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Article

Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants

by 1,2,†, 3,†, 1,2, 1,2, 1,2, 3, 1,2,* and 1,2,*
1
Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, Guangzhou 510642, China
2
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou 510642, China
3
Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China
*
Authors to whom correspondence should be addressed.
These authors equally contributed to this work.
Plants 2020, 9(8), 997; https://doi.org/10.3390/plants9080997
Received: 17 June 2020 / Revised: 30 July 2020 / Accepted: 3 August 2020 / Published: 5 August 2020
(This article belongs to the Special Issue Plant Tissue Culture)
Magnolia lucida (Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype of M. lucida as the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth of M. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of α-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting in M. lucida plants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant. View Full-Text
Keywords: sterilization; shoot initiation; shoot proliferation; rooting; genetic fidelity assessment sterilization; shoot initiation; shoot proliferation; rooting; genetic fidelity assessment
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MDPI and ACS Style

Kang, L.; Zheng, K.; Xie, Y.; Deng, Y.; Yu, Y.; Zhu, M.; Xi, R.; Deng, X. Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants. Plants 2020, 9, 997. https://doi.org/10.3390/plants9080997

AMA Style

Kang L, Zheng K, Xie Y, Deng Y, Yu Y, Zhu M, Xi R, Deng X. Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants. Plants. 2020; 9(8):997. https://doi.org/10.3390/plants9080997

Chicago/Turabian Style

Kang, Lu, Keyuan Zheng, Yuqing Xie, Yanwen Deng, Yina Yu, Mulan Zhu, Ruchun Xi, and Xiaomei Deng. 2020. "Efficient Tissue Culture Protocol for Magnolia lucida (Magnoliaceae) and Confirmation of Genetic Stability of the Regenerated Plants" Plants 9, no. 8: 997. https://doi.org/10.3390/plants9080997

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