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Article

In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes

1
Center for Plant Water-Use and Nutrition Regulation, College of Life Sciences, Joint International Research Laboratory of Water and Nutrient in Cops, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
2
Sanming Academy of Agriculture Sciences, Sanming, Fujian 350002, China
3
Institute of Nuclear Agricultural Sciences, Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province, Zhejiang University, Hangzhou 310058, China
*
Authors to whom correspondence should be addressed.
Plants 2020, 9(3), 318; https://doi.org/10.3390/plants9030318
Received: 11 February 2020 / Revised: 28 February 2020 / Accepted: 1 March 2020 / Published: 3 March 2020
(This article belongs to the Special Issue Plant Tissue Culture)
The tissue culture regeneration system of Lupinus albus has always been considered as recalcitrant material due to its genotype-dependent response and low regeneration efficiency that hamper the use of genetic engineering. Establishment of repeatable plant regeneration protocol is a prerequisite tool for successful application of genetic engineering. This aim of this study was to develop standardized, efficient protocol for successful shoot induction from cotyledonary node of white lupin. In this study, 5 day old aseptically cultured seedlings were used to prepare three explants (half cotyledonary node, HCN; whole cotyledonary node, WCN; and traditional cotyledonary node, TCN), cultured on four concentrations of M519 medium (M519, ½ M519, 1/3 M519, and ¼ M519), containing four carbohydrate sources (sucrose, fructose, maltose, and glucose), and stimulated with various combinations of KT (kinetin), and NAA (naphthalene acetic acid) for direct shoot regeneration. High frequency of 80% shoot regeneration was obtained on ½ M519 medium (KT 4.0 mg L−1 + NAA 0.1 mg L−1) by using HCN as an explant. Interestingly, combinations of (KT 4.0 mg L−1 + NAA 0.1 mg L−1 + BAP 1.67 mg L−1), and (KT 2.0 mg L−1 + NAA 0.1 mg L−1) showed similar shoot regeneration frequency of 60%. Augmentation of 0.25 g L−1 activated charcoal (AC) not only reduced browning effect but also improved shoot elongation. Among the all carbohydrate sources, sucrose showed the highest regeneration frequency with HCN. Additionally, 80% rooting frequency was recorded on ½ M519 containing IAA 1.0 mg L−1 + KT 0.1 mg L−1 (indole acetic acid) after 28 days of culturing. The present study describes establishment of an efficient and successful protocol for direct plant regeneration of white lupin from different cotyledonary nodes. View Full-Text
Keywords: plant regeneration; shoot propagation; auxin; cytokinin; carbohydrate source; white lupin plant regeneration; shoot propagation; auxin; cytokinin; carbohydrate source; white lupin
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MDPI and ACS Style

Aslam, M.M.; Karanja, J.K.; Zhang, Q.; Lin, H.; Xia, T.; Akhtar, K.; Liu, J.; Miao, R.; Xu, F.; Xu, W. In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes. Plants 2020, 9, 318. https://doi.org/10.3390/plants9030318

AMA Style

Aslam MM, Karanja JK, Zhang Q, Lin H, Xia T, Akhtar K, Liu J, Miao R, Xu F, Xu W. In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes. Plants. 2020; 9(3):318. https://doi.org/10.3390/plants9030318

Chicago/Turabian Style

Aslam, Mehtab M., Joseph K. Karanja, Qian Zhang, Huifeng Lin, Tianyu Xia, Kashif Akhtar, Jianping Liu, Rui Miao, Feiyun Xu, and Weifeng Xu. 2020. "In Vitro Regeneration Potential of White Lupin (Lupinus albus) from Cotyledonary Nodes" Plants 9, no. 3: 318. https://doi.org/10.3390/plants9030318

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