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Open AccessArticle

Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton

1
Plant Systems Engineering Research Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Korea
2
Department of Crop Science, Chungnam National University, 99 Daehak-ro, Daejeon 34143, Korea
3
Natural Medicine Research Center, KRIBB, 30 Yeongudanji-ro, Ochang-eup, Cheongju-si, Chungbuk 28116, Korea
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(6), 688; https://doi.org/10.3390/plants9060688
Received: 20 April 2020 / Revised: 25 May 2020 / Accepted: 25 May 2020 / Published: 28 May 2020
(This article belongs to the Special Issue Plant Tissue Culture)
Flavonoids, including maackiain (Maac) from Sophora flavescens Aiton roots, have many pharmacological properties, such as antitumor, antimicrobial, and antifungal activities. This research aimed to develop an in vitro plant and callus culture system for S. flavescens for the purpose of generating an alternative production system for enhancing Maac production, as Maac is usually present in very small amounts in S. flavescens’ roots. We arranged the optimal conditions of different tissues of S. flavescens and supplemented the medium with various plant growth regulators (PGRs). The highest induction and proliferation rates of callus was shown in combination treatments of all concentrations of thidiazuron (TDZ) and picloram. In addition, calli induced with leaf explants cultured on 2.0 mg/L picloram and 0.5 mg/L 6-benzyladenine (BA) in Murashige and Skoog (MS) medium had the highest accumulation of the active metabolite Maac. In vitro shoots were regenerated on medium containing combinations of TDZ and α-Naphthalene acetic acid (NAA). A reliable protocol for the mass production of secondary metabolites using a callus culture of S. flavescens was successfully established. View Full-Text
Keywords: callus induction; herbal resource; plant growth regulators; secondary metabolite; Sophora flavescens callus induction; herbal resource; plant growth regulators; secondary metabolite; Sophora flavescens
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Park, J.-S.; Seong, Z.-K.; Kim, M.-S.; Ha, J.-H.; Moon, K.-B.; Lee, H.-J.; Lee, H.-K.; Jeon, J.-H.; Park, S.U.; Kim, H.-S. Production of Flavonoids in Callus Cultures of Sophora flavescens Aiton. Plants 2020, 9, 688.

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