Bio-CO
2 is part of the natural carbon cycle and represents a sustainable carbon source for the production of Renewable Fuels of Non-Biological Origin (RFNBOs), such as synthetic methanol. This study addresses the critical knowledge gap in aligning diverse biogenic CO
2 sources with e-methanol requirements in the EU by providing harmonized mapping, based on datasets, literature sources, and reported industrial statistics at the sectoral and country level. Bio-CO
2 streams from biogas and biogas upgrading, biomass combustion, pulp and paper, bioethanol production, and the food and beverage sector are evaluated for total emissions, CO
2 concentrations and purity, the geographical distribution, seasonality, and impurity profiles. Results show that approximately 350 Mtpa of bio-CO
2 are emitted across the EU, with highly heterogeneous characteristics. Biogas upgrading and fermentation-based processes generate highly pure CO
2 streams (>98–99%), yet their small and dispersed nature complicates logistics. In contrast, biomass-combustion and pulp and paper sectors provide large volumes (around 214.6–298.2 Mtpa and 73.9 Mtpa CO
2, respectively), but in diluted streams (typically 3–15% and 10–20%). Replacing just 10% of the EU maritime fuel demand with e-methanol would require 53.6 Mtpa of bio-CO
2 and 58 GW of electrolyzer capacity, a stark contrast to the current operational 385 MW. The findings highlight the need for infrastructure planning and aggregation hubs to enable the large-scale deployment of RFNBO methanol in the maritime sector.
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