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Fermentation, Volume 11, Issue 10

October 2025 - 55 articles

Cover Story: α-Linolenic acid (ALA) is an essential omega-3 fatty acid with health benefits and applications in food and feed. Plant oils are the main source, but their limited supply challenges sustainability. We developed ALA production via microbial fermentation using Aspergillus oryzae, a Generally Recognized As Safe organism. By identifying its endogenous ω-3 desaturase gene (fad3), essential for ALA biosynthesis, and overexpressing fad3 through self-cloning, we obtained the Aofad3-MC strain, which achieved an ALA content of 30.7% of total lipids. Whole-genome and k-mer analyses confirmed the absence of vector-derived sequences. This strategy enables sustainable and socially acceptable ALA production and provides a platform for functional lipid biosynthesis in food, feed, and industrial applications. View this paper
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Articles (55)

  • Article
  • Open Access
614 Views
20 Pages

Optimization of Tiliacora triandra Leaf Extraction and Probiotic Fermentation for Developing a Functional Freeze-Dried Feed Supplements

  • Manatsanun Nopparatmaitree,
  • Tossaporn Incharoen,
  • Watcharapong Mitsuwan,
  • Atichat Thongnum,
  • Juan J. Loor and
  • Noraphat Hwanhlem

Tiliacora triandra (Yanang) leaf contains polyphenols, flavonoids, and mucilage polysaccharides with antioxidant and prebiotic functions, making it a promising substrate for probiotic fermentation. This study aimed to optimize Yanang extraction and s...

  • Article
  • Open Access
634 Views
14 Pages

Novel Nonlinear Control in a Chaotic Continuous Flow Enzymatic–Fermentative Bioreactor

  • Juan Luis Mata-Machuca,
  • Pablo Antonio López-Pérez and
  • Ricardo Aguilar-López

Fermentative processes are considered one of the most important technological developments in the modern transforming industry, due to this, the applied research to reach high performance standards with a crucial focus on system intensification, whic...

  • Article
  • Open Access
556 Views
17 Pages

β-Glucan from Highland Barley Spent Grain: Yield, Molecular Weight, Physicochemical Properties, Antioxidant Capacity, and Gel Characteristics

  • Suyang Li,
  • Lhundrup Namgyal,
  • Shiyi Chen,
  • Yong Zhou,
  • Afira Nayab,
  • Zhou Qin,
  • Dawa Dondup and
  • Ling Sun

β-Glucan from Tibetan highland barley (THB) is an excellent edible gel polysaccharide due to its unique hypoglycemic and antioxidant activities. However, direct extraction of β-glucan from THB exhibits low yields with higher costs. Given th...

  • Article
  • Open Access
663 Views
14 Pages

The financial feasibility of microbial carotenoid synthesis can be markedly improved by using widely available and renewable carbon sources. In this study, different carbon sources including molasses, were tested as carbon sources for Rhodococcus cor...

  • Article
  • Open Access
769 Views
21 Pages

Cottonseed meal (CSM), an important protein-rich feed ingredient, faces limited utilization in livestock diets due to the presence of free gossypol (FG)—a potent antinutritional toxin. This study aimed to isolate FG-degrading bacteria from the...

  • Article
  • Open Access
776 Views
15 Pages

The aim of the study was to investigate the effects of different concentrations (0.15, 0.30, and 0.45 mL/L) of essential oils from coriander seeds (Coriandrum sativum), tarragon (Artemisia dracunculus), and orange peels (Citrus sinensis) on biomass,...

  • Article
  • Open Access
970 Views
13 Pages

Isolation and Characterization of a High-Efficiency Algicidal Bacterium Streptomyces violaceorubidus lzh-14 Against the Harmful Cyanobacteria Microcystis aeruginosa

  • Zhe Zhao,
  • Dongying Zhao,
  • Yutong Wu,
  • Yibing Zhao,
  • Jie Qu,
  • Wentao Zheng,
  • Lei Fang,
  • Junhuan Gao,
  • Fei Liu and
  • Jihua Wang
  • + 1 author

Harmful cyanobacterial blooms (HABs), primarily composed of toxic cyanobacteria like Microcystis aeruginosa, pose a significant threat to aquatic ecosystems and human health. Algicidal bacteria had emerged as a promising strategy for HAB control due...

  • Article
  • Open Access
809 Views
22 Pages

Soybean, as a globally important economic crop, is severely threatened by Sclerotinia sclerotiorum, the causative agent of Sclerotinia stem rot (SSR), a major disease in soybean production worldwide, leading to significant yield losses and quality de...

  • Article
  • Open Access
807 Views
18 Pages

Evaluation of Nutritional Value and Rumen Degradation Rate of Six Unconventional Feeds Using In Vitro and In Situ Methods

  • Chen-Yang Shi,
  • Shi-Hong Yang,
  • Yin Ma,
  • Dong Chen,
  • Ze-Sheng Yan,
  • Guo-Hong Yuan,
  • Mu-Long Lu,
  • Qi-yu Diao,
  • Gui-Shan Xu and
  • Halidai Rehemujiang

Objective: This study systematically evaluated the nutritional compositions and bioactive compounds of six unconventional feed resources (Pepper residue (PR), Grape marc (MC), Pepper straw (PS), Lycium barbarum branches and leaves (LBBL), Licorice st...

  • Article
  • Open Access
717 Views
21 Pages

Meta-Analysis of Incorporating Camelina and Its By-Products into Ruminant Diets and Their Effects on Ruminal Fermentation, Methane Emissions, Milk Yield and Composition, and Metabolic Profile

  • Roshan Riaz,
  • Muhammad Waqas,
  • Ibrar Ahmed,
  • Hafiz Muhammad Nouman,
  • Beenish Imtiaz,
  • Mahmood Ul Hassan,
  • Massimo Todaro,
  • Riccardo Gannuscio,
  • Muhammad Naeem Tahir and
  • Ozge Sizmaz

The incorporation of Camelina sativa and its by-products (oil, meal, seeds, and expellers) into ruminant diets improves feed efficiency and reduces environmental impacts. This systematic review and meta-analysis, conducted in line with PRISMA guideli...

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Fermentation - ISSN 2311-5637