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Fermentation, Volume 11, Issue 8

2025 August - 68 articles

Cover Story: Weizmannia ginsengihumi was isolated from sugarcane bagasse for the production of lactic acid by xylose fermentation. Response surface analysis indicated that the optimal concentrations of carbon and nitrogen sources were 32.5 and 3.0 g·L−1, respectively, without the need to add sodium acetate to the culture medium, leading to the production of 20.02 ± 0.19 g·L−1, a productivity of 0.55 g/L/h after 36 h of fermentation, and a residual sugar concentration of 12.59 ± 0.51 g·L−1. The results obtained demonstrate the potential of Weizmannia ginsengihumi in producing lactic acid from biomass, thereby contributing to sustainability. View this paper
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Articles (68)

  • Article
  • Open Access
1,199 Views
13 Pages

This study investigated the effects of applied voltage on methane fermentation using separate reactors for the anode and cathode, with activated carbon felt as electrodes and a constant voltage of 0.7 V. Compared to the control, the cathode reactor e...

  • Article
  • Open Access
2 Citations
1,804 Views
16 Pages

Carotenoid Production by Dunaliella salina with Magnetic Field Application

  • Izabela Queiroz Silva,
  • Bruno Roswag Machado,
  • Tamires Machado Ferreira,
  • Júlia de Farias Borges,
  • Cláudia Maria Luz Lapa Teixeira and
  • Lucielen Oliveira Santos

The use of external triggers in microalgae cultivation has emerged as a promising approach to enhance biomass production and biochemical composition. For instance, magnetic fields (MFs) have had their potential to modulate cellular metabolism and phy...

  • Article
  • Open Access
913 Views
13 Pages

In this study, we developed a combination strategy of bioenzymes and sophorolipid (SL) co-pretreatment to enhance volatile fatty acids (VFAs) in co-fermentation of waste activated sludge (WAS) and rubberwood hydrolysates (RWHs). Among all the pretrea...

  • Article
  • Open Access
1,381 Views
17 Pages

Volatilomic and Sensorial Profiles of Cabernet Sauvignon Wines Fermented with Different Commercial Yeasts

  • Alejandra Chávez-Márquez,
  • Alfonso A. Gardea,
  • Humberto González-Rios,
  • Maria del Refugio Robles-Burgueño and
  • Luz Vázquez-Moreno

Volatilomic and sensory analyses of wine are excellent tools for enologists and winemakers when selecting commercial yeast based on the production of metabolites related to desirable wine characteristics. Integrating this holistic approach could lead...

  • Article
  • Open Access
2 Citations
1,000 Views
14 Pages

Studying the uses of different organic-mineral rocks is an expanding area of research. Although these materials have primarily been used in forestry and agriculture, other potential applications include cosmetics and nutrition. Alginite is a volcanic...

  • Article
  • Open Access
1 Citations
1,295 Views
19 Pages

A Comparative Proteomic Analysis of the Acetification Process of Komagataeibacter europaeus Using Different Substrates

  • Daniela Herrera-Rosero,
  • Juan J. Román-Camacho,
  • Juan Carlos García-García,
  • Inés M. Santos-Dueñas,
  • Teresa García-Martínez,
  • Isidoro García-García and
  • Juan Carlos Mauricio

Although vinegar is technically elaborated by a well-known bioprocess, the behavior and function of the microorganisms responsible for its production still need investigation. In vinegars obtained from raw materials and systems typical of Europe, the...

  • Article
  • Open Access
1,850 Views
18 Pages

Cutinase Production in Komagataella phaffii (Pichia pastoris): Performance Differences Between Host Strains

  • Andrea Sabido-Ramos,
  • Montserrat Tagle-Gil,
  • Krystel Estefany León-Montes,
  • José Augusto Castro-Rodríguez and
  • Amelia Farrés

The Pichia system has been exploited for decades as a host for recombinant protein production, but there is still an information gap regarding problems that may arise with its use. The application of strains based on the methanol-induced alcohol oxid...

  • Review
  • Open Access
3 Citations
4,686 Views
26 Pages

The growing demand for health-promoting food products has led to increased efforts to develop formulations enriched with probiotics and dietary fiber (DF). While traditional fermented foods remain widely recognized sources of probiotics, there is a p...

  • Article
  • Open Access
3 Citations
1,334 Views
21 Pages

Tropical forage crops vary widely in biochemical composition, resulting in inconsistent silage quality. Understanding how plant traits shape microbial and metabolic networks during ensiling is crucial for optimizing fermentation outcomes. Eight tropi...

  • Article
  • Open Access
1,261 Views
16 Pages

Zaolajiao (ZLJ) is a traditional national specialty fermented condiment in Guizhou, and organic acid is one of its main flavor substances. In the study, we used metabolomics and multivariate analysis to identify differential organic acids (DOAs) duri...

  • Article
  • Open Access
1,408 Views
15 Pages

Collagenase Production from Aspergillus serratalhadensis URM 7866 Using Industrial By-Products: Purification and Characterization

  • Luiz Henrique Svintiskas Lino,
  • Kethylen Barbara Barbosa Cardoso,
  • Pietra Gícia Oliveira Cosmo da Silva,
  • Raphael Luiz Andrade Silva,
  • Maria Eduarda Luiz Coelho de Miranda,
  • Daniel Charles dos Santos Macêdo,
  • Ana Lúcia Figueiredo Porto,
  • Cristina Maria de Souza Motta,
  • Marcia Nieves Carneiro da Cunha and
  • Daniela de Araújo Viana Marques
  • + 3 authors

Collagenases are enzymes with broad biotechnological applications in medicine. This study describes the production and characterization of a collagenase from Aspergillus serratalhadensis URM 7866, isolated from the Caatinga biome. Solid-state ferment...

  • Review
  • Open Access
2,376 Views
28 Pages

Recent Advances in Microbial Bioconversion as an Approach to Boost Hydroxytyrosol Recovery from Olive Mill Wastewater

  • Irene Maria Zingale,
  • Anna Elisabetta Maccarronello,
  • Claudia Carbone,
  • Cinzia Lucia Randazzo,
  • Teresa Musumeci and
  • Cinzia Caggia

Olive mill wastewater (OMWW) is a highly complex matrix derived from olive oil extraction, containing phenolic compounds, lipids, minerals, and organic acids. Hydroxytyrosol (HT), an outstanding antioxidant and health-promoting phenolic compound, has...

  • Article
  • Open Access
3,153 Views
17 Pages

Banana Pseudostem By-Product: A Sustainable Source of Prebiotics and Protection for Probiotic Lactic Acid Bacteria Under Gastrointestinal Conditions

  • Márcia Maria de Souza Moretti,
  • Tais Fernanda Borgonovi,
  • Svetoslav Dimitrov Todorov and
  • Ana Lúcia Barretto Penna

Agricultural by-products, such as banana pseudostems (BPS), present a sustainable solution for waste reduction and the recovery of valuable metabolites with biotechnological applications. This study investigated the potential of BPS as a substrate fo...

  • Article
  • Open Access
4 Citations
1,471 Views
15 Pages

In recent years, ultrasound has been integrated into fermentation technology due to its activating effect on microorganisms, and the possible effects of ultrasound-assisted fermentation on the fermentation process, yield and quality of the final prod...

  • Article
  • Open Access
1,186 Views
20 Pages

Probiotic Potential of Some Lactic Acid Bacteria Isolated from Blue Maize Atole Agrio from Veracruz, México

  • Margarita Torres-Gregorio,
  • Rosa María Ribas-Aparicio,
  • María Guadalupe Aguilera-Arreola,
  • Gustavo F. Gutiérrez-López and
  • Humberto Hernández-Sánchez

Mexican culture offers a great variety of traditional maize-based fermented foods that are beneficial for human health. Atole agrio (sour atole), prepared from blue maize (Zea mays) in the state of Veracruz, has been scarcely studied as a potential f...

  • Article
  • Open Access
1,497 Views
19 Pages

The Effect of Ruminal Fluid Adaptation to a Direct Fed Microbial: In Vitro Methane Production and Fermentation Characteristics

  • Sreemol Suthan Nair,
  • S. Richard O. Williams,
  • Aodán S. ó Neachtain,
  • Renata Tognelli,
  • Subhash Chandra,
  • Pablo S. Alvarez-Hess,
  • Long Cheng,
  • Khageswor Giri and
  • Joe L. Jacobs

Direct-fed microbials (DFM) have emerged as a promising dietary strategy for enteric methane abatement. However, it is unclear whether in vitro studies trialing DFM should use ruminal fluid previously adapted to the DFM of interest or if the DFM can...

  • Article
  • Open Access
2 Citations
1,102 Views
15 Pages

Composting is an effective method for stabilizing and valorizing pig manure, which is rich in nutrients but also contains heavy metals such as chromium (Cr). These heavy metals can promote the development of heavy metal resistance genes (MRGs) during...

  • Article
  • Open Access
1,485 Views
16 Pages

Production and Characterization of a Novel Glycolipid Biosurfactant from Bradyrhizobium sp.

  • Marcos André Moura Dias,
  • Eduardo Luiz Rossini,
  • Douglas de Britto and
  • Marcia Nitschke

Biosurfactants (BS) are surface-active compounds synthesized by microorganisms with broad industrial applications. Although BS-producing strains are widely reported, little is known about their production by diazotrophic bacteria. This study investig...

  • Article
  • Open Access
1 Citations
2,585 Views
14 Pages

Bacteriocins are ribosomal synthesized antimicrobial peptides produced by bacteria, but their low yields limit industrial applications as food preservatives. This study aimed to optimize the culture conditions of Pediococcus acidilactici CCFM18 and i...

  • Article
  • Open Access
1 Citations
976 Views
21 Pages

Digestate-Based Liquid Growth Medium for Production of Microbial Chitosan

  • Silvia Crognale,
  • Cristina Russo,
  • Eleonora Carota,
  • Ilaria Armentano,
  • Federico Di Gregorio,
  • Alessandro D’Annibale,
  • Alessio Cimini and
  • Maurizio Petruccioli

This study investigated the feasibility of using both the solid and the liquid fractions of waste from the anaerobic digestion process—the digestate—as a possible liquid growth medium for fungal production of chitosan. An enriched liquid...

  • Article
  • Open Access
1 Citations
916 Views
14 Pages

Modulating Fermentation in Total Mixed Ration Silages Using Lasalocid Sodium and Essential Oils

  • Isabele Paola de Oliveira Amaral,
  • Mariany Felex de Oliveira,
  • Marco Antonio Previdelli Orrico Junior,
  • Marciana Retore,
  • Tatiane Fernandes,
  • Yara América da Silva,
  • Ana Carolina Amorim Orrico,
  • Ronnie Coêlho de Andrade and
  • Giuliano Reis Pereira Muglia

This study evaluated the effects of lasalocid sodium (LASA) and essential oils on the fermentation and nutritional quality of total mixed ration (TMR) silages. A 4 × 2 factorial design tested four additives—a control (distilled water), LA...

  • Article
  • Open Access
1,812 Views
15 Pages

Plant-based yogurts are increasingly recognized as sustainable and health-conscious alternatives to dairy-based products, driven by environmental, ethical, and nutritional motivations. Pistachio milk, derived from an efficient and resilient crop, eme...

  • Article
  • Open Access
2,307 Views
15 Pages

Nowadays, there is interest in yogurts and fermented milks with incorporated fruits to fulfill the growing demand for healthier, nutritional, and functional foods. In the present study, the potential of apple pulp incorporation into yogurts was evalu...

  • Article
  • Open Access
1 Citations
3,438 Views
13 Pages

Isolation and Characterization of Low-Temperature and High-Salinity Amylase from Halomonas sp. KS41843

  • Jin A Kim,
  • Min Ju Kim,
  • Joung Han Yim,
  • Il-Chan Kim,
  • Jae-Sung Rhee and
  • Se Jong Han

The polar regions harbor uniquely diverse organisms adapted to low temperatures. Strains obtained from these regions are likely to produce enzymes that are industrially useful at low temperatures. In this study, a Halomonas sp. strain isolated from t...

  • Article
  • Open Access
1,267 Views
17 Pages

Synthesis of 3,4-Dihydroxybenzoic Acid in E. coli and C. glutamicum Using Dehydroshikimate Dehydratase of Different Types

  • Ekaterina Shmonova,
  • Arina Kruglova,
  • Nikita Nikandrov,
  • Nataliya Stoynova and
  • Vera Doroshenko

Dehydroshikimate (DHS) dehydratase (DSD) catalyzes the conversion of DHS into 3,4-dihydroxybenzoic acid (3,4-DHBA), a compound with promising applications across various industries. The DSD from Podospora anserina (DSDPa) was characterized and its ca...

  • Review
  • Open Access
1 Citations
2,675 Views
24 Pages

Exploring Exopolysaccharides Produced in Indigenous Mexican Fermented Beverages and Their Biotechnological Applications

  • Julián Fernando Oviedo-León,
  • Abril Ramírez Higuera,
  • Jorge Yáñez-Fernández,
  • Humberto Hernández-Sánchez and
  • Diana C. Castro-Rodríguez

Indigenous Mexican fermented beverages, such as pulque, colonche, tepache, and water kefir, are pillars of the country’s cultural and gastronomic heritage. Their sensory attributes and health-promoting properties arise from complex microbial co...

  • Article
  • Open Access
3 Citations
1,967 Views
18 Pages

Spirulina’s (Arthrospira platensis) use in food applications is limited by its dark color and sulfurous odor. This study aimed to develop a preharvest bioprocessing strategy using Saccharomyces cerevisiae co-cultivation to address these limitat...

  • Article
  • Open Access
1,251 Views
13 Pages

The Role of Quorum Sensing in Enhancing Lovastatin and Pigment Production in Monascus purpureus C322

  • Sirisha Yerramalli,
  • Stephen J. Getting,
  • Godfrey Kyazze and
  • Tajalli Keshavarz

Monascus purpureus is a filamentous fungus known for producing pharmaceutically valuable secondary metabolites, including azaphilone pigments and lovastatin. Lovastatin is an HMG-CoA reductase inhibitor widely used to manage hypercholesterolaemia, wh...

  • Article
  • Open Access
1 Citations
1,957 Views
14 Pages

This study aimed to identify a probiotic bacterium with antagonistic activity against the foodborne pathogen Staphylococcus aureus (S. aureus) and investigate the mechanism of its antibacterial components. Growth kinetics were analyzed to assess bact...

  • Article
  • Open Access
1 Citations
1,286 Views
18 Pages

Neuroprotective Properties of Fermented Malted Rice Obtained Under Different Processing Conditions

  • Micaela Albarracín,
  • Franco Van de Velde,
  • Raúl E. Cian and
  • Silvina R. Drago

Two products fermented with lactic acid bacteria (LAB) were obtained using malted rice (FR) and mashed malted rice (FMR). Peptide, phenolic acids, and γ-aminobutyric acid (GABA) contents and neuroprotective activities were evaluated before and...

  • Review
  • Open Access
2 Citations
3,456 Views
26 Pages

In Silico Modeling of Metabolic Pathways in Probiotic Microorganisms for Functional Food Biotechnology

  • Baiken B. Baimakhanova,
  • Amankeldi K. Sadanov,
  • Irina A. Ratnikova,
  • Gul B. Baimakhanova,
  • Saltanat E. Orasymbet,
  • Aigul A. Amitova,
  • Gulzat S. Aitkaliyeva and
  • Ardak B. Kakimova

Recent advances in computational biology have provided powerful tools for analyzing, modeling, and optimizing probiotic microorganisms, thereby supporting their development as promising agents for improving human health. The essential role of the mic...

  • Review
  • Open Access
10 Citations
6,056 Views
20 Pages

Anaerobic digestion (AD) is a sustainable and widely adopted technology for the treatment of organic solid wastes (OSWs). However, AD efficiency varies significantly across different substrates, primarily due to differences in the microbial community...

  • Article
  • Open Access
3 Citations
1,790 Views
22 Pages

Bioconversion of Olive Pomace: A Solid-State Fermentation Strategy with Aspergillus sp. for Detoxification and Enzyme Production

  • Laura A. Rodríguez,
  • María Carla Groff,
  • Sofía Alejandra Garay,
  • María Eugenia Díaz,
  • María Fabiana Sardella and
  • Gustavo Scaglia

This study aimed to evaluate solid-state fermentation (SSF) as a sustainable approach for the simultaneous detoxification of olive pomace (OP) and the production of industrially relevant enzymes. OP, a semisolid byproduct of olive oil extraction, is...

  • Article
  • Open Access
1 Citations
3,608 Views
20 Pages

Production of Vegan Ice Cream: Enrichment with Fermented Hazelnut Cake

  • Levent Yurdaer Aydemir,
  • Hande Demir,
  • Zafer Erbay,
  • Elif Kılıçarslan,
  • Pelin Salum and
  • Melike Beyza Ozdemir

The growing demand for sustainable plant-based dairy alternatives has spurred interest in valorizing agro-industrial byproducts like hazelnut cake, a protein-rich byproduct of oil extraction. This study developed formulations for vegan ice cream usin...

  • Article
  • Open Access
1 Citations
2,859 Views
19 Pages

The Production Optimization of a Thermostable Phytase from Bacillus subtilis SP11 Utilizing Mustard Meal as a Substrate

  • Md. Al Muid Khan,
  • Sabina Akhter,
  • Tanjil Arif,
  • Md. Mahmuduzzaman Mian,
  • Md. Arafat Al Mamun,
  • Muhammad Manjurul Karim and
  • Shakila Nargis Khan

Phytate, an antinutritional molecule in poultry feed, can be degraded by applying phytase, but its use in low- and middle-income countries is often limited due to importation instead of local production. Here, inexpensive raw materials were used to o...

  • Article
  • Open Access
1 Citations
1,198 Views
14 Pages

Circulating of In Situ Recovered Stream from Fermentation Broth as the Liquor for Lignocellulosic Biobutanol Production

  • Changsheng Su,
  • Yunxing Gao,
  • Gege Zhang,
  • Xinyue Zhang,
  • Yating Li,
  • Hongjia Zhang,
  • Hao Wen,
  • Wenqiang Ren,
  • Changwei Zhang and
  • Di Cai

Developing a more efficient, cleaner, and energy-saving pretreatment process is the primary goal for lignocellulosic biofuels production. This study demonstrated the feasibility of circulating high-concentration acetone–butanol–ethanol (A...

  • Article
  • Open Access
3 Citations
3,388 Views
21 Pages

Production of a Biosurfactant for Application in the Cosmetics Industry

  • Ana Paula Barbosa Cavalcanti,
  • Gleice Paula de Araújo,
  • Káren Gercyane de Oliveira Bezerra,
  • Fabíola Carolina Gomes de Almeida,
  • Maria da Glória Conceição da Silva,
  • Alessandra Sarubbo,
  • Cláudio José Galdino da Silva Júnior,
  • Rita de Cássia Freire Soares da Silva and
  • Leonie Asfora Sarubbo

The cosmetics industry has been seeking to develop products with renewable natural ingredients to reduce the use of or even replace synthetic substances. Biosurfactants can help meet this demand. These natural compounds are renewable, biodegradable,...

  • Article
  • Open Access
1,350 Views
22 Pages

Impact of Heat Stress on Rumen Fermentation Patterns and Microbiota Diversity and Its Association with Thermotolerance in Indigenous Goats

  • Mullakkalparambil Velayudhan Silpa,
  • Veerasamy Sejian,
  • Chinnasamy Devaraj,
  • Artabandhu Sahoo and
  • Raghavendra Bhatta

Goats are considered to be the ideal climate-resilient animal species in the tropics. Fewer studies are documented assessing the heat stress response of caprine ruminal microbiota, which can also be a crucial indicator of the resilience and/or adapta...

  • Article
  • Open Access
4 Citations
3,952 Views
17 Pages

Impact of Selected Starter-Based Sourdough Types on Fermentation Performance and Bio-Preservation of Bread

  • Khadija Atfaoui,
  • Sara Lebrazi,
  • Anas Raffak,
  • Youssef Chafai,
  • Karima El Kabous,
  • Mouhcine Fadil and
  • Mohammed Ouhssine

The aim of this study is to evaluate the effects of different types of sourdough (I to IV), developed with a specific starter culture (including Lactiplantibacillus plantarum, Levilactobacillus brevis, and Candida famata), on bread fermentation perfo...

  • Article
  • Open Access
1 Citations
1,465 Views
13 Pages

Biorefinery-Based Energy Recovery from Algae: Comparative Evaluation of Liquid and Gaseous Biofuels

  • Panagiotis Fotios Chatzimaliakas,
  • Dimitrios Malamis,
  • Sofia Mai and
  • Elli Maria Barampouti

In recent years, biofuels and bioenergy derived from algae have gained increasing attention, fueled by the growing demand for renewable energy sources and the urgent need to lower CO2 emissions. This research examines the generation of bioethanol and...

  • Article
  • Open Access
1,656 Views
14 Pages

To explore an industrial fermentation approach for traditional mung bean sour pulp, this study isolated core microorganisms including lactic acid bacteria and yeasts from naturally fermented samples and constructed a synthetic microbial community. Th...

  • Article
  • Open Access
1,419 Views
15 Pages

Biotechnological Potential of Weizmannia ginsengihumi in the Conversion of Xylose into Lactic Acid: A Sustainable Strategy

  • Larissa Provasi Santos,
  • Ingrid Yoshimura,
  • Fernanda Batista de Andrade and
  • Jonas Contiero

The aim of this study was to isolate Weizmannia spp. that produce lactic acid from xylose and use an experimental design to optimize the production of the metabolite. After isolation, the experiments were conducted in xylose-yeast extract-peptone med...

  • Article
  • Open Access
1,812 Views
20 Pages

During red wine production, managing the pomace cap is key for a successful fermentation, allowing the extraction of phenolics and other metabolites and providing the necessary oxygen for yeast activity. In recent years, automatic cap management syst...

  • Article
  • Open Access
3 Citations
1,420 Views
15 Pages

Degradation of Microplastics in an In Vitro Ruminal Environment

  • Sonia Tassone,
  • Rabeb Issaoui,
  • Valentina Balestra,
  • Salvatore Barbera,
  • Marta Fadda,
  • Hatsumi Kaihara,
  • Sara Glorio Patrucco,
  • Stefania Pragliola,
  • Vincenzo Venditto and
  • Khalil Abid

Microplastic (MP) pollution is an emerging concern in ruminant production, as animals are exposed to MPs through air, water, and feeds. Ruminants play a key role in MP transmission to humans via animal products and contribute to MP return to agricult...

  • Article
  • Open Access
3 Citations
2,050 Views
16 Pages

In this study, 62 lactic acid bacteria (LAB) strains were isolated from raw camel milk and evaluated for their probiotic potential. The strains exhibited significant variability in their ability to withstand simulated gastrointestinal conditions. Of...

  • Article
  • Open Access
1,388 Views
19 Pages

Monitoring C. vulgaris Cultivations Grown on Winery Wastewater Using Flow Cytometry

  • Teresa Lopes da Silva,
  • Thiago Abrantes Silva,
  • Bruna Thomazinho França,
  • Belina Ribeiro and
  • Alberto Reis

Winery wastewater (WWW), if released untreated, poses a serious environmental threat due to its high organic load. In this study, Chlorella vulgaris was cultivated in diluted WWW to assess its suitability as a culture medium. Two outdoor cultivation...

  • Article
  • Open Access
2 Citations
2,233 Views
18 Pages

Sustainable Production of Bacterial Cellulose in a Rotary Disk Bioreactor: Grape Pomace as a Replacement for the Carbon Source

  • Rodrigo Cáceres,
  • Patricio Oyarzún,
  • Juan Pablo Vargas,
  • Francisca Cuevas,
  • Kelly Torres,
  • Elizabeth Elgueta,
  • Irene Martínez and
  • Dariela Núñez

Bacterial nanocellulose (BNC) is a highly pure biopolymer with promising applications in the biomedical, food, and textile industries. However, the high production costs and low yields obtained in static conditions limit its scalability and industria...

  • Article
  • Open Access
1,756 Views
17 Pages

Physiological, Genetic, and Fermentative Traits of Oenococcus oeni Isolates from Spontaneous Malolactic Fermentation in Koshu Wine

  • Misa Otoguro,
  • Sayaka Inui,
  • Taichi Aoyanagi,
  • Ayana Misawa,
  • Hiromi Nakano,
  • Yoshimi Shimazu and
  • Shigekazu Misawa

Koshu wine, produced from the indigenous Japanese grape Vitis vinifera L. cv. Koshu exhibits a lower pH than other white wines, hindering malolactic fermentation (MLF) by lactic acid bacteria (LAB). Here, we aimed to isolate LAB strains capable of pe...

  • Article
  • Open Access
1,464 Views
23 Pages

The β-glucosidase CEL1B has been linked to regulating cellulase expression in Trichoderma reesei, yet its inducer-specific functions and broader regulatory roles remain poorly characterized. In this study, CRISPR-Cas9-mediated gene knockout was...

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Fermentation - ISSN 2311-5637