Vaccines

20 pages, 4886 KB

Open AccessArticle

Rv2656c: A Potential Candidate Antigen Associated with Latent Tuberculosis Infection

by Yunjie Du, Pu He, Wenrui Dang, Ting Zhou, Yinjuan Song, Xiaoping Li, Yuhao Zhao, Fei Li, Aizhen Guo and Bingdong Zhu

Vaccines 2026, 14(5), 442; https://doi.org/10.3390/vaccines14050442 - 15 May 2026

Abstract

Background/Objectives: Several subunit vaccines for tuberculosis (TB), such as MVA85A and H4:IC3, have not demonstrated ideal protective efficacy in clinical trials, which may be attributed to their limited antigenic profile and lack of effective latency-associated antigens. In this study, we combined bioinformatics with [...] Read more.

Background/Objectives: Several subunit vaccines for tuberculosis (TB), such as MVA85A and H4:IC3, have not demonstrated ideal protective efficacy in clinical trials, which may be attributed to their limited antigenic profile and lack of effective latency-associated antigens. In this study, we combined bioinformatics with experimental validation to screen for latency-associated antigens that have immune-protective effects. Methods: Highly expressed antigens were identified from models related to latent infections, such as hypoxia and nutritional starvation. Their physicochemical properties and immunogenicity were predicted using online tools such as Expasy-ProParam, IEBD, and VaxiJen. The immunogenicity of these antigens was then evaluated in multiple mycobacterium infection models. Finally, a systematic evaluation of the immune response and protective effects induced by the candidate antigens was performed in a mouse model using intracellular cytokine detection, mycobacterium growth inhibition assays (MGIAs), antibody-dependent cellular phagocytosis (ADCP), and a latent tuberculosis infection (LTBI) mouse model. Results: The antigen Rv2656c is highly expressed in the nutritional starvation model and demonstrates strong immunogenicity in both infected humans and cattle. Moreover, Rv2656c exerted a significant inhibitory effect against Mycobacterium tuberculosis (M. tuberculosis) and Mycobacterium avium (M. avium) infections in MGIA. The humoral immune response elicited by Rv2656c enhanced the phagocytosis and killing of Mycobacteria by macrophages in vitro. Furthermore, in a mouse model of LTBI established using the attenuated M. tuberculosis H37Ra strain, treatment with Rv2656c significantly decreased the bacterial load in the lungs of the mice. Conclusions: Latency-associated Rv2656c may serve as an immune-protective antigen, offering potential for the development of novel multi-stage antigen subunit vaccine against TB. Full article

(This article belongs to the Special Issue Tuberculosis Diagnosis and Vaccines Research)

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13 pages, 982 KB

Open AccessArticle

TTV Viremia and Immune Responses Following Vaccination Against Mpox and Dengue Viruses

by Claudia Minosse, Pietro Giorgio Spezia, Sara Belladonna, Aurora Bettini, Giulia Matusali, Francesca Colavita, Stefania Notari, Linda Petrone, Marta Tiberi, Alessandro Rosario Cavasio, Valentina Mazzotta, Luigi Rosa, Eleonora Cimini, Daniele Focosi, Delia Goletti, Emanuele Nicastri, Andrea Antinori and Fabrizio Maggi

Vaccines 2026, 14(5), 441; https://doi.org/10.3390/vaccines14050441 - 15 May 2026

Abstract

Background: Torquetenovirus (TTV) viremia is increasingly recognized as a biomarker of host immune competence. We assessed the association between baseline TTV DNA levels and immune responses to the Mpox virus (MPXV) and dengue virus (DGV) vaccines in two prospective cohorts. Methods: [...] Read more.

Background: Torquetenovirus (TTV) viremia is increasingly recognized as a biomarker of host immune competence. We assessed the association between baseline TTV DNA levels and immune responses to the Mpox virus (MPXV) and dengue virus (DGV) vaccines in two prospective cohorts. Methods: A total of 248 individuals were enrolled, and TTV DNA was quantified before vaccination. Humoral and cellular responses to MVA-BN (for MPXV) and QDENGA (for DGV) vaccines were measured by using serology, neutralization assays, and interferon-γ ELISpot, and correlations with TTV viremia were investigated. Results: TTV DNA was detected in 81.2% of individuals, with a significantly higher prevalence and viral loads in the Mpox-Vac group than in the DGV-Vac group. Between both groups, the only significant association observed was an inverse correlation between pre-vaccination TTV load and DGV neutralizing antibody titers in the DGV-Vac group and was limited to the subset of TTV-positive individuals; no additional correlations with antibody and T responses were identified. For the Mpox-Vac group, stratified analyses in people living with HIV (PLWH) confirmed this lack of association. Conclusions: TTV viremia does not predict vaccine immunogenicity in immunocompetent or mildly immunosuppressed individuals. These results, which derive from within-cohort analyses and do not rely on direct comparisons between heterogeneous vaccine populations, support the role of TTV as a marker of immune status along a continuum of immunosuppression, with predictive value likely confined to populations with more severe immune impairment. Full article

(This article belongs to the Special Issue Research on Immune Response and Vaccines: 2nd Edition)

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23 pages, 3217 KB

Open AccessReview

Bacterial Membrane Vesicles as Versatile Platforms for Systemic and Mucosal Vaccines

by Si Hyun Park and Young Min Son

Vaccines 2026, 14(5), 440; https://doi.org/10.3390/vaccines14050440 - 15 May 2026

Abstract

Bacterial membrane vesicles (BMVs), encompassing outer membrane vesicles (OMVs) released from Gram-negative bacteria and extracellular vesicles (EVs) released from Gram-positive bacteria, have emerged as promising vaccine platforms owing to their intrinsic immunostimulatory properties and capacity to deliver a wide range of antigens. Although [...] Read more.

Bacterial membrane vesicles (BMVs), encompassing outer membrane vesicles (OMVs) released from Gram-negative bacteria and extracellular vesicles (EVs) released from Gram-positive bacteria, have emerged as promising vaccine platforms owing to their intrinsic immunostimulatory properties and capacity to deliver a wide range of antigens. Although conventional vaccines effectively prevent infectious diseases, their long-term efficacy is often limited by antigenic variation and reliance on a restricted number of licensed adjuvants. BMVs, as self-adjuvanting systems, enable both antigen delivery and innate immune activation. BMVs are nanoscale lipid bilayer structures enriched with pathogen-associated molecular patterns (PAMPs), facilitating their recognition and uptake by antigen-presenting cells. This leads to the activation of pattern recognition receptors and the induction of pro-inflammatory cytokines, type I interferons, and adaptive immune responses, including antibody production and Th1- and Th17-biased cellular immunity. Recent studies highlight the versatility of BMVs as vaccine platforms across bacterial, fungal, and viral infection models. BMVs induce protective immunity by promoting both systemic and mucosal immune responses, thereby reducing bacterial burden and limiting pathogen colonization across diverse infection models. These properties have supported their application in viral vaccine development, including influenza and SARS-CoV-2, with the potential to enhance mucosal immunity. Despite these advantages, challenges remain in standardization, safety, and antigen-loading efficiency. Engineered BMVs incorporating protein or mRNA antigens may further enhance antigen presentation and CD8⁺ T cell responses. This review summarizes the biological features, immunological mechanisms, and future potential of BMVs in vaccine development. Full article

(This article belongs to the Special Issue Vaccine Advancement, Efficacy and Safety: Feature Papers)

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17 pages, 259 KB

Open AccessArticle

Barriers to Childhood Immunisation in Selected Zero-Dose Communities in Gauteng Province: A Qualitative Study

by Thobelani Nompilo Majola, Ntombifuthi Blose, Emma Shuvai Chikovore, Zinhle Mtwane, Algernon Africa, James Michael Burnett, Maanda Mudau, Noluthando Ndlovu, Bontle Motloung, Janine Simon-Meyer and Ashnie Padarath

Vaccines 2026, 14(5), 439; https://doi.org/10.3390/vaccines14050439 - 14 May 2026

Abstract

Background/objective: The Immunisation Agenda 2030, led by the World Health Organization, aims to ensure that people of all ages benefit from vaccination. South Africa remains committed to these goals, strengthening childhood immunisation largely through the Expanded Programme on Immunisation. However, despite progress, [...] Read more.

Background/objective: The Immunisation Agenda 2030, led by the World Health Organization, aims to ensure that people of all ages benefit from vaccination. South Africa remains committed to these goals, strengthening childhood immunisation largely through the Expanded Programme on Immunisation. However, despite progress, the number of unvaccinated and partially vaccinated children continues to rise in some urban settings. This study sought to identify barriers to childhood immunisation in selected zero-dose urban communities in Gauteng Province. Methods: A qualitative exploratory–descriptive design was used to examine factors influencing childhood immunisation. Data were collected through seven focus group discussions and fifteen key informant interviews with purposively selected caregivers, community leaders, community health workers and healthcare workers involved in routine immunisation services at public healthcare facilities across the Cities of Johannesburg, Ekurhuleni, and Tshwane. Thematic analysis was conducted using NVivo^® software. Results: The study identified multiple demand-side and supply-side barriers. Demand-side barriers included limited parental knowledge, lack of trust in the government and immunisation services, religious beliefs, migration, and perceptions of caregiver substance use. Supply-side barriers involved distance to the facility, negative healthcare worker attitudes, long queues and waiting times, and frequent vaccine stock-outs. These barriers collectively contributed to missed opportunities and decreased uptake of immunisation services. Conclusions: Childhood immunisation remains a critical and cost-effective public health intervention. The findings demonstrate the complex interplay of individual and system-level factors influencing vaccine demand, uptake and persistent zero-dose status in urban Gauteng communities. Addressing these barriers requires a comprehensive approach that strengthens community trust, improves caregiver vaccine literacy, and ensures accessible, responsive, and respectful immunisation services. Full article

(This article belongs to the Special Issue Integrating Vaccination/Immunization Services into Universal Health Coverage)

1 pages, 366 KB

Open AccessCorrection

Correction: Watts et al. Multi-Antigen Elephant Endotheliotropic Herpesvirus (EEHV) mRNA Vaccine Induces Humoral and Cell-Mediated Responses in Mice. Vaccines 2024, 12, 1429

by Jessica R. Watts, Jennifer L. Spencer Clinton, Jeroen Pollet, Rongsheng Peng, Jie Tan and Paul D. Ling

Vaccines 2026, 14(5), 438; https://doi.org/10.3390/vaccines14050438 - 14 May 2026

Abstract

The authors would like to make the following corrections to this published paper [...] Full article

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43 pages, 4016 KB

Open AccessSystematic Review

A Possible Unmet Need: Pneumococcal Vaccination in the Workplaces—A Systematic Review of Invasive Pneumococcal Disease Among Shipyard Workers

by Matteo Riccò, Luca Pipitò, Claudio Costantino, Silvio Tafuri, Chiara Noviello, Marco Bottazzoli, Paolo Manzoni, Daniel Fiacchini, Marco Falcone, Pasquale Gianluca Giuri, Davide Gori and Antonio Cascio

Vaccines 2026, 14(5), 437; https://doi.org/10.3390/vaccines14050437 - 13 May 2026

Abstract

Background: Workplace-related outbreaks of invasive pneumococcal disease (IPD) have been increasingly reported among shipyard workers, yet their epidemiological and clinical features remain incompletely characterized. This systematic review and meta-analysis aimed to synthesize available evidence on IPD outbreaks in shipyard settings. Methods: [...] Read more.

Background: Workplace-related outbreaks of invasive pneumococcal disease (IPD) have been increasingly reported among shipyard workers, yet their epidemiological and clinical features remain incompletely characterized. This systematic review and meta-analysis aimed to synthesize available evidence on IPD outbreaks in shipyard settings. Methods: A systematic search of PubMed/MEDLINE, Scopus, EMBASE, and medRxiv was conducted up to March 2026. Observational studies reporting IPD outbreaks in shipyards were included. Pooled incidence rates and clinical outcomes were estimated using random-effects models, with heterogeneity assessed by I² statistics. Risk of bias was evaluated using the Newcastle–Ottawa Scale. Results: Eight studies describing six outbreaks across four European countries (France, Norway, Northern Ireland, Finland; 2015–2025) were included, encompassing 131 cases among 35,623 workers. The pooled incidence was 368.9 cases per 100,000 workers with an attack rate of 2.36 per 1000 person-months for total cases, compared to 200.49 cases per 100,000 workers (95%CI 103.54–387.85) and 1.10 cases per 1000 person-months (95% CI 0.17–2.03) for laboratory confirmed cases, with considerable heterogeneity across studies. Most cases occurred in men (97.7%), with the median age ranging from 39 to 48 years. Hospitalizations occurred in 79.1% of cases, intensive care unit admission in 13.7%, and the case fatality ratio was 0.8%. Serotype 4 accounted for 67.2% of characterized isolates. Occupational exposures and shared accommodation may have contributed to transmission, although this could not be formally assessed. Conclusions: IPD outbreaks in shipyard settings are characterized by high incidence but relatively favorable outcomes, likely reflecting workforce demographics. However, considerable heterogeneity and methodological limitations across studies constrain the interpretation of pooled estimates. Preventive strategies, including vaccination and workplace-targeted interventions, should be considered as plausible public health measures, with a proactive role for occupational health services. Full article

24 pages, 8636 KB

Open AccessArticle

Enhanced Anti-Lung Cancer Efficacy of Neo-BCV Combined with Cisplatin: Immune Activation and Tumor Microenvironment Remodeling

by Quexun Cai, Qianli Yang, Kangrui Zhang, Zhengyue Fei, Ruochen Zhao, Tao Lu, Kecheng Xu, Zhenyi Wang and Peihua Lu

Vaccines 2026, 14(5), 436; https://doi.org/10.3390/vaccines14050436 - 13 May 2026

Abstract

Background: Lung cancer is the top cause of cancer-related mortality globally, and chemo-immunotherapy is a core therapeutic strategy for it. The novel bacterial composite vaccine (Neo-BCV) we developed previously can activate anti-tumor immunity. This study explored its synergistic anti-tumor effect with cisplatin (CDDP), [...] Read more.

Background: Lung cancer is the top cause of cancer-related mortality globally, and chemo-immunotherapy is a core therapeutic strategy for it. The novel bacterial composite vaccine (Neo-BCV) we developed previously can activate anti-tumor immunity. This study explored its synergistic anti-tumor effect with cisplatin (CDDP), along with the underlying immunomodulatory mechanisms and molecular regulatory networks. Methods: A murine Lewis lung cancer (LLC) model was established to evaluate the efficacy of the combination therapy. Flow cytometry and multiplex cytokine assay were used to detect immune cell subsets and functional molecules in the spleen, serum and tumor tissues. RNA-sequencing (RNA-seq) was used to elucidate the molecular regulatory networks following the combination therapy in the tumor tissues. Body weight, blood indexes, serum biochemistry and H&E staining were monitored to verify biosafety. Results: Neo-BCV combined with CDDP achieved an 87.77% tumor growth inhibition rate, showing the most significant anti-tumor effect. The combination promoted DC maturation, enhanced effector immune cell infiltration, reduced immunosuppressive cells, upregulated Th1-type cytokines and downregulated CD8⁺ T cell surface PD-1. RNA-seq confirmed enrichment of multiple immune effector pathways, supporting tumor immune microenvironment remodeling. The combination alleviated CDDP-induced weight loss, had no obvious adverse effects on physiological indicators, and exhibited good biosafety. Conclusions: Neo-BCV combined with CDDP achieves enhanced anti-tumor efficacy and favorable biosafety in murine lung cancer models by regulating immune cell subsets and activating immune-related molecular pathways, providing a solid preclinical basis for its clinical translation in lung cancer treatment. Full article

(This article belongs to the Section Vaccination Against Cancer and Chronic Diseases)

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22 pages, 858 KB

Open AccessReview

Immune Cell Signaling in Feline Infectious Peritonitis Virus Infection and Implications for Vaccine Design

by Hye-Mi Lee

Vaccines 2026, 14(5), 435; https://doi.org/10.3390/vaccines14050435 - 13 May 2026

Abstract

Feline infectious peritonitis virus (FIPV) remains one of the most challenging viral diseases in veterinary medicine, largely owing to the absence of a consistently effective and safe vaccine. Despite widespread feline coronavirus infection, only a subset of infected cats progresses to feline infectious [...] Read more.

Feline infectious peritonitis virus (FIPV) remains one of the most challenging viral diseases in veterinary medicine, largely owing to the absence of a consistently effective and safe vaccine. Despite widespread feline coronavirus infection, only a subset of infected cats progresses to feline infectious peritonitis, indicating that host immune responses are key determinants of disease outcomes. Accumulating evidence indicates that disease severity is driven not only by viral replication but also by macrophage- and monocyte-centered immune signaling, leading to excessive inflammation and systemic immunopathology in the host. Previous vaccine approaches against FIPV have failed to provide consistent protection and, in some cases, have been associated with enhanced disease. These outcomes suggest that vaccine-induced immune responses that recapitulate pathogenic signaling patterns may exacerbate disease rather than confer protection. In this review, we discuss the current knowledge of immune cell signaling pathways implicated in FIPV infection, including innate sensing through Toll-like receptors, downstream mitogen-activated protein kinases and NF-κB signaling, cytokine production profiles, Fc receptor-associated processes, and intracellular pathways such as autophagy, and how these mechanisms shape vaccine-induced immunity. By integrating insights from immune signaling kinetics, antibody functionality, adjuvant-driven pathway engagement, and platform-specific immune signatures, this review emphasizes the need to reframe FIPV vaccine development strategies that actively shape host immune responses. Rather than maximizing immunogenicity, successful vaccine design is likely to depend on limiting sustained macrophage activation and pro-inflammatory cytokine amplification while supporting antiviral immune functions, thereby reducing the risk of antibody-dependent enhancement and immunopathology. Beyond feline diseases, these considerations provide broader lessons for vaccine design in settings where immune-mediated pathology contributes to disease severity. Full article

(This article belongs to the Section Pathogens-Host Immune Boundaries)

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3 pages, 136 KB

Open AccessEditorial

Closing Editorial—Special Issue on “Vaccines and Vaccination: HIV, Hepatitis Viruses and HPV”

by Sonia Moretti and Ivan Schietroma

Vaccines 2026, 14(5), 434; https://doi.org/10.3390/vaccines14050434 - 12 May 2026

Abstract

We are pleased to present this closing editorial for the Special Issue “Vaccines and Vaccination: HIV, Hepatitis Viruses and HPV”, which we had the privilege of coordinating [...] Full article

(This article belongs to the Special Issue Vaccines and Vaccination: HIV, Hepatitis Viruses, and HPV)

1 pages, 123 KB

Open AccessCorrection

Correction: Loayza et al. Monitoring the Performance of National Immunization Programs: Innovative Methodology and Tool for Countries’ Self-Assessment. Vaccines 2026, 14, 258

by Sergio Loayza, Bertha Capistran, Marcela Contreras, Martha Velandia and Daniel Salas

Vaccines 2026, 14(5), 433; https://doi.org/10.3390/vaccines14050433 - 12 May 2026

Abstract

The authors would like to make the following correction to this published paper [...] Full article

(This article belongs to the Section Vaccines and Public Health)

16 pages, 1116 KB

Open AccessReview

Impact of the COVID-19 Pandemic on HPV Vaccination in Low- and Middle-Income Countries: A Scoping Review

by Joyce Omondi, Robert Ambogo, Candy Ochieng, Marwa Farag and George Mutwiri

Vaccines 2026, 14(5), 432; https://doi.org/10.3390/vaccines14050432 - 12 May 2026

Abstract

Background: The COVID-19 pandemic caused disruptions in HPV vaccination and may have severely undermined global cervical cancer prevention, posing long-term risks to controlling cervical cancer and other HPV-related diseases. Objective: We conducted a scoping review to map and synthesize available evidence on how [...] Read more.

Background: The COVID-19 pandemic caused disruptions in HPV vaccination and may have severely undermined global cervical cancer prevention, posing long-term risks to controlling cervical cancer and other HPV-related diseases. Objective: We conducted a scoping review to map and synthesize available evidence on how the COVID-19 pandemic has affected human papillomavirus (HPV) vaccination programs in low- and middle-income countries (LMICs) focusing on changes in vaccine delivery and coverage, determinants of uptake, economic and programmatic consequences and vaccine hesitancy. Methods: Inclusion criteria were limited to studies published in the English language between January 2020 to May 2025, and followed JBI and Arksey & O’Malley’s scoping review guidelines. The review proceeded through three stages: database searches, gray literature and citation tracking and used a PRISMA-ScR checklist to guide narrative and tabular synthesis. Results: A total of 1063 records, 57 studies were included in the final analysis, and these were spread out across 37 low- and middle-income countries (LMICs) mainly in Africa, Asia, and Latin America. Our analysis revealed that HPV vaccination coverage declined substantially during the COVID-19 pandemic, with reductions of up to 90% reported across the included studies, in the context of school closures, workforce redeployment, and supply-chain disruptions. Recovery efforts also faced major barriers including vaccine hesitancy, misinformation about COVID-19 vaccines, and travel restrictions. Strategies like digital tools, mobile clinics, and community health workers showed promise alongside integrated school- and facility-based approaches, although there is limited evidence on cost-effectiveness and long-term sustainability of these strategies. Conclusions: HPV vaccination in LMICs was significantly disrupted by the COVID-19 pandemic due to unreliable vaccine supply chains, health-worker shortages, and challenges tied to school-based vaccine delivery. Although recovery methods show potential, longer observation periods are needed to determine their full effectiveness. Full article

(This article belongs to the Special Issue Strategies to Increase the Uptake and Delivery of HPV Vaccination in LMICs)

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10 pages, 222 KB

Open AccessArticle

Support for Tying Polio Vaccination Status to Child Tax Credit Eligibility in the United States

by Matthew R. Boyce and Rebecca Katz

Vaccines 2026, 14(5), 431; https://doi.org/10.3390/vaccines14050431 - 12 May 2026

Abstract

Background/Objectives: In a context characterized by persistent vaccine hesitancy, shifting mandates, and stagnating immunization coverage rates, novel policy tools may be required to bolster immunization coverage in the United States. We conducted a national survey to characterize public support for a new tax [...] Read more.

Background/Objectives: In a context characterized by persistent vaccine hesitancy, shifting mandates, and stagnating immunization coverage rates, novel policy tools may be required to bolster immunization coverage in the United States. We conducted a national survey to characterize public support for a new tax policy that would require parents to prove that their children are age-appropriately immunized against polio to be eligible for the federal child tax credit. Methods: The survey was conducted in November 2025. Respondents were asked to provide demographic information and use a Likert-scale to indicate their support for the proposed policy. Chi-squared tests and ordinal logistic regression models were used to compare support for the proposed policy across subpopulations. Results: 980 individuals were included in the analysis. 55.8% of respondents supported adding age-appropriate polio immunization to the child tax credit eligibility criteria. 20.9% of respondents opposed the policy proposal. Relative levels of support for the policy differed according to respondent gender, age, 2024 presidential election behavior, and geographic region. However, support did not differ significantly according to race, ethnicity, educational attainment, income, or partisanship. Conclusions: Results show that most survey respondents would support a policy that would add polio immunization status to the eligibility criteria for the federal child tax credit. Further, support did not differ across key demographic and political subgroups. Larger surveys should validate these findings and investigate support for adding additional vaccines to the eligibility criteria. Full article

(This article belongs to the Special Issue New Challenges in Vaccines and Vaccination: A Public Health Perspective)

15 pages, 1274 KB

Open AccessArticle

The Yellow Fever Virus Type-Specific Epitope Recognized by Monoclonal Antibody 2D12 Neutralizes Wild Type, but Not Live Attenuated 17D or French Neurotropic Vaccine Strains

by Clairissa A. Hansen, Shawn Rast, Jill K. Thompson, Haiping Hao, Daniel Jupiter, Stephen Higgs, Nigel Bourne and Alan D. T. Barrett

Vaccines 2026, 14(5), 430; https://doi.org/10.3390/vaccines14050430 - 12 May 2026

Abstract

Background/Objectives: The envelope (E) protein of orthoflaviviruses contains antigenic sites that are composed of one or more epitopes, which can vary in antigenic specificity, including between viral species, strains, and even substrains. Monoclonal antibodies (mAbs) that bind these epitopes vary in functionality [...] Read more.

Background/Objectives: The envelope (E) protein of orthoflaviviruses contains antigenic sites that are composed of one or more epitopes, which can vary in antigenic specificity, including between viral species, strains, and even substrains. Monoclonal antibodies (mAbs) that bind these epitopes vary in functionality based on their specificity. This makes mAbs useful to study the differences in phenotypes between strains of viruses, such as the wild type (WT) and live attenuated vaccine strains of yellow fever virus (YFV). mAb 2D12 was raised against the 17D-204 YFV vaccine substrain virus (YF VAX^®) by Schlesinger et al. in 1983. However, it only neutralizes Asibi WT virus, not the 17D-204 vaccine substrain virus. Results: We confirmed these results and demonstrated that mAb 2D12 fails to neutralize all 17D vaccine substrains (17D-204, 17DD, and 17D-213), indicating that the minor differences between these virus substrains do not affect the epitope or functionality of mAb 2D12. In addition, mAb 2D12 was found to neutralize WT strain of French viscerotropic virus (FVV), with statistically indistinguishable neutralization from the WT strain Asibi. All but one of the live attenuated French neurotropic vaccine (FNV) derivative viruses had significantly lower neutralization than WT strains Asibi and FVV. FVV, Asibi, 17D, and FNV have many amino acid differences in the membrane (M) and E proteins. It is unclear which of them contributes to this differential neutralization. However, FNV and 17D have common amino acid substitutions from WT FVV and Asibi at positions M-36 and E-331, suggesting that one or both of these residues may contribute to the 2D12 epitope. Conclusions: Overall, mAb 2D12 is a valuable tool to distinguish WT virulent strains of YFV from live attenuated vaccine strains. Full article

(This article belongs to the Special Issue Arboviral Infections (Dengue, Chikungunya, Zika, and Other Mosquito-Borne Diseases): Immune Response and Vaccine Development)

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15 pages, 1295 KB

Open AccessArticle

Determinants and Dynamics of COVID-19 Vaccine Hesitancy in University Students: A Machine Learning Analysis

by Daliana Lobo Torres and Zahid Ahmad Butt

Vaccines 2026, 14(5), 429; https://doi.org/10.3390/vaccines14050429 - 11 May 2026

Abstract

Background: Booster vaccine hesitancy poses a challenge to sustained COVID-19 immunization even among individuals who accepted primary vaccination. This study examined associated factors and patterns of change in vaccine attitudes among university students in Ontario, Canada. Methods: A cross-sectional survey dataset was analyzed [...] Read more.

Background: Booster vaccine hesitancy poses a challenge to sustained COVID-19 immunization even among individuals who accepted primary vaccination. This study examined associated factors and patterns of change in vaccine attitudes among university students in Ontario, Canada. Methods: A cross-sectional survey dataset was analyzed using validated psychometric scales to measure hesitancy toward primary and booster COVID-19 vaccination. Changes in hesitancy were operationalized as the continuous difference between booster and primary scores (

Δ V H

). Gradient Boosting and XGBoost regression models were fitted to estimate

Δ V H

from demographic characteristics (age, gender, socioeconomic status), vaccination history, and attitudinal constructs including complacency, confidence in vaccine safety, and perceived necessity of vaccination. Predictor contributions were assessed using SHapley Additive exPlanations, and Gaussian Mixture Modeling was employed to identify latent profiles among students with increased hesitancy. Results: A substantial proportion of students demonstrated higher hesitancy toward booster doses. Attitudinal factors, particularly complacency and safety perceptions, were the most influential predictors of increased hesitancy, whereas sociodemographic characteristics showed limited influence. Three distinct profiles of booster hesitancy were identified, reflecting heterogeneous patterns of vaccine attitudes and behaviors. Conclusions: These findings suggest that booster hesitancy in the study population is primarily associated with modifiable perceptions and can be effectively characterized using machine learning approaches that may inform targeted public health communication strategies. Full article

(This article belongs to the Special Issue Acceptance and Hesitancy in Vaccine Uptake: 3rd Edition)

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22 pages, 2973 KB

Open AccessArticle

Mpox Vaccination Willingness, Concern Profiles, and Associated Factors Among Men Who Have Sex with Men in Changsha, China

by Yingying Zhou, Wenqiang Wang, Yun Kuang, Qiang Hu, Lin Shen, Qiangming Xie and Zhi Xie

Vaccines 2026, 14(5), 428; https://doi.org/10.3390/vaccines14050428 - 10 May 2026

Abstract

Background: Mpox vaccination is an important prevention strategy for men who have sex with men (MSM), yet responses to vaccination may not be adequately captured by a simple willing-versus-unwilling framing. We examined correlates of vaccination willingness and heterogeneity within the delayed/refused responses [...] Read more.

Background: Mpox vaccination is an important prevention strategy for men who have sex with men (MSM), yet responses to vaccination may not be adequately captured by a simple willing-versus-unwilling framing. We examined correlates of vaccination willingness and heterogeneity within the delayed/refused responses subgroup in Changsha, China. Methods: A cross-sectional survey was conducted using respondent-driven sampling (RDS). Vaccination willingness was classified as immediate willingness versus delayed/refused responses. Analyses included cluster-robust logistic regression, RDS-weighted regression, generalized estimating equations, and a recruiter-linked network-lag model. Among respondents with delayed/refused responses, concern profiles were explored using unsupervised clustering of standardized concern items. Results: Among 405 recruited MSM without a self-reported mpox infection history, immediate willingness and delayed/refused responses were nearly equally distributed, indicating that lack of immediate willingness was common. Across primary models, ever use of pre-exposure prophylaxis (PrEP) and higher mpox-related information exposure frequency were the most consistent correlates of immediate willingness versus delayed/refused responses, whereas basic sociodemographic variables showed little evidence of independent association. Within the delayed/refused group, three partially overlapping patterns emerged: broadly elevated cross-domain concern, low-concern delay with few strongly endorsed barriers, and more selective safety- and burden-related concerns. These findings suggest that a lack of immediate willingness may arise through different psychosocial pathways rather than a single common mechanism. Conclusions: Mpox vaccination willingness among MSM in Changsha appeared to be shaped more by prevention-related behaviors and psychosocial factors than by basic sociodemographic profiles alone. Vaccination strategies may benefit from cross-topic sexual-health communication, integrated prevention efforts, and subgroup-sensitive approaches to delayed or refused willingness. Full article

(This article belongs to the Special Issue Epidemiology and Vaccinations in Infectious Diseases)

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22 pages, 1450 KB

Open AccessArticle

Comparison Between a Minimally Invasive Capillary Blood Sampling Technique and Venipuncture for Core Vaccine Antibody Titration in Dogs

by Paola Dall‘Ara, Lucia Tamanza, Federico Alghisi, Davide Raccagni, Alice Garegnani and Joel Filipe

Vaccines 2026, 14(5), 427; https://doi.org/10.3390/vaccines14050427 - 10 May 2026

Abstract

Background/Objectives: International guidelines advocate for personalized vaccination protocols using point-of-care (POC) antibody titration to identify dogs requiring boosters for CPV-2, CDV, and CAdV-1. As traditional venipuncture can be challenging in specific patients, this study evaluate the clinical agreement of a novel minimally [...] Read more.

Background/Objectives: International guidelines advocate for personalized vaccination protocols using point-of-care (POC) antibody titration to identify dogs requiring boosters for CPV-2, CDV, and CAdV-1. As traditional venipuncture can be challenging in specific patients, this study evaluate the clinical agreement of a novel minimally invasive capillary blood sampling technique (ear-prick) for core vaccine antibody titration. Methods: Paired blood samples were collected from 55 healthy dogs using venipuncture and an ear-prick technique with a portable lancet. Antibody titers were determined using a semi-quantitative POC kit (VacciCheck^® Canine). The procedure was optimized comparing 28G and 21G lancets, with the latter used in 43 dogs to ensure adequate blood flow. Comprehensive statistical methods evaluated the correlation and agreement between the two sampling techniques. Results: Statistical analysis showed no significant differences between sampling methods (p > 0.05). In the optimized group (21G lancet), full match rates reached 81.4% for CPV-2, 76.8% for CDV, and 74.4% for CAdV-1. Clinical concordance was exceptionally high: 95.3% for CPV-2, 90.7% for CDV, and 100% for CAdV-1. Statistical analysis confirmed perfect agreement (1.00) for CPV-2 and CAdV-1, and moderate agreement (0.48) for CDV. Conclusions: The ear-prick technique using a 21G lancet is a reliable, minimally invasive alternative to venipuncture for antibody titration. This method simplifies clinical procedures and facilitates personalized immunization monitoring. Given the minimal blood volume required, it represents a versatile approach for evaluating immune status and protection levels to core vaccines in diverse settings, including pediatric and shelter medicine. Full article

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4 pages, 146 KB

Open AccessEditorial

Vaccination in Immune-Mediated Intestinal Diseases: Efficacy, Safety, and Future Directions

by Adi Lahat and Kassem Sharif

Vaccines 2026, 14(5), 426; https://doi.org/10.3390/vaccines14050426 - 9 May 2026

Abstract

Vaccination remains one of the most effective public health interventions for preventing infectious diseases, reducing morbidity, mortality, and healthcare burden worldwide [...] Full article

(This article belongs to the Special Issue Vaccine Efficacy and Safety in Patients with Immune-Mediated Intestinal Diseases)

27 pages, 4626 KB

Open AccessArticle

A Human Mobility-Based Modeling Study of Influenza Vaccination Strategies Across Socioeconomically Disparate Regions in China

by Lei Zhou, Yunkang Zhao, Hongjie Wei, Kang Fang, Huimin Qu, Yanshu Ke, Jia Rui, Dayan Wang, Tianmu Chen and Liming Li

Vaccines 2026, 14(5), 425; https://doi.org/10.3390/vaccines14050425 - 9 May 2026

Abstract

Background: China‘s influenza vaccination coverage remains at a low rate, with significant regional socioeconomic disparities, lacking targeted distribution strategies and achievable coverage targets. This study aims to provide scientific evidence for formulating differentiated and feasible vaccination strategies across Chinese provinces based on regional [...] Read more.

Background: China‘s influenza vaccination coverage remains at a low rate, with significant regional socioeconomic disparities, lacking targeted distribution strategies and achievable coverage targets. This study aims to provide scientific evidence for formulating differentiated and feasible vaccination strategies across Chinese provinces based on regional economic gradients. Methods: We employed the Susceptible-Vaccinated-Exposed-Asymptomatic-Infectious-Critical-Fatal-Recovered/Removed (SVEAICFR) model to simulate various vaccination strategies, analyzing the reduction in disease burden and vaccine dose requirements across underdeveloped, developing, and developed regions. The optimal strategy and achievable coverage targets were subsequently determined. Results: The 31 provinces were clustered into three categories based on economic levels, showing significant spatiotemporal differences in epidemics (Kruskal–Wallis test, all p < 0.001). Developed regions showed the earliest onset and highest peaks (influenza-like illness positive (ILI+) index ≈ 12–13, Baidu Influenza Search Index (BISI) ≈ 310,000). Developing regions exhibited moderate lagging by 1–2 weeks, while underdeveloped regions had the lowest peaks (ILI+ 3–4) and longer epidemic cycles. During the 2023–2024 influenza season, the national predicted vaccination rate was only 2.89% with marked regional disparities. Baseline incidence, severity, and mortality rates were 13,374.93, 49.52, and 8.37 cases per 100,000 population, respectively. Modeling indicates that increasing influenza vaccination coverage rates for populations aged <18 and ≥65 to a theoretical threshold (39.73% of the total population) before the season could reduce incidence, severity, and mortality rate by 99.26%,99.42%, and 99.46%, respectively. Conclusions: Influenza prevalence in China exhibits significant regional heterogeneity, necessitating differentiated measures based on regional economic gradients. Regional support mechanisms should be implemented to promote equitable vaccine distribution. Priority vaccination for high-risk populations (aged <18 and ≥65), to reach a 40% theoretical national coverage target, is recommended via realistic implementation pathways to minimize the disease burden of influenza. Full article

(This article belongs to the Special Issue New Technology for Vaccines and Vaccine-Preventable Diseases)

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Open AccessArticle

Incidence of COVID-19 and Influenza-Related Outcomes and Vaccinations in the United States, October 2022 Through December 2024

by Heather R. Hensler, Tianyi Lu, Yoonyoung Park, Machaon Bonafede, Isabelle Winer, Christopher Adams, Keya Joshi and Amanda Wilson

Vaccines 2026, 14(5), 424; https://doi.org/10.3390/vaccines14050424 - 8 May 2026

Abstract

Background/Objectives: We still do not clearly know whether COVID-19 continues to impose a greater clinical burden than influenza in the “post-pandemic” era. Our study quantified and compared monthly COVID-19 and influenza hospitalization incidence among adult subgroups from October 2022 through December 2024. We [...] Read more.

Background/Objectives: We still do not clearly know whether COVID-19 continues to impose a greater clinical burden than influenza in the “post-pandemic” era. Our study quantified and compared monthly COVID-19 and influenza hospitalization incidence among adult subgroups from October 2022 through December 2024. We assessed vaccine coverage trends and examined vaccination status among those hospitalized. Methods: Using the Veradigm linked claims and electronic health record dataset, we conducted a non-interventional, retrospective cohort study; three monthly cohorts included individuals aged 65+, high-risk (HR) adults (defined as adults 18+ with HR conditions and/or aged 65+), and adults aged 50–64 years who were enrolled with both medical and pharmacy coverage. We estimated monthly cumulative incidence of COVID-19 and influenza-related hospitalizations, vaccination coverage rates, and the proportion of hospitalized individuals who had received yearly updated vaccines. Results: COVID-19 hospitalizations consistently exceeded those of influenza across months and populations. Among adults aged 65+, COVID-19 hospitalization rates were 2–3 times higher than influenza in winter and 20–30 times higher during off-season months, with similar trends observed in high risk adults. COVID-19 incidence surged in summer, while influenza remained seasonally confined. Vaccination coverage for influenza peaked near 50% annually; COVID-19 coverage was lower, peaking at ~26% by December each year. Most hospitalizations occurred among unvaccinated individuals, particularly for COVID-19. Conclusions: COVID-19 continues to impose a substantial, year-round burden, particularly in older and high-risk adults, exceeding that of influenza. The high proportion of unvaccinated hospitalizations highlight a critical gap in prevention efforts and underscore the need for improved public health messaging and vaccine adoption. Full article

(This article belongs to the Section COVID-19 Vaccines and Vaccination)

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