Journal of Functional Biomaterials

17 pages, 7758 KiB

Open AccessArticle

Comparative Efficacy of Two Hemostatic Agents in Post-Extraction Bleeding Control Following Mandibular Third Molar Surgery: A Randomized Clinical Trial

by Giovanna Pesce, Suelen Cristina Sartoretto, Rodrigo Figueiredo de Brito Resende, Madelaine Torres da Silva, Jose Mauro Granjeiro, Massimo Del Fabbro, Carlos Fernando Mourão and Monica Calasans-Maia

J. Funct. Biomater. 2025, 16(9), 305; https://doi.org/10.3390/jfb16090305 - 22 Aug 2025

Abstract

Adequate bleeding control is crucial in surgical procedures. Surgifoam and Hemospon are absorbable hemostatic sponges made from lyophilized porcine gelatin, commonly used in oral surgery. This clinical study aimed to evaluate bleeding control, soft tissue healing, and postoperative pain in dental sockets after [...] Read more.

Adequate bleeding control is crucial in surgical procedures. Surgifoam and Hemospon are absorbable hemostatic sponges made from lyophilized porcine gelatin, commonly used in oral surgery. This clinical study aimed to evaluate bleeding control, soft tissue healing, and postoperative pain in dental sockets after mandibular third molar extractions filled with Surgifoam and Hemospon. Twenty-five volunteers requiring extractions of both left and right lower third molars participated in this randomized, double-blind, split-mouth study. After extraction, each socket was randomly filled with Hemospon (test group) or Surgifoam (control group). Postoperative pain was assessed using the Visual Analog Scale (VAS) on days 1, 2, 3, and 7. Bleeding at 30 and 60 min (Souto and Mühlemann scales) and soft tissue healing at 7 and 14 days (Brancaccio scale) were evaluated using Fisher’s exact test. Bleeding scores at 30 min predominantly showed no bleeding (score 0) in 80% of participants using Surgifoam, compared to 60% in the Hemospon group. No significant differences in bleeding were observed between groups (p > 0.05), and both showed a similar reduction over time. Soft tissue healing was revealed at 14 days complete healing (score 0) in 90% of participants in both groups. No significant differences between Hemospon^® and Surgifoam^® were observed (p > 0.05). Postoperative pain evaluation showed highly variable scores on the first day (median: 2; range: 1–6) for both Surgifoam^® and Hemospon^®. By the seventh day, pain levels significantly reduced (median: 0), with no significant differences observed between the groups at any time point (p > 0.05). In conclusion, the results of this study suggest that Hemospon and Surgifoam are equally effective in bleeding control, healing, and pain control after third molar surgery. This research aims to guide surgeons on the clinical aspects of using these specific hemostatic sponges in post-extraction procedures for posterior molars and seeks to direct future clinical studies involving these materials. Full article

(This article belongs to the Special Issue Application of Biomaterials in Tissue Engineering and Regenerative Medicine)

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16 pages, 7082 KiB

Open AccessArticle

Ultrasound-Responsive Drug Delivery System Based on Piezoelectric Catalytic Mechanisms

by Kaixi Cui, Tianzheng Li, Yifei Ma, Chuanjin Zhang, Ke Zhang, Chao Qi and Kaiyong Cai

J. Funct. Biomater. 2025, 16(8), 304; https://doi.org/10.3390/jfb16080304 - 21 Aug 2025

Abstract

Ultrasound-responsive nanomaterials represent a promising approach for achieving non-invasive and localized drug delivery within tumor microenvironments. In this study, we developed a piezocatalysis-assisted hydrogel system that integrates reactive oxygen species (ROS) generation with stimulus-responsive drug release. The platform combines piezoelectric barium titanate (BTO) [...] Read more.

Ultrasound-responsive nanomaterials represent a promising approach for achieving non-invasive and localized drug delivery within tumor microenvironments. In this study, we developed a piezocatalysis-assisted hydrogel system that integrates reactive oxygen species (ROS) generation with stimulus-responsive drug release. The platform combines piezoelectric barium titanate (BTO) nanoparticles with a ROS-sensitive hydrogel matrix, forming an ultrasound-activated dual-function therapeutic system. Upon ultrasound irradiation, the BTO nanoparticles generate ROS—predominantly hydroxyl radicals (^•OH) and singlet oxygen (¹O₂)—through the piezoelectric effect, which triggers hydrogel degradation and facilitates the controlled release of encapsulated therapeutic agents. The composition and kinetics of ROS generation were evaluated using radical scavenging assays and fluorescence probe techniques, while the drug release behavior was validated under simulated oxidative environments and acoustic fields. Structural and compositional characterizations (TEM, XRD, and XPS) confirmed the quality and stability of the nanoparticles, and cytocompatibility was assessed using 3T3 fibroblasts. This synergistic strategy, combining piezocatalytic ROS generation with hydrogel disintegration, demonstrates a feasible approach for designing responsive nanoplatforms in ultrasound-mediated drug delivery systems. Full article

(This article belongs to the Special Issue Biomaterials for Drug Delivery and Cancer Therapy)

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24 pages, 4238 KiB

Open AccessArticle

Hydrothermal Magnesium Alloy Extracts Modulate MicroRNA Expression in RAW264.7 Cells: Implications for Bone Remodeling

by Viviana Costa, Lavinia Raimondi, Daniele Bellavia, Angela De Luca, Pasquale Guglielmi, Angela Cusanno, Luca Cattini, Lia Pulsatelli, Matteo Pavarini, Roberto Chiesa and Gianluca Giavaresi

J. Funct. Biomater. 2025, 16(8), 303; https://doi.org/10.3390/jfb16080303 - 21 Aug 2025

Abstract

Magnesium (Mg) alloys, particularly Mg AZ31, have emerged as promising biomaterials for orthopedic applications due to their biodegradability and favorable mechanical characteristics. Among these, the Mg AZ31+SPF alloy, subjected to hydrothermal (HT) treatment, has demonstrated enhanced bioactivity. Our previous research established that this [...] Read more.

Magnesium (Mg) alloys, particularly Mg AZ31, have emerged as promising biomaterials for orthopedic applications due to their biodegradability and favorable mechanical characteristics. Among these, the Mg AZ31+SPF alloy, subjected to hydrothermal (HT) treatment, has demonstrated enhanced bioactivity. Our previous research established that this surface modification supports the osteogenic differentiation of human mesenchymal stem cells (hMSCs) by modulating both canonical and non-canonical signaling pathways, including those implicated in osteogenesis, hypoxic response, exosome biogenesis, and lipid metabolism. In the present study, we extended our investigation to assess the effects of Mg AZ31+SPF+HT and Mg AZ31+SPF extracts on murine pre-osteoclasts (RAW 264.7 cells) over 3- and 6-day treatment periods. The primary objectives were to evaluate biocompatibility and to investigate potential impacts on osteoclastogenesis induction and miRNA expression profiles. Methods: To assess cytocompatibility, metabolic activity, DNA integrity, and morphological alterations in RAW 264.7 cells were evaluated. Osteoclast differentiation was quantified using TRAP staining, alongside the assessment of osteoclastogenic marker expression by qRT-PCR and ELISA. The immunomodulatory properties of the extracts were examined using multiplex BioPlex assays to quantify soluble factors involved in bone healing. Additionally, global miRNA expression profiling was performed using a specialized panel targeting 82 microRNAs implicated in bone remodeling and inflammatory signaling. Results: Mg AZ31+SPF+HT extract exhibited high biocompatibility, with no observable adverse effects on cell viability. Notably, a significant reduction in the number of TRAP-positive and multinucleated cells was observed relative to the Mg AZ31+SPF group. This effect was corroborated by the downregulation of osteoclast-specific gene expression and decreased MMP9 protein levels. Cytokine profiling indicated that Mg AZ31+SPF+HT extract promoted an earlier release of key cytokines involved in maintaining the balance between bone formation and resorption, suggesting a beneficial role in bone healing. Furthermore, miRNA profiling revealed a distinct regulatory signature in Mg AZ31+SPF+HT-treated cells, with differentially expressed miRNAs associated with inflammation, osteoclast differentiation, apoptosis, bone resorption, hypoxic response, and metabolic processes compared to Mg AZ31+SPF-treated cells. Conclusions: Collectively, these findings indicate that hydrothermal treatment of Mg AZ31+SPF (resulting in Mg AZ31+SPF+HT) attenuates pre-osteoclast activation by influencing cellular morphology, gene and protein expression, as well as post-transcriptional regulation via modulation of miRNAs. The preliminary identification of miRNAs and the activation of their regulatory networks in pre-osteoclasts exposed to hydrothermally treated Mg alloy are described herein. In the context of orthopedic surgery—where balanced bone remodeling is imperative—our results emphasize the dual significance of promoting bone formation while modulating bone resorption to achieve optimal implant integration and ensure long-term bone health. Full article

(This article belongs to the Special Issue Metals and Alloys for Biomedical Applications (2nd Edition))

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17 pages, 2755 KiB

Open AccessArticle

Enhanced Osteogenic Response to an Osteochondral Scaffold Modified with BMP-2 or Strontium-Enriched Amorphous Calcium Phosphate in a Co-Culture In Vitro Model

by Stefania Pagani, Manuela Salerno, Janis Locs, Jana Vecstaudza, Laura Dolcini, Milena Fini, Gianluca Giavaresi, Giuseppe Filardo and Marta Columbaro

J. Funct. Biomater. 2025, 16(8), 302; https://doi.org/10.3390/jfb16080302 - 21 Aug 2025

Abstract

Background: A trilayered collagen/collagen–magnesium–hydroxyapatite (Col/Col-Mg-HA) scaffold is used in clinical practice to treat osteochondral lesions, but the regeneration of the subchondral bone is still not satisfactory. Objective: The aim of this study was to test, in vitro, the osteoinductivity induced by [...] Read more.

Background: A trilayered collagen/collagen–magnesium–hydroxyapatite (Col/Col-Mg-HA) scaffold is used in clinical practice to treat osteochondral lesions, but the regeneration of the subchondral bone is still not satisfactory. Objective: The aim of this study was to test, in vitro, the osteoinductivity induced by the addition of bone morphogenetic protein-2 (BMP-2) or amorphous calcium phosphate granules with strontium ions (Sr-ACP), in order to improve the clinical regeneration of subchondral bone, still incomplete. Methodology: Normal human osteoblasts (NHOsts) were seeded on the scaffolds and grown for 14 days in the presence of human osteoclasts and conditioned medium of human endothelial cells. NHOst adhesion and morphology were observed with transmission electron microscopy, and metabolic activity was tested by Alamar blue assay. The expression of osteoblast- and osteoclast-typical markers was evaluated by RT-PCR on scaffolds modified by enrichment with BPM-2 or Sr-ACP, as well as on unmodified material used as a control. Results: NHOsts adhered well to all types of scaffolds, maintained their typical morphology, and secreted abundant extracellular matrix. On the modified materials, COL1A1, SPARC, SPP1, and BGLAP were more expressed than on the unmodified ones, showing the highest expression in the presence of BMP-2. On Sr-ACP-enriched scaffolds, NHOsts had a lower proliferation rate and a lower expression of RUNX2, SP7, and ALPL compared to the other materials. The modified scaffolds, particularly the one containing Sr-ACP, increased the expression of the osteoclasts’ typical markers and decreased the OPG/RANKL ratio. Both types of scaffold modification were able to increase the osteoinductivity with respect to the original scaffold used in clinical practice. BMP-2 modification seemed to be more slightly oriented to sustain NHOst activity, and Sr-ACP seemed to be more slightly oriented to sustain the osteoclast activity. These could provide a concerted action toward better regeneration of the entire osteochondral unit. Full article

(This article belongs to the Special Issue Application of Biomaterials in Tissue Engineering and Regenerative Medicine)

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14 pages, 931 KiB

Open AccessArticle

Comparative in Vitro Evaluation of Marginal Sealing in Class I Composite Restorations Using Fifth- and Seventh-Generation Adhesives

by Serban Talpos Niculescu, Ioana Veja, George-Dumitru Constantin, Ioana Elena Lile, Christos Armeniakos, Ioana Roxana Munteanu and Tareq Hajaj

J. Funct. Biomater. 2025, 16(8), 301; https://doi.org/10.3390/jfb16080301 - 20 Aug 2025

Abstract

Background: A major challenge in adhesive dentistry, often leading to restoration failure, is microleakage. This in vitro comparative study was designed to assess microleakage at the tooth–composite interface. The investigation aimed to compare the sealing efficacy of two commonly used adhesive systems. Methods: [...] Read more.

Background: A major challenge in adhesive dentistry, often leading to restoration failure, is microleakage. This in vitro comparative study was designed to assess microleakage at the tooth–composite interface. The investigation aimed to compare the sealing efficacy of two commonly used adhesive systems. Methods: Standardized Class I cavities were prepared on 20 extracted human molars and randomly divided into two groups (n = 10 each). Group A was treated with a fifth-generation total-etch adhesive (OptiBond™ Solo Plus, Kerr Corporation, Orange, CA, USA), and Group B received a seventh-generation self-etch adhesive (Adhese® Universal VivaPen®, Ivoclar Vivadent AG, Schaan, Liechtenstein). All restorations were completed using Herculite XRV composite resin. Microleakage was evaluated using dye penetration analysis after immersion in 2% methylene blue for 10 days, followed by longitudinal sectioning and microscopic measurement at 500× magnification. Results: The fifth-generation adhesive group showed a mean microleakage of 0.2503 ± 0.1921 mm, while the seventh-generation group recorded 0.2484 ± 0.1764 mm. Statistical analysis using an independent t-test revealed no significant difference between the groups (p = 0.696). Conclusions: Both adhesive systems demonstrated comparable performance in minimizing microleakage under standardized conditions. Although the total-etch group exhibited slightly lower numerical values, the difference was not statistically significant. These findings suggest that both adhesive approaches can be clinically effective when applied appropriately. Full article

(This article belongs to the Special Issue Dental Composite Resin: Characteristics and Future Perspectives)

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23 pages, 4373 KiB

Open AccessArticle

Effect of Zinc and Magnesium Compounds and Nano-Hydroxyapatite on the Physicochemical Properties and Biological Activity of Alginate and Gelatin Scaffolds for Osteochondral Defects

by Anna Morawska-Chochół, Agnieszka Urbaś, Witold Reczyński, Ewelina Kwiecień and Magdalena Rzewuska

J. Funct. Biomater. 2025, 16(8), 300; https://doi.org/10.3390/jfb16080300 - 19 Aug 2025

Abstract

Composite scaffolds based on a hydrogel matrix modified with hydroxyapatite, magnesium, or zinc compounds are promising for filling and regenerating osteochondral defects due to the specific biological properties of these modifiers. The aim of this work was to evaluate the influence of hydroxyapatite, [...] Read more.

Composite scaffolds based on a hydrogel matrix modified with hydroxyapatite, magnesium, or zinc compounds are promising for filling and regenerating osteochondral defects due to the specific biological properties of these modifiers. The aim of this work was to evaluate the influence of hydroxyapatite, nano-hydroxyapatite, magnesium chloride, and zinc oxide on mechanical properties, swelling ability, behavior in a simulated biological environment (ion release, stability, bioactivity), and antibacterial effects. Furthermore, the influence of the hydrogel matrix (alginate, gelatin, alginate/gelatin) on the selected properties was also assessed. The results showed that the addition of ZnO improved the mechanical properties of all types of matrices most effectively. Additionally, zinc ions were gradually released into the environment and partially incorporated into the formed apatite. The released zinc ions increased the inhibition zones of Staphylococcus aureus growth; however, this effect was observed only in scaffolds with an alginate matrix. This indicates that hydrogel plays a key role in antibacterial effects, beyond the contribution of antibacterial additives. No effect of magnesium on bacterial growth inhibition was observed despite its rapid release. Magnesium ions promoted efficient secretion of apatite during incubation, although it was not stable. The addition of nano-HAP significantly increased the stability of the apatite precipitates. Full article

(This article belongs to the Special Issue Multifunctional Bio-Scaffolds for Cell Growth and Tissue Morphogenesis)

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12 pages, 1363 KiB

Open AccessArticle

Antibacterial Characteristics of Nanoclay-Infused Cavit Temporary Filling Material: In Vitro Study

by Bahareh Nazemi Salman, Ayda Notash, Ali Ramazani, Shaghayegh Niaz, Seyed Mohammadrasoul Naeimi, Shayan Darvish and Ionut Luchian

J. Funct. Biomater. 2025, 16(8), 299; https://doi.org/10.3390/jfb16080299 - 19 Aug 2025

Abstract

In pediatric endodontic procedures, final crown placement is often delayed, requiring the use of temporary filling materials to seal the access cavity. Given the importance of antibacterial properties in temporary restorations and the known antimicrobial effects of nanoclay particles, this study aimed to [...] Read more.

In pediatric endodontic procedures, final crown placement is often delayed, requiring the use of temporary filling materials to seal the access cavity. Given the importance of antibacterial properties in temporary restorations and the known antimicrobial effects of nanoclay particles, this study aimed to evaluate the antibacterial efficacy of a nanoclay-infused temporary dressing against cariogenic and residual intracanal bacteria. A commercial temporary material (CAVISOL, Tehran, Iran) was blended with nanoclay (SOUTHERN, Gonzalez, TX, USA; average size 95 nm), using eugenol as a wetting agent. The antibacterial effects on Streptococcus mutans, Enterococcus faecalis, and Escherichia coli were assessed using disc diffusion, well diffusion, and microtiter plate assays after 24 h of incubation at 37 °C (six material groups, three bacterial strains, three replications for each antibacterial test). Statistical analyses were performed using Shapiro–Wilk and ANOVA tests (p < 0.05). The results showed that formulations containing 60% and 80% nanoclay significantly inhibited the growth of all tested bacteria, outperforming pure Cavit (p < 0.05). The most substantial inhibition was observed in E. coli, while S. mutans exhibited the least susceptibility (p < 0.05). These findings suggest that incorporating nanoclay into temporary fillings may enhance efficacy to increase the success rate of pediatric endodontic treatments, although further physicochemical and clinical evaluations are warranted. Full article

(This article belongs to the Special Issue Biomaterials Applied in Dental Sciences)

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3 pages, 144 KiB

Open AccessEditorial

Functional Biomaterials for Regenerative Dentistry

by Nicholas G. Fischer

J. Funct. Biomater. 2025, 16(8), 298; https://doi.org/10.3390/jfb16080298 - 19 Aug 2025

Abstract

Regenerative dentistry hopes to restore oral health by replacing diseased or damaged tissues with biologically functional, integrated counterparts [...] Full article

(This article belongs to the Special Issue Functional Biomaterials for Regenerative Dentistry)

22 pages, 1661 KiB

Open AccessArticle

Biliary Injuries Repair Using Copolymeric Scaffold: A Systematic Review and In Vivo Experimental Study

by Salvatore Buscemi, Giulia Bonventre, Andrea Gottardo, Mariano Licciardi, Fabio Salvatore Palumbo, Giovanni Cassata, Luca Cicero, Giulia Lo Monte, Roberto Puleio and Attilio Ignazio Lo Monte

J. Funct. Biomater. 2025, 16(8), 297; https://doi.org/10.3390/jfb16080297 - 18 Aug 2025

Abstract

Background: Common bile duct (CBD) treatments are often associated with complications, limiting long-term efficacy. To overcome these issues, polymeric grafts have been suggested as promising alternatives, since they are highly customizable, biocompatible, and may reduce side effects frequency. Methods: A systematic review was [...] Read more.

Background: Common bile duct (CBD) treatments are often associated with complications, limiting long-term efficacy. To overcome these issues, polymeric grafts have been suggested as promising alternatives, since they are highly customizable, biocompatible, and may reduce side effects frequency. Methods: A systematic review was conducted, interrogating MEDLINE and Cochrane Library. Next, an in vivo study involved 20 pigs, which underwent a former controlled biliary injury. To repair the defect, a α,β-Poly(N-2-hydroxyethyl)-DL-Aspartamide (PHEA)–Polylactic-acid (PLA)–Polycaprolactone (PCL) scaffold was implanted. The animals were sacrificed at one and three months for gross and histological examinations, to assess tissue integration and healing outcomes. Results: The systematic review highlighted that such scaffolds have shown promising results in CBD regeneration, both in single and joined applications. These findings were confirmed by the in vivo study, where the use of such scaffolds—particularly, the planar ones—led to safe and complete bile duct regeneration. Histological analysis revealed lymphomonocytic infiltrates and neovascularization, while microscopic examination showed progressive scaffold degradation accompanied by biliary tissue regeneration. Conclusions: Experimental results are consistent with the literature, confirming the potential of such polymeric scaffolds in aiding complete CBD regeneration and being reabsorbed shortly after. Still, further studies are needed to fully validate their translational application. PROSPERO ID: CRD420251115056. Full article

(This article belongs to the Special Issue Polymers Materials Used in Biomedical Engineering)

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18 pages, 2248 KiB

Open AccessArticle

Influence of Drilling Protocol on Primary Implant Stability Depending on Different Bone Qualities and Implant Macro-Designs, Lengths, and Diameters

by Milan Stoilov, Ramin Shafaghi, Lea Stoilov, Helmut Stark, Michael Marder, Norbert Enkling and Dominik Kraus

J. Funct. Biomater. 2025, 16(8), 296; https://doi.org/10.3390/jfb16080296 - 16 Aug 2025

Abstract

Background: Primary implant stability is a critical factor for successful osseointegration and long-term implant success. This study investigates the impact of drilling protocol modifications on primary stability, considering different bone qualities and implant macro-designs, lengths, and diameters. Material and Methods: Three implant designs—two [...] Read more.

Background: Primary implant stability is a critical factor for successful osseointegration and long-term implant success. This study investigates the impact of drilling protocol modifications on primary stability, considering different bone qualities and implant macro-designs, lengths, and diameters. Material and Methods: Three implant designs—two parallel-walled and one tapered—were tested with diameters ranging from 3.4 to 5.2 mm and lengths from 7.5 to 14.5 mm. Implants were placed in polyurethane foam blocks simulating different bone densities (10, 15, 25, and 35 PCF). A standard drilling protocol was used in all groups, with modifications based on bone quality: overpreparation in dense bone and underpreparation in softer bone. Primary stability was evaluated using insertion torque (IT). The optimal IT range was defined as 25–50 Ncm, based on clinical guidelines for immediate loading. The influence of drilling protocol adaptations on stability parameters was assessed. Results: Insertion torque was primarily influenced by bone density and implant diameter, with implant length playing a minor role. In dense bone (D1, D2), underpreparation improved torque values, especially in smaller implants, while overpreparation reduced them. The highest torques occurred with 5.2 mm implants, sometimes exceeding 80 Ncm. Standard protocols did not consistently achieve optimal torque across implant types. In soft bone (D3), underpreparation—particularly with tapered implants—was modestly beneficial. In very soft bone (D4), none of the protocols reliably reached the desired torque range. Conclusions: Adapting drilling protocols to bone density improves insertion torque, especially with wider implants and in denser bone. Underpreparation is generally more effective than overpreparation. However, in very soft bone, neither implant geometry nor drilling adaptations reliably achieve optimal primary stability, highlighting the need for additional strategies. Full article

(This article belongs to the Section Dental Biomaterials)

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21 pages, 3052 KiB

Open AccessArticle

MIL-53 MOF on Sustainable Biomaterial for Antimicrobial Evaluation Against E. coli and S. aureus Bacteria by Efficient Release of Penicillin G

by Delia Monserrat Ávila-Márquez, Alien Blanco Flores, Helen Paola Toledo Jaldin, Mateo Burke Irazoque, Maribel González Torres, Alfredo Rafael Vilchis-Nestor, Carla Calderon Toledo, Sergio Gutiérrez-Cortez, Juan Pablo Díaz Rodríguez and Alejandro Dorazco-González

J. Funct. Biomater. 2025, 16(8), 295; https://doi.org/10.3390/jfb16080295 - 15 Aug 2025

Abstract

The development of efficient antibiotic-releasing materials derived from sustainable and recyclable compounds represents a key area within biomedical materials science, particularly in the treatment of antibacterial infections. Herein, a Fe³⁺/terephthalate-based metal–organic framework (MIL-53) and a novel advanced material made [...] Read more.

The development of efficient antibiotic-releasing materials derived from sustainable and recyclable compounds represents a key area within biomedical materials science, particularly in the treatment of antibacterial infections. Herein, a Fe³⁺/terephthalate-based metal–organic framework (MIL-53) and a novel advanced material made of MIL-53 with biogenic hydroxyapatite (1) were prepared by solvothermal reactions, and these were studied in detail as a Penicillin-G-releasing material. After loading Penicillin G on 1 and MIL-53, the antibiotic percentage release was studied, and the antimicrobial effectiveness of each material was evaluated against two bacterial ATCC strains (E. coli and S. aureus) and various Penicillin-G-resistant uropathogenic strains such as E. coli isolates (HHM 25, ERV 6, and FGI 4). Functional, structural, and morphological characteristics of these materials were thoroughly studied by analytical tools (FTIR, XRD, BET, SEM-EDS, and XPS). The Penicillin G load did not exceed 50% in both materials. The Penicillin G adsorption mechanism involves several types of interactions with the materials. The release of the antibiotic was more efficient from MIL-53, where the load did not exceed 20%. The release was analyzed using mathematical models. They indicated that when Penicillin G is released from MIL-53, the process follows diffusion through a uniform matrix; however, 1 is more porous, which helps with the release by diffusion of Penicillin G, and 1 exhibits more than a 90% inhibition of the growth of bacteria and strains like MIL-53. This suggests a valuable approach to antibiotic activity against resistant pathogens. The use of composite materials derived from the Fe-MOF with a sustainable matrix of hydroxyapatite as antibiotic-releasing materials has been unexplored until now. Full article

(This article belongs to the Topic Recent Advances in Composite Biomaterials)

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14 pages, 4450 KiB

Open AccessArticle

Photothermally Responsive Biomimetic Composite Scaffolds Based on Polydopamine-Functionalized Nanoparticles/Polyurethane for Bone Repair

by Ruqing Bai, Jiaqi Chen, Ting Zhang, Tao Chen, Xiaoying Liu, Weihu Yang, Tuck-Whye Wong, Jianwei Zhang and Li Wang

J. Funct. Biomater. 2025, 16(8), 294; https://doi.org/10.3390/jfb16080294 - 15 Aug 2025

Abstract

In this study, a shape-changeable 3D scaffold with photothermal effects was developed to address the clinical challenges of complex bone defects. The multifunctional construct was fabricated via in situ polymerization combined with a gas foaming technique, creating hierarchical porous architectures that mimic the [...] Read more.

In this study, a shape-changeable 3D scaffold with photothermal effects was developed to address the clinical challenges of complex bone defects. The multifunctional construct was fabricated via in situ polymerization combined with a gas foaming technique, creating hierarchical porous architectures that mimic the native bone extracellular matrix. By incorporating polydopamine (PDA)-modified amorphous calcium phosphate (CA) into poly(propylene glycol) (PPG)- and poly(ԑ-caprolactone) (PCL)-based polyurethane (PU). The obtained scaffolds achieved osteoinductive potential for bone tissue engineering. The surface PDA modification of CA enabled efficient photothermal shape conversion under near-infrared (NIR) irradiation, facilitating non-invasive remote control of localized hyperthermia. The optimized scaffolds exhibited interconnected porosity (approximately 70%) with osteoconductive pore channels (200–500 μm), resulting in good osteoinduction in cell culture, and precise shape-memory recovery at physiological temperatures (~40 °C) under NIR for minimally invasive delivery. The synergistic effect of osteogenesis promotion and photothermal transition demonstrated this programmable scaffold as a promising solution for integrated minimally invasive bone repair and defect reconstruction. Full article

(This article belongs to the Section Biomaterials for Tissue Engineering and Regenerative Medicine)

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36 pages, 775 KiB

Open AccessSystematic Review

Efficacy of Endodontic Files in Root Canal Retreatment: A Systematic Review of In Vitro Studies

by Anna Soler-Doria, José Luis Sanz, Marcello Maddalone and Leopoldo Forner

J. Funct. Biomater. 2025, 16(8), 293; https://doi.org/10.3390/jfb16080293 - 14 Aug 2025

Abstract

The success rate of root canal treatment is high, but it can fail. In these cases, orthograde root canal retreatment is often the treatment of choice, for which numerous biomaterials are available on the market, including endodontic files. This systematic review aimed to [...] Read more.

The success rate of root canal treatment is high, but it can fail. In these cases, orthograde root canal retreatment is often the treatment of choice, for which numerous biomaterials are available on the market, including endodontic files. This systematic review aimed to study the endodontic files available on the market and establish their efficacy in root canal retreatment. An electronic search of six different databases was performed, and in vitro experimental studies that studied root canal cleaning, debris extrusion, retreatment time, or dentinal damage produced with any of the comparison methods were selected. The quality of the studies was assessed with the QUIN scale. In total, 78 studies were included for the analysis, of which 9 had a high risk of bias, 53 had a moderate risk, and 16 had a low risk. The methods used to evaluate the efficacy of endodontic files in root canal retreatment are heterogeneous. Manual files produce more apical extrusion than rotary files. PTUR files are the most studied endodontic files. It is the file system that leaves the least residual filling material in the canal, takes the least time during retreatment, and removes the greatest amount of dentine. However, no file system achieved the complete removal of the root canal filling material. No consistent pattern emerged across studies when comparing rotary files with continuous and reciprocating rotation in terms of the removal of the filling material, the time required for de-obturation, dentine damage produced, or apical extrusion. Full article

(This article belongs to the Special Issue The 15th Anniversary of JFB—Endodontic Biomaterials)

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15 pages, 1514 KiB

Open AccessArticle

Injectable Thermosensitive Hydrogel Containing Bakuchiol Reduces Periodontal Inflammation and Alveolar Bone Loss in a Rat Model

by Seong-Jin Shin, Gyu-Yeon Shim, Seong-Hee Moon, Yu-Jin Kim, Hyun-Jin Kim, Seunghan Oh, Jung-Hwan Lee and Ji-Myung Bae

J. Funct. Biomater. 2025, 16(8), 292; https://doi.org/10.3390/jfb16080292 - 13 Aug 2025

Abstract

This study aimed to develop and evaluate a bakuchiol-loaded thermosensitive hydrogel (BTH) as a novel local drug delivery system for the management of periodontitis. Bakuchiol, a natural phenolic compound extracted from Psoralea corylifolia, was incorporated into a hydrogel composed of poloxamers and [...] Read more.

This study aimed to develop and evaluate a bakuchiol-loaded thermosensitive hydrogel (BTH) as a novel local drug delivery system for the management of periodontitis. Bakuchiol, a natural phenolic compound extracted from Psoralea corylifolia, was incorporated into a hydrogel composed of poloxamers and carboxymethylcellulose. The gelation behavior, physicochemical properties, and drug release profile were analyzed. Additionally, antibacterial activity against Porphyromonas gingivalis was assessed. Cytotoxicity was evaluated in human gingival fibroblasts and RAW 264.7 cells. Anti-inflammatory effects were determined by measuring proinflammatory cytokine expression in lipopolysaccharide-stimulated RAW 264.7 macrophages. Furthermore, alveolar bone loss, cytokine expression, and histological findings were assessed in a rat model of ligature-induced periodontitis. BTH demonstrated sol–gel transition at body temperature, with sustained drug release over 15 days. Moreover, it exhibited significant antibacterial activity against P. gingivalis and was non-cytotoxic at an extract concentration of 6.25%. In vitro, it significantly downregulated inflammatory cytokines in activated macrophages. In vivo, BTH application reduced alveolar bone loss and interleukin-1β expression in gingival tissues. Histological analysis confirmed decreased inflammatory cell infiltration and alveolar bone destruction. Thus, BTH demonstrated both antibacterial and anti-inflammatory activities, exhibiting potential as a promising therapeutic strategy for localized periodontal treatment. Full article

(This article belongs to the Section Dental Biomaterials)

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19 pages, 4137 KiB

Open AccessArticle

Analysis of Insertion Torque of Orthodontic Mini-Implants Depending on the System and the Morphological Substrate

by Tamara Rahela Ioana, Filip George Boeru, Ioana Mitruț, Anne-Marie Rauten, Mahmoud Elsaafin, Mihaela Ionescu, Ionela Elisabeta Staicu and Horia Octavian Manolea

J. Funct. Biomater. 2025, 16(8), 291; https://doi.org/10.3390/jfb16080291 - 13 Aug 2025

Abstract

Orthodontic mini-implants are well-known anchorage devices and stand out as a particularly effective tool for ensuring maximum anchorage without relying on patient compliance. Therefore, it is necessary to understand what levels of torque strains remain in the physiological limits and can guarantee the [...] Read more.

Orthodontic mini-implants are well-known anchorage devices and stand out as a particularly effective tool for ensuring maximum anchorage without relying on patient compliance. Therefore, it is necessary to understand what levels of torque strains remain in the physiological limits and can guarantee the stability of these mini-implants. The aim of this study was to investigate and measure the initial and final torque values of orthodontic mini-implants when placed perpendicular to the maxillary and mandibular bone surfaces. In our study, orthodontic mini-implants from different companies were inserted perpendicularly using different insertion torques on the plate of both maxillary and mandibular bones from pig specimens. The torque values were then analyzed. The results of this study highlight the need for continued research to analyze the ideal insertion torque of different types of mini-implants depending on the insertion area, in order to achieve clinical success of mini-implants. Full article

(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry)

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14 pages, 3562 KiB

Open AccessArticle

Relationship Between Fracture Toughness and Fracture Mirror in Modern Polymer-Based Dental Composites

by Nicoleta Ilie

J. Funct. Biomater. 2025, 16(8), 290; https://doi.org/10.3390/jfb16080290 - 12 Aug 2025

Abstract

The mechanical behavior of dental composites depends on the sample size and stress configuration. This makes it difficult to extrapolate laboratory data to clinical restorations with significant variations in size and geometry. Intrinsic parameters, such as fracture toughness, are therefore of great importance, [...] Read more.

The mechanical behavior of dental composites depends on the sample size and stress configuration. This makes it difficult to extrapolate laboratory data to clinical restorations with significant variations in size and geometry. Intrinsic parameters, such as fracture toughness, are therefore of great importance, even if they are less common and more difficult to measure. The aim of this study was to apply principles of fractography and fracture mechanics to exploit the results obtained from a three-point bending test. The objectives include calculating a material-specific constant, validating the experimental findings, and establishing a correlation with fracture toughness. Forty representative composites with wide variation in filler quantity (65–83% by weight and 46.4–64% by volume), type (compact glasses and pre-polymerized), and composition were examined. Fracture toughness/K_Ic was evaluated in a notchless triangular prism test. Fracture type, origin, and mirror size were determined on 280 flexural fracture specimens (n = 20). The amount of filler strongly influences all measured parameters, with the effect strength varying in the sequence: mechanical work (η_P² = 0.995), modulus of elasticity (η_P² = 0.991), flexural strength (η_P² = 0.988), fracture toughness (η_P² = 0.979), and mirror constant (η_P² = 0.965). Fracture surfaces allowed the delineation of the fracture mirror and the application of fracture mechanics approaches. The mirror constant was derived from the radius of the fracture mirror, measured in the direction of constant stress, using Orr’s equation, and correlates well with K_Ic (0.81). Larger confidence intervals were observed for the mirror constant data, while for 5 of 14 materials, the mirror constant was overestimated compared to K_Ic. The overestimation was attributed to the lower refractive index of the urethane methacrylate composition. Full article

(This article belongs to the Section Dental Biomaterials)

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15 pages, 1434 KiB

Open AccessArticle

Shear Bond Strength of Self-Adhesive and Self-Etching Resin Cements to Dentin for Indirect Restorations

by Janet Kirilova, Georgi Veselinov Iliev, Sevda Yantcheva, Elitsa Deliverska and Viktoria Petrova

J. Funct. Biomater. 2025, 16(8), 289; https://doi.org/10.3390/jfb16080289 - 12 Aug 2025

Abstract

This study assessed and compared the shear bond strength of self-adhesive and self-etching resin cements for indirect aesthetic restorations to dentin. Four different materials, lithium disilicate ceramics, zirconia ceramics, polymethyl methacrylate (PMMA) composites, and hybrid materials, were used for indirect restorations cemented to [...] Read more.

This study assessed and compared the shear bond strength of self-adhesive and self-etching resin cements for indirect aesthetic restorations to dentin. Four different materials, lithium disilicate ceramics, zirconia ceramics, polymethyl methacrylate (PMMA) composites, and hybrid materials, were used for indirect restorations cemented to dentin. The null hypothesis was that there would be no differences in shear bond strength between the investigated materials. Eighty extracted human molars were used. Eighty dentin specimens with a flat surface were prepared and randomly distributed in groups of 10 (n = 10). From each material (Cerasmart 270, Initial LiSi Blok, Katana ZR Noritake, and Crowntec Next Dent), 20 blocks were made and cemented to the dentin samples. Half of the blocks from each material were cemented to dentin using self-etching resin cement (Panavia V5), and the other half using self-adhesive resin cement (i-CEM). After the specimens were prepared, a laboratory test was conducted to evaluate the shear bond strength. The fracture type was determined using a light microscope, and SEM confirmed the results. The results were statistically analysed. All materials cemented with self-etching cements (Panavia V5) showed statistically higher shear strength values than those cemented with self-adhesive resin cement (i-CEM). In the specimen groups where self-adhesive cement (i-CEM) was used, Cerasmart 270 bonded statistically better. A statistical difference was found between all groups of materials cemented with self-etching cement. The Initial LiSi Block showed the strongest bond, followed by Katana Zr Noritake, Crowntec NextDent, and Cerasmart 270. Adhesion fracture to dentin was observed for all groups cemented with i-CEM. This study highlights the superior performance of self-etching cements in terms of shear bond strength. 10-Methacryloyloxydecyl dihydrogen phosphate (10-MDP), a functional monomer, was found to enhance adhesion strength significantly. However, using self-adhesive cements was associated with a weaker bond to dentin, highlighting the importance of the right cementing agent in restorative dentistry. Full article

(This article belongs to the Special Issue Biomaterials for Dental Reparative and Regenerative Therapies)

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13 pages, 3998 KiB

Open AccessArticle

Promoting Surface Energy and Osteoblast Viability on Zirconia Implant Abutments Through Glass–Ceramic Spray Deposition Technology

by Wen-Chieh Hsu, Tao-Yu Cha, Yu-Chin Yao, Chien-Ming Kang, Sheng-Han Wu, Yuichi Mine, Chien-Fu Tseng, I-Ta Lee, Dan-Jae Lin and Tzu-Yu Peng

J. Funct. Biomater. 2025, 16(8), 288; https://doi.org/10.3390/jfb16080288 - 7 Aug 2025

Abstract

Zirconia is used widely for high-precision custom abutments; however, stress concentration can compromise osseointegration. Although glass–ceramic spray deposition (GCSD) can enhance the surface properties of zirconia, its biological effects remain unclear. In this study, the biological responses of human osteoblast-like (MG-63) cells to [...] Read more.

Zirconia is used widely for high-precision custom abutments; however, stress concentration can compromise osseointegration. Although glass–ceramic spray deposition (GCSD) can enhance the surface properties of zirconia, its biological effects remain unclear. In this study, the biological responses of human osteoblast-like (MG-63) cells to GCSD-modified zirconia surfaces were evaluated to assess the potential application in zirconia abutments. Disk-shaped zirconia and titanium alloy samples were prepared; titanium served as the control (Ti). Zirconia was subjected to polishing (NT), airborne-particle abrasion (AB), or GCSD with (GE) or without (GC) hydrofluoric acid (HF) etching. Surface characteristics, including wettability, surface energy (SE), and surface potential (SP), were analyzed. Cytotoxicity and MG-63 cell adhesion were assessed using the PrestoBlue assay, scanning electron microscopy (SEM), viability staining, and confocal laser scanning microscopy (CLSM). Statistical analysis was performed with a significance level of 0.05. GCSD produced a dense glass–ceramic coating on the zirconia surface, which significantly enhanced hydrophilicity as indicated by reduced water contact angles and increased SE in the GC and GE groups (p < 0.05). HF etching increased SP (p < 0.05). No cytotoxicity was observed in any group. SEM, viability staining, and CLSM revealed enhanced MG-63 cell attachment on Ti and GE surfaces and the highest viability ratio in the GE group. The NT group exhibited the lowest cell attachment and viability at all time points. GCSD effectively improved zirconia abutment surface properties by enhancing hydrophilicity and promoting MG-63 cell adhesion, with biocompatibility comparable to or better than that of titanium. Full article

(This article belongs to the Special Issue Surface Analyses, Physicochemical and Mechanical Properties of Dental Biomaterials (2nd Edition))

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18 pages, 7273 KiB

Open AccessArticle

Functional Compression Fabrics with Dual Scar-Suppressing and Antimicrobial Properties: Microencapsulation Design and Performance Evaluation

by Lihuan Zhao, Changjing Li, Mingzhu Yuan, Rong Zhang, Xinrui Liu, Xiuwen Nie and Bowen Yan

J. Funct. Biomater. 2025, 16(8), 287; https://doi.org/10.3390/jfb16080287 - 5 Aug 2025

Abstract

Pressure therapy combined with silicone has a significant effect on scar hyperplasia, but limitations such as long-term wearing of compression garments (CGs) can easily cause bacterial infection, cleanliness, and lifespan problems of CGs caused by the tedious operation of applying silicone. In this [...] Read more.

Pressure therapy combined with silicone has a significant effect on scar hyperplasia, but limitations such as long-term wearing of compression garments (CGs) can easily cause bacterial infection, cleanliness, and lifespan problems of CGs caused by the tedious operation of applying silicone. In this study, a compression garment fabric (CGF) with both inhibition of scar hyperplasia and antibacterial function was prepared. A polydimethylsiloxane (PDMS)-loaded microcapsule (PDMS-M) was prepared with chitosan quaternary ammonium salt (HACC) and sodium alginate (SA) as wall materials and PDMS as core materials by the complex coagulation method. The PDMS-Ms were finished on CGF and modified with (3-aminopropyl)triethoxysilane (APTES) to obtain PDMS-M CGF, which was further treated with HACC to produce PDMS-M-HACC CGF. X-ray Photoelectron Spectroscopy(XPS) and Fourier transform infrared spectroscopy (FTIR) analysis confirmed the formation of covalent bonding between PDMS-M and CGF. The PDMS-M CGF exhibited antibacterial rates of 94.2% against Gram-negative bacteria Escherichia coli (E. coli, AATCC 6538) and of 83.1% against Gram-positive bacteria Staphylococcus aureus (S. aureus, AATCC 25922). The antibacterial rate of PDMS-M-HACC CGF against both E. coli and S. aureus reached 99.9%, with wash durability reaching grade AA for E. coli and approaching grade A for S. aureus. The finished CGF maintained good biocompatibility and showed minimal reduction in moisture permeability compared to unfinished CGF, though with decreased elastic recovery, air permeability and softness. The finished CGF of this study is expected to improve the therapeutic effect of hypertrophic scars and improve the quality of life of patients with hypertrophic scars. Full article

(This article belongs to the Special Issue Biomaterials, Bioconjugated Materials, and Biomaterial Composites with Antimicrobial Properties)

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Journal of Functional Biomaterials

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