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Microbial Enzyme Applications in Modern Food Fermentation

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Food Biotechnology".

Deadline for manuscript submissions: closed (10 August 2026) | Viewed by 1130

Editors

SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Interests: microbial enzymes; fermented food; food microbiology; quality improvement of fermented food

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Guest Editor
SKL of Marine Food Processing & Safety Control, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China
Interests: fermented food; food microbiology; metabolic engineering; microbial community structure; antioxidants; harmful metabolites during fermentation
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Special Issue Information

Dear Colleagues,

Microbial enzymes represent indispensable biocatalysts in modern food fermentation systems, driving advancements in product quality, safety, and sustainability. These enzymes enable precise modulation of biochemical pathways during fermentation, resulting in the targeted generation of flavor compounds that define sensory characteristics, while simultaneously transforming complex nutrients into bioavailable forms. Consequently, studies focused on microbial enzyme applications are crucial for advancing modern food fermentation technologies, optimizing production processes, and delivering superior quality fermented foods to consumers.

For this Special Issue of Foods titled “Microbial Enzyme Applications in Modern Food Fermentation”, we invite original research articles and comprehensive reviews addressing: (1) novel microbial enzyme sources or metabolic pathways enhancing fermentation efficiency; (2) microbial enzyme-mediated improvements in sensory, nutritional, or shelf-life attributes; (3) omics-based approaches (metagenomics, proteomics) to decode microbial community–enzyme interactions; and (4) immobilization and modification techniques improving microbial enzyme properties in continuous food fermentation systems.

Dr. Yiwei Dai
Prof. Dr. Sufang Zhang
Guest Editors

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Keywords

  • microbial enzyme
  • food fermentation
  • quality improvement
  • enzyme screening
  • enzyme immobilization
  • modification

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Published Papers (1 paper)

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Research

14 pages, 2356 KB  
Article
Enzymatic Properties of Chitosanase from Bacillus velezensis YB1534 and Antibacterial Activity of Its Oligosaccharide Products
by Yiwei Dai, Huiru Zhao, Jincui Wei, Yingxi Chen, Xinping Lin, Sufang Zhang and Chaofan Ji
Foods 2026, 15(3), 575; https://doi.org/10.3390/foods15030575 - 5 Feb 2026
Cited by 1 | Viewed by 730
Abstract
Chitosan oligosaccharides (COSs), obtained through the hydrolysis of chitosan, exhibit remarkable antibacterial properties. In pursuit of COSs with enhanced antibacterial activity, the enzymatic characteristics of the chitosanase from Bacillus velezensis YB1534 (BvChi) were investigated. The purified BvChi displayed optimal activity at pH 6.0 [...] Read more.
Chitosan oligosaccharides (COSs), obtained through the hydrolysis of chitosan, exhibit remarkable antibacterial properties. In pursuit of COSs with enhanced antibacterial activity, the enzymatic characteristics of the chitosanase from Bacillus velezensis YB1534 (BvChi) were investigated. The purified BvChi displayed optimal activity at pH 6.0 and 50 °C and showed the highest hydrolytic activity using colloidal chitosan as a substrate, with the presence of Mn2+. The COSs produced by enzymatic hydrolysis of BvChi exhibited a minimum degree of polymerization (DP) of 2, and their antimicrobial activities against certain pathogenic bacteria (Escherichia coli, Staphylococcus aureus, Salmonella typhi 50071, and Aeromonas hydrophila) were evaluated. Among them, the 20 min hydrolysate showed the strongest growth inhibition against all these pathogens, demonstrated by the inhibition zone diameters, and its MIC and MBC values toward A. hydrophila were 0.625 and 1.25 mg/mL, respectively. Thin-layer chromatography (TLC) analysis showed that the hydrolyzed products after 20 min contains more COSs with DP > 5. These findings highlighted the potential of BvChi as a biocatalyst for producing antimicrobial COSs, applicable in food preservation and biomedical fields. Full article
(This article belongs to the Special Issue Microbial Enzyme Applications in Modern Food Fermentation)
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