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Fermentation, Volume 11, Issue 1

2025 January - 44 articles

Cover Story: Developing a thermodynamic model for analyzing the relationship between externally applied energy and electrogenic microbial biomass is an essential step for the precise simulation result prediction of bioelectrochemical systems (BESs). In this study, we developed a bioenergetic model based on the thermodynamics of microbial yield to calculate the optimum voltage for organic matter oxidation at the bioanode, enabling efficient carbon dioxide (CO2) reduction to methane (CH4) through electromethanogenesis at the biocathode. The model identified applied voltage −0.3 V vs. SHE on the biocathode as optimal CH4 production and microbial growth, regardless of ohmic losses. Validation with experimental data from a lab-scale BES reactor has confirmed that simulation tools effectively support the up-scaling of experimental setups. View this paper
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Articles (44)

  • Article
  • Open Access
5 Citations
3,878 Views
15 Pages

A plant-based beverage enhanced with GABA was developed through serial co-fermentation using Leuconostoc citreum S5 and Lactiplantibacillus plantarum KS2020. The first lactic acid fermentation was performed by Leu. citreum S5 with a vegetable mixture...

  • Article
  • Open Access
4 Citations
1,703 Views
14 Pages

Production of an Extract with β-1,4-Xylanase Activity by Fusarium oxysporum f. sp. melonis on a Sonicated Brewer’s Spent Grain Substrate

  • Irma A. Arreola-Cruz,
  • Rosalba Troncoso-Rojas,
  • Francisco Vásquez-Lara,
  • Nina G. Heredia-Sandoval and
  • Alma R. Islas-Rubio

The Fusarium oxysporum species commonly found in soil include plant and human pathogens, and nonpathogenic species. F. oxysporum grown on lignocellulosic substrates under submerged conditions produces an extracellular enzyme profile with hemicellulol...

  • Article
  • Open Access
2 Citations
2,340 Views
21 Pages

Harnessing the Influence of Pressure and Nutrients on Biological CO2 Methanation Using Response Surface Methodology and Artificial Neural Network—Genetic Algorithm Approaches

  • Alexandros Chatzis,
  • Konstantinos N. Kontogiannopoulos,
  • Nikolaos Dimitrakakis,
  • Anastasios Zouboulis and
  • Panagiotis G. Kougias

The biological methanation process has emerged as a promising alternative to thermo-catalytic methods due to its ability to operate under milder conditions. However, challenges such as low hydrogen solubility and the need for precise trace element su...

  • Article
  • Open Access
2 Citations
2,238 Views
18 Pages

Batch Reactor Design and Conception at Laboratory Scale for Solid-State Anaerobic Digestion: Practical Comparison Between 3D-Printed Digesters and Conventional Methods

  • Arnaud Dujany,
  • Franco Otaola,
  • Laura André,
  • Amar Naji,
  • Denis Luart,
  • Mikel Leturia,
  • André Pauss and
  • Thierry Ribeiro

Solid-state anaerobic digestion (SS-AD) is a promising technology for treating organic waste and producing renewable energy. This study explores the feasibility of using 3D printing to rapidly design cost-effective laboratory-scale digesters for opti...

  • Article
  • Open Access
3 Citations
1,893 Views
18 Pages

Optimal product synthesis in bioelectrochemical systems (BESs) requires a comprehensive understanding of the relationship between external voltage and microbial yield. While most studies assume constant growth yields or rely on empirical estimates, t...

  • Article
  • Open Access
3 Citations
4,968 Views
18 Pages

Isolation and Characterization of Indigenous Acetobacter Strains from Cashew Apple and Their Potential Use in Vinegar Production

  • Yaya Anianhou Ouattara,
  • Doudjo Soro,
  • Kouakou Romain Fossou,
  • Ahou Cinthia Ines Yebouet,
  • Ismael Abraham Cissé,
  • Dogbo Marius Akissi,
  • Adolphe Zézé,
  • Emmanuel Assidjo and
  • Kouassi Benjamin Yao

Côte d’Ivoire has the largest cashew tree cultivation area in the world, but the cashew apple produced is still underutilized despite its potential for industrial use. The present study aimed to isolate and identify acetic acid bacterial...

  • Article
  • Open Access
1 Citations
3,293 Views
17 Pages

Economic development increases and brings about issues such as the secure supply of food in a sustainable way. Phytases are enzymes catalyzing phytate hydrolysis to release phosphorus in an inorganic form. Animal feeds could be supplemented with bact...

  • Article
  • Open Access
4 Citations
3,743 Views
27 Pages

A Novel Wild-Type Lacticaseibacillus paracasei Strain Suitable for the Production of Functional Yoghurt and Ayran Products

  • Ioanna Prapa,
  • Chrysoula Pavlatou,
  • Vasiliki Kompoura,
  • Anastasios Nikolaou,
  • Electra Stylianopoulou,
  • George Skavdis,
  • Maria E. Grigoriou and
  • Yiannis Kourkoutas

Raw goat and ewe’s milk samples were used for the isolation of seven lactic acid bacteria new strains. After testing hemolytic activity and resistance to antibiotics, specific functional properties were evaluated; Lactococcus lactis subsp. lact...

  • Review
  • Open Access
10 Citations
7,781 Views
25 Pages

Antimicrobial Peptides, Their Production, and Potential in the Fight Against Antibiotic-Resistant Pathogens

  • Margarita Saubenova,
  • Alexander Rapoport,
  • Zhanerke Yermekbay and
  • Yelena Oleinikova

The article reviews the literature on antimicrobial peptides (AMPs) that exhibit unique antimicrobial mechanisms, such as broad-spectrum activity, low development of antimicrobial resistance, and the ability to modulate the immune response of the hos...

  • Article
  • Open Access
2 Citations
1,657 Views
11 Pages

Manouri is a Greek whey cheese, with a Protected Denomination of Origin recognition, produced by heating the cheese whey and added milk and/or cream at high temperatures (88–90 °C) to form a coagulum. High-heat treatment results in the inac...

  • Article
  • Open Access
1,783 Views
12 Pages

Heterologous Expression and Functional Analysis of Exiguobacterium Algin Lyase Gene by Pichia pastoris

  • Hanwen Wu,
  • Kai Hou,
  • Yutong Jiang,
  • Mingjian Luan,
  • Yuxia Sun,
  • Xi He and
  • Xiangzhong Zhao

Algin is the most abundant substance in alga. Alginate lyase degrades algin and produces algin monosaccharides, disaccharides, and oligosaccharides, which are widely used in bioenergy, food, medicine, and other fields. In this study, one Exiguobacter...

  • Article
  • Open Access
1 Citations
1,947 Views
18 Pages

The Production of an Economical Culture Medium from Apple Pomace for the Propagation of Non-Conventional Cidermaking Yeast Starters

  • Josefina M. Fontanini,
  • Andrea C. Origone,
  • Marcela P. Sangorrín,
  • Carmen R. Maturano,
  • Christian A. Lopes and
  • M. Eugenia Rodríguez

The aim of the present study was to evaluate the use of different chemical treatments of apple pomace in order to produce an economical culture medium for the propagation of two non-conventional yeast strains. An experimental design approach was used...

  • Article
  • Open Access
2 Citations
3,191 Views
11 Pages

Enhancing Bioactive Cordycepin Production via Precision Fermentation with an Engineered Aspergillus oryzae

  • Jutamas Anantayanon,
  • Warinthon Chamkhuy,
  • Nakul Rattanaphan,
  • Sarocha Panchanawaporn,
  • Kobkul Laoteng and
  • Sukanya Jeennor

An optimal culture medium and highly stable biometabolites are important in industrial production processes. The response surface methodology with a Box–Behnken design was performed to determine the optimal culture medium of an engineered Asper...

  • Article
  • Open Access
2 Citations
2,440 Views
18 Pages

Carbon Dioxide Micro-Nano Bubbles Aeration Improves Carbon Fixation Efficiency for Succinic Acid Synthesis by Escherichia coli

  • Ying Chen,
  • Hao Wu,
  • Qianqian Huang,
  • Jingwen Liao,
  • Liuqing Wang,
  • Yue Pan,
  • Anming Xu,
  • Wenming Zhang and
  • Min Jiang

The low solubility of CO2 in water leads to massive CO2 emission and extremely low CO2 utilization in succinic acid (SA) biosynthesis. To enhance microbial CO2 utilization, micro-nano bubbles (MNBs) were induced in SA biosynthesis by E. coli Suc260 i...

  • Article
  • Open Access
6 Citations
5,317 Views
13 Pages

Co-Cultivations of Beauveria bassiana, Metarhizium anisopliae, and Trichoderma harzianum to Produce Bioactive Compounds for Application in Agriculture

  • Pauline Flores da Silva,
  • Maicon Sérgio Nascimento dos Santos,
  • Beatriz de Andrade Araújo,
  • Bruno Douglas Kerber,
  • Heloisa Alves Pinto de Oliveira,
  • Jerson Vanderlei Carús Guedes,
  • Marcio Antonio Mazutti,
  • Marcus Vinícius Tres and
  • Giovani Leone Zabot

Regenerative agriculture aims to improve soil quality and restore soil biodiversity, re-establishing natural systems in agricultural areas. Among some strategies, it is important to reduce the use of chemical pesticides that affect the productive cap...

  • Article
  • Open Access
1 Citations
1,468 Views
17 Pages

Optimizing Bacterial Protectant Composition to Enhance Baijiu Yeast Survival and Productivity During Spray Drying

  • Jingyu Li,
  • Fengkui Xiong,
  • Zhongbin Liu,
  • Jia Zheng,
  • Guangzhong Hu and
  • Zheng Feng

The flavor substances produced by the division of baijiu yeast during the winemaking process often determine the quality of white wine, and the difficulty of storing and transporting high-quality baijiu yeast is a bottleneck that restricts the develo...

  • Article
  • Open Access
1,236 Views
16 Pages

Ruminants rely on the rumen for the anaerobic fermentation of fibrous plant materials, facilitated by a complex microbial community of bacteria, archaea, fungi, and ciliates. Among them, ruminal ciliates significantly influence ruminal fermentation,...

  • Article
  • Open Access
2 Citations
1,913 Views
20 Pages

The Impact of Grape Maceration on Quality and Biogenic Amine Formation in Slovak Tokaj Wines: Examination of Microbial, Chemical and Sensory Properties

  • Boris Semjon,
  • Martin Bartkovský,
  • Peter Očenáš,
  • Ivana Regecová,
  • Zuzana Megyesy Eftimová,
  • Jana Výrostková,
  • Lýdia Mesarčová,
  • Mariana Kováčová,
  • Matúš Várady and
  • Slavomír Marcinčák
  • + 1 author

Grape maceration is essential in modulating the quality of Tokaj wines. The aim of this research was to analyse the effect of two maceration practices—one utilizing the application of a Saccharomyces cerevisiae culture and the other being macer...

  • Article
  • Open Access
3 Citations
2,052 Views
14 Pages

The Impact of Fermented Quinoa Sourdough with Enterococcus Strains on the Nutritional, Textural, and Sensorial Features of Gluten-Free Muffins

  • Souraya Sakoui,
  • Reda Derdak,
  • Fatimazahra Jouga,
  • Amal Dagni,
  • Oana Lelia Pop,
  • Dan Cristian Vodnar,
  • Bernadette-Emőke Teleky,
  • Maria Simona Chis,
  • Carmen Rodica Pop and
  • Bouchra El Khalfi
  • + 4 authors

Gluten-free baked goods exhibit reduced texture and taste characteristics compared to their gluten-containing counterparts. As a result, there is a renewed interest in the fermentation of gluten-free cereals with lactic acid bacteria, which is associ...

  • Article
  • Open Access
4 Citations
2,733 Views
13 Pages

High-Titer L-lactic Acid Production by Fed-Batch Simultaneous Saccharification and Fermentation of Steam-Exploded Corn Stover

  • Liheng Deng,
  • Changsheng Su,
  • Yilu Wu,
  • Qiang Xue,
  • Changwei Zhang,
  • Yong Wang,
  • Bin Wang and
  • Di Cai

Steam explosion (SE) is an effective lignocellulose pretreatment technology for second-generation L-lactic acid (L-LA) production. In this study, targeted to produce high-concentration L-LA from corn stover (CS), the fed-batch simultaneous saccharifi...

  • Review
  • Open Access
7 Citations
7,256 Views
25 Pages

Climate change and environmental impacts from greenhouse gas emissions have spurred on efforts to reduce these emissions. Meat production, especially from cattle, is a significant contributor, releasing methane—a greenhouse gas far more potent...

  • Review
  • Open Access
12 Citations
11,611 Views
22 Pages

Bacterial Cellulose: From Biofabrication to Applications in Sustainable Fashion and Vegan Leather

  • Dheanda Absharina,
  • Mohamad Padri,
  • Csilla Veres and
  • Csaba Vágvölgyi

The rising demand for sustainable materials has led to a significant focus on developing resources from renewable systems, particularly through the integration of biological processes. Bacterial cellulose (BC) has emerged as a highly promising biomat...

  • Article
  • Open Access
2,598 Views
24 Pages

Fermented vegetables contain probiotic microbes and metabolites, which are transformed from fresh vegetables, potentially providing health benefits. The kind of vegetable used to ferment and how it is grown may determine the types of health-promoting...

  • Article
  • Open Access
1 Citations
2,042 Views
20 Pages

During fermentation, bacterial and fungal species synthesize substrate-specific enzymes to obtain nutrients. During this process, potential allergenic products, including immunologically important gluten peptides, can be created. Current protocols fo...

  • Article
  • Open Access
9 Citations
7,330 Views
24 Pages

Pleurotus ostreatus: Nutritional Enhancement and Antioxidant Activity Improvement Through Cultivation on Spent Mushroom Substrate and Roots of Leafy Vegetables

  • Eirini-Maria Melanouri,
  • Ilias Diamantis,
  • Marianna Dedousi,
  • Eleni Dalaka,
  • Paraskevi Antonopoulou,
  • Seraphim Papanikolaou,
  • Ioannis Politis,
  • Georgios Theodorou and
  • Panagiota Diamantopoulou

Agricultural residues, including Pleurotus ostreatus spent mushroom substrate (SMS) and roots of (hydroponic) leafy vegetables (RLV), were tested in various proportions as substrates in new P. ostreatus cultivations, where wheat straw was the control...

  • Review
  • Open Access
13 Citations
4,757 Views
27 Pages

Membrane-Assisted Dark Fermentation for Integrated Biohydrogen Production and Purification: A Comprehensive Review

  • Octavio García-Depraect,
  • Laura Vargas-Estrada,
  • Raúl Muñoz and
  • Roberto Castro-Muñoz

The aim of this review is to provide a comprehensive analysis of the membrane-assisted dark fermentation process for bioH2 production and purification. This review initially analyses the need for and the current state of the art in H2 production thro...

  • Article
  • Open Access
2,081 Views
15 Pages

Impact of Dried Stems on the Chemical Profile of Passito Wines: A Case Study of Four Veneto Varieties

  • Giovanni Luzzini,
  • Loris Colognato,
  • Leonardo Vanzo,
  • Jessica Anahi Samaniego Solis,
  • Naíssa Prévide Bernardo,
  • Rosario Pascale,
  • Beatrice Perina,
  • Giacomo Cristanelli,
  • Maurizio Ugliano and
  • Davide Slaghenaufi

In winemaking, the use of stems during fermentation is rarely employed due to some undesirable side effects. While the effect of fresh stems on wine is extensively studied, to date limited information is available about the effect of dried stems. Thi...

  • Article
  • Open Access
4 Citations
4,898 Views
12 Pages

Development of Starter Cultures for Precision Fermentation of Kombucha with Enriched Gamma-Aminobutyric Acid (GABA) Content

  • Geun-Hyung Kim,
  • Kwang-Rim Baek,
  • Ga-Eun Lee,
  • Ji-Hyun Lee,
  • Ji-Hyun Moon and
  • Seung-Oh Seo

Kombucha, a fermented tea beverage, is produced through the symbiotic interaction of several microbial strains, including acetic acid bacteria, lactic acid bacteria, and yeast, collectively known as symbiotic culture of bacteria and yeast (SCOBY). As...

  • Review
  • Open Access
15 Citations
5,547 Views
28 Pages

Extremophilic Exopolysaccharides: Bioprocess and Novel Applications in 21st Century

  • Chandni Upadhyaya,
  • Hiren Patel,
  • Ishita Patel and
  • Trushit Upadhyaya

Extremophiles, microorganisms blooming in extreme environmental conditions, hold particular significance in the domain of microbial research. This review paper focuses on extremophilic microorganisms, emphasizing their adaptations and the diverse pro...

  • Review
  • Open Access
78 Citations
30,554 Views
30 Pages

The escalating global demand for nutritious and sustainable food sources has heightened interest in microbial biotransformation in food, a process in which microorganisms chemically modify food components to enhance their functional properties and nu...

  • Article
  • Open Access
3 Citations
2,336 Views
18 Pages

Biotechnological Effects of Lactobacillus plantarum, Cellulase, and Xylanase on Nutritional Quality and Microbial Community Structure of Corn Stover Silage

  • Jianliang Liu,
  • Mingjian Liu,
  • Panjie Sheng,
  • Chaoran Song,
  • Weiqin Ma,
  • Baochao Bai,
  • Jiayu Zhao,
  • Shuai Du,
  • Gentu Ge and
  • Yushan Jia
  • + 1 author

As animal husbandry advances, the demand for premium feed has seen substantial growth, while the availability of natural forage resources remains limited. Corn stover, characterized by its high yield and rich nutritional content, has become a vital s...

  • Article
  • Open Access
2 Citations
1,316 Views
15 Pages

Effects of Different Ratios of Mixed Silage of Licorice Stems and Leaves and Whole Corn on Fermentation Quality, Microbial Diversity, and Aerobic Stability

  • Qifeng Gao,
  • Yuliang Sun,
  • Limin Tang,
  • Haonan Liu,
  • Chenchen Yang,
  • Yulan Ren,
  • Fangpeng Liu,
  • Chunying Jia,
  • Houjun Yu and
  • Tao Jiang

This experiment aimed to investigate the effects of varying proportions of mixed silage on fermentation quality, microbial diversity, and aerobic stability. The mixed silage samples (G10Y0, G9Y1, G8Y2, G7Y3, G6Y4, G5Y5, G4Y6, G3Y7, G2Y8, G1Y9, and G0...

  • Article
  • Open Access
3,654 Views
16 Pages

Extractive Ethanol Fermentation with Ethanol Recovery by Absorption in Open and Closed Systems

  • Kaio César da Silva Rodrigues,
  • Ivan Ilich Kerbauy Veloso,
  • Diego Andrade Lemos,
  • Antonio José Gonçalves Cruz and
  • Alberto Colli Badino

Conventional ethanol production has limitations, including substrate and product inhibitions, which increase both energy requirements for ethanol recovery and vinasse generation. Extractive fermentation, which removes ethanol as it is produced within...

  • Article
  • Open Access
1 Citations
2,124 Views
18 Pages

In the last decade, the production of guinea pig meat in Andean countries has increased due to the growing number of consumers of this meat. Objective: To evaluate the effect of including different doses (0.50, 1.00, and 1.50 mL) of agro-industrial s...

  • Article
  • Open Access
1 Citations
2,147 Views
12 Pages

ε-Poly-L-lysine (ε-PL) is a natural preservative that has excellent properties such as high safety, good antibacterial effect, wide antibacterial spectrum, and high temperature resistance compared to other food preservatives. At prese...

  • Review
  • Open Access
10 Citations
4,874 Views
27 Pages

Redox potential is a solution property that influences specific yeast and bacterial activities and the rate of fermentation completion. There is a need to control it if reproducible fermentation outcomes are to be achieved and reliable conclusions ar...

  • Article
  • Open Access
2 Citations
2,380 Views
23 Pages

Effects of Lactobacillus buchneri and Lactobacillus rhamnosus on Ryegrass Silage Fermentation and Aerobic Stability

  • Furong Han,
  • Mingzhu Zhang,
  • Wentao Sun,
  • Changrong Wu,
  • Yuan Huang,
  • Guanghao Xia,
  • Chao Chen,
  • Fuyu Yang and
  • Jun Hao

Italian ryegrass is a high-quality forage grass, and a full understanding of the changes in its microbiome and metabolome during aerobic exposure can prolong its aerobic stability and improve its utilization value. Italian ryegrass silage was prepare...

  • Article
  • Open Access
3 Citations
2,116 Views
15 Pages

Utilization of Okara as a Culture Medium by Membrane Concentration Process for High Oil Production by Oleaginous Yeast, Lipomyces starkeyi

  • Hiroya Taki,
  • Kentaro Mine,
  • Mana Miyamoto,
  • Jiro Seto,
  • Shinji Matsuo,
  • Kazuo Kumagai and
  • Hideto Matsuyama

Palm oil, widely used in various products, poses environmental and climate change risks. “Yeast oil” produced by Lipomyces starkeyi, an oil-producing yeast, is one of the sustainable alternatives for palm oil and was successfully produced...

  • Article
  • Open Access
2 Citations
3,470 Views
27 Pages

Unveiling the Microbial Symphony of Amasi: A Targeted Metagenomic 16S rRNA, ITS, and Metabolites Insights Using Bovine and Caprine Milk

  • Betty Olusola Ajibade,
  • Titilayo Adenike Ajayeoba,
  • Saheed Sabiu,
  • Konstantin V. Moiseenko,
  • Sizwe Vincent Mbona,
  • Errol D. Cason,
  • Tatyana V. Fedorova and
  • Oluwatosin Ademola Ijabadeniyi

Amasi, a traditional fermented milk produced in Southern Africa, is associated with several health benefits, such as probiotic activities, immune system modulation, and pharmacological (antimicrobial, antitumor and antioxidant) potential. This study...

  • Review
  • Open Access
22 Citations
11,458 Views
20 Pages

Microbial Characteristics and Functions in Coffee Fermentation: A Review

  • Xiaojing Shen,
  • Qi Wang,
  • Hongsheng Wang,
  • Guoqing Fang,
  • Ying Li,
  • Jilai Zhang and
  • Kunyi Liu

Based on coffee’s unique and fascinating flavor, coffee has become the most popular nonalcoholic drink in the world and is a significant agricultural economic crop in tropical- and subtropical-planted coffee countries and regions. It is also be...

  • Article
  • Open Access
2 Citations
2,606 Views
12 Pages

Analysis of a Commercial Red Wine Fermentation Dataset with a Wine Kinetic Model

  • James Nelson,
  • Robert Coleman,
  • Patrick Gravesen,
  • Michael Silacci,
  • Alaina Velasquez,
  • Kimberlee Marinelli and
  • Roger Boulton

The adoption of sensors to monitor wine fermentation enables the collection of large datasets that relate the initial juice chemistry, density and temperature patterns during fermentation to fermentation outcomes. Wine kinetic models are now being ap...

  • Article
  • Open Access
4 Citations
3,126 Views
16 Pages

Sequential Solid-State and Submerged Fermentation to Increase Yarrowia lipolytica Lipase Production from Palm Oil Production Chain By-Products

  • Camila P. L. Souza,
  • Adejanildo da S. Pereira,
  • Érika C. G. Aguieiras and
  • Priscilla F. F. Amaral

This study investigates the potential of sequential solid-state and submerged fermentation (SeqF) to enhance lipase production by Yarrowia lipolytica using by-products from the palm oil production chain. Palm fiber and palm oil deodorizer distillate...

  • Article
  • Open Access
3 Citations
2,213 Views
12 Pages

Optimization of Time-Varying Temperature Profiles for Enhanced Beer Fermentation by Evolutive Algorithms

  • Pablo Ruarte,
  • Nadia Pantano,
  • Marianela Noriega,
  • Cecilia Fernández,
  • Emanuel Serrano and
  • Gustavo Scaglia

Beer is one of the most popular alcoholic beverages globally, leading to continuous efforts to enhance its production methods. Raw materials and the production process are crucial in the brewing industry, with fermentation being a vital stage that si...

  • Communication
  • Open Access
2,146 Views
13 Pages

This study investigates the multifunctional potential of horse oil fermented with barley nuruk, a traditional fermentation starter, focusing on its α-glucosidase inhibitory activity and bioactive applicability. Gas chromatography–tandem m...

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Fermentation - ISSN 2311-5637