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Nitrogen, Volume 7, Issue 3

2026 September - 38 articles

Cover Story: Optimizing nitrogen timing is essential for productive and sustainable durum wheat production. In a Mediterranean field experiment, six split-nitrogen strategies were evaluated using ground-based hyperspectral sensing and UAV multispectral imagery across successive growth stages. The three-way split strategy (N3: 33% at sowing, 33% at tillering, and 33% at stem elongation) produced the most favorable overall crop response, including higher leaf nutrient concentrations, stronger vegetation index responses, greater plant height, and the highest grain yield. The UAV-derived NDVI at booting showed the strongest relationship with grain yield (r = 0.717, p < 0.01). Integrating spectral and structural information provides a practical framework for phenology-based precision nitrogen management. View this paper
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Articles (38)

  • Article
  • Open Access
532 Views
29 Pages

Responses of Winter Barley Genotypes to Low Nitrogen Supply Under Rainfed Conditions

  • Liliana Vasilescu,
  • Eugen-Iulian Petcu,
  • Ioana Crișan,
  • Elena Partal and ,
  • Vasile Silviu Vasilescu,
  • Alexandrina Sîrbu,
  • Leon Muntean and
  • Andreea D. Ona
  • + 3 authors+ 0 author

16 September 2026

Reducing nitrogen inputs while maintaining grain yield and quality is an important objective for sustainable barley production. This study evaluated 39 winter barley genotypes (20 six-row and 19 two-row) under two N management treatments following pe...

  • Article
  • Open Access
462 Views
16 Pages

Trace Element Bioavailability Shapes Bacterial Communities and Biological Nitrogen Fixation in Paddy Soils

  • Azhar Sohail Shahzad,
  • Laraib Sheikh,
  • Yunfei Yu,
  • Yifan Guan and ,
  • Sohaib Mehmood and
  • Shunyao Zhuang
  • + 1 author+ 0 author

14 September 2026

Biological nitrogen fixation (BNF) is a key nitrogen source in paddy soils, yet its spatial variability remains poorly understood. In this study, we conducted a 90-day 15N2 labeling experiment using soils from seven paddy fields in Zhejiang Province,...

(This article belongs to the Special Issue Microbial Interactions with Plants: Advancing Nitrogen Fixation, Uptake, and Utilization, 2nd Edition)
  • Article
  • Open Access
347 Views
16 Pages

Nitrogen Form Modulates Plant Responses to Heterogeneous Iron Availability in Arabidopsis and Wheat

  • Xiaodi Shi,
  • Yan Li,
  • Xiumin Cui,
  • Xinhao Gao and ,
  • Guilan Duan,
  • Weiming Shi,
  • Herbert J. Kronzucker and
  • Guangjie Li
  • + 3 authors+ 0 author

10 September 2026

Plants commonly encounter spatially heterogeneous distributions of nitrogen (N) forms and iron (Fe) in soils, but how N form modifies plant responses to heterogeneous Fe availability remains poorly understood. Using a split-root system, we examined t...

  • Article
  • Open Access
505 Views
17 Pages

Interpretable Machine Learning Identifies Nitrogen Turnover Signatures in Soil Ammonia Emissions

  • Eszter Tóth,
  • Zoltán Bozóki,
  • László Horváth,
  • Helga Huszár and ,
  • Tünde Takács and
  • Anna Szabó
  • + 1 author+ 0 author

10 September 2026

Soil ammonia (NH3) emissions are a major pathway for reactive nitrogen loss, but their spatial variability is difficult to explain using equilibrium-based approaches alone. This study evaluated whether machine learning can improve the interpretation...

  • Review
  • Open Access
755 Views
25 Pages

Nanomaterials for Soybean Growth Promotion and Stress Tolerance: A Review of Mechanisms and Applications

  • Yuqi Liu,
  • Xuehong Wang,
  • Xiaojun Zhang,
  • Xuanyao Lin and ,
  • Shuming Wang,
  • Xianchun Zong and
  • Yuelei Wang
  • + 2 authors+ 0 author

8 September 2026

Soybean (Glycine max) is a globally important source of protein and oil, and its capacity for biological nitrogen fixation (BNF) underpins its strategic role in sustainable agriculture. However, BNF efficiency is highly sensitive to abiotic stresses,...

(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
  • Article
  • Open Access
371 Views
13 Pages

7 September 2026

Improving nitrogen (N) use efficiency while reducing environmental losses remains a major challenge in crop production. Zeolites, due to their high cation exchange capacity, have been proposed as carriers for ammonium-based fertilizers, potentially m...

(This article belongs to the Topic Ammonium Biology: From Molecular Response to Fertilization)
  • Article
  • Open Access
344 Views
15 Pages

Transcriptomic and Biochemical Responses of Camellia sinensis (L.) Kuntze Callus Cultures to Nitrogen Deficiency In Vitro

  • Karina A. Manakhova,
  • Maya V. Gvasaliya,
  • Lyudmila S. Malyukova,
  • Lada V. Zhokhova and ,
  • Alexey V. Ryndin and
  • Evgeny I. Rogaev
  • + 1 author+ 0 author

4 September 2026

Nitrogen deficiency is one of the key factors limiting the growth and raw-material quality of tea plants; however, cell-autonomous mechanisms underlying the response of Camellia sinensis (L.) Kuntze to reduced nitrogen availability remain insufficien...

  • Article
  • Open Access
1 Citations
728 Views
20 Pages

Yield and Grain Quality Response of Winter Wheat to Different Nitrogen and Phosphorus Fertilization Doses

  • Alexandra Leonte,
  • Sabina-Andreea Pintilie,
  • Simona Florina Isticioaia,
  • Ana-Maria Vălean and
  • Nicoleta Olimpia Vrînceanu

3 September 2026

Climate change increasingly challenges winter wheat production, making balanced nutrient management essential for maintaining crop productivity and grain quality. In this context, fertilization remains a key factor determining crop productivity and y...

  • Article
  • Open Access
395 Views
17 Pages

The Potential Inhibitory Effect of Copper and Nickel on Gaseous Soil N Emissions

  • Aránzazu Louro López,
  • Nadine Loick,
  • Saoirse Sheehy Ariff,
  • Cheng-Hsien Lin and ,
  • Mariana Rosas Alenicov and
  • Laura Maritza Cardenas
  • + 1 author+ 0 author

3 September 2026

Nickel (Ni) and copper (Cu) are essential soil micronutrients that are beneficial for plant growth and development when applied at the right levels to deficient soils. However, they can also be of concern if excessive application leads to accumulatio...

  • Article
  • Open Access
501 Views
29 Pages

Interactive Effects of Nitrogen and Iron Fertilization on Canola (Brassica napus L.) Productivity and Oil Quality Under Arid Conditions: A Maximum Yield Threshold Approach

  • Mahmoud A. Mohamed,
  • Ahmed M. Ali,
  • Hassan M. Al-Sayed,
  • Yasser A. Sayed and ,
  • Doaa M. Abou El-Fadl,
  • Omhashem L. A. Youssef,
  • Mohamed E. Fadl and
  • Antonio Scopa
  • + 3 authors+ 0 author

1 September 2026

Excessive nitrogen (N) fertilization poses environmental risks and can induce micronutrient imbalances that limit crop productivity. This two-season field study (2024/2025–2025/2026) investigated the interactive effects of four nitrogen (N) rat...

(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
  • Review
  • Open Access
1,023 Views
22 Pages

Same Nodes, Different Flux: A Unified Microbial Framework for Nitrogen in Composting and Anaerobic Digestion

  • Baber Ali,
  • Aqsa Hafeez,
  • Nijat Imin,
  • Adnan Arshad and ,
  • Ionuț Ovidiu Jerca and
  • Fatjon Cela
  • + 1 author+ 0 author

1 September 2026

Nitrogen cycling during the biological treatment of organic waste is governed by a consortium of microorganisms whose activity determines whether nitrogen is conserved as a fertiliser resource or lost as ammonia, nitrous oxide, or dinitrogen. Compost...

(This article belongs to the Special Issue Linking Microbial Processes and Nitrogen Cycling in Organic Residue Valorization)
  • Article
  • Open Access
404 Views
18 Pages

28 August 2026

To support in situ lunar regolith resource utilization for a lunar base Controlled Ecological Life Support System (CELSS), this study selected the nitrogen-fixing cyanobacterium Nostoc commune and halophyte Suaeda salsa (L.) Pall. based on extremophi...

  • Article
  • Open Access
916 Views
11 Pages

AR6-Harmonized Estimation and Crop-Specific Distribution of Nitrous Oxide Emissions from Agricultural Soils in Pakistan

  • Muhammad Aamer Maqsood,
  • Naqshe Zuhra,
  • Tariq Aziz,
  • Muhammad Zia-ur-Rehman and ,
  • Talha Usman,
  • Muhammad Aslam,
  • Abdul Majid,
  • Arif Goheer,
  • Abiola Adeyemi and
  • Muhammad Imtiaz
  • + 6 authors+ 1 author

28 August 2026

Accurate assessment of nitrous oxide (N2O) emissions from agricultural soils is essential for developing effective mitigation strategies. However, Pakistan’s current baseline estimates show inconsistencies. The updated Nationally Determined Con...

  • Article
  • Open Access
506 Views
33 Pages

Associations Between Grain Nitrogen Accumulation and Maize Yield Formation Under Foliar Fertilization—An Exploratory Multivariate Analysis

  • Zhantian Zhang,
  • Haining Chen,
  • Wei Zhou,
  • Jie Yao and ,
  • Weifeng Leng,
  • Tianjing Yang,
  • Baoyou Liu and
  • Zhaobo Fan
  • + 3 authors+ 0 author

27 August 2026

This study aimed to evaluate the comprehensive effects of foliar fertilization on maize-related traits and to identify nutrient-related factors associated with yield variation. A single-site field experiment was conducted during the 2024 growing seas...

  • Article
  • Open Access
639 Views
23 Pages

Wheat Dust as a Sustainable Soil Amendment Improves Durum Wheat Performance and Drought Resilience

  • Thouraya Ben Hammouda,
  • Wissal M’sehli,
  • Imran Hammami and
  • Darine Trabelsi

27 August 2026

Wheat dust, an underutilized agro-industrial by-product, is rich in nutrients and may serve as a sustainable soil amendment. This study evaluated its effects on wheat (Triticum durum L.) growth, nitrogen metabolism, and drought tolerance. Two greenho...

(This article belongs to the Special Issue Genomics and Molecular Ecology of Microbial Nitrogen Fixation and Ecosystem Nitrogen Cycling)
  • Article
  • Open Access
1,038 Views
20 Pages

26 August 2026

Crop rotation and fertilization are two of the most important agrotechnical measures that influence the chemical properties of the soil and its capacity to support stable agricultural production, especially in areas affected by erosion and climate ch...

  • Article
  • Open Access
742 Views
25 Pages

Effect of Nitrogen and Environment Interaction on Maize Yield Productivity

  • Nataša Ljubičić,
  • Vera Popović,
  • Marko Kostić,
  • Nevena Stevanović and ,
  • Maša Buđen,
  • Nikola Stanković,
  • Tijana Barošević and
  • Aleksandar Ivezić
  • + 3 authors+ 0 author

19 August 2026

Variation in maize performance under different nitrogen fertilization regimes depends on genotype response, environmental conditions, and their interaction. This study evaluated three commercial maize hybrids contrasting maturity groups (G1–P95...

  • Article
  • Open Access
1 Citations
850 Views
21 Pages

Synergistic Effects of Microbial Inoculation and Nitrogen Fertilization on Maize Performance and Yield Attributes

  • Sajjad Hussain,
  • Saeed Ahmad Qaisrani,
  • Muhammad Mubeen,
  • Hafiz Muhammad Rashad Javeed and
  • Muhammad Tahir

18 August 2026

Partial replacement of chemical fertilizers, particularly nitrogen fertilizers, with biological fertilizers is considered an effective strategy for reducing the negative environmental impacts associated with excessive fertilizer use. This study aimed...

(This article belongs to the Special Issue Effects of Nitrogen Fertilizer Management and Microbial Inoculation on Crops)
  • Article
  • Open Access
639 Views
22 Pages

14 August 2026

Nitrogen (N) is one of the most important nutrients influencing wheat growth, plant nutrition, and grain production. Appropriate timing of nitrogen application is essential to synchronize nutrient availability with crop demand. This study evaluated s...

(This article belongs to the Special Issue Optimizing Water and Nitrogen Management for Sustainable Crop Production and Greenhouse Gas Mitigation)
  • Article
  • Open Access
503 Views
10 Pages

Effect of Liquid Humalite on Symbiotic Nitrogen Fixation in Red Clover (Trifolium pratense L.)

  • Oshadhi P. Athukorala Arachchige,
  • Pramod Rathor,
  • Hari P. Poudel and
  • Malinda S. Thilakarathna

Symbiotic nitrogen fixation (SNF) in forage legumes is a key biological process that provides nitrogen inputs and enhances soil fertility in agroecosystems. Enhancing SNF efficiency helps reduce dependence on synthetic nitrogen fertilizers and improv...

  • Review
  • Open Access
1 Citations
790 Views
19 Pages

Feeds rich in protein are the dominant nitrogen input in intensive aquaculture, yet only part of dietary nitrogen is retained as animal biomass; the remainder enters water and sediment through uneaten feed, feces, dissolved wastes, mucus, sloughed ti...

  • Article
  • Open Access
668 Views
21 Pages

This study aimed to investigate the effects of different nitrogen (N) fertilizer rates and sowing dates on grain yield, protein content, grain N uptake, and nitrogen recovery efficiency (NRE) in spring and facultative wheat (Triticum aestivum L.) cul...

  • Review
  • Open Access
1,758 Views
40 Pages

Remote Sensing and Machine Learning for Monitoring Soil Nitrogen Dynamics and Crop Nitrogen Status in Field Conditions

  • Boubacar Gano,
  • Dinesh Ghimire,
  • Serigne Mansour Diene,
  • Dhiraj Srivastava and ,
  • Daniel Kingsley Cudjoe and
  • Nadia Shakoor
  • + 1 author+ 0 author

Efficient nitrogen (N) management is essential for sustaining crop productivity while minimizing environmental impacts associated with nitrogen losses. However, the high spatial and temporal variability of soil nitrogen dynamics and crop nitrogen sta...

  • Article
  • Open Access
1 Citations
1,164 Views
28 Pages

Adaptation Mechanisms, Nutrient Dynamics and Nitrogen Use Efficiency of Rice Cultivars Under Tidal Floodplain Ecosystems

  • Md. Saddam Hossain,
  • Anika Tabassum,
  • Suhel Mia,
  • Muhammad Sajidur Rahman and
  • Md. Abdullah Al Mamun

Rice production in coastal tidal floodplains is severely constrained by periodic tidal submergence, making modern semi-dwarf varieties unsuitable. While local landrace cultivars are widely grown for their adaptability, their adaptation mechanisms and...

(This article belongs to the Special Issue Nitrogen Management in Plant Cultivation, 2nd Edition)
  • Article
  • Open Access
715 Views
23 Pages

Foliar Fertilization and Seed Priming Mitigate Acidic Soil Stress in Sunflower: Effects on Yield, Germination, and Biochemical Traits

  • Ivana Varga,
  • Đurđica Cerančević,
  • Dario Iljkić,
  • Miro Stošić and ,
  • Manda Antunović and
  • Dejan Agić
  • + 1 author+ 0 author

Acidic soil stress can limit sunflower productivity by reducing nutrient availability, impairing root development, and affecting early plant establishment, while foliar nutrient application and seed pretreatment may provide additional support under r...

(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
  • Article
  • Open Access
1,068 Views
15 Pages

Nitrogen is integral to all living systems, but its diverse forms are interconverted dynamically through reductions and oxidations that constitute the nitrogen cycle. Ecosystems gain combined nitrogen by bacterial and archaeal reduction in atmospheri...

(This article belongs to the Special Issue Nitrogen–Carbon Interactions in Global Biogeochemistry)
  • Article
  • Open Access
802 Views
14 Pages

Effect of Nitrogen Rate and Timing on Forage Sorghum Production and Quality

  • Ishneet Kaur,
  • Steve Phillips,
  • Steven Sawatzky and
  • Brian Arnall

Forage sorghum is widely used in the southern Great Plains because of its drought tolerance, high biomass productivity, and suitability for multi-cut hay systems; however, information on nitrogen (N) management for forage production and quality in th...

  • Article
  • Open Access
512 Views
17 Pages

Vertical Patterns and Responses of Soil Microbial Functional Groups to Chronic Nitrogen Addition and Their Linkages to Nutrient Dynamics in a Typical Steppe

  • Muqier Hasi,
  • Xinyi Jiang,
  • Shilin Wang,
  • Yasong Chen and ,
  • Canran Yang,
  • Jianhui Huang and
  • Guoxiang Niu
  • + 2 authors+ 0 author

Despite the pivotal importance of soil microbial communities in regulating the terrestrial carbon (C) cycle and ecosystem functioning, their vertical patterns and their responses to long-term nitrogen (N) deposition, in terms of microbial biomass and...

  • Article
  • Open Access
578 Views
13 Pages

Balanced fertilization is essential for improving the growth and productivity of Sapindus mukorossi plantations. In this study, a three-year “3414” fertilization experiment was conducted in a six-year-old plantation of the new S. mukoross...

  • Article
  • Open Access
962 Views
20 Pages

Effects of Foliar Application on Soybean Yield and Quality Traits

  • Adrian Negrea,
  • Raluca Rezi,
  • Alina Șimon,
  • Camelia Urdă and ,
  • Laura Șopterean and
  • Florin Russu
  • + 1 author+ 0 author

Adequate fertilization is essential for optimizing soybean productivity and seed quality, while supplementary fertilization plays a key role in correcting nutrient deficiencies and supporting plant performance under varying environmental conditions....

  • Review
  • Open Access
1 Citations
905 Views
23 Pages

Closing the Nitrogen Gap: Emissions, Efficiency, and Sensor-Based Monitoring in Agricultural Systems

  • Baber Ali,
  • Abdul Waheed,
  • Muhammad Siddique Afridi,
  • Aqsa Hafeez and
  • Nijat Imin

Global food demand is projected to rise by approximately 56 percent between 2010 and 2050, intensifying reliance on synthetic nitrogen fertilizer during a time when only about half of all applied nitrogen is recovered by crops, with the remainder spl...

(This article belongs to the Special Issue Nitrogen Cycling, Greenhouse Gas Emissions and Sustainable Nutrient Management in Soil Ecosystems)
  • Article
  • Open Access
573 Views
11 Pages

In animal production, gases such as ammonia and greenhouse gases (GHGs) originate from wastes in three main compartments: the animal house of an experimental facility in Northern Italy, storage sites and the slurry-spreading process on cultivated soi...

  • Review
  • Open Access
2 Citations
942 Views
20 Pages

Nitric oxide (NO) is a small, highly reactive gaseous signaling molecule that plays diverse and context-dependent roles in microbial physiology and host–microbe interactions. Over the past decade, increasing evidence has revealed the dual natur...

  • Article
  • Open Access
1 Citations
1,006 Views
22 Pages

A Novel Nitrogen-Fixing Subspecies of Rhizobium laguerreae Enhances Symbiotic Performance in Pisum sativum

  • Houda Ilahi,
  • Houda Zouagui,
  • Seif Allah Chihaoui,
  • Muhammad Sulman and ,
  • Nada Jihnaoui,
  • Mustapha Missbah El Idrissi,
  • Mohamed Najib Alfeddy,
  • Lahcen Ouahmane,
  • Hassen Gherbi and
  • Bacem Mnasri
  • + 7 authors+ 2 authors

This study investigates nitrogen-fixing rhizobia associated with Pisum sativum, a member of the tribe Vicieae (Fabaceae), whose species establish symbioses with bacteria belonging predominantly to the symbiovar viciae within the Rhizobium leguminosar...

(This article belongs to the Special Issue Genomics and Molecular Ecology of Microbial Nitrogen Fixation and Ecosystem Nitrogen Cycling)
  • Article
  • Open Access
823 Views
12 Pages

The Lag Effect of National Nitrogen Management on Nitrogen Use Efficiency and Greenhouse Gas Emission

  • Wangzheng Shen,
  • Qianrui Jing,
  • Sisi Li,
  • Yanhua Zhuang and ,
  • He Duan,
  • Junchong Wei,
  • Jing He,
  • Yun Du and
  • Liang Zhang
  • + 4 authors+ 0 author

Effective nitrogen (N) management is essential for ensuring food security, promoting agricultural sustainability, and addressing climate change due to the rise in fertilizer use since the 20th century. However, environmental improvements from N manag...

  • Review
  • Open Access
1,483 Views
23 Pages

Nitrogen Dynamics in Tropical Pastures: Relating Soil–Plant–Animal Interactions to Improve Productivity and Reduce Greenhouse Gas Emissions

  • Hitalo Rodrigues da Silva,
  • Gelson dos Santos Difante,
  • Francisca Fernanda da Silva Roberto,
  • Vanessa Zirondi Longhini and ,
  • Jéssica Gomes Rodrigues,
  • Marislayne de Gusmão Pereira,
  • Carolina Marques Costa Araújo,
  • Marcos Antonio Ferreira-Júnior,
  • Denise Baptaglin Montagner and
  • Vicente Batista de Souza-Junior
  • + 6 authors+ 1 author

Nitrogen fertilization plays a central role in the intensification and sustainability of tropical pasture systems by influencing forage production, animal performance, and greenhouse gas (GHG) emissions. Although the individual components of these sy...

  • Systematic Review
  • Open Access
2 Citations
1,175 Views
25 Pages

UAV-Based Nitrogen Assessment in Wheat: A Systematic Review of Target Traits, Validation Rigor, Growth Stage Evidence, and Machine Learning Approaches

  • Muhammad Waqar Nasir,
  • Muhammad Yousaf Nadeem,
  • Mawra Ishaq,
  • Rabia Manzoor and ,
  • Muhammad Haseeb Javaid,
  • Muhammad Daniyal Junaid and
  • Changwei Tan
  • + 2 authors+ 0 author

Excess or deficiency of nitrogen affects wheat yield significantly. Several destructive and non-destructive methods are used for nitrogen diagnosis to support precision fertilizer management in wheat. Recently, UAV-based remote sensing combined with...

  • Article
  • Open Access
1,012 Views
23 Pages

Halotolerant Nitrogen-Fixing Mesorhizobium ciceri Modulates Antioxidant Homeostasis and Growth Performance in Chickpea Cultivars Under Salt Stress

  • Imen Hemissi,
  • Hasna Ellouzi,
  • Amira Hachana,
  • Walid Zorrig and ,
  • Souhir Amraoui,
  • Hanen Arfaoui,
  • Mohsen Hnana and
  • Mohamed Annabi
  • + 3 authors+ 0 author

Soil salinity inhibits biological nitrogen fixation (BNF) in legumes, compromising nitrogen nutrition and crop productivity. This study evaluated whether two halotolerant Mesorhizobium ciceri strains (S1, S2) can sustain BNF and alleviate moderate sa...

(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
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Nitrogen - ISSN 2504-3129