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Open AccessArticle

Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes

1
Advanced Plant Technology Program, Clemson University, Clemson, SC 29634, USA
2
Department of Plant and Environmental Sciences, Clemson University, Clemson, SC 29634, USA
*
Author to whom correspondence should be addressed.
Plants 2020, 9(2), 248; https://doi.org/10.3390/plants9020248 (registering DOI)
Received: 3 January 2020 / Revised: 24 January 2020 / Accepted: 11 February 2020 / Published: 14 February 2020
(This article belongs to the Special Issue Plant Tissue Culture)
Sorghum has been considered a recalcitrant plant in vitro and suffers from a lack of regeneration protocols that function broadly and efficiently across a range of genotypes. This study was initiated to identify differential genotype-in vitro protocol responses across a range of bioenergy sorghum parental lines and the common grain sorghum genotype Tx430 in order to characterize response profiles for use in future genetic studies. Two different in vitro protocols, LG and WU, were used for comparisons. Distinct genotype-protocol responses were observed, and the WU protocol performed significantly better for plantlet regeneration. Most bioenergy genotypes performed as well, if not better than Tx430, with Rio and PI329311 as the top regenerating lines. Genotypes displayed protocol-dependent, differential phenolic exudation responses, as indicated by medium browning. During the callus induction phase, genotypes prone to medium browning exhibited a response on WU medium which was either equal or greater than on LG medium. Genotype- and protocol-dependent albino plantlet regeneration was also noted, with three of the bioenergy genotypes showing albino plantlet regeneration. Grassl, Rio and Pink Kafir were susceptible to albino plantlet regeneration, with the response strongly associated with the WU protocol. These bioenergy parental genotypes, and their differential responses under two in vitro protocols, provide tools to further explore and assess the role of genetic loci, candidate genes, and allelic variants in the regulation of in vitro responsiveness in sorghum. View Full-Text
Keywords: in vitro regeneration; medium browning; albino plantlet; genotype; bioenergy sorghum in vitro regeneration; medium browning; albino plantlet; genotype; bioenergy sorghum
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MDPI and ACS Style

Flinn, B.; Dale, S.; Disharoon, A.; Kresovich, S. Comparative Analysis of In Vitro Responses and Regeneration between Diverse Bioenergy Sorghum Genotypes. Plants 2020, 9, 248.

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