Nitrogen: Advances in Plant Stress Research

A special issue of Nitrogen (ISSN 2504-3129).

Deadline for manuscript submissions: 31 December 2025 | Viewed by 2983

Special Issue Editor


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Guest Editor
Department of Genetics, Luiz de Queiroz College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba, São Paulo, SP, Brazil
Interests: antioxidants; plants; agriculture; plant physiology; biochemistry; stresses; heavy metals; genetics; molecular biology; omics; abiotic stresses; environmental stresses; cadmium; horticulture; crops

Special Issue Information

Dear Colleagues,

Environmental stresses pose major challenges to plant growth and productivity. Different abiotic stresses, such as drought, salinity, extreme temperatures, and nutrient stress, have a significant impact on plant physiological processes. Furthermore, contaminants such as heavy metals may compromise both environmental safety and plant metabolism. Biotic factors (e.g., pests, pathogens, and parasites) further stress plant development. Other stresses involve UV and light stresses, oxidative stress, and pesticide exposure, which also come under this research focus. Importantly, both natural and anthropogenic stressors add to the complexity of environmental challenges faced by plants. Thus, these stress factors affect the growth and adaptability of plants and add complexity to plant responses in the scenario of environmental challenge. It is therefore important to understand how plants cope with such stressors, from mechanisms and the modulation of tolerance to the mitigation of stress, in order to enhance resilient traits that will ensure agricultural sustainability and related strategies.

Nitrogen is a very important macronutrient in plants. The study of nitrogen dynamics, from uptake and transport to accumulation, provides important information on how plants cope with adverse conditions of the environment and minimize their stress effects.

This Special Issue, entitled "Nitrogen: Advances in Plant Stress Research," aims to cover new and recent insights into nitrogen metabolism interactions with plant responses to stress and the mechanisms involved. The various aspects of nitrogen dynamics in plants covered in this Special Issue range from uptake and transport to accumulation, including genetic, biochemical, molecular, and physiological mechanisms in the context of plant stress research. Research focused on exogenous nitrogen application is also relevant in order to improve plant resilience amidst growing stress challenges.

Our research focus also extends to the aspects of plant response to stresses at all levels of observation, from morphological and anatomical features through epigenetic modifications, offering a wide-ranging view of how plants adapt to stresses. We also welcome studies on stress factors separately and also the interactions between different stressors. We also invite research contributions dealing with interactions affecting the way nitrogen metabolism is modulated to ensure and/or enhance its tolerance against these stresses. We also encourage the submission of manuscripts dealing with crosstalk between two distinct types of stressor responses, as well as original research addressing the role of symbiotic associations, including that of mycorrhizal fungi and N-fixing bacteria, in enhancing N-use efficiency and stress tolerance. From crops to model plants, the Special Issue welcomes submissions on a wide range of plant species.

The Special Issue calls for original research articles, reviews, opinions, perspectives, and communications from vast groups of disciplines. Contributions related to physiological processes, molecular pathways, biochemical interactions in nitrogen metabolism, and studies concerned with the modulation given to these processes under stress conditions are encouraged. Scientific works using approaches based on emerging technologies are also encouraged. This issue tries to fill this gap, from the basic level of scientific research to practical applications that can give insights into field-level practices and strategies. It is here that an understanding of how nitrogen dynamics could mitigate the effects of stress will be key in the development of sustainable agricultural practices in light of continuing climate change and global food demands.

We look forward to your valuable contributions and further advancing this critical field with you.

Dr. Deyvid Novaes Marques
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Nitrogen is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • abiotic stress
  • biotic stress
  • environmental science
  • genetics
  • nitrogen
  • plants
  • physiology
  • biochemistry

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Published Papers (3 papers)

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Research

18 pages, 6221 KiB  
Article
Dynamics of SPAD Index, Leaf Pigment, and Macronutrient Relationships in Sugar Beet Leaves Under Spring Nitrogen Fertilization
by Ivana Varga, Milan Pospišil, Dario Iljkić, Antonela Markulj Kulundžić, Monika Tkalec Kojić and Manda Antunović
Nitrogen 2025, 6(1), 10; https://doi.org/10.3390/nitrogen6010010 - 18 Feb 2025
Viewed by 495
Abstract
Nitrogen fertilization greatly affects the development of sugar beet leaf and photosynthetic activity. This study aimed to evaluate the dynamics of leaf SPAD index, chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoids (Caro), and the macronutrient status [...] Read more.
Nitrogen fertilization greatly affects the development of sugar beet leaf and photosynthetic activity. This study aimed to evaluate the dynamics of leaf SPAD index, chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoids (Caro), and the macronutrient status (N, P, K, Na, Mg) in different N fertilization rates in sugar beet production. This study set up a two-year field experiment in Eastern Croatia. The N fertilization rate was applied as: N0—control, N1—only presowing fertilization (45 kg/ha), and N2—presowing with topdressing (99 kg/ha in 2014 and 85.5 kg/ha in 2015). In general, N fertilization has a significant (p ≤ 0.05) influence on leaf pigments, except for Chl b. With the highest N dose (N2), the Chl content in the leaves increased by 12% compared to the control treatment (0.75 mg/g FW). The Caro dynamics in the leaves of vegetative growth were significantly different (p ≤ 0.05); leaves in the younger growth stage at the end of May had the highest Caro content (0.011 mg/g FW). In general, the SPAD index was significantly different (p ≤ 0.05), among N fertilization, whereas the lowest SPAD was found at the control treatment (38.7) and the highest at the N2 treatment (40.8). In general, regarding nitrogen fertilization, the lowest SPAD readings had sugar beet leaves at the control treatment (38.7), whereas the highest was determined at the N2 treatment (40.8). A strong positive relationship (p ≤ 0.01) was determined for Chl a, Chl b, Chl a + b, and Chl a + b/Caro with the SPAD index, whereas an inverse relationship with the SPAD index was determined for Caro and Chl a/b. The results demonstrate that nitrogen application, particularly at higher rates, positively influences chlorophyll and carotenoid content, as well as overall plant health, which can inform agricultural practices for more sustainable and efficient sugar beet cultivation. Full article
(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
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19 pages, 310 KiB  
Article
Genetic Parameters and Trait Associations in Wheat Under Drought and Low Nitrogen Conditions
by Sbongeleni Warren Duma, Hussein Shimelis, Seltene Abady Tesfamariam and Toi John Tsilo
Nitrogen 2024, 5(4), 1196-1214; https://doi.org/10.3390/nitrogen5040076 - 23 Dec 2024
Viewed by 840
Abstract
Drought and low nitrogen stress are the leading cause of low crop production and productivity worldwide. Developing drought-tolerant wheat germplasm resilient to low nitrogen conditions is essential through genetic enhancement and selection for novel traits. The objective of the research was to investigate [...] Read more.
Drought and low nitrogen stress are the leading cause of low crop production and productivity worldwide. Developing drought-tolerant wheat germplasm resilient to low nitrogen conditions is essential through genetic enhancement and selection for novel traits. The objective of the research was to investigate genetic diversity, parameters, and trait relationships within a wheat genotype panel to inform drought- and low-N-tolerant variety selection. This study evaluated 50 wheat genotypes under drought-stressed and non-stressed conditions, with varying nitrogen (50 kg ha−1, 100 kg ha−1, and 200 kg ha−1) levels across four testing sites during the 2019/2020 growing season. Statistical analysis (combined ANOVA) revealed substantial genetic variation (p < 0.05) for the majority of tested traits. High heritability and substantial genetic gain for KPS (97.49%, 28.10% GAM) and SE (96.48%, 14.28% GAM) were determined under drought and low nitrogen stress. Under drought-stressed and non-stressed (at 200 kg N ha−1), grain yield expressed high heritability estimates of 80.43% and 75.68% and genetic advance at 21.90% and 21.56%, respectively. Positive and significant correlations (r > 0.5; p < 0.001) were measured between grain yield and yield components, implicating simultaneous direct and indirect selection of desired traits. The positive relationship between grain yield and yield components suggests that further quantitative trait loci analysis and progeny testing are crucial to guide genotype selection and breeding for drought and low-N stress-tolerant wheat genotypes. Full article
(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
25 pages, 3273 KiB  
Article
Synergistic Effect of Iron and Zinc Nanoparticles with Recommended Nitrogen Dose on Production and Grain Quality of Maize (Zea mays L.) Cultivars Under Drought Stress
by Mohamed Abbas, Chunjie Tian, Mohamed K. I. Nagy, Maryam Sabry Al-Metwally, Xuewen Chen and Hashim M. Abdel-Lattif
Nitrogen 2024, 5(4), 1156-1180; https://doi.org/10.3390/nitrogen5040074 - 18 Dec 2024
Viewed by 1092
Abstract
Abiotic factors, such as drought, can significantly impact the vegetative growth and productivity of maize. To investigate the effects of the combined foliar application of zinc (Zn) and iron (Fe) nanoparticles with the recommended nitrogen dose (RND) on maize production and grain chemical [...] Read more.
Abiotic factors, such as drought, can significantly impact the vegetative growth and productivity of maize. To investigate the effects of the combined foliar application of zinc (Zn) and iron (Fe) nanoparticles with the recommended nitrogen dose (RND) on maize production and grain chemical composition under different water regimes, two field experiments were conducted in El-Ayyat city, Giza, Egypt, during the summer seasons of 2022 and 2023. This study utilized a split-split-plot experimental design with three replications. The main plots were designated to different water regimes (100, 80, 60, and 40% of estimated evapotranspiration), while the sub-plots were randomly distributed with Zn and Fe nanoparticle concentrations (0, 100, and 200 mg/L). The sub-sub-plots were randomly allocated to three maize cultivars (SC-P3062, SC-32D99, and SC-P3433). The results revealed that exposure to drought conditions resulted in a significant decline in the yield and yield-related attributes across all maize cultivars examined. Grain yield decreased by 10–50% under drought conditions. However, the foliar application of Zn and Fe nanoparticles was found to significantly improve grain yield, protein content, oil content, starch content, crude fiber, ash, and macro- and micronutrient concentrations in the maize cultivars under control and drought stress conditions. The foliar application of Zn and Fe nanoparticles at a concentration of 200 mg/L to the SC-P3433 maize cultivar led to the greatest grain yield per hectare, reaching 11,749 and 11,657 kg under the irrigation regimes with 100 and 80% total evapotranspiration, respectively. According to the assessment using the relative drought index, the SC-P3062 maize cultivar demonstrated tolerance (T) to water stress conditions. In conclusion, the foliar application of Zn and Fe nanoparticles (100–200 mg/L) effectively mitigated the negative effects of drought stress on maize plants. This approach can be recommended for farmers in arid and semi-arid regions to maintain and improve maize yield and grain quality under water-deficit conditions. Full article
(This article belongs to the Special Issue Nitrogen: Advances in Plant Stress Research)
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