Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica
Abstract
:1. Introduction
2. Results
2.1. Shoot Regeneration from Cotyledons
2.2. Shoot Regeneration from Detached Petioles
2.3. Shoot Regeneration from Hypocotyls
2.4. Shoot Regeneration from Roots
2.5. Effect of Explant, Regeneration Conditions, and Their Interaction on In Vitro Regeneration
2.6. Determining Kanamycin Sensitivity Levels
2.7. Completion of Regeneration Cycle for Aari canola
2.8. Rooting Efficiency
3. Discussion
4. Materials and Methods
4.1. Plant Material and Growth Conditions
4.2. Sterilization and Sowing
4.3. Explant Isolation and Shoot Induction
4.4. Kanamycin Selection Levels
4.5. Growing In Vitro Regenerated Shoots to Maturity
4.6. Rooting Efficiency
4.7. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Source of Variation | DF | Faisal Canola | Aari Canola | Nifa Gold | Punjab Canola | Westar |
---|---|---|---|---|---|---|
Explant (E) | 3 | 6552 **** | 368,487 **** | 26,280 *** | 6644 **** | 10,035 **** |
Regeneration Conditions (RC) | 2 | 8297 *** | 11,116 *** | 1026 NS | 8261 **** | 35,692 ** |
Interaction (E x RC) | 6 | 2540 **** | 9938 **** | 1762 NS | 3202 **** | 2323 ** |
Replicate | 8 | 115.9 NS | 795.6 NS | 891.1 NS | 318.7 *** | 302.9 NS |
Residual Error | 16 | 236.1 | 596.7 | 769.1 | 180.1 | 432.5 |
Source of Variation | Percent Contribution of a Treatment to the Total Experimental Variations | ||||
---|---|---|---|---|---|
Faisal canola | Aari canola | Nifa Gold | Punjab canola | Westar | |
Explant Type (E) | 34.98 | 91.87 | 71.09 | 32.62 | 24.13 |
Regeneration Conditions (RC) | 29.53 | 1.848 | 1.850 | 27.04 | 57.21 |
Interaction (E x RC) | 27.12 | 4.956 | 9.533 | 31.44 | 11.17 |
Replicate | 1.650 | 0.529 | 6.428 | 4.173 | 1.942 |
Residual Error | 6.720 | 0.797 | 11.09 | 4.727 | 5.548 |
Total | 100.0 | 100.0 | 100.0 | 100.0 | 100.0 |
Sr. No. | Cultivar | Species | Source |
---|---|---|---|
1 | Aari canola | Brassica juncea | Oilseed Research Institute, Ayyub Agriculture Research Institute (AARI), Faisalabad, Pakistan |
2 | Faisal canola | Brassica napus | Oilseed Research Institute, Ayyub Agriculture Research Institute (AARI), Faisalabad, Pakistan |
3 | Punjab canola | Brassica napus | Oilseed Research Institute, Ayyub Agriculture Research Institute (AARI), Faisalabad, Pakistan |
4 | Nifa Gold | Brassica napus | Nuclear Institute for Food and Agriculture, Peshawar, Pakistan |
5 | Westar | Brassica napus | National Oilseed Development Program (NODP), National Agricultural Research Centre (NARC), Islamabad, Pakistan |
Reagents | BRP-I | BRP-II | BRP-III | ||||||
---|---|---|---|---|---|---|---|---|---|
SIM | GRM | CIM | SIM | RIM | CIM | SIM | SOM | RIM | |
MS salts (mg/L) | 4.43 | 4.43 | 4.43 | 4.43 | 4.43 | 4.43 | 4.43 | 2.215 | |
Gamborg’s salts (mg/L) | 3.1 | ||||||||
Sucrose (mg/L) | 30 | 10 | 30 | 30 | 30 | 20 | 20 | 20 | 10 |
Vitamin Stock (ml/L) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||
Phytagel (g/L) | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
BAP (mg/L) | 2 | 6 | 1 | 0.75 | 3 | 0.00125 | |||
NAA (mg/L) | 0.1 | 0.2 | 0.2 | ||||||
IBA (mg/L) | 1 | ||||||||
AgNO3 (mg/L) | 5 | 5 | |||||||
GA3 (mg/L) | 0.01 | 0.01 | |||||||
CaCl2 (mg/L) | 435 | 435 | 435 | ||||||
KI (mg/L) | 0.75 | 0.75 | 0.75 | 0.375 | |||||
Adenine hemisulfate (mg/L) | 40 | ||||||||
PVP 40,000 (mg/L) | 500 |
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Farooq, N.; Nawaz, M.A.; Mukhtar, Z.; Ali, I.; Hundleby, P.; Ahmad, N. Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica. Plants 2019, 8, 558. https://doi.org/10.3390/plants8120558
Farooq N, Nawaz MA, Mukhtar Z, Ali I, Hundleby P, Ahmad N. Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica. Plants. 2019; 8(12):558. https://doi.org/10.3390/plants8120558
Chicago/Turabian StyleFarooq, Nisma, Muhammad Asif Nawaz, Zahid Mukhtar, Iftikhar Ali, Penny Hundleby, and Niaz Ahmad. 2019. "Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica" Plants 8, no. 12: 558. https://doi.org/10.3390/plants8120558