marmorini, P.; Cuteri, V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes. Viruses 2019, 11, 851. https://doi.org/10.3390/v11090851
Preziuso S, Sgorbini M, Marmorini P, Cuteri V. Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes. Viruses. 2019; 11(9):851. https://doi.org/10.3390/v11090851
Chicago/Turabian StylePreziuso, Silvia, Micaela Sgorbini, Paola Marmorini, and Vincenzo Cuteri. 2019. "Equid alphaherpesvirus 1 from Italian Horses: Evaluation of the Variability of the ORF30, ORF33, ORF34 and ORF68 Genes" Viruses 11, no. 9: 851. https://doi.org/10.3390/v11090851