Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices
Blueberry Breeding and Genomics Laboratory, Horticultural Sciences Department, University of Florida, Gainesville, FL 32611, USA
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Plants 2020, 9(11), 1624; https://doi.org/10.3390/plants9111624
Received: 30 October 2020
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Revised: 19 November 2020
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Accepted: 19 November 2020
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Published: 23 November 2020
(This article belongs to the Special Issue Plant Tissue Culture)
The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants.
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Keywords:
blueberry; plant transformation; plant hormones; light quality; selection agents
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MDPI and ACS Style
Cappai, F.; Garcia, A.; Cullen, R.; Davis, M.; Munoz, P.R. Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices. Plants 2020, 9, 1624. https://doi.org/10.3390/plants9111624
AMA Style
Cappai F, Garcia A, Cullen R, Davis M, Munoz PR. Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices. Plants. 2020; 9(11):1624. https://doi.org/10.3390/plants9111624
Chicago/Turabian StyleCappai, Francesco, Alexandria Garcia, Ryan Cullen, Matthew Davis, and Patricio R. Munoz. 2020. "Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices" Plants 9, no. 11: 1624. https://doi.org/10.3390/plants9111624
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