Special Issue "Plant Tissue Culture"

A special issue of Plants (ISSN 2223-7747).

Deadline for manuscript submissions: 31 October 2020.

Special Issue Editor

Dr. Iyyakkannu Sivanesan
E-Mail Website
Guest Editor
Konkuk University
Interests: plant tissue culture; secondary metabolites; hydroponics

Special Issue Information

Dear Colleagues,

Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge gained towards harnessing more from plants. It is an important technique that involves growing cells, tissues, and organs of plants on artificial media under a controlled environment. Several in vitro culture methods have been used to study and improve our knowledge on basic and advanced areas of plant biology, such as biochemistry, cytology, embryology, molecular biology, and physiology. This Special Issue is aimed at covering various aspects of plant tissue culture, such as germplasm conservation, genetic manipulation, morphogenesis, somatic embryogenesis, nutrition, large-scale clonal propagation, and the production of disease-free plants and useful metabolites.

Dr. Iyyakkannu Sivanesan
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • plant regeneration
  • shoot proliferation
  • somatic embryogenesis
  • germplasm conservation
  • genetic manipulation
  • clonal propagation
  • bioactive compounds
  • virus-free plants
  • cell suspension culture
  • hairy root culture

Published Papers (1 paper)

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Open AccessArticle
Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica
Plants 2019, 8(12), 558; https://doi.org/10.3390/plants8120558 - 29 Nov 2019
In vitro regeneration is a pre-requisite for developing transgenic plants through tissue culture-based genetic engineering approaches. Huge variations among different genotypes of the genus Brassica necessitate the identification of a set of regeneration conditions for a genotype, which can be reliably used in [...] Read more.
In vitro regeneration is a pre-requisite for developing transgenic plants through tissue culture-based genetic engineering approaches. Huge variations among different genotypes of the genus Brassica necessitate the identification of a set of regeneration conditions for a genotype, which can be reliably used in transformation experiments. In this study, we evaluated the morphogenesis potential of four commercial cultivars (Faisal canola, Punjab canola, Aari canola, Nifa Gold) and one model, Westar, from four different explants namely cotyledons, hypocotyls, petioles and roots on three different Brassica regeneration protocols, BRP-I, -II and -III. The regeneration efficiency was observed in the range of 6–73%, 4–79.3%, 0–50.6%, and 0–42.6% from cotyledons, petioles, hypocotyls and roots, respectively, whereas, the regeneration response in terms of average shoots per explant was found to be 0.76–10.9, 0.2–3.2, 0–3.4 and 0–2.7 from these explants. Of the commercial varieties tested, almost all varieties showed poorer regeneration than Westar except Aari canola. In comparison to Westar, its regeneration frequency from cotyledons was up to 7.5-fold higher on BRP-I, while it produced up to 21.9-fold more shoots per explant. Our data show that the explant has strong influence on the regeneration response, ranging from 24% to 92%. While the growth of commercial cultivars was least affected by the regeneration conditions provided, the effect on Westar was twice that of the commercial cultivars. After determining the optimal explant type and regeneration conditions, we also determined the minimum kanamycin concentration levels required to selectively inhibit the growth of untransformed cells for these cultivars. Regenerated shoots of Aari canola could be successfully grown to maturity within 16–18 weeks, with no altered phenotype noted and normal seed yields obtained. Therefore, the commercial variety, Aari canola, could be a good candidate for future genetic transformation studies. Full article
(This article belongs to the Special Issue Plant Tissue Culture)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

1) Chemical profile, phenolic compounds and antioxidant activity of Lychnis flos-cuculi L. in vitro cultures
(Michał Maliński, Małgorzata Kikowska, Barbara Thiem, Anna Soluch, Anna Stochmal, Mariusz Kowalczyk, Michalina Jaworska, Monika Derda, Edward Hadaś) 
2) Micropropagation of endangered plant Linneae borealis L. as a dual strategy for its ex situ conservation and alternative source of polyphenols
(Barbara Thiem, Natalia Turowska, Małgorzata Kikowska, Elwira Sliwinska, Dariusz Kruszka, Justyna Chanaj-Kaczmarek, Piotr Kachlicki)
3) Micropropagation and somatic seeds production of endangered plant Eryngium alpinum L. 
(Małgorzata Kikowska, Barbara Thiem, Elwira Sliwinska)
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