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Tropical Medicine and Infectious Disease, Volume 10, Issue 6

2025 June - 30 articles

Cover Story: While skin aspirates and biopsies are commonly used in clinical practice and supported by the main available guidelines for the diagnosis of cutaneous leishmaniasis (CL), non-invasive methods such as cutaneous swabs combined with molecular assays have recently shown promise. In a retrospective study conducted at an Italian tertiary center, including 29 CL cases, PCR on swabs resulted in 100% sensitivity (95% CI: 73.5–100) when compared to the reference diagnostic tests, namely Leishmania spp. culture, biopsy PCR, histology, or smear microscopy. Swab sampling with molecular testing offers a patient-friendly, effective diagnostic alternative that could become the first-line test for CL, with biopsy being reserved for more complex or suspicious cases. Further investigation is required to validate and standardize this method. View this paper
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Articles (30)

  • Article
  • Open Access
1,813 Views
17 Pages

Persistence of Untreated Bed Nets in the Retail Market in Tanzania: A Cross-Sectional Survey

  • Benjamin Kamala,
  • Dana Loll,
  • Ruth Msolla,
  • David Dadi,
  • Peter Gitanya,
  • Charles Mwalimu,
  • Frank Chacky,
  • Stella Kajange,
  • Mwinyi Khamis and
  • Stephen Poyer
  • + 2 authors

The private sector in Tanzania has played an essential role in improving coverage and access to mosquito nets. This follow-up study assessed the overall market share for untreated and insecticide-treated nets (ITNs) and misleading or counterfeit ITN...

  • Article
  • Open Access
771 Views
14 Pages

Similar Microsatellite Allelic Distribution Between Anopheles darlingi Population Collected by Human Landing Catch or Mosquito Magnet Traps in French Guiana

  • Laetitia Ferraro,
  • Sébastien Briolant,
  • Mathieu Nacher,
  • Samuel Vezenegho,
  • Antoine Adde,
  • Christophe Nguyen,
  • Pascal Gaborit,
  • Jean Issaly,
  • Romuald Carinci and
  • Hervé Bogreau
  • + 3 authors

Anopheles darlingi is a major malaria vector in South America. Understanding its population dynamics is critical for designing effective vector control strategies. While various Anopheles collection methods exist, they may sample distinct populations...

  • Article
  • Open Access
3 Citations
3,033 Views
17 Pages

Epidemiological Trends and Age–Period–Cohort Effects on Dengue Incidence Across High-Risk Regions from 1992 to 2021

  • Yu Cao,
  • Hanwu Chen,
  • Hao Wu,
  • Bin Wu,
  • Lu Wang,
  • Xin Liu,
  • Yuyue Yang,
  • Hui Tan and
  • Wei Gao

Dengue, an acute infectious disease caused by the dengue virus, remains a major public health problem in the 21st century. This study investigated the global dengue burden, identified high-risk regions, evaluated the long-term incidence trends, and c...

  • Article
  • Open Access
1,298 Views
16 Pages

Effectiveness of Pyrethroid-Piperonyl Butoxide Nets Versus Standard Pyrethroid-Only Nets in Preventing Malaria in Children Under 10 Years Living in Kisantu Health Zone, Democratic Republic of the Congo

  • Gillon Ilombe,
  • Joris Losimba Likwela,
  • Philippe Lukanu,
  • Aimée Lulebo,
  • Nicole Muela,
  • Joachim Mariën,
  • Kennedy Makola Mbanzulu,
  • Baby Mabanzila,
  • Junior Rika Matangila and
  • Seth R. Irish
  • + 6 authors

Democratic Republic of the Congo (DRC) is among the countries that have a high malaria incidence. In an effort to combat this public health challenge, innovative tools and strategies are being developed and evaluated. Among the new generation of nets...

  • Article
  • Open Access
3 Citations
1,675 Views
21 Pages

Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes in Aedes aegypti

  • Yamili Contreras-Perera,
  • Lucy Mackenzie-Impoinvil,
  • Dieunel Derilus,
  • Audrey Lenhart,
  • Iram P. Rodriguez-Sanchez,
  • Pablo Manrique-Saide and
  • Adriana E. Flores

The rapid global expansion of Aedes aegypti-borne diseases such as dengue, chikungunya, and Zika has positioned this mosquito as a key target for vector control programs. These programs rely heavily on insecticide use, leading to the widespread emerg...

  • Article
  • Open Access
1,972 Views
13 Pages

Epidemiological Analysis of the COVID-19 Clusters in the Early Stages of the Epidemic in Shanghai, China: Pandemic-to-Epidemic Response Shift

  • Dechuan Kong,
  • Qiwen Fang,
  • Jian Chen,
  • Linjie Hu,
  • Yihan Lu,
  • Yaxu Zheng,
  • Yiyi Zhu,
  • Bihong Jin,
  • Wenjia Xiao and
  • Huanyu Wu
  • + 9 authors

As COVID-19 transitions from pandemic to endemic, our prevention and control policies have shifted from broad, strict community interventions to focusing on the prevention of cluster outbreaks. Currently, information on the characteristics of cluster...

  • Article
  • Open Access
1 Citations
1,114 Views
11 Pages

Serological Vulnerability and Active Infection Detection Among Recently Arrived Migrants in Spain: Results from a Targeted Screening Program

  • Guillermo Lens-Perol,
  • Olalla Vázquez-Cancela,
  • Magdalena Santana-Armas,
  • Angeles Bouzas-Rodriguez,
  • Victoria Tuñez-Bastida,
  • Adrián Domínguez-Lago,
  • Hugo Pérez-Freixo,
  • Cristina Peiteado-Romay,
  • Juan Manuel Vázquez-Lago and
  • Cristina Fernández-Pérez

Background: Newly arrived migrants are at increased risk for vaccine-preventable and communicable diseases due to low immunization coverage, poor access to healthcare, and challenging migration trajectories. This study describes the implementation an...

  • Article
  • Open Access
1 Citations
2,862 Views
18 Pages

Risk of Incidence and Lethality by Etiology of Severe Acute Respiratory Syndrome in Hospitalized Children Under 1 Year of Age in Brazil in 2024: A Cross-Sectional Study

  • Tamires de Nazaré Soares,
  • Natasha Cristina Oliveira Andrade,
  • Suziane do Socorro dos Santos,
  • Marcela Raíssa Asevedo Dergan,
  • Karina Faine Freitas Takeda,
  • Jully Greyce Freitas de Paula Ramalho,
  • Luany Rafaele da Conceição Cruz,
  • Perla Katheleen Valente Corrêa,
  • Marli de Oliveira Almeida and
  • Luana Nepomuceno Gondim Costa Lima
  • + 4 authors

Severe Acute Respiratory Syndrome (SARS) represents a significant cause of morbidity and mortality in children under one year of age, a particularly vulnerable population due to immunological and respiratory immaturity. The diverse etiology includes...

  • Article
  • Open Access
2 Citations
1,625 Views
32 Pages

There is a significant portion of the South African population with unknown HIV status, which slows down epidemic control despite the progress made in HIV testing. Machine learning (ML) has been effective in identifying individuals at higher risk of...

  • Article
  • Open Access
1 Citations
1,458 Views
13 Pages

Immunological and Virological Responses in Patients with Monoinfection and Coinfection with Hepatitis B and C Viruses in the Brazilian Amazon

  • Joseane R. Silva,
  • Regiane M. A. Sampaio,
  • Patrícia F. Nunes,
  • Vanessa S. Guimarães,
  • Camila Carla da Silva Costa,
  • Evelen da Cruz Coelho,
  • Micheline Vale de Souza,
  • Luana Wanessa Cruz Almeida,
  • Hellen T. Fuzii and
  • Luisa C. Martins
  • + 1 author

Infections with the Hepatitis B (HBV) and Hepatitis C (HCV) viruses share some transmission routes, which is why coinfection with these viruses becomes common, especially in endemic areas. This study evaluated the immunological response profile, vira...

  • Case Report
  • Open Access
1 Citations
1,167 Views
14 Pages

Emergence of Multidrug-Resistant Campylobacter jejuni in a Common Variable Immunodeficiency Patient: Evolution of Resistance Under the Selective Antibiotic Pressure

  • Tajana Juzbašić,
  • Nataša Andrijašević,
  • Ivana Ferenčak,
  • Dragan Jurić,
  • Silvija Šoprek,
  • Vlatka Poje Janeš,
  • Ljiljana Žmak,
  • Arjana Tambić Andrašević and
  • Ana Gverić Grginić

Campylobacter jejuni is a leading cause of bacterial gastroenteritis worldwide which usually presents as mild, and self-limiting disease in immunocompetent individuals. However, in immunocompromised patients, such as those with common variable immuno...

  • Article
  • Open Access
2 Citations
3,555 Views
15 Pages

Bacterial Pathogens and Antibiotic Resistance in Bloodstream Infections in Tunisia: A 13-Year Trend Analysis

  • Lamia Kanzari,
  • Sana Ferjani,
  • Khaoula Meftah,
  • Mariem Zribi,
  • Sonda Mezghani,
  • Asma Ferjani,
  • Yosra Chebbi,
  • Manel Hamdoun,
  • Hajer Rhim and
  • Ilhem Boutiba-Ben Boubaker
  • + 28 authors

The antimicrobial resistance (AMR) surveillance network has been monitoring bloodstream bacterial pathogens and their resistance since 1999 in Tunisia. We report the long-term trends in the distribution of bloodstream bacterial pathogens and their re...

  • Case Report
  • Open Access
1 Citations
8,198 Views
13 Pages

Cutaneous larva migrans (CLM) is attributed to zoonotic infection with animal hookworm larvae penetrating the human skin, usually the feet and legs. There is, however, a broad range of differential diagnoses, with the implicated hookworm species usua...

  • Article
  • Open Access
2 Citations
2,223 Views
12 Pages

From Knowledge to Practice: The Effect of Multimodal Strategies on Hand Hygiene Improvement in Tunisia

  • Maissa Ben Jmaa,
  • Mariem Ben Hmida,
  • Houda Ben Ayed,
  • Hanen Maamri,
  • Maroua Trigui,
  • Nimer Ortuño-Gutiérrez,
  • Aelita Sargsyan,
  • Mondher Kassis,
  • Rony Zachariah and
  • Sourour Yaich

Hand hygiene reduces healthcare-associated infections. The World Health Organization (WHO) recommends the multimodal hand hygiene strategy to improve hand hygiene. To compare hand hygiene knowledge and compliance of healthcare practitioners (HCPs) be...

  • Article
  • Open Access
3,253 Views
16 Pages

Chagas Disease in a Non-Endemic Setting: Clinical Profile, Treatment Outcomes, and Predictors of Cure in a 15-Year Cohort Study

  • Carlos Bea-Serrano,
  • Ana Isabel de Gracia-León,
  • Jara Llenas-García,
  • Sara Vela-Bernal,
  • Andreu Belmonte-Domingo,
  • Carolina Pinto-Pla,
  • Ana Ferrer-Ribera,
  • María José Galindo,
  • María Jesús Alcaraz and
  • María Rosa Oltra Sempere

This retrospective cohort study aimed to assess clinical and epidemiological characteristics, treatment outcomes, and predictors of serological cure in patients with chronic Chagas disease in a non-endemic setting. All individuals aged ≥16 years w...

  • Article
  • Open Access
3,038 Views
13 Pages

Development and Validation of a New Set of Primers for Identification of Circulating Lineages and Palivizumab/Nirsevimab Resistance in HRSV Isolates from Cabo Verde

  • María Paula Reyes-Zuluaga,
  • José Antonio Pérez-Pérez,
  • Wilson Correia,
  • Isabel Inês M. de Pina Araújo and
  • Emma Carmelo

In Cabo Verde, Acute Respiratory Infection caused by various pathogens was the most reported condition in children under 5 years old between 2014–2020, and the fourth leading cause of mortality in this age group, with Human Respiratory Syncytia...

  • Article
  • Open Access
1 Citations
2,127 Views
14 Pages

Epidemiological Characteristics and Trends of Zoonotic Diseases in China from 2015 to 2022

  • Yunfei Zhang,
  • Mengjie Geng,
  • Yue Shi,
  • Baijun Jin,
  • Qian Xiong,
  • Sheng Zhou,
  • Jiangmei Liu,
  • Biao Kan,
  • Maigeng Zhou and
  • Canjun Zheng
  • + 1 author

This study aimed to analyze the epidemiological characteristics and incidence trends of zoonotic diseases in China from 2015 to 2022, providing evidence for zoonotic disease prevention and control strategies. Individual case data for nationally repor...

  • Article
  • Open Access
1,449 Views
12 Pages

Molecular Assays on Cutaneous Swabs as an Effective, Non-Invasive Diagnostic Technique for Cutaneous Leishmaniasis: Results from a Retrospective Study Conducted in Italy

  • Anna Barbiero,
  • Andrea Aiello,
  • Nunziata Ciccone,
  • Simona Pollini,
  • Francesca Malentacchi,
  • Maria Grazia Colao,
  • Gian Maria Rossolini,
  • Costanza Fiorelli,
  • Daniela Massi and
  • Lorenzo Zammarchi
  • + 5 authors

Background: The case confirmation of CL relies on the direct demonstration of the parasite in clinical specimens from skin tissues. Despite most research efforts focusing on biopsy samples as the preferred diagnostic specimen for the detection of Lei...

  • Article
  • Open Access
2 Citations
2,607 Views
27 Pages

Syndemic Synergy of HPV, HIV, and HSV-2 for Oncogenic HPV Replication in Female Sex Workers

  • Jonathan Muwonga Tukisadila,
  • Ralph-Sydney Mboumba Bouassa,
  • Serge Tonen-Wolyec,
  • Hugues Loemba,
  • Jeremie Muwonga and
  • Laurent Belec

Background: Female sex workers (FSWs) in sub-Saharan Africa bear a disproportionate burden of sexually transmitted infections, including HIV, high-risk HPV (HR-HPV), and herpes simplex virus type 2 (HSV-2). This study evaluated possible association b...

  • Article
  • Open Access
2,376 Views
8 Pages

Baylisascariasis is a debilitating and potentially lethal zoonotic disease caused by a nematode parasite that has a worldwide distribution. Baylisascaris spp. are carried by a variety of mammalian definitive hosts, and their larvae can infect a large...

  • Systematic Review
  • Open Access
2 Citations
4,746 Views
16 Pages

Current Overview of Environmental Disinfection and Decolonization of C. auris: A Systematic Review from 2020 to 2025

  • María Guadalupe Frías-De-León,
  • Paola Betancourt-Cisneros,
  • Erick Martínez-Herrera,
  • Paola Berenice Zarate-Segura,
  • Carlos Alberto Castro-Fuentes and
  • Eduardo García-Salazar

Candida auris possesses distinctive features that facilitate its persistence and transmission in healthcare settings, causing outbreaks of infection that are difficult to treat. So, emphasis has been placed on implementing measures for controlling, e...

  • Article
  • Open Access
2 Citations
1,210 Views
10 Pages

Background and Objectives: Abnormal blood and biochemical indicators could increase the risk of infectious diseases. However, the association between blood together with biochemical indicators and latent tuberculosis infection (LTBI) has not been wel...

  • Article
  • Open Access
2,188 Views
15 Pages

Screening and Diagnosis Access for Neglected and Tropical Parasitic Diseases in Italy: A National Survey

  • Agnese Comelli,
  • Ester Oliva,
  • Francesco Bernieri,
  • Lorenzo Zammarchi,
  • Libera Clemente,
  • Luciana Petrullo,
  • Guido Calleri,
  • Fabrizio Bruschi and
  • Annibale Raglio

Background: The availability of laboratory tests to screen and diagnose migrants and travellers for neglected and tropical parasitic diseases significantly impacts individual and public health. Italian scientific societies for parasitology, tropical...

  • Review
  • Open Access
4 Citations
7,571 Views
16 Pages

Advances in Diagnostic Techniques for Influenza Virus Infection: A Comprehensive Review

  • Qi Qian,
  • Guohao Fan,
  • Wei Yang,
  • Chenguang Shen,
  • Yang Yang,
  • Yingxia Liu and
  • Weiwei Xiao

Influenza poses a significant global health burden due to its high transmissibility, antigenic variability, and substantial morbidity. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has further complicated influenza dynamic...

  • Review
  • Open Access
4 Citations
2,499 Views
18 Pages

The Association Between Environmental Factors and Scrub Typhus: A Review

  • Shu Yang,
  • Shu Yang,
  • Yuxiang Xie,
  • Wenjing Duan,
  • Yiting Cui,
  • Ai Peng,
  • Yisheng Zhou,
  • Yibing Fan,
  • Hui Li and
  • Peng Huang

Scrub typhus is an acute febrile vector-borne infectious disease caused by Orientia tsutsugamushi (O. tsutsugamushi) and transmitted through the bite of infected chigger mite larvae. Transmission involves complex ecological interactions among vectors...

  • Review
  • Open Access
2,237 Views
20 Pages

One Health Landscape in Tennessee: Current Status, Challenges, and Priorities

  • Walid Q. Alali,
  • Jane Yackley,
  • Katie Garman,
  • Debra L. Miller,
  • Ashley Morgan,
  • Wesley Crabtree,
  • Sonia Mongold,
  • Dan Grove,
  • Emily Leonard and
  • Mary-Margaret A. Fill

Tennessee’s ecological diversity, spanning forests, farmland, and urban areas, provides an ideal foundation for applying the One Health approach, which integrates human, animal, and environmental health. This review examines Tennessee’s c...

  • Article
  • Open Access
2 Citations
1,884 Views
19 Pages

Studies have indicated that there was a disease interaction of pandemic influenza with respiratory tuberculosis (TB) in Malta, which could explain the heightened mortality recorded in young adults. We revisit the 1918/19 influenza and TB syndemic pot...

  • Case Report
  • Open Access
1 Citations
1,736 Views
7 Pages

Unmasking Bacillus Calmette–Guérin Immune Reconstitution Inflammatory Syndrome in a Perinatal HIV Transmission—A Case Report

  • Daniel Ivanov,
  • Dimitar Strashimirov,
  • Rusina Grozdeva,
  • Evgeni Penchev,
  • Elena Georgieva and
  • Nina Yancheva

Bacillus Calmette–Guérin (BCG)-related immune reconstitution inflammatory syndrome (IRIS) is a recognised complication following antiretroviral therapy (ART) initiation in HIV-infected infants. We report the case of a 19-month-old child...

  • Article
  • Open Access
3,350 Views
11 Pages

Physical and Chemical Characteristics of Aedes aegypti Larval Habitats in Nouakchott, Mauritania

  • Mohamed Haidy Massa,
  • Mohamed Aly Ould Lemrabott,
  • Osman Abdillahi Guedi,
  • Sébastien Briolant and
  • Ali Ould Mohamed Salem Boukhary

Aedes aegypti, the main urban vector of dengue fever, represents a growing public health problem in Nouakchott, the capital of Mauritania. Identifying the factors influencing the distribution and productivity of its breeding sites is essential for th...

  • Article
  • Open Access
2,331 Views
18 Pages

Burden in Colombia of COVID-19 in Adults and the Associated Clinical Characteristics: A Retrospective Database Analysis

  • Jair Arciniegas,
  • Juan Manuel Reyes,
  • Jhon Bolaños-López,
  • Julia Regazzini Spinardi,
  • Jingyan Yang,
  • Farzaneh Maleki,
  • Farley Johanna Gonzalez,
  • Carlos Jose Bello,
  • Ana Catalina Herrera-Díaz and
  • Carlos Fernando Mendoza
  • + 6 authors

Studies on the burden of COVID-19 cases in Colombia have focused on specific populations and short timeframes. A retrospective observational study was conducted on adult patients aged 18 diagnosed with COVID-19 who received inpatient and/or outpatien...

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Trop. Med. Infect. Dis. - ISSN 2414-6366