Special Issue "Recombinant Protein Expression in Microorganisms"
Deadline for manuscript submissions: closed (20 December 2018)
Prof. Dr. Trygve Brautaset
Recombinant protein expression is a fundamental discipline in molecular biology, as well as a key technology to produce any specific protein for scientific and applied purposes. Today, virtually any protein from any origin can be recombinantly overproduced, and microorganisms are in many instances the preferred production hosts. Recombinant expression has been subject for intensive research for decades and yet still today there are fundamental aspects of this science we do not understand. Presumably, critical information is hidden in the gene coding sequences, beyond well-understood rules concerning rare codons and secondary structure formation at the mRNA level. Challenges can be at any level, including host-toxicity, product insolubility and non-functionality, poor translation, and lack of necessary post-translational modification. Genetic tools, superior hosts and new software are being rapidly developed for improved design, by taking all possible parameters (gene coding sequences, promoters, untranslated leaders regions, transcriptional terminators, mRNA stabilities, protein folding, translational efficiencies) into consideration. Still today, recombinant protein expression is largely a matter of trial and error.
In this Special issue of Microorganisms, we invite you to send contributions concerning any aspects related to recombinant protein expression in microorganisms, including genetic tools developments, bioinformatics tools for better predictions of expression levels and protein solubility, methodologies for product detection and characterization, as well as systems biology driven and laboratory-evolution based strategies to better understand and create novel host strains (both eukaryotic and prokaryotic) for improved recombinant protein production.
Prof. Dr. Trygve Brautaset
Prof. Dr. Svein Valla
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Microbial cell factories
- Expression systems
- Protein folding
- Synthetic biology
- Systems biology
- Genetic engineering