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Biomolecules, Volume 15, Issue 10
October 2025 - 135 articles
Cover Story: Reactive oxygen species (ROS) are key effectors of innate immunity. During the respiratory burst (RB), human neutrophils reduce oxygen to superoxide, forming hydrogen peroxide (H2O2) and altering membrane potential. We tested whether extracellular potassium affects H2O2 production, as potassium levels can rise during necrosis or trauma. H2O2 release was measured over 2 h under varying potassium concentrations, with no clear effects. Substituting potassium or chloride with sodium or methanesulfonate likewise caused no change. Thus, unlike electrically excitable cells such as neurons and muscles, the strongly depolarizing RB of human neutrophils appears independent of monovalent ion concentrations, though hyperosmolar solutions do affect it. View this paper
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Biomolecules - ISSN 2218-273X