Table of Contents
Microorganisms, Volume 7, Issue 1 (January 2019)
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Cover Story (view full-size image) Numerous reports have already suggested that diverse cis- and trans-acting elements including the [...] Read more. Numerous reports have already suggested that diverse cis- and trans-acting elements including the promoter are evolutionarily tuned as a complete set for the expression of a nested open reading frame in integrated gene clusters. Here, we propose that the set of innate elements with a naturally overexpressed gene could be considered as a scaffold for an expression system. For a proof-of-principle study, we minimized the mined expression module from metagenomics DNA by truncation and/or serial deletion analysis and constructed a novel expression system by simple substitution of the target gene with the innately overexpressed ORF in this module. We obtained successful expression of several inserts using this simple construct without tuning and codon optimization of the target insert. This strategy could provide a basis to create a catalogue of alternative gene expression systems and supply the extended part of recombinant protein production.