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Volume 16
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J. Funct. Biomater., Volume 16, Issue 4 (April 2025) – 31 articles

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27 pages, 4323 KiB  
Review
A Review of Past Research and Some Future Perspectives Regarding Titanium Alloys in Biomedical Applications
by Alex-Barna Kacsó and Ildiko Peter
J. Funct. Biomater. 2025, 16(4), 144; https://doi.org/10.3390/jfb16040144 - 18 Apr 2025
Abstract
This review paper provides a comprehensive synthesis of the current advancements in investigations of different titanium-based alloys, including pure titanium, commercially available Ti6Al4V, and modified alloys, such as Ti-Nb-Zr-Fe alloys, for biomedical applications. Several researchers have explored the effects of alloying elements and [...] Read more.
This review paper provides a comprehensive synthesis of the current advancements in investigations of different titanium-based alloys, including pure titanium, commercially available Ti6Al4V, and modified alloys, such as Ti-Nb-Zr-Fe alloys, for biomedical applications. Several researchers have explored the effects of alloying elements and processing techniques on enhancing the mechanical, chemical, and biological properties of these materials. Ti-Nb-Zr-Fe alloys are of particular interest due to their potential to address critical requirements in medical applications, including reduced Young’s modulus, superior corrosion resistance, biocompatibility, and mechanical strength. Despite substantial progress, the detailed mechanisms for optimizing these properties remain underexplored in the current literature. The main objective of the present review paper is to emphasize the importance of ongoing investigations aimed at overcoming challenges such as biocompatibility concerns, fatigue resistance, and wear under biological conditions. By critically analyzing existing data, this study highlights gaps in knowledge and identifies opportunities for advancing research on these alloys. Specifically, this review paper highlights the need for targeted studies to reduce the Young’s modulus and improve other critical characteristics of Ti-Nb-Zr-Fe alloys to better meet the demands of orthopedic implants, dental prosthetics, and cardiovascular devices. Even if the current scientific literature is ample on this topic, we consider that through this review we can positively contribute to the collective effort in this field trying to fill some gaps, including some updates on the topic, time frames, advantages, and limitations, and pave the way for further advancements that could revolutionize biomedical implant technology. The review encompasses studies performed over the last 5 decades, specifically from 1975 to 2025, to ensure the inclusion of the most relevant and up-to-date research. This approach aims to highlight the significant progress made while situating the findings within the broader context of ongoing investigations. Full article
(This article belongs to the Special Issue Metals and Alloys for Biomedical Application)
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17 pages, 6300 KiB  
Article
Finite Element Simulation of Biomechanical Effects on Periodontal Ligaments During Maxillary Arch Expansion with Thermoformed Aligners
by Gustavo A. Rojas, Jose Isidro García-Melo and Juan S. Aristizábal
J. Funct. Biomater. 2025, 16(4), 143; https://doi.org/10.3390/jfb16040143 - 17 Apr 2025
Viewed by 8
Abstract
Purpose: This paper investigates the biomechanical effect of thermoformed aligners equipped with complementary biomechanical attachments (CBAs) on periodontal ligaments (PDLs) during the expansion process of the maxillary arch. The analysis was conducted using advanced simulations based on the finite element method (FEM). Methods: [...] Read more.
Purpose: This paper investigates the biomechanical effect of thermoformed aligners equipped with complementary biomechanical attachments (CBAs) on periodontal ligaments (PDLs) during the expansion process of the maxillary arch. The analysis was conducted using advanced simulations based on the finite element method (FEM). Methods: High-resolution 3D CAD models were created for four tooth types: canine, first premolar, second premolar, and first molar. Additional 3D models were developed for aligners, CBAs, and PDLs. These were integrated into a comprehensive FEM model to simulate clinical rehabilitation scenarios. Validation was achieved through comparative analysis with empirical medical data. Results: The FEM simulations revealed the following: for canine, the displacement was 0.134 mm with a maximum stress of 4.822 KPa in the amelocemental junction. For the first premolar, the displacement was 0.132 mm at a maximum stress of 3.273 KPa in the amelocemental junction. The second premolar had a displacement of 0.129 mm and a stress of 1.358 KPa at 1 mm from the amelocemental junction; and first molar had a displacement of 0.124 mm and a maximum stress of 2.440 KPa. Conclusions: The inclusion of CBAs significantly reduced tooth tipping during maxillary arch expansion. Among the models tested, the vestibular CBA demonstrated superior performance, delivering optimal tooth movement when combined with thermoformed aligners. Significance: FEM techniques provide a robust and cost-effective alternative to in vivo experimentation, offering precise and reliable insights into the biomechanical efficacy of CBAs in thermoformed aligners. This approach minimizes experimental variability and accelerates the evaluation of innovative orthodontic configurations. Full article
(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry)
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14 pages, 5488 KiB  
Article
Enhanced Osteoconductivity of Zirconia Implants with One-Step Femtosecond Laser Treatment Through Morphological and Chemical Modifications
by Yuqi Li, Yanzhe Fu, Nan Li, Guanqi Liu, Jiebo Li, Jiao Wen and Jianmin Han
J. Funct. Biomater. 2025, 16(4), 142; https://doi.org/10.3390/jfb16040142 - 15 Apr 2025
Viewed by 97
Abstract
Improving surface bioactivity is crucial to acquiring zirconia implants with ideal osteoconductivity. In this work, we enhanced the surface properties of zirconia implants, specifically roughness, hydrophilicity, and osteoconductivity, using a “one-step” femtosecond laser (FSL) treatment in air, deionized water, and sodium hydroxide solution. [...] Read more.
Improving surface bioactivity is crucial to acquiring zirconia implants with ideal osteoconductivity. In this work, we enhanced the surface properties of zirconia implants, specifically roughness, hydrophilicity, and osteoconductivity, using a “one-step” femtosecond laser (FSL) treatment in air, deionized water, and sodium hydroxide solution. Zirconia specimens were treated in these media, and their surface morphology, chemical composition, and osteoconductivity were evaluated through various assays. The results showed that FSL treatment successfully created micro/nanoporous structures and increased roughness across all specimens. The liquid media treatment facilitated the grafting of hydroxyl (-OH) groups, significantly improving hydrophilicity. The L-NaOH group exhibited a higher hydroxyl content (28%) compared to the L-Air group (10%), reducing the contact angle significantly. Enhanced osteoblast differentiation and mineralization, along with improved gene expression, were observed in the L-Water and L-NaOH groups. In conclusion, the one-step FSL treatment developed a dual-function bioactive zirconia surface, offering an effective method for the biomedical functionalization of zirconia implants. Full article
(This article belongs to the Section Dental Biomaterials)
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16 pages, 2252 KiB  
Article
Impact of Vitamin D3 Functionalization on the Osteogenic Capacity of Bioinspired 3D Scaffolds Based on Ce-Doped Bioactive Glass and Spongia Agaricina
by Ana-Maria Seciu-Grama, Sorana Elena Lazăr, Simona Petrescu, Oana Cătălina Mocioiu, Oana Crăciunescu and Irina Atkinson
J. Funct. Biomater. 2025, 16(4), 141; https://doi.org/10.3390/jfb16040141 - 14 Apr 2025
Viewed by 96
Abstract
Reconstruction of extensive bone defects due to age, trauma, or post-illness conditions remains challenging. Biomimetic scaffolds with osteogenic capabilities have been proposed as an alternative to the classical autograft and allograft implants. Three-dimensional scaffolds were obtained based on Ce-doped mesoporous bioactive glass (MBG) [...] Read more.
Reconstruction of extensive bone defects due to age, trauma, or post-illness conditions remains challenging. Biomimetic scaffolds with osteogenic capabilities have been proposed as an alternative to the classical autograft and allograft implants. Three-dimensional scaffolds were obtained based on Ce-doped mesoporous bioactive glass (MBG) and Spongia agaricina (SA) as sacrificial templates functionalized with vitamin D3. The study aimed to investigate the effect of vitamin D3 functionalization on the optimal variant of a 3D scaffold doped with 3 mol% ceria, selected in our previous work based on its biological and physicochemical properties. Scanning electron microscopy (SEM) images of the non-functionalized/functionalized scaffolds revealed a porous structure with interconnected pores ranging from 100 to 350 μm. Fourier transform infrared spectroscopy (FTIR) and SEM analysis confirmed the surface functionalization. Cytotoxicity evaluation showed that all investigated scaffolds do not exhibit cytotoxicity and genotoxicity toward the Saos-2 osteosarcoma cell line. Moreover, the study demonstrated that functionalization with vitamin D3 enhanced osteogenic activity in dental pulp stem cells (DPSCs) by increasing calcium deposition and osteocalcin secretion, as determined by Alizarin red stain and a colorimetric ELISA kit, as a result of its synergistic action with cerium ions. The results showed that the Ce-doped MBG scaffold functionalized with vitamin D3 had the potential for applications in bone regeneration. Full article
(This article belongs to the Special Issue Functional Biomaterial for Bone Regeneration)
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12 pages, 3312 KiB  
Article
Surface Micromorphology of Experimental Composites Doped with Bioactive Glass After Different Storage Times
by Leonardo Svellenti, Moritz Tanner, Andrea Gubler, Matej Par, Thomas Attin, Phoebe Burrer and Tobias T. Tauböck
J. Funct. Biomater. 2025, 16(4), 140; https://doi.org/10.3390/jfb16040140 - 14 Apr 2025
Viewed by 72
Abstract
Objective: To evaluate the surface micromorphology of bioactive glass-modified resin composite materials after storage in simulated body fluid for different periods of time and ultrasonic cleaning. Materials and methods: A resin composite material (Heliomolar Flow, Ivoclar Vivadent) was modified by incorporating 10 or [...] Read more.
Objective: To evaluate the surface micromorphology of bioactive glass-modified resin composite materials after storage in simulated body fluid for different periods of time and ultrasonic cleaning. Materials and methods: A resin composite material (Heliomolar Flow, Ivoclar Vivadent) was modified by incorporating 10 or 20 wt% of bioactive glass 45S5. The unmodified conventional composite (0 wt% bioactive glass) served as the control. Surface morphology of light-cured composite specimens was examined by profilometry both before and after storage in simulated body fluid (SBF; pH = 7.4, t = 37 °C) for 0, 3, 7, or 30 days, and surface roughness (Ra) was recorded. After storage, ultrasonic cleaning (UC) of the specimens was performed for 10 min in an ultrasonic bath filled with deionized water, and the profilometric measurements were subsequently repeated. In addition, the surfaces of specimens were examined by scanning electron microscopy (SEM). Results: Directly after specimen preparation, the Ra values of the composites modified with bioactive glass were similar to those of the conventional composite (0 wt% bioactive glass). A longer immersion in SBF and higher added concentrations of bioactive glass led to an increase in surface roughness. SEM examination revealed that precipitates were formed on the surfaces of specimens containing bioactive glass after exposure to SBF for at least 7 days. The density of these precipitates increased with exposure time and added bioactive glass content. After subsequent ultrasonic cleaning, a significant Ra reduction was observed for specimens containing 10 and 20 wt% bioactive glass and stored for 30 days (p < 0.001). For the resin composite material doped with 20 wt% bioactive glass particles, UC revealed a significant Ra reduction at all time points. Conclusion: The increase in the surface roughness of bioactive glass-modified composites after storage in SBF might be partly attributed to precipitate formation on their surfaces. After ultrasonic cleaning, surface roughness was still increased, indicating poorer surface quality compared to conventional composite. Full article
(This article belongs to the Special Issue New Trends in Biomaterials and Implants for Dentistry (2nd Edition))
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15 pages, 4954 KiB  
Article
Evaluation of the Characteristics of Digital Light Processing 3D-Printed Magnesium Calcium Phosphate for Bone Regeneration
by Peng Zhang, Meiling Zhang, Yoo-Na Jung, Seong-Won Choi, Yong-Seok Lee, Geelsu Hwang and Kwi-Dug Yun
J. Funct. Biomater. 2025, 16(4), 139; https://doi.org/10.3390/jfb16040139 - 14 Apr 2025
Viewed by 128
Abstract
Recent advancements in three-dimensional (3D) printing technology, particularly digital light processing (DLP) 3D printing, have enabled the customization of bone substitutes with specific shapes that match bone defect sizes and geometries. Magnesium calcium phosphate (MCP) has gained considerable attention due to its strong [...] Read more.
Recent advancements in three-dimensional (3D) printing technology, particularly digital light processing (DLP) 3D printing, have enabled the customization of bone substitutes with specific shapes that match bone defect sizes and geometries. Magnesium calcium phosphate (MCP) has gained considerable attention due to its strong mechanical properties, degradability, and ability to promote bone regeneration. In this study, we prepared MCP samples with five different molar ratios via DLP 3D printing. We analyzed the physicochemical properties of these five groups, including phase compositions and microstructures, which were examined using X-ray diffraction and scanning electron microscopy, respectively. Additionally, we assessed the effects of MCP on material density and shrinkage. Biaxial flexural strength and degradation rate were evaluated; biological properties were examined through WST-8 analysis and alkaline phosphatase activity assays. Among the tested samples, MCP1/1 exhibited the highest strength. A higher proportion of magnesium phosphate in MCP corresponded to an increased degradation rate. Cell response observations in the WST-8 assay indicated that cell proliferation was better in the MCP1/1 group than in the other groups on days 4 and 7 of culturing. Alkaline phosphatase activity assays demonstrated that MCP1/1 exhibited higher activity than calcium phosphate. Our findings suggest that MCP1/1 can be used effectively in bone-tissue-engineering applications. Full article
(This article belongs to the Special Issue State of the Art: Three-Dimensional Printing Materials and Regenerative Medicine)
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10 pages, 677 KiB  
Article
An Assessment of the Micro-Tensile Bond Strength of Composites for Indirect Restoration to Enamel and Dentin
by Viktoria Petrova, Janet Kirilova and Sevda Yantcheva
J. Funct. Biomater. 2025, 16(4), 138; https://doi.org/10.3390/jfb16040138 - 12 Apr 2025
Viewed by 72
Abstract
This study aimed to evaluate the micro-tensile bond strength (µTBS) of two types of composites for indirect restoration, luted to enamel and dentin with self-adhesive cement. Moreover, it aimed to evaluate the impact of thermocycling on bond strength. Sixteen flat enamel and dentin [...] Read more.
This study aimed to evaluate the micro-tensile bond strength (µTBS) of two types of composites for indirect restoration, luted to enamel and dentin with self-adhesive cement. Moreover, it aimed to evaluate the impact of thermocycling on bond strength. Sixteen flat enamel and dentin surfaces of human molars were cemented to equal flat specimens of the laboratory composite Signum ceramis and the CAD/CAM block Cerasmart. Half of the specimens of the group underwent thermocycling. After that, the samples were cut into 80 beams for µTBS analysis. The data were analyzed using Levene’s test and the independent sample t-test. The micro-tensile bond strength tests revealed that thermocycling significantly reduced the adhesive bond. Dentin bonds better to conventional laboratory composites. Enamel bonds are better than composite blocks for milling. Full article
(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry)
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11 pages, 1432 KiB  
Article
Thermal Dynamics of Laser-Irradiated Trilayer Bonded-Zirconia Structures
by Mitchell Tharp, Jaccare Jauregui-Ulloa, Grace Mendonça De Souza and Susana Salazar Marocho
J. Funct. Biomater. 2025, 16(4), 137; https://doi.org/10.3390/jfb16040137 - 11 Apr 2025
Viewed by 72
Abstract
This study aims to assess the thermal dynamics of supporting structures during laser-assisted debonding of bonded yttrium-stabilized zirconia (YSZ) ceramic. We tested the hypothesis that the heat transfer to dentin analog material and composite resin resembles that of dentin. Thirty sintered YSZ (ZirCAD, [...] Read more.
This study aims to assess the thermal dynamics of supporting structures during laser-assisted debonding of bonded yttrium-stabilized zirconia (YSZ) ceramic. We tested the hypothesis that the heat transfer to dentin analog material and composite resin resembles that of dentin. Thirty sintered YSZ (ZirCAD, Ivoclar, Schann, Liechtenstein) slabs (4 mm diameter, 1 mm thickness) were air particle abraded, followed by two coats of Monobond Plus (Ivoclar). The slabs were bonded to exposed occlusal dentin, NEMA G10 dentin analog, or composite resin cylinders using Multilink Automix (Ivoclar) dual-cured cement. The bonded YSZ specimens (n = 10/group) subjected to irradiation with an Er,Cr:YSGG laser (Waterlase MD, Biolase, Foothill Ranch, CA, USA) at 7.5 W, 25 Hz, with 50% water and air for 15 s. Heat transfer during laser irradiation was monitored with an infrared camera (Optris PI 640, Optris GmbH, Berlin, Germany) at 0.1-s intervals. Data were analyzed using one-way ANOVA, which showed no significant differences in mean temperature between zirconia and cement layers across the substrates (composite resin, G10, dentin) (p = 0.0794). These results suggest flexibility in substrate choice for future thermal dynamics studies under laser irradiation. Full article
(This article belongs to the Special Issue Property, Evaluation and Development of Dentin Materials)
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22 pages, 5152 KiB  
Review
Therapeutic Potential of Nano-Sustained-Release Factors for Bone Scaffolds
by Haoran Jiang, Meng Zhang, Yang Qu, Bohan Xing, Bojiang Wang, Yanqun Liu and Peixun Zhang
J. Funct. Biomater. 2025, 16(4), 136; https://doi.org/10.3390/jfb16040136 - 9 Apr 2025
Viewed by 155
Abstract
Research on nano-sustained-release factors for bone tissue scaffolds has significantly promoted the precision and efficiency of bone-defect repair by integrating biomaterials science, nanotechnology, and regenerative medicine. Current research focuses on developing multifunctional scaffold materials and intelligent controlled-release systems to optimize the spatiotemporal release [...] Read more.
Research on nano-sustained-release factors for bone tissue scaffolds has significantly promoted the precision and efficiency of bone-defect repair by integrating biomaterials science, nanotechnology, and regenerative medicine. Current research focuses on developing multifunctional scaffold materials and intelligent controlled-release systems to optimize the spatiotemporal release characteristics of growth factors, drugs, and genes. Nano slow-release bone scaffolds integrate nano slow-release factors, which are loaded with growth factors, drugs, genes, etc., with bone scaffolds, which can significantly improve the efficiency of bone repair. In addition, these drug-loading systems have also been extended to the fields of anti-infection and anti-tumor. However, the problem of heterotopic ossification caused by high doses has led to a shift in research towards a low-dose multi-factor synergistic strategy. Multiple Phase II clinical trials are currently ongoing, evaluating the efficacy and safety of nano-hydroxyapatite scaffolds. Despite significant progress, this field still faces a series of challenges: the immunity risks of the long-term retention of nanomaterials, the precise matching of multi-factor release kinetics, and the limitations of the large-scale production of personalized scaffolds. Future development directions in this area include the development of responsive sustained-release systems, biomimetic sequential release design, the more precise regeneration of injury sites through a combination of gene-editing technology and self-assembled nanomaterials, and precise drug loading and sustained release through microfluidic and bioprinting technologies to reduce the manufacturing cost of bone scaffolds. The progress of these bone scaffolds has gradually changed bone repair from morphology-matched filling regeneration to functional recovery, making the clinical transformation of bone scaffolds safer and more universal. Full article
(This article belongs to the Special Issue Mesoporous Nanomaterials for Bone Tissue Engineering)
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27 pages, 9840 KiB  
Review
Antibiotic Action, Drug Delivery, Biodegradability, and Wound Regeneration Characteristics of Surgical Sutures and Cutting-Edge Surgical Suture Manufacturing Technologies
by Hye-Ree Han
J. Funct. Biomater. 2025, 16(4), 135; https://doi.org/10.3390/jfb16040135 - 8 Apr 2025
Viewed by 257
Abstract
(1) Background: With the emergence of various super bacteria, interest in antibacterial properties, drug delivery, and wound regeneration is increasing in the field of surgical materials. There are many studies on surgical sutures, but not many recent ones that have studied structurally subdivided [...] Read more.
(1) Background: With the emergence of various super bacteria, interest in antibacterial properties, drug delivery, and wound regeneration is increasing in the field of surgical materials. There are many studies on surgical sutures, but not many recent ones that have studied structurally subdivided functions. Accordingly, various studies on surgical sutures were classified based on the main functions that are considered important, and studies were conducted by categorizing the latest production technology into 3D printing and electrospinning. (2) Methods: Data from the literature (n = 1077) were collected from databases such as PubMed, Harvard.edu, MDPI, Google Scholar, Web of Science, ACS, Nature, and IOP Publishing. The selected 103 papers were divided into two main groups: cutting-edge characteristics of surgical sutures and the latest technologies for manufacturing surgical sutures. (3) Results: Cutting-edge characteristics of surgical sutures were divided into four major categories: antibacterial, drug delivery, biodegradability, and wound regeneration, and examined in depth. In addition, the final technologies for manufacturing surgical sutures were divided into electrospinning and 3D printing. (4) Conclusions: The results of this study can contribute to the development of multifunctional surgical sutures that promote wound regeneration through antibacterial properties, drug elution, and biodegradability. Full article
(This article belongs to the Topic New Challenges in the Cosmetics Industry)
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17 pages, 16835 KiB  
Article
Evaluation of Biomechanical Effects of Mandible Arch Types in All-on-4 and All-on-5 Dental Implant Design: A 3D Finite Element Analysis
by Sema Nur Sevinç Gül, Fahri Murat and Abdullah Tahir Şensoy
J. Funct. Biomater. 2025, 16(4), 134; https://doi.org/10.3390/jfb16040134 (registering DOI) - 7 Apr 2025
Viewed by 113
Abstract
This study evaluates the biomechanical effects of different implant configurations in various mandibular arch types using finite element analysis (FEA). Stress distribution and deformation patterns were analyzed under different loading conditions in square, U-shaped, and V-shaped arches. The results indicate that increasing the [...] Read more.
This study evaluates the biomechanical effects of different implant configurations in various mandibular arch types using finite element analysis (FEA). Stress distribution and deformation patterns were analyzed under different loading conditions in square, U-shaped, and V-shaped arches. The results indicate that increasing the number of implants generally reduces cortical bone stress, particularly in U and V arches, while implant-level stress tends to increase. Under molar loading, cortical bone stress in the square arch decreased by 16.9% (from 90.61 MPa to 75.27 MPa) with the All-on-5 system, while implant stress in the V arch dropped by 46.26% (from 142.35 MPa to 76.5 MPa). Additionally, the cantilever effect in All-on-4 configurations resulted in higher stress on the prosthesis and implants, particularly in V arches. While the All-on-5 system provided better load distribution, the study highlights the importance of optimizing implant positioning based on mandibular anatomy. Despite limitations such as the use of static forces and standardized arch types, these findings offer valuable insights into the biomechanical performance of full-arch implant rehabilitations, supporting future clinical applications and research. Full article
(This article belongs to the Section Dental Biomaterials)
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23 pages, 4048 KiB  
Systematic Review
Insights into Sinus-Lift Bone Grafting Materials: What’s Changed?
by Anida-Maria Băbțan, Claudia N. Feurdean, Anca Ionel, Willi A. Uriciuc, Radu Chifor, Chambon Antoine Bernard Jaques, Bianca A. Boșca and Aranka Ilea
J. Funct. Biomater. 2025, 16(4), 133; https://doi.org/10.3390/jfb16040133 - 7 Apr 2025
Viewed by 324
Abstract
Background: Sinus-lift (SL) is a pre-prosthetic procedure with the objective of increasing bone height to achieve implant insertion primary stability in implant-supported prostheses. The biomechanical properties of SL augmentation materials are influenced by their origin, manufacture, bioactive substances addition, receiver, and surgical procedure. [...] Read more.
Background: Sinus-lift (SL) is a pre-prosthetic procedure with the objective of increasing bone height to achieve implant insertion primary stability in implant-supported prostheses. The biomechanical properties of SL augmentation materials are influenced by their origin, manufacture, bioactive substances addition, receiver, and surgical procedure. This systematic review provides insights into state-of-the-art SL biomaterials, focusing on autologous bone grafting as the gold standard. Methods: The study followed the PRISMA flow diagram, searching WoS (Web of Science), Embase, Cochrane, and PubMed databases using the search terms «sinus lift» OR «sinus augmentation» OR «bone graft» OR «bovine» OR «porcine» OR «autologous» OR «allogenic» OR «xenogeneic» OR «alloplastic» OR «hydroxyapatite» OR «β-tricalcium phosphate (β-TCP)» OR «equine» OR «PRF». Results: The highest bone gain was provided by Bioglass at 42%. Articles written between 2014 and 2024 in English or French, containing human studies and with full text available, were included. Participants were required to be in good general health, without acute, chronic, or congenital diseases, or substance abuse (drugs, alcohol, or nicotine). SL surgery was performed using the lateral approach, with no Schneiderian membrane perforation or postoperative complications. The network meta-analysis was conducted using the R statistical computing environment. To assess the inconsistency between direct and indirect evidence, we used a net heat plot. To evaluate heterogeneity across studies, we used the chi-squared-based Q-test and I2 statistic. A significance level of 0.05 was applied throughout all analyses. Results: Allogeneic bovine bone and hydrox yapatite demonstrated the lowest resorption rates. Significant differences were found for residual graft and connective tissue between allogenous bovine bone (ABB) + AlB vs. β-TCP + PRF (p = 0.028); ABB + AlB vs. β-TCP (p = 0.034); ABB + AlB vs. BCP (p = 0.037). Meta-analysis showed that the overall heterogeneity was 51.8% (6.9–75%; p = 0.019), with significant heterogeneity within designs (p = 0.007) and no significant heterogeneity between designs (p = 0.39). AB had a better bone regeneration ratio compared to many of the other interventions, but only two passed the threshold of significance: A1B and B-TCP + AB. Conclusions: A grafting material’s superiority is determined by its new bone formation ratio, connective tissue integration, residual graft content, and bone resorptionratio. Although autologous bone grafting has exhibited superior bone regeneration compared to other biomaterials, it was not favored due to its unpredictable connective tissue concentration and bone resorption ratio. Additionally, autologous bone exhibited the fastest metabolic turnover among all grafting materials. Full article
(This article belongs to the Special Issue Bone Regeneration and Repair Materials, 2nd Edition)
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16 pages, 2188 KiB  
Article
Non-Thermal Atmospheric Plasma Enhances Biological Effects of Fluoride on Oral Biofilms
by Anushri Warang, Isha Deol, Sarah Fakher, Linfeng Wu, Liang Hong, Shaoping Zhang, Qingsong Yu and Hongmin Sun
J. Funct. Biomater. 2025, 16(4), 132; https://doi.org/10.3390/jfb16040132 - 5 Apr 2025
Viewed by 95
Abstract
The objective of this study was an assessment of the anti-biofilm properties of fluoride non-thermal atmospheric plasma (FNTAP) generated using argon and hydrocarbon fluoride gas 1,1,1,2-tetrafluoroethane (TFE). These properties were evaluated by measuring the destruction and recovery of in vitro dual-species biofilms of [...] Read more.
The objective of this study was an assessment of the anti-biofilm properties of fluoride non-thermal atmospheric plasma (FNTAP) generated using argon and hydrocarbon fluoride gas 1,1,1,2-tetrafluoroethane (TFE). These properties were evaluated by measuring the destruction and recovery of in vitro dual-species biofilms of Streptococcus mutans and Streptococcus sanguinis exposed to FNTAP at 5 or 10 standard cubic centimeters per minute (sccm) or argon non-thermal atmospheric plasma (ArNTAP) for 1 or 2 min, using resazurin-based reagent viability assays, colony forming units (CFU), culture media pH and live/dead staining. Both ArNTAP and FNTAP resulted in significant immediate reductions in bacterial load as compared to the control. Although ArNTAP did not significantly reduce biofilm regrowth, FNTAP treatment showed a bacterial load reduction of more than 5 log units of biofilm regrowth. FNTAP treatments significantly reduced the acidification of the culture medium after recovery incubation, indicating reduced living bacteria, with a pH of 6.92 ± 0.02 and 6.90 ± 0.03, respectively, for the 5 sccm and 10 sccm FNTAP treatments, as compared to a pH of 5.83 ± 0.26 for the ArNTAP treatment, and a significantly acidic pH of 4.76 ± 0.04 for the no-treatment groups. Our results suggest that FNTAP has exceptional anti-biofilm effects, and future directions of our research include the assessment of potential applications of FNTAP in clinical settings. Full article
(This article belongs to the Special Issue Surface Analyses, Physicochemical and Mechanical Properties of Dental Biomaterials (2nd Edition))
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14 pages, 2215 KiB  
Article
Physico-Chemical Properties and Push-Out Bond Strength to Root Dentine of Calcium Silicate-Based Sealers
by Ivana Milanovic, Vesna Miletic, Bojan Dzeletovic, Djordje Antonijevic, Tatjana Savic Stankovic, Danilo Pavlovic, Ana Despotovic and Violeta Petrovic
J. Funct. Biomater. 2025, 16(4), 131; https://doi.org/10.3390/jfb16040131 - 3 Apr 2025
Viewed by 112
Abstract
The calcium silicate-based sealers currently available on the market have different compositions and formulations, which is why their physical and chemical properties may vary. (1) The aim of the study was to measure the physico-chemical properties of calcium silicate-based sealers and their push-out [...] Read more.
The calcium silicate-based sealers currently available on the market have different compositions and formulations, which is why their physical and chemical properties may vary. (1) The aim of the study was to measure the physico-chemical properties of calcium silicate-based sealers and their push-out bond strength to root dentine, comparing two push-out testing protocols. (2) Standardized specimens of EndoSequence BC, BioRoot RCS, MTA Fillapex, and AH Plus (control) were subjected to pH measurements over 28 days. Radiopacity was measured using a CCD sensor, and flexural strength was assessed using a three-point bending setup. Push-out bond strength was measured in coronal, middle, and apical sections of 40 single-root teeth (conventional method), and cylindrical cavities were prepared for all sealers on the same root dentine disks in 11 third molars (disk method). (3) EndoSequence BC exhibited a higher pH than MTA Fillapex and the highest radiopacity (p < 0.05). The highest flexural and push-out bond strengths were found for AH Plus. The push-out bond strength of EndoSequence BC and BioRoot RCS was higher than MTA Fillapex (p < 0.05). The conventional and disk methods exhibited similar push-out bond strength results, but the data were more homogeneously distributed in the disk method. (4) All calcium silicate-based sealers exhibited a higher pH than AH Plus. MTA Fillapex did not meet the ISO standard. Calcium silicate-based sealers showed weaker performance in terms of physical properties compared to AH Plus. Full article
(This article belongs to the Special Issue Advances in Biomaterials for Reconstructive Dentistry)
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27 pages, 1365 KiB  
Review
Commercial Biomaterial-Based Products for Tendon Surgical Augmentation: A Scoping Review on Currently Available Medical Devices
by Marta Pluchino, Leonardo Vivarelli, Gianluca Giavaresi, Dante Dallari and Marco Govoni
J. Funct. Biomater. 2025, 16(4), 130; https://doi.org/10.3390/jfb16040130 - 3 Apr 2025
Viewed by 192
Abstract
Tendon defect is one of the common clinical diseases related to the growing population mean age and the number of athletes. Due to an increasing demand for tendon repair surgical interventions, several tendon augmentation products, capable of guaranteeing the necessary biological and visco-elasticity [...] Read more.
Tendon defect is one of the common clinical diseases related to the growing population mean age and the number of athletes. Due to an increasing demand for tendon repair surgical interventions, several tendon augmentation products, capable of guaranteeing the necessary biological and visco-elasticity properties and mechanical support, have been developed. In this regard, commercially available products may be grouped into three main categories: (i) natural, (ii) synthetic, and (iii) hybrid biomaterial-based products. Firstly, to better define the research area of this work, common search engines were employed to acquire information from reports or website portfolios of important competitors in the global tendon repair market. Secondly, public registries and bibliographic databases were also employed to analyse data from registered clinical trials and published clinical studies performed to evaluate the safety and efficacy of each product. Ten new products have been launched on the market in the last fifteen years: advantages, disadvantages, and future perspectives regarding their use for tendon augmentation treatment are discussed. Although hybrid biomaterial-based products may be considered as more oriented to the new frontiers of tendon augmentation technology, future improvements, especially focused on both mechanical properties and biocompatibility, are needed. However, scientific innovations must navigate convoluted clinical regulatory paths, which, due to high costs for investors, long development timelines, and funding shortages, hinder the translation of many scientific discoveries into routine clinical practice. Full article
(This article belongs to the Special Issue The 15th Anniversary of JFB—Innovative Biomaterials for Tissue Engineering: Regeneration of Soft and Hard Tissues)
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21 pages, 2270 KiB  
Review
Role of Endophytic Fungi in the Biosynthesis of Metal Nanoparticles and Their Potential as Nanomedicines
by Hanadi Sawalha, Simon E. Moulton, Andreas Winkel, Meike Stiesch and Bita Zaferanloo
J. Funct. Biomater. 2025, 16(4), 129; https://doi.org/10.3390/jfb16040129 - 3 Apr 2025
Viewed by 234
Abstract
Metal nanoparticles (MNPs) produced through biosynthesis approaches have shown favourable physical, chemical, and antimicrobial characteristics. The significance of biological agents in the synthesis of MNPs has been acknowledged as a promising alternative to conventional approaches such as physical and chemical methods, which are [...] Read more.
Metal nanoparticles (MNPs) produced through biosynthesis approaches have shown favourable physical, chemical, and antimicrobial characteristics. The significance of biological agents in the synthesis of MNPs has been acknowledged as a promising alternative to conventional approaches such as physical and chemical methods, which are confronted with certain challenges. To meet these challenges, the use of endophytic fungi as nano-factories for the synthesis of MNPs has become increasingly popular worldwide in recent times. This review provides an overview of the synthesis of MNPs using endophytic fungi, the mechanisms involved, and their important biomedical applications. A special focus on different biomedical applications of MNPs mediated endophytic fungi involved their antibacterial, antifungal, antiviral, and anticancer applications and their potential as drug delivery agents. Furthermore, this review highlights the significance of the use of endophytic fungi for the green synthesis of MNPs and discusses the benefits, challenges, and prospects in this field. Full article
(This article belongs to the Collection Feature Papers in Antibacterial Biomaterials)
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23 pages, 5564 KiB  
Article
The Evaluation of Restored Proximal Contact Areas with Four Direct Adherent Biomaterials: An In Vitro Study
by Elena-Cristina Marcov, Mihai Burlibașa, Narcis Marcov, Florentina Căminișteanu, Andreea Angela Ștețiu, Mircea Popescu, Radu-Cătălin Costea, Raluca Mariana Costea, Liliana Burlibașa, Andi Ciprian Drăguș, Maria Antonia Ștețiu and Dana Cristina Bodnar
J. Funct. Biomater. 2025, 16(4), 128; https://doi.org/10.3390/jfb16040128 - 3 Apr 2025
Viewed by 138
Abstract
The aim of this study was to compare the interproximal contact tightness of lateral teeth after restoring adjacent proximal walls with four types of direct adherent biomaterials. Distal and mesial boxes were prepared on 160 artificial right first and second upper molars. Each [...] Read more.
The aim of this study was to compare the interproximal contact tightness of lateral teeth after restoring adjacent proximal walls with four types of direct adherent biomaterials. Distal and mesial boxes were prepared on 160 artificial right first and second upper molars. Each set of 40 pairs of boxes was restored using one bulk biomaterial: Equia Forte Fil HT (GC), Cention® Forte (IVOCLAR VIVADENT), Admira Fusion x-tra (VOCO), or 3MTMFiltekTM One Bulk Fill. The mean difference in the passing-through force varied from sound to restored surfaces immediately after application, as well as at 7 and 14 days after: Equia Forte Fil HT—4.07 ± 0.01, 4.08 ± 0.01, and 4.11 ± 0.01; Cention® Forte—3.30 ± 0.01, 3.50 ± 0.01, and 3.56 ± 0.01; Admira Fusion x-tra—4.10 ± 0.01, 4.13 ± 0.01, and 4.13 ± 0.01; 3MTMFiltekTM One Bulk Fill—4.08 ± 0.01, 4.09 ± 0.01, and 4.07 ± 0.01 (p < 0.05). The passing-through force of the restored contact areas showed significantly higher values when compared to those for the sound surfaces, and among them, all biomaterials presented similar values, except for Cention® Forte. The potential clinical relevance of this study relates to better knowing the most appropriate restorative material for large proximal caries on adjacent surfaces from the outset of the treatment protocol. Full article
(This article belongs to the Special Issue Biomaterials in Conservative Dentistry and Prosthodontics (2nd Edition))
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20 pages, 1258 KiB  
Review
Application of Hydroxyapatite Composites in Bone Tissue Engineering: A Review
by Weijie Liu, Nalini Cheong, Zhuling He and Tonghan Zhang
J. Funct. Biomater. 2025, 16(4), 127; https://doi.org/10.3390/jfb16040127 - 2 Apr 2025
Viewed by 206
Abstract
The treatment of bone defects is complicated by clinical conditions, such as trauma, tumor resection, and infection, which result in defects and impair the bone’s regenerative capacity. Hydroxyapatite (HAp), the primary inorganic component of bone, possesses good biocompatibility and osteoconductivity. However, it has [...] Read more.
The treatment of bone defects is complicated by clinical conditions, such as trauma, tumor resection, and infection, which result in defects and impair the bone’s regenerative capacity. Hydroxyapatite (HAp), the primary inorganic component of bone, possesses good biocompatibility and osteoconductivity. However, it has poor mechanical properties, a slow degradation rate, and limited functionality, necessitating combination with other materials to broaden its application scope. This paper summarizes the importance and properties of HAp composites and provides a categorized review of current research on HAp composites in bone tissue engineering. These composite scaffolds not only offer excellent mechanical support for cell growth and tissue regeneration but also facilitate new bone formation and vascularization. Additionally, the challenges faced by HAp composites, such as material property optimization and improvement of preparation techniques, are discussed. The paper also summarizes the applications of HAp composites in bone defect repair, dental implants, spinal fusion, and other fields. Full article
(This article belongs to the Special Issue Hydroxyapatite Composites for Biomedical Application)
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31 pages, 25096 KiB  
Article
Study of the Structure and Mechanical Properties of Ti-38Zr-11Nb Alloy
by Konstantin V. Sergienko, Sergei V. Konushkin, Yaroslava A. Morozova, Mikhail A. Kaplan, Artem D. Gorbenko, Boris A. Rumyantsev, Mikhail E. Prutskov, Evgeny E. Baranov, Elena O. Nasakina, Tatiana M. Sevostyanova, Sofia A. Mikhlik, Andrey P. Chizhikov, Lyudmila A. Shatova, Aleksandr V. Simakin, Ilya V. Baimler, Maria A. Sudarchikova, Mikhail L. Kheifetz, Alexey G. Kolmakov and Mikhail A. Sevostyanov
J. Funct. Biomater. 2025, 16(4), 126; https://doi.org/10.3390/jfb16040126 - 2 Apr 2025
Viewed by 121
Abstract
Hip joint implants are among the most prevalent types of medical implants utilized for the replacement of damaged joints. The utilization of modern implant materials, such as cobalt–chromium alloys, stainless steel, titanium, and other titanium alloys, is accompanied by challenges, including the toxicity [...] Read more.
Hip joint implants are among the most prevalent types of medical implants utilized for the replacement of damaged joints. The utilization of modern implant materials, such as cobalt–chromium alloys, stainless steel, titanium, and other titanium alloys, is accompanied by challenges, including the toxicity of certain elements (e.g., aluminum, vanadium, nickel) and excessive Young’s modulus, which adversely impact biomechanical compatibility. A mismatch between the stiffness of the implant material and the bone tissue, known as stress shielding, can lead to adverse outcomes such as bone resorption and implant loosening. Recent studies have shifted the focus to β-titanium alloys due to their exceptional biocompatibility, corrosion resistance, and low Young’s modulus, which is close to the Young’s modulus of bone tissue (10–30 GPa). In this study, the microstructure, mechanical properties, and phase stability of the Ti-38Zr-11Nb alloy were investigated. Energy dispersion spectrometry was employed to confirm the homogeneous distribution of Ti, Zr, and Nb in the alloy. A subsequent microstructural analysis revealed the presence of elongated β-grains subsequent to rolling and quenching. Furthermore, grinding contributed to the process of recrystallization and the formation of subgrains. X-ray diffraction analysis confirmed the presence of a stable β-phase under any heat treatment conditions, which can be explained by the use of Nb as a β-stabilizer and Zr as a neutral element with a weak β-stabilizing effect in the presence of other β-stabilizers. Furthermore, the modulus of elasticity, as determined by tensile testing, exhibited a decline from 85 GPa to 81 GPa after annealing. Mechanical tests demonstrated a substantial enhancement in tensile strength (from 529 MPa to 628 MPa) concurrent with a 32% reduction in elongation to fracture of the samples. These alterations are attributed to microstructural transformations, including the formation of subgrains and the rearrangement of dislocations. This study’s findings suggest that the Ti-38Zr-11Nb alloy has potential as a material of choice due to its lower Young’s modulus compared to traditional materials and its stable β-phase, which enhances the implant’s durability and reduces the risk of brittle phases forming over time. This study demonstrates that the corrosion resistance of titanium grade 2 and Ti-38Zr-11Nb is comparable. The material in question exhibited no evidence of cytotoxic activity in the context of mammalian cells. Full article
(This article belongs to the Section Bone Biomaterials)
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16 pages, 4998 KiB  
Article
In Vitro Cytotoxicity Evaluation of Nanosized Hydroxyapatite and Fluorapatite on Cell Lines and Their Relevance to the Alveolar Augmentation Process
by Wojciech Zakrzewski, Maria Szymonowicz, Anna Nikodem, Agnieszka Rusak, Zbigniew Rybak, Katarzyna Szyszka, Dorota Diakowska, Benita Wiatrak, Rafal J. Wiglusz and Maciej Dobrzyński
J. Funct. Biomater. 2025, 16(4), 125; https://doi.org/10.3390/jfb16040125 - 2 Apr 2025
Viewed by 220
Abstract
Background/Objectives: Materials with an apatite structure were investigated in vitro in dental bone augmentation procedures. This scientific study aimed to compare nanosized hydroxyapatite (nHAp) and fluorapatite (nFAp) materials in the form of tablets in in vitro studies, including cytotoxicity assessment and fluoride release. [...] Read more.
Background/Objectives: Materials with an apatite structure were investigated in vitro in dental bone augmentation procedures. This scientific study aimed to compare nanosized hydroxyapatite (nHAp) and fluorapatite (nFAp) materials in the form of tablets in in vitro studies, including cytotoxicity assessment and fluoride release. Methods: The nHAp and nFAp nanosized materials were obtained using the microwave hydrothermal method. Subsequently, the tablets were prepared from these nanosized powders as further studied materials. Cytotoxicity tests were conducted on Balb/3T3 fibroblast cells and L929 cells. Fluoride ion release was tested at 3, 24, 48, 72, and 168 h periods. Results: Both materials presented viability levels above 70%, indicating a lack of cytotoxic potential. The amount of fluoride (F−) ions released and accumulated from nFAp was greatly higher than from nHAp. The release of F− ions in both samples was the highest in the first 3 h of exposition. The accumulation of F− ions reached the highest values in the deionized water. The most significant differences in the released or cumulated fluoride ions were observed between deionized water and lower 4.5 pH AS (artificial saliva) samples. Conclusions: Both nanosized hydroxyapatite and fluorapatite materials are biocompatible, and their in vitro examination showed promising results for their future in vivo application. Full article
(This article belongs to the Special Issue Functional Nanoparticles/Nanocomposites for Biomedical Applications)
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18 pages, 3167 KiB  
Article
A Tea Polyphenol-Infused Sprayable Thermosensitive Liposomal Hydrogel for Enhanced Anti-Inflammatory and Antibacterial Psoriasis Treatment
by Wei Shen, Qilian Ye, Hongbo Zhang, Shenghong Xie, Shiqi Xie, Cailian Chen, Jinying Liu, Zhengwei Huang, Hai-Bin Luo and Ling Guo
J. Funct. Biomater. 2025, 16(4), 124; https://doi.org/10.3390/jfb16040124 - 1 Apr 2025
Viewed by 106
Abstract
Psoriasis is a chronic and recurrent inflammatory disease driven not only by intrinsic factors such as immune system dysregulation but also by external factors, including bacterial infections. In contrast to the control of a single pathogenic pathway, combination therapies addressing both the immune [...] Read more.
Psoriasis is a chronic and recurrent inflammatory disease driven not only by intrinsic factors such as immune system dysregulation but also by external factors, including bacterial infections. In contrast to the control of a single pathogenic pathway, combination therapies addressing both the immune and infectious components of psoriasis pathogenesis may offer a more effective strategy for controlling its progression. In this study, we developed a sprayable hydrogel incorporating tea polyphenol-loaded lauric acid liposomes (TP@LA-Lipo gel) to investigate its anti-inflammatory and antibacterial role in psoriasis. Our results demonstrated that TP@LA-Lipo modulated macrophage activity, reduced the expression of iNOS and TNF-α, and remodeled the immune microenvironment. Meanwhile, TP@LA-Lipo effectively eliminated Staphylococcus aureus and Escherichia coli through membrane disruption, mitigating the provoked inflammatory response. More importantly, TP@LA-Lipo gel, when sprayed onto the psoriasis lesions, provided sustained drug release over three days, enabling deeper penetration through the thickened stratum corneum to reach the inflamed layers beneath. Furthermore, in an imiquimod-induced psoriasis mouse model, TP@LA-Lipo gel effectively restored the damaged skin, alleviated histopathological changes, and reduced the systemic immune response. In summary, these findings indicate that TP@LA-Lipo gel offers a comprehensive strategy for effective disease management and improving the quality of life for psoriasis patients. Full article
(This article belongs to the Special Issue Controlled Release Technologies for Localized Drug Delivery)
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28 pages, 1104 KiB  
Systematic Review
Orthodontic Ceramic Bracket Removal Using Lasers: A Systematic Review
by Mateusz Michalak, Sylwia Kiryk, Agnieszka Kotela, Kamila Wiśniewska, Jan Kiryk, Jacek Zbigniew Zborowski, Jacek Matys and Maciej Dobrzyński
J. Funct. Biomater. 2025, 16(4), 123; https://doi.org/10.3390/jfb16040123 - 1 Apr 2025
Viewed by 149
Abstract
Objective: The aim of this systematic review was to evaluate the effectiveness and safety of various laser systems for debonding ceramic orthodontic brackets compared to conventional mechanical removal methods. The primary outcomes assessed included enamel damage, pulp temperature changes, adhesive remnant index (ARI), [...] Read more.
Objective: The aim of this systematic review was to evaluate the effectiveness and safety of various laser systems for debonding ceramic orthodontic brackets compared to conventional mechanical removal methods. The primary outcomes assessed included enamel damage, pulp temperature changes, adhesive remnant index (ARI), and shear bond strength (SBS). Materials and Methods: A systematic search was conducted in November 2024 across the PubMed, Scopus, and Web of Science (WoS) databases following PRISMA guidelines. The initial search yielded 453 records, of which 41 studies met the inclusion criteria for qualitative and quantitative analysis. The risk of bias was assessed using a standardized scoring system, and only studies with accessible full texts were included. Results: The review highlighted significant heterogeneity in laser parameters, measurement protocols, and study methodologies. Among the evaluated lasers, CO2 and Er:YAG were the most frequently studied and demonstrated high efficacy in debonding ceramic brackets while maintaining enamel integrity. Sixteen studies assessing SBS reported a reduction from baseline values of 13–23 MPa to clinically acceptable ranges of 7–12 MPa following laser application. ARI was analyzed in 25 studies, with laser-treated groups exhibiting higher scores (2–3), indicating safer debonding with more adhesive remaining on the tooth surface, thereby reducing enamel damage. Pulpal temperature increases were examined in 23 studies, revealing that most laser types, when used within optimal parameters, did not exceed the 5.5 °C threshold considered safe for pulpal health. However, diode and Tm:YAP lasers showed potential risks of overheating in some studies. Conclusions: Laser-assisted debonding of ceramic orthodontic brackets is an effective and safe technique when applied with appropriate laser parameters. CO2 and Er:YAG lasers were the most effective in reducing SBS while preserving enamel integrity. However, variations in laser settings, study methodologies, and the predominance of in vitro studies limit the ability to establish standardized clinical guidelines. Further randomized controlled trials (RCTs) are necessary to develop evidence-based protocols for safe and efficient laser-assisted bracket removal in orthodontic practice. Full article
(This article belongs to the Special Issue New Trends in Biomaterials and Implants for Dentistry (2nd Edition))
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17 pages, 6065 KiB  
Article
Polydopamine-Coated Copper-Doped Mesoporous Silica/Gelatin–Waterborne Polyurethane Composite: A Multifunctional GBR Membrane Bone Defect Repair
by Mengmeng Jin, Yi Hou and Feiwu Kang
J. Funct. Biomater. 2025, 16(4), 122; https://doi.org/10.3390/jfb16040122 - 1 Apr 2025
Viewed by 130
Abstract
Guided bone regeneration (GBR) membrane has proven to be a fundamental tool in the realm of bone defect repair. In this study, we develop a mussel-inspired composite biomaterial through polydopamine-assisted, combining gelatin–WPU matrix with the ion-release behavior of Cu–MSNs for augmented bone regeneration. [...] Read more.
Guided bone regeneration (GBR) membrane has proven to be a fundamental tool in the realm of bone defect repair. In this study, we develop a mussel-inspired composite biomaterial through polydopamine-assisted, combining gelatin–WPU matrix with the ion-release behavior of Cu–MSNs for augmented bone regeneration. The optimized composite membrane exhibits enhanced mechanical stability, demonstrating a tensile strength of 11.23 MPa (representing a 2.3-fold increase compared to Bio-Gide®), coupled with significantly slower degradation kinetics that retained 73.3% structural integrity after 35-day immersion in physiological solution. Copper ions act as angiogenic agents to promote blood vessel growth and as antimicrobial agents to prevent potential infections. The combined effect of these components creates a biomimetic environment that is ideal for cell adhesion, growth, and differentiation. This research significantly contributes to the development of advanced biomaterials that combine regeneration and infection-prevention functions. It provides a versatile and effective solution for treating bone injuries and defects, offering new hope for patients in need. Full article
(This article belongs to the Section Bone Biomaterials)
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17 pages, 3197 KiB  
Article
Mass Density Characterization of Hydrogel-Based Systems Inoculated with Bacterial Strains and Dose-Response Performance of Escherichia coli Inoculation
by Carolina Salinas Domján, Mauro Valente and Marcelo R. Romero
J. Funct. Biomater. 2025, 16(4), 121; https://doi.org/10.3390/jfb16040121 - 1 Apr 2025
Viewed by 101
Abstract
This study aims to determine the density of two hydrogel-based media, medium with agar-agar and medium with agar-agar and glucose, which are suitable for both irradiation and bacterial growth, considering the presence or absence of Staphylococcus aureus and Escherichia coli strains. The viability [...] Read more.
This study aims to determine the density of two hydrogel-based media, medium with agar-agar and medium with agar-agar and glucose, which are suitable for both irradiation and bacterial growth, considering the presence or absence of Staphylococcus aureus and Escherichia coli strains. The viability of Escherichia coli cell-inoculated systems was also evaluated to explore potential applications in radiation dosimetry within the 0–10 Gy range, using spectrophotometric and bacterial culture methods. Mass density measurements were performed at varying temperatures using two approaches: the first one, based on direct measurements of mass and volume, yielded densities comparable to liquid water, with uncertainties ranging from 9 to 16%, while the second approach, employing Archimedes’ principle (mass in air vs. mass in a liquid of known density), produced more accurate results, with uncertainties between 0.04 and 0.08%, thus proving more reliable for density determinations. Furthermore, the feasibility study of Escherichia coli-inoculated systems for ionizing radiation dosimetry demonstrated a linear spectrophotometric response to radiation doses across the investigated range, particularly for samples stored at 25 °C. The studied systems were characterized in terms of the corresponding growth curve and post-irradiation bacterial survival, supporting their potentiality as reliable ionizing radiation dosimeters. Full article
(This article belongs to the Special Issue Advances in Multifunctional Hydrogels for Biomedical Application)
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16 pages, 4410 KiB  
Article
Tomographic Assessment of Bone Regeneration in Osteochondral Lesion Treated with Various Biomaterials in a Sheep Model Study
by Taulant Goga, Bledar Goxha, Alberto Maria Crovace, Mario Cinone, Luca Lacitignola, Marta Guadalupi and Erinda Lika
J. Funct. Biomater. 2025, 16(4), 120; https://doi.org/10.3390/jfb16040120 - 1 Apr 2025
Viewed by 178
Abstract
Osteochondral defects, involving both articular cartilage and subchondral bone, pose significant challenges to joint function and health due to the lack of spontaneous healing and the risk of long-term degenerative diseases like osteoarthritis. Biomaterials have emerged as important components in the development of [...] Read more.
Osteochondral defects, involving both articular cartilage and subchondral bone, pose significant challenges to joint function and health due to the lack of spontaneous healing and the risk of long-term degenerative diseases like osteoarthritis. Biomaterials have emerged as important components in the development of scaffolds, providing structural support that facilitates tissue growth, integration, and regeneration. This study aims to demonstrate the effectiveness of a tomographic assessment method for optimizing the evaluation of osteochondral regeneration, particularly using Hounsfield units, to enable the evaluation of scaffold integration and tissue regeneration. The sheep model was selected as a model study. Two distinct configurations of biomaterials were utilized in this study: Honey (HMG—Mg doped hydroxyapatite; HWS—wollastonite–hydroxyapatite) and Bi-layer (BWS—wollastonite–hydroxyapatite). The HMG scaffold demonstrated superior integration, reparative tissue quality, and regeneration potential compared to the HWS, BWS, and CTRL groups. The findings underscore the significance of CT assessment as a preliminary method for evaluating hard tissue, such as bone, employing Hounsfield units. Statistical evaluations validated the significant differences in performance, particularly favoring the HMG group. The results of this study underscore the importance of tomographic assessment in evaluation of osteochondral regeneration. Full article
(This article belongs to the Special Issue Advanced Biomaterials for Bone Tissue Engineering)
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19 pages, 5264 KiB  
Article
Fabrication and Characterization of Highly Porous Gyroid Scaffolds Composed of Deproteinized Bone Mineral
by Otoniel Durán Hernández, Vail Baumer, Genesis Marrero, Sreya Karumanchi and David Prawel
J. Funct. Biomater. 2025, 16(4), 119; https://doi.org/10.3390/jfb16040119 - 28 Mar 2025
Viewed by 153
Abstract
Current treatment methods for critical bone defects involve the implantation of large bone grafts, which are limited by tissue availability and failure to heal correctly with high complication rates. Bioengineered scaffolds have emerged, which deploy biodegradable, highly osteoconductive materials in porous structures to [...] Read more.
Current treatment methods for critical bone defects involve the implantation of large bone grafts, which are limited by tissue availability and failure to heal correctly with high complication rates. Bioengineered scaffolds have emerged, which deploy biodegradable, highly osteoconductive materials in porous structures to accommodate the high mass transport requirements of large bone defects. Ideal scaffold biomaterials require a balance between strength, composition, and osteoconduction, a balance which has yet to be discovered. Naturally derived materials like deproteinized bovine bone mineral (DBBM) have seen successful clinical use for decades as bone void fillers, but their granular or putty form lacks the interconnected porosity required to treat large defects. Leveraging the clinical success of DBBM, this paper presents the first fabrication of highly porous scaffolds composed of naturally derived, deproteinized bone mineral, for potential use in large bone defects. Ovine bone mineral powder was prepared from fresh ovine bone, fabricated into a photopolymeric slurry and 3D-printed using a photocasting process into 67% porous gyroid scaffolds. Ovine bone mineral composition, surface microstructure, compressive properties, and failure probability were evaluated and compared to gyroid scaffolds composed of tricalcium phosphate. Both scaffold types were similar, with characteristics in the low range of human cancellous bone. Full article
(This article belongs to the Special Issue Scaffold for Tissue Engineering)
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16 pages, 748 KiB  
Article
Fabrication of a 3D Corneal Model Using Collagen Bioink and Human Corneal Stromal Cells
by Alexander J. Choi, Brenna S. Hefley, Hannah A. Strobel, Sarah M. Moss, James B. Hoying, Sarah E. Nicholas, Shadi Moshayedi, Jayoung Kim and Dimitrios Karamichos
J. Funct. Biomater. 2025, 16(4), 118; https://doi.org/10.3390/jfb16040118 - 28 Mar 2025
Viewed by 268
Abstract
Corneal transplantation remains a critical treatment option for individuals with corneal disorders, but it faces challenges such as rejection, high associated medical costs, and donor scarcity. A promising alternative for corneal replacement involves fabricating artificial cornea from a patient’s own cells. Our study [...] Read more.
Corneal transplantation remains a critical treatment option for individuals with corneal disorders, but it faces challenges such as rejection, high associated medical costs, and donor scarcity. A promising alternative for corneal replacement involves fabricating artificial cornea from a patient’s own cells. Our study aimed to leverage bioprinting to develop a corneal model using human corneal stromal cells embedded in a collagen-based bioink. We generated both cellular and acellular collagen I (COL I) constructs. Cellular constructs were cultured for up to 4 weeks, and gene expression analysis was performed to assess extracellular matrix (ECM) remodeling and fibrotic markers. Our results demonstrated a significant decrease in the expression of COL I, collagen III (COL III), vimentin (VIM), and vinculin (VCL), indicating a dynamic remodeling process towards a more physiologically relevant corneal ECM. Overall, our study provides a foundational framework for developing customizable, corneal replacements using bioprinting technology. Further research is necessary to optimize the bioink composition and evaluate the functional and biomechanical properties of these bioengineered corneas. Full article
(This article belongs to the Collection Feature Papers in Biomaterials for Healthcare Applications)
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11 pages, 1390 KiB  
Article
Promoting Dentin Bridge Formation Through N-Acetyl-L-Cysteine Application in Rat Molar Pulpotomy: An Experimental Study
by Kota Takagi, Koichi Nakamura, Yoshitaka Yoshimura and Yasutaka Yawaka
J. Funct. Biomater. 2025, 16(4), 117; https://doi.org/10.3390/jfb16040117 - 27 Mar 2025
Viewed by 235
Abstract
Pulpotomy is performed when tooth decay reaches the dental pulp or when the crown is fractured due to trauma. Mineral trioxide aggregate (MTA) is commonly used in pulpotomy, but its prognosis can be variable. N-acetyl-L-cysteine (NAC), an antioxidant amino acid, has garnered attention [...] Read more.
Pulpotomy is performed when tooth decay reaches the dental pulp or when the crown is fractured due to trauma. Mineral trioxide aggregate (MTA) is commonly used in pulpotomy, but its prognosis can be variable. N-acetyl-L-cysteine (NAC), an antioxidant amino acid, has garnered attention due to its potential benefits. This study aimed to investigate the effects of MTA and NAC on pulpotomy outcomes. We used Sprague Dawley rat maxillary molars to perform pulpotomy and employed Superbond C&B, MTA, and MTA mixed with NAC (MTA–NAC) for pulp capping. We obtained tissue sections 3 and 7 days postpulpotomy, conducting histological analysis by examining the morphology of pulp tissue and assessing dentin sialophosphoprotein (DSPP) and osteopontin expression levels. At 3 days postpulpotomy, MTA and MTA–NAC reduced the inflammatory response. At 7 days postpulpotomy, dentin bridge formation was observed following MTA–NAC application, and although MTA resulted in DSPP- and osteopontin-positive areas, these areas were more extensive following MTA–NAC application. Given that adding NAC to MTA enhanced dentin bridge formation, MTA–NAC appears to be a superior option for pulp capping. Full article
(This article belongs to the Special Issue Biomechanical Studies and Biomaterials in Dentistry)
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18 pages, 4728 KiB  
Article
Influence of Pontic Length on the Structural Integrity of Zirconia Fixed Partial Dentures (FPDs)
by Tareq Hajaj, Ioana Elena Lile, Ioana Veja, Florina Titihazan, Mihai Rominu, Meda Lavinia Negruțiu, Cosmin Sinescu, Andreea Codruta Novac, Serban Talpos Niculescu and Cristian Zaharia
J. Funct. Biomater. 2025, 16(4), 116; https://doi.org/10.3390/jfb16040116 - 25 Mar 2025
Viewed by 170
Abstract
Objective: This study aims to evaluate the influence of pontic length and design on the fracture resistance of zirconia fixed dental prostheses (FDPs). By assessing different span lengths under controlled mechanical loading conditions, the research seeks to provide insights into optimizing the structural [...] Read more.
Objective: This study aims to evaluate the influence of pontic length and design on the fracture resistance of zirconia fixed dental prostheses (FDPs). By assessing different span lengths under controlled mechanical loading conditions, the research seeks to provide insights into optimizing the structural integrity of zirconia dental bridges. Materials and Methods: A total of 20 zirconia bridges were fabricated and tested in vitro. Ten bridges were designed to replace a single missing molar (tooth 46), with a pontic span of 11 mm, while the remaining ten were crafted for two missing teeth (35 and 36), featuring a longer pontic span of 17 mm. The zirconia frameworks were milled using the Wieland Zenotec® Select Hybrid system and cemented onto metal abutments with Voco Meron Plus QM resin-reinforced glass ionomer cement. The specimens were subjected to occlusal loading using a ZwickRoell ProLine Z005 testing machine at a crosshead speed of 1 mm/min until fracture occurred. Results: The mechanical testing revealed a significant correlation between pontic length and fracture resistance. The mean fracture resistance for three-unit bridges (single pontic) was 3703 N, whereas four-unit bridges (double pontic) exhibited a significantly lower resistance of 1713 N. These findings indicate that increased span length reduces the fracture resistance of zirconia restorations due to higher stress accumulation and reduced rigidity. Conclusions: This study underscores the importance of pontic length and design in determining the fracture resistance of zirconia restorations. Shorter spans exhibit greater structural stability, reinforcing the need for careful treatment planning when designing multi-unit zirconia bridges. By optimizing bridge parameters, clinicians can improve clinical outcomes and extend the longevity of zirconia prostheses in restorative dentistry. Full article
(This article belongs to the Special Issue Technologies and Materials for Application in Dental, Oral and Maxillofacial Engineering)
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20 pages, 43063 KiB  
Article
Intramuscular Reactivity of the Modified Graphene Oxides and Their Bio-Reactivity in Aging Muscle
by Xiaoting Jian, Jiayin Wang, Jijie Hu, Yangyang Li, Qisen Wang, Han Wang, Jingwen Huang, Yu Ke and Hua Liao
J. Funct. Biomater. 2025, 16(4), 115; https://doi.org/10.3390/jfb16040115 - 25 Mar 2025
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Abstract
To enhance the biocompatibility and drug delivery efficiency of graphene oxide (GO), poly(ethylene glycol) (PEG), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), or its triblock copolymer PEG-PHBV-PEG (PPP) were used to chemically modify GO. However, it is still unknown whether non-toxic polymer-modified GO mediates muscle toxicity or triggers [...] Read more.
To enhance the biocompatibility and drug delivery efficiency of graphene oxide (GO), poly(ethylene glycol) (PEG), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), or its triblock copolymer PEG-PHBV-PEG (PPP) were used to chemically modify GO. However, it is still unknown whether non-toxic polymer-modified GO mediates muscle toxicity or triggers intramuscular inflammation. This study aims to investigate the biological reactivity and inflammation/immune response induced by PEG, PHBV, or PPP modified GO when injected into the tibialis anterior (TA) muscle of mice prior to drug loading. The results showed that after muscle exposure, the coating of biocompatible polymers on GO is more likely to provoke muscle necrosis. Muscle regeneration was found to occur earlier and more effectively in muscle treated with hydrophilic PEG-GO and PPP-GO compared to muscle treated with hydrophobic PHBV-GO. When observing the transient muscle macrophage invasion of three modified GOs, PHBV-GO caused severe muscle necrosis in the early stage, induced a delayed peak of macrophage aggregation, and caused severe inflammatory progression. All three kinds of modified GO induced T cell aggregation to varying degrees, but PEG-GO induced early mass muscle recruitment of CD4+ T cells and was more sensitive to cytotoxic T cells. Based on the higher biocompatibility of PPP-GO in muscles, PPP-GO was implanted into the muscles of old or adult mice. Compared to adult mice, aged mice are more vulnerable to the stress from PPP-GO, as demonstrated by a delayed inflammatory response and muscle regeneration. Full article
(This article belongs to the Section Biomaterials for Drug Delivery)
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