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Open AccessArticle

Development of an In Vitro Method of Propagation for Artemisia tridentata subsp. tridentata to Support Genome Sequencing and Genotype-by-Environment Research

1
Department of Biological Sciences, Boise State University, Boise, ID 83725, USA
2
Department of Plant Sciences, Simplot, Boise, ID 83706, USA
*
Author to whom correspondence should be addressed.
Plants 2020, 9(12), 1717; https://doi.org/10.3390/plants9121717
Received: 27 October 2020 / Revised: 25 November 2020 / Accepted: 3 December 2020 / Published: 5 December 2020
(This article belongs to the Special Issue Plant Tissue Culture)
Basin big sagebrush (Artemisia tridentata subsp. tridentata) is a keystone species of the sagebrush steppe, a widespread ecosystem of western North America threatened by climate change. The study’s goal was to develop an in vitro method of propagation for this taxon to support genome sequencing and genotype-by-environment research on drought tolerance. Such research may ultimately facilitate the reintroduction of big sagebrush in degraded habitats. Seedlings were generated from two diploid mother plants (2n = 2x = 18) collected in environments with contrasting precipitation regimes. The effects of IBA and NAA on rooting of shoot tips were tested on 45 individuals and 15 shoot tips per individual. Growth regulator and individual-seedling effects on percent rooting and roots per shoot tip were evaluated using statistical and clustering analyses. Furthermore, rooted shoot tips were transferred into new media to ascertain their continued growth in vitro. The results suggest that A. tridentata is an outbred species, as shown by individuals’ effect on rooting and growth. IBA addition was the most effective method for promoting adventitious rooting, especially in top-performing individuals. These individuals also have high survival and growth rates upon transferring to new media, making them suitable candidates for generating biomass for genome sequencing and producing clones for genotype-by-environment research. View Full-Text
Keywords: drought; genotype; in vitro culture; plant growth regulators; sagebrush drought; genotype; in vitro culture; plant growth regulators; sagebrush
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MDPI and ACS Style

Barron, R.; Martinez, P.; Serpe, M.; Buerki, S. Development of an In Vitro Method of Propagation for Artemisia tridentata subsp. tridentata to Support Genome Sequencing and Genotype-by-Environment Research. Plants 2020, 9, 1717. https://doi.org/10.3390/plants9121717

AMA Style

Barron R, Martinez P, Serpe M, Buerki S. Development of an In Vitro Method of Propagation for Artemisia tridentata subsp. tridentata to Support Genome Sequencing and Genotype-by-Environment Research. Plants. 2020; 9(12):1717. https://doi.org/10.3390/plants9121717

Chicago/Turabian Style

Barron, Rachael; Martinez, Peggy; Serpe, Marcelo; Buerki, Sven. 2020. "Development of an In Vitro Method of Propagation for Artemisia tridentata subsp. tridentata to Support Genome Sequencing and Genotype-by-Environment Research" Plants 9, no. 12: 1717. https://doi.org/10.3390/plants9121717

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Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

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