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Biosensors
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Molecularly Imprinted Polymers in Biosensors: Assembly, Characterization and Applications
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Molecularly Imprinted Polymers in Biosensors: Assembly, Characterization and Applications

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 25 December 2025 | Viewed by 263

Special Issue Editors

Prof. Dr. Maria Goreti Ferreira Sales

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Guest Editor
BioMark/CEMMPRE-ARISE, Faculty of Sciences and Technology, Coimbra University, 3030-790 Coimbra, Portugal
Interests: molecularly imprinted polymers; antibodies; electrochemical detection; optical detection; biosensors
Special Issues, Collections and Topics in MDPI journals
Dr. Jordi Riu

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Guest Editor
Department of Analytical Chemistry and Organic Chemistry, Faculty of Chemistry, Universitat Rovira i Virgili, 43007 Tarragona, Spain
Interests: array of sensors; data analysis; electrochemical detection; spectroscopy; biosensors; chemometrics
Special Issues, Collections and Topics in MDPI journals
Dr. Manuela F. Frasco

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Guest Editor
BioMark@UC, Faculty of Sciences and Technology, University of Coimbra, 3030-790 Coimbra, Portugal
Interests: nanomaterials; biopolymers; biomimetic imprinting; bioinspired photonics; optical sensors
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Molecularly imprinted polymers (MIPs) are synthetic biomimetic materials with highly selective recognition sites that are at the forefront of modern biosensor technology, transforming electrochemical/optical-photonic detection in terms of affordability, scalability and adaptability. They can be produced cheaply and quickly through a variety of polymerisation techniques, with all aspects of the production process being critical to creating a material suitable for use. MIPs also need to undergo appropriate characterisation procedures to validate their structural and functional properties. The applications of MIPs in biosensors are diverse and range from clinical diagnostics to environmental monitoring and food safety. Chemometrics is also considered a relevant approach concerning the assembly and application of MIP-based biosensors.

This Special Issue invites cutting-edge research and innovative reviews on the latest advances in MIP-based biosensors. We welcome contributions that explore new fabrication techniques, surface modifications, characterisation methods and new applications in the fields of healthcare, environmental monitoring, food safety and point-of-care diagnostics.

Prof. Dr. Maria Goreti Ferreira Sales
Dr. Jordi Riu
Dr. Manuela F. Frasco
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • molecularly imprinted polymers
  • biosensors
  • electropolymerisation
  • UV-initiated polymerisation
  • food analysis
  • environmental analysis
  • industrial follow-up
  • health diagnostics
  • biomimetic
  • paper-based analytical devices
  • lateral flow tests
  • microfluidics
  • electrochemical detection
  • optical detection

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Published Papers (2 papers)

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Research

16 pages, 2449 KiB  
Article
Enzyme-Free Monitoring of Glucose Using Molecularly Imprinted Polymers and Gold Nanoparticles
by Ana Rita Aires Cardoso, Pedro Miguel Cândido Barquinha and Maria Goreti Ferreira Sales
Biosensors 2025, 15(8), 537; https://doi.org/10.3390/bios15080537 - 15 Aug 2025
Abstract
This work describes a non-enzymatic electrochemical glucose biosensor combining for the first time molecularly imprinted polymers (MIPs) for glucose concentration and gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPEs), where both MIPs and AuNPs were assembled in situ. Electrochemical impedance spectroscopy (EIS) was [...] Read more.
This work describes a non-enzymatic electrochemical glucose biosensor combining for the first time molecularly imprinted polymers (MIPs) for glucose concentration and gold nanoparticles (AuNPs) on screen-printed carbon electrodes (SPEs), where both MIPs and AuNPs were assembled in situ. Electrochemical impedance spectroscopy (EIS) was used to evaluate the analytical performance of the sensor, which has a linear range between 1.0 µM and 1.0 mM when standard solutions are prepared in buffer. Direct measurement of glucose was performed by chronoamperometry, measuring the oxidation current generated during direct glucose oxidation. The selectivity was tested against ascorbic acid and the results confirmed a selective discrimination of the electrode for glucose. Overall, the work presented here represents a promising tool for tracking glucose levels in serum. The use of glucose MIP on the electrode surface allows the concentration of glucose, resulting in lower detection limits, and the use of AuNPs reduces the potential required for the oxidation of glucose, which increases selectivity. In addition, this possible combination of two analytical measurements following different theoretical concepts can contribute to the accuracy of the analytical measurements. This combination can also be extended to other biomolecules that can be electrochemically oxidised at lower potentials. Full article
(This article belongs to the Special Issue Molecularly Imprinted Polymers in Biosensors: Assembly, Characterization and Applications)
13 pages, 2094 KiB  
Article
Laser-Assisted Visible-Light Polymerization for Rapid Synthesis of Molecularly Imprinted Polymers
by Wissal Mrabet, Abdelhafid Karrat and Aziz Amine
Biosensors 2025, 15(8), 529; https://doi.org/10.3390/bios15080529 - 13 Aug 2025
Viewed by 40
Abstract
The demand for rapid, energy-efficient, and low-toxicity methods for synthesizing molecularly imprinted polymers (MIPs) is increasing, particularly for applications in environmental monitoring and green chemistry. In this context, the present work focuses on the development of a novel laser-assisted method for MIP synthesis, [...] Read more.
The demand for rapid, energy-efficient, and low-toxicity methods for synthesizing molecularly imprinted polymers (MIPs) is increasing, particularly for applications in environmental monitoring and green chemistry. In this context, the present work focuses on the development of a novel laser-assisted method for MIP synthesis, employing a visible laser (450 nm) and erythrosine B as a green photoinitiator. This visible-light approach enables fast and spatially controlled polymerization while avoiding the drawbacks of conventional methods (thermal heating, UV synthesis), such as the use of toxic initiators like AIBN and the need for UV shielding. MIPs were synthesized for bisphenol A and sulfamethoxazole, two emerging contaminants of significant environmental concern. The synthesis process was optimized for rapidity and scalability, and the resulting MIPs were integrated into a paper-based analytical device (MIP-PAD) for smartphone-assisted, on-site detection. The developed sensors exhibited excellent analytical performance, with recovery rates of 98.6% in tap water and 90.2% in river water and relative standard deviations (RSDs) below 1.88%. This study demonstrated a green, efficient, and highly controllable laser-assisted polymerization technique, offering a promising alternative to conventional MIP synthesis methods. Full article
(This article belongs to the Special Issue Molecularly Imprinted Polymers in Biosensors: Assembly, Characterization and Applications)
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