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Open AccessArticle

The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera)

by 1,2,†, 1,3,†, 4, 1,2, 1, 1, 1,3, 1,3, 1,3, 1,2 and 1,2,*
Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan 430074, China
Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Wuhan 430074, China
College of Life Sciences, University of Chinese Academy of Sciences, 19A Yuquanlu, Beijing 100049, China
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, Wuhan 430070, China
Author to whom correspondence should be addressed.
These authors contributed equally: Xianbao Deng and Yaqian Xiong.
Plants 2020, 9(11), 1436;
Received: 25 August 2020 / Revised: 21 October 2020 / Accepted: 22 October 2020 / Published: 25 October 2020
(This article belongs to the Special Issue Plant Tissue Culture)
The lotus (Nelumbo nucifera) is one of the most popular aquatic plants in Asia, and has emerged as a novel model for studying flower and rhizome development, and primary and secondary metabolite accumulation. Here, we developed a highly efficient callus induction system for the lotus by optimizing a series of key factors that affect callus formation. The highest efficient callus production was induced on immature cotyledon and embryo explants grown on Murashige and Skoog (MS) basal medium containing an optimized combination of 3 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L 6-benzylaminopurine (6-BA). In addition, lotus callus induction was proven to be influenced by lotus genotypes, light conditions, the developmental stages of explants and the time of explant sampling. Collecting immature cotyledons from seeds of the genotype “Shilihe 1”, at 9 days post pollination, and to culture the explants in darkness, are proposed as the optimum conditions for lotus callus induction. Interestingly, highly efficient callus induction was also observed in explants of immature embryo derived aseptic seedlings; and a small amount of lotus benzylisoquinoline alkaloid (BIA) and obvious expression of BIA biosynthetic genes were detected in lotus callus. View Full-Text
Keywords: lotus; callus; induction; factors lotus; callus; induction; factors
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MDPI and ACS Style

Deng, X.; Xiong, Y.; Li, J.; Yang, D.; Liu, J.; Sun, H.; Song, H.; Wang, Y.; Ma, J.; Liu, Y.; Yang, M. The Establishment of an Efficient Callus Induction System for Lotus (Nelumbo nucifera). Plants 2020, 9, 1436.

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