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Microbiology Research, Volume 16, Issue 12

2025 December - 18 articles

Cover Story: Members of the genus Kocuria are environmentally versatile Gram-positive bacteria with growing biotechnological relevance. Here, we report the genome-based characterization of Kocuria sp. KH4, isolated from alkaline mine tailings in southeastern Mexico. Phylogenomic analyses and genomic relatedness indices demonstrate that KH4 represents a novel genomospecies. Its genome reveals broad metabolic versatility, extensive metal resistance and homeostasis systems, numerous genomic islands, and multiple biosynthetic gene clusters encoding nonribosomal peptides, polyketides, terpenes, and siderophores. Together, these features highlight the remarkable adaptive capacity of this strain and position Kocuria sp. KH4 as a promising reservoir of new bioactive compounds for environmental, pharmaceutical, and industrial biotechnology. View this paper
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Articles (18)

  • Article
  • Open Access
1 Citations
613 Views
17 Pages

Non- and Medium-Polar Fractions from Trametes villosa Inhibit Staphylococcus aureus Growth, Virulence, and Biofilm Formation

  • Hernando Maldonado-Pérez,
  • Juan Pablo Pinzón-Esquivel,
  • Gloria María Molina-Salinas,
  • Avel Adolfo González-Sánchez,
  • Haziel Eleazar Dzib-Baak,
  • Ángel Dzul-Beh,
  • Carlos Javier Quintal-Novelo and
  • Andrés Humberto Uc-Cachón

Background: Infections caused by Staphylococcus aureus are a health problem worsened by antibiotic resistance. New drugs, including those inhibiting virulence and resistance mechanisms, are needed. This study aimed to evaluate the anti-growth, anti-v...

  • Article
  • Open Access
354 Views
18 Pages

Carbapenem-resistant Enterobacterales (CREs) pose a critical threat to global public health, largely driven by the enzymatic activity of Klebsiella pneumoniae carbapenemase-2 (KPC-2), a class A serine β-lactamase that hydrolyzes most β-lact...

  • Article
  • Open Access
1 Citations
784 Views
17 Pages

Probiotic Potential of Pediococcus acidilactici SWP-CGPA01: Alleviating Antibiotic-Induced Diarrhea and Restoring Hippocampal BDNF

  • You-Zuo Chen,
  • Chieh-Ting Chen,
  • Tsung-Wei Shih,
  • Wei-Hsuan Hsu,
  • Bao-Hong Lee and
  • Tzu-Ming Pan

Gut microbiota dysbiosis is increasingly being recognized as a major contributor to host metabolic imbalance, immune dysfunction, and neurophysiological disorders. Probiotics are known to modulate intestinal metabolism and exert systemic effects thro...

  • Article
  • Open Access
695 Views
14 Pages

Evaluation of the Antibiofilm Activity of Clove Essential Oil in the Development of Bioactive Coatings for Arterial Sampling Devices

  • Ikram Markaoui,
  • Meryem Idrissi Yahyaoui,
  • Abdeslam Asehraou,
  • Abdelkrim Daoudi,
  • Brahim Housni and
  • Houssam Bkiyar

Multidrug-resistant (MDR) pathogens and biofilm-associated infections represent a major global health concern, particularly in the context of medical devices such as catheters, tubing, and blood sampling devices. Biofilms, responsible for up to 85% o...

  • Communication
  • Open Access
422 Views
11 Pages

Enterococcus alishanensis JNUCC 77 (=BLH10) was isolated from the flowers of Zinnia elegans collected at Ilchul Land, Jeju Island, Republic of Korea. Whole-genome sequencing was conducted to clarify its taxonomic position, genomic composition, and ad...

  • Article
  • Open Access
439 Views
13 Pages

Chemical Profiling and Biological Evaluation of Matricaria pubescens as a Promising Source of Antioxidant and Anti-Resistance Agents

  • Elhasnaoui Abdelhadi,
  • Janah Iman,
  • Ait-El-Mokhtar Mohamed,
  • Sellam Khalid,
  • Bouadid Ismail,
  • Eddouks Mohamed,
  • Lahrach Nadia and
  • El-Haidani Ahmed

Matricaria pubescens (Desf.), a medicinal plant belonging to the Asteraceae family, is traditionally used in southeastern Morocco but remains insufficiently studied. In this study, samples collected from the Zagora region (Morocco) were subjected to...

  • Article
  • Open Access
678 Views
11 Pages

Microbiological and Physical–Chemical Quality of Pickled Vegetables Produced by Rural Family Agribusinesses

  • Priscila Endlich Lozer,
  • Rhaiza Marcia Passos Leal and
  • Jackline Freitas Brilhante de São José

The aim of the present study was to assess the microbiological and physical–chemical quality of pickled vegetables produced by rural family agribusinesses in the mountainous region of Espírito Santo State, Brazil. This descriptive, obser...

  • Article
  • Open Access
610 Views
24 Pages

Prospective Yeast Species with Enzymatic, Aromatic, and Antifungal Applications Isolated from Cocoa Fermentation in Various Producing Areas in Côte d’Ivoire

  • Alfred Koffi Yao,
  • Guy Florent Kouamé Amien,
  • Brice Judicaël Assi-Clair,
  • Nabounou Koné,
  • Mai Koumba Koné,
  • Kevin Bethune,
  • Isabelle Maraval,
  • Vincent Chochois,
  • Jean-Christophe Meile and
  • Simplice Tagro Guéhi
  • + 1 author

This research study investigated the potential biotechnological applications of yeast species obtained from cocoa fermentation performed in Côte d’Ivoire. A total of 279 yeast isolates were molecularly identified and then screened for the...

  • Article
  • Open Access
750 Views
18 Pages

Genome Insights into Kocuria sp. KH4, a Metallophilic Bacterium Harboring Multiple Biosynthetic Gene Clusters (BGCs)

  • Gustavo Cuaxinque-Flores,
  • Lorena Jacqueline Gómez-Godínez,
  • Alma Armenta-Medina,
  • Lily X. Zelaya-Molina,
  • Juan Ramos-Garza and
  • José Luis Aguirre-Noyola

The genus Kocuria includes Gram-positive and environmentally versatile bacteria, which are of biotechnological interest due to their ability to synthesize secondary metabolites. In this study, the genome of Kocuria sp. KH4, isolated from alkaline min...

  • Case Report
  • Open Access
534 Views
12 Pages

Visceral leishmaniasis (VL) is a neglected vector-borne disease caused by obligate intracellular protozoa of the genus Leishmania. In immunocompromised patients, VL may present atypically, progress more aggressively, and respond less favorably to tre...

  • Systematic Review
  • Open Access
1,469 Views
22 Pages

Molecular Identification of Escherichia coli Isolated from Street Foods: Global Evidence and Public Health Implications

  • Carmine Fusaro,
  • Natalia Guerrero-Vargas,
  • Yohanna Sarria-Guzmán,
  • Nancy Serrano-Silva,
  • Jaime E. Bernal,
  • Karina Ríos-Montes,
  • Haydee Eliza Romero Luna,
  • Josué Antonio Del Ángel Zumaya,
  • Audry Peredo-Lovillo and
  • Francisco Erik González-Jiménez

Escherichia coli (E. coli) pathotypes present in contaminated food, street food, or water are major contributors to foodborne illnesses. Polymerase chain reaction (PCR) methods are widely applied to detect and confirm E. coli pathotypes in food sampl...

  • Article
  • Open Access
447 Views
17 Pages

Tracing Zoonotic Pathogens Through Surface Water Monitoring: A Case Study in China

  • Yi Wang,
  • Xinyan Du,
  • Xin Du,
  • Liu Yi,
  • Fenglan He and
  • Songzhe Fu

Intensive aquaculture and animal farming along riverbanks have emerged as significant drivers of downstream public health risks, facilitating the transmission of zoonotic pathogens and antimicrobial resistance (AMR) genes from farm effluents into nat...

  • Article
  • Open Access
1 Citations
1,179 Views
21 Pages

Impact of an Essential-Oil-Based Oral Rinse on Oral and Gut Microbiota Diversity: A Pilot Study

  • Elisa Bona,
  • Francesco Cavarra,
  • Alice Caramaschi,
  • Nadia Massa,
  • Chiara Bazzano,
  • Romeo Patini,
  • Vincenzo Rocchetti and
  • Lia Rimondini

Periodontitis is a multifactorial disease that is primarily driven by bacterial biofilm and oral dysbiosis. Listerine® is a widely used essential-oil-based mouthwash that is well established for its safety and anti-plaque efficacy. However, limit...

  • Article
  • Open Access
903 Views
20 Pages

A Situation Analysis of Diagnostic and Management Strategies for Gestational Urinary Tract Infections (UTIs) in Kisumu County, Kenya: Maternal Health Implications and Opportunities for Diagnostic Improvement

  • Shivanthi Samarasinghe,
  • Eunice Namuyenga Toko,
  • Ukpai A. Eze,
  • Esther Furaha,
  • Itodo S. Anthony,
  • Tariq Kapasi,
  • Collins Ouma and
  • Bertha Ochieng

Urinary tract infections (UTIs) are linked to adverse pregnancy outcomes, yet epidemiological data on gestational UTIs in Kenya are limited. This study assessed diagnostic and management practices in Kisumu County to inform diagnostic and antimicrobi...

  • Article
  • Open Access
520 Views
19 Pages

This study aimed to unravel the genomic uniqueness of Streptomyces chrestomyceticus ADP4 using whole-genome sequence analysis, with a focus on identifying strain-specific genes/proteins associated with a novel therapeutic source. The genome of the st...

  • Article
  • Open Access
962 Views
19 Pages

Isolation of Lactic Acid Bacteria from Raw Camel Milk in Saudi Arabia and Evaluation of Their Probiotic Potential

  • Mohammed Alhejaili,
  • Eman Farrag,
  • Sabry Mahmoud,
  • Abd-Ellah Abd-Alla and
  • Tarek Elsharouny

Milk contains wide microbial diversity, composed mainly of lactic acid bacteria (LAB), which are used as probiotics for both humans and livestock. We isolated, characterized, and evaluated LAB from indigenous Saudi Arabian camel milk to assess its pr...

  • Article
  • Open Access
510 Views
15 Pages

4-Nonylphenol (4-NP), an important fine chemical precursor, can cause endocrine disruption, resist natural degradation, and bioconcentrate. Biodegradation is an effective and environmentally sustainable approach to its remediation. This study employe...

  • Article
  • Open Access
548 Views
16 Pages

Evaluation of Purification and Disinfection Potentials of Plant-Based Biomass from Wild Sesame Plant

  • Adeyemi O. Adeeyo,
  • Hlavangwani N. Eulendah,
  • Mercy Alabi,
  • Joshua A. Oyetade,
  • Titus A. M. Msagati and
  • Rachel Makungo

The limitations reported for conventional approaches in the treatment and disinfection of water have led to a recent exploration in the use of plant-based water treatment solutions. This technique leverages natural, renewable, and often locally avail...

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Microbiol. Res. - ISSN 2036-7481